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Citations for Qiagen :
851 - 900 of 1798 citations for Human DOK4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... all cloning steps were performed in DH5α carrying plasmid pREP4 (Qiagen) for expression of the Lac repressor ...
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bioRxiv - Plant Biology 2023Quote: ... Plasmid DNA was isolated using the QIAprep Spin Miniprep Kit (Qiagen) and sequence verified by Elim Biopharm.
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bioRxiv - Microbiology 2024Quote: Plasmid DNA was amplified using REPLI-g® Midi Kit (QIAGEN), according to the manufactureŕs instructions ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid DNA was purified using a QIAprep Spin Miniprep kit (Qiagen) or QIAGEN Plasmid Midi kit (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... The Endo-free plasmid maxi kit (#12162, Qiagen, Valencia, CA, USA) was used for plasmid purification.
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bioRxiv - Bioengineering 2024Quote: ... DNA for transfection was purified using EndoFree Plasmid Maxi Kit (Qiagen). For single plasmid transfections ...
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bioRxiv - Bioengineering 2024Quote: ... All plasmids were extracted using QIAprep® Spin Miniprep Kit (Qiagen). The gRNA09 cassette targeting pcaC was built via inverse PCR using back-to-back primers containing the 20-bp spacer ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmids were transfected in COS-7 cells with Effectene (Qiagen, Germany) according to the manufacturer’s protocols for 22 hours ...
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bioRxiv - Biochemistry 2023Quote: ... All constructs were purified using endotoxin-free plasmid extraction kits (Qiagen) and sequenced to verify intact reading frames.
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was extracted using the QIAprep Spin Miniprep Kit (QIAGEN) and constructs were validated by Sanger sequencing (Eurofins Genomics ...
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bioRxiv - Genomics 2019Quote: ... and human genomic DNA (extracted from cells with DNeasy Blood & Tissue Kit, Qiagen). One guide was used with the plasmid ...
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bioRxiv - Neuroscience 2021Quote: Cytokine secretion assays were performed using Human Multi-Analyte ELISArray plate (Qiagen CMEH6321A). IL1β ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were prepared using the QIASeq Human Comprehensive Cancer Panel Kit (Qiagen #333515) and sequenced on an Illumina HiSeq 2500 or NovaSeq 6000 ...
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bioRxiv - Pathology 2019Quote: Total RNA of human podocytes was extracted per manufacturer’s instructions (Qiagen, Germantown, MD) and 1 μg RNA was used for cDNA synthesis (Transcriptor first strand cDNA synthesis kit ...
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bioRxiv - Genomics 2019Quote: We analyzed human and mouse Tug1 cDNA sequences with CLC Genomics Workbench (Qiagen) for open reading frames (ORFs) ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted from human cells with an RNeasy Mini kit (Qiagen). Northern blot was performed as described previously (Tafforeau et al. ...
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bioRxiv - Neuroscience 2022Quote: ... using commercially available primers for human FAAH (CpG Island 100530) (EPHS100530-1A, Qiagen). Methylation-sensitive (EPHS115450-1A ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was extracted from human thyroid tissues using miRNeasy Mini kit (Qiagen) according to the manufacturer’s instructions and as described [26] ...
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bioRxiv - Neuroscience 2023Quote: ... Human rRNA depletion was performed using the QIASeq FastSelect kit (Qiagen, Hilden, Germany). RNA sequencing library preparation used NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s recommendations (New England Biolabs ...
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bioRxiv - Immunology 2023Quote: DNA from human stool samples was extracted with DNeasy PowerSoil Pro kit (Qiagen) according to the manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2022Quote: Total RNA for human TSCs was extracted using RNeasy Micro kit (Qiagen, 74004) with on-column DNase digestion ...
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bioRxiv - Synthetic Biology 2023Quote: ... or primary human cells were extracted using the Qiagen RNeasy Micro Kit (Qiagen). cDNA was produced using the SuperScript IV VILO cDNA Synthesis Kit (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... plasmids were prepared from 5 ml overnight culture using Miniprep kits (Qiagen) and the plasmids were sequenced using M13F-M13R primers (Table S4).
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bioRxiv - Cancer Biology 2021Quote: ... cultured overnight in liquid LB and extracted using Plasmid miniprep kit (Qiagen). Plasmid DNA sequences were checked using Sanger sequencing (Eton Bioscience ...
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bioRxiv - Cell Biology 2020Quote: ... pMSuperCos-ARS1 was isolated from DH5α using a Plasmid Maxi Kit (Qiagen). 100 ug plasmid were digested with 100 U Notl-HF and XbaI (NEB ...
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bioRxiv - Developmental Biology 2021Quote: ... Plasmid DNA was isolated from mutant clones using a standard protocol (QIAGEN). After paired-end Sanger sequencing ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The vectors were isolated using a plasmid maxi kit (Qiagen, Hilden, Germany) and transformed into E ...
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bioRxiv - Synthetic Biology 2022Quote: ... DNA was prepared using the QIAGEN Plasmid Plus Midi Kit (Qiagen 12943) for transfection grade DNA purification ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmids were then isolated using QIAprep Spin Miniprep (Qiagen, cat no 27104), sequenced and used as templates to generate PCR products ...
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bioRxiv - Genomics 2022Quote: ... the cultured bacteria was pooled prior plasmid purification (Qiagen, 12943 or 12963). Four of the aliquots were sampled and plated with serial dilutions after 1 hr recovery to estimate transformation efficiency.
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bioRxiv - Molecular Biology 2020Quote: ... Endotoxin-free plasmid DNA isolation kits were purchased from Qiagen (cat. 12362). Agarose ...
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bioRxiv - Bioengineering 2019Quote: ... Plasmid DNA were prepared using QIAprep Spin Miniprep Kit (Qiagen, Cat. # 27104) and screened by restriction enzyme fingerprinting ...
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bioRxiv - Microbiology 2019Quote: Plasmids were isolated using the QIAprep Spin Miniprep Kit (Qiagen, Valencia, CA). DNA fragments and enzyme reactions were purified with QIAquick gel extraction kit (Qiagen) ...
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bioRxiv - Neuroscience 2019Quote: ... Circular DNA donor plasmids were purified with an endotoxin-free kit (Qiagen).
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bioRxiv - Genomics 2021Quote: ... the S1 and S2 plasmids were purified using a GigaPrep kit (Qiagen). To isolate the insert ...
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bioRxiv - Cancer Biology 2021Quote: ... and plasmids expressing various cofactors (200 ng) using Effectene Transfection Reagent (QIAGEN). After 48 hours ...
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bioRxiv - Bioengineering 2020Quote: ... plasmid DNA was extracted using a QI-Aprep Spin Miniprep Kit (Qiagen), and the purified plasmid DNA was sent to Eurofins Genomics for Sanger sequencing ...
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bioRxiv - Bioengineering 2020Quote: ... and extracted using the EndoFree Plasmid Maxi Kit (Qiagen, Inc., Valencia, CA).
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bioRxiv - Neuroscience 2020Quote: ... confirmed by Sanger sequencing and prepared for injection (QIAGEN, Plasmid Midi Kit).
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bioRxiv - Microbiology 2020Quote: ... Plasmids were routinely isolated using the Qiaprep Spin Miniprep kit (Qiagen, USA), and all primers were purchased from Integrated DNA Technologies (IDT ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmids were recovered from bacteria using the QIAprep Spin Miniprep kit (Qiagen).
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid DNA was purified using QIAGEN HiSpeed® Maxi kit (QIAGEN 12662), eluting in 1 mL TE Buffer ...
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bioRxiv - Biochemistry 2020Quote: ... we took 1 µg of plasmid (typically from a midi-prep (Qiagen)) and digested in a 20 µL reaction with 20 units of BamHI-HF (NEB ...
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bioRxiv - Microbiology 2019Quote: ... Plasmids were routinely isolated using the Qiaprep Spin Miniprep kit (Qiagen, USA), and all primers were purchased from Integrated DNA Technologies (IDT ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were routinely isolated using the Qiaprep Spin Miniprep kit (Qiagen, USA), and all primers were purchased from Integrated DNA Technologies (IDT ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid DNA was extracted using QIAprep®-Spin Miniprep kit (Qiagen, 27104) or Maxiprep (Qiagen ...
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bioRxiv - Biochemistry 2022Quote: ... the cDNA obtained was cloned in plasmid pFP1001 (derived from pQE30, QIAGEN) using BamHI and HindIII restriction enzymes and T4 DNA Ligase (Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid isolation was performed using QIAprep Spin Miniprep Kits (Qiagen, Mississauga, ON). The polymerase chain reaction (PCR ...
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bioRxiv - Cell Biology 2022Quote: Plasmids were isolated from bacteria using the QIAprep Spin Miniprep Kits (Qiagen). HEK293T-Cas9 cells were transfected using Lipofectamine 3000 (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmid DNA was extracted using the QIAprep Spin Miniprep kit (QIAGEN; 27104) and submitted for sequencing at the MRC PPU DNA sequencing and services division ...