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Citations for Qiagen :
851 - 900 of 10000+ citations for Cyclic GMP Direct Chemiluminescent ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Triton X-100 1.2% and lysozyme 20mg/ml) and incubated at 37°C for 1 h and DNA was extracted with the DNeasy blood&Tissue kit (Qiagen, Courtaboeuf, France) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... at 37 °C for 1 h and recovered by using RNeasy MinElute Cleanup kit as recommended by the manufacturer (Qiagen, Hilden, Germany). RNA yields were estimated by employing a Qubit® Fluorometer as recommended by the manufacturer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from cultures of LSUCC0096 that had reached max cell density (~106 cells mL−1) growing in JW1 medium (10) at room temperature using a MoBio PowerWater DNA Isolation kit (QIAGEN, Massachusetts, USA) following the manufacturer’s protocols ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was extracted from the liver of an adult female Limnodynastes dumerilii dumerilii (Fig. 1) using the Gentra Puregene Tissue Kit (QIAGEN, Hilden, Germany) according to manufacturer’s instructions with the following exceptions ...
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bioRxiv - Genetics 2020Quote: ... at least ∼1 × 107 cells were collected by centrifugation and RNA was purified using an RNeasy Mini Kit (Qiagen 74104; Hilden, Germany), including on-column DNase digestion (Cat No ...
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bioRxiv - Microbiology 2020Quote: High-quality genomic DNA was obtained starting from 250 mg of each sample of table 1 using DNeasy PowerSoil Kit (QIAGEN, Hilden, Germany), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and isolated keratinocytes and fibroblasts from the skin and Wound7 (n = 5/each group) (Table 1 and Table S1) by using the miRNeasy Mini kit (Qiagen, Hilden, Germany) and prepared for library construction ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted from up to 1 gram of sediment using a modified version of the Qiagen DNeasy PowerSoil kit (Qiagen, Venlo, Netherlands), as described previously [41 ...
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bioRxiv - Molecular Biology 2022Quote: ... An aliquot of cells was diluted to OD600 0.3 and 500 µL of diluted cells was added to 1 mL of RNAprotect Bacteria Reagent provided in the RNeasy Mini Kit (Qiagen; catalog #74104). Protocols provided in the “RNAprotect Bacteria Reagent Handbook 2015” were used for RNA extraction (“Protocol 1”) ...
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Development of immortalized rhesus macaque kidney cells supporting infection with a panel of virusesbioRxiv - Microbiology 2022Quote: ... Then 1 μl of the cDNA preparation were used for quantitative PCR using a QuantiTect SYBR Green PCR kit (Qiagen, Hilden, Germany) and the Rotorgene Q platform (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: ... Total RNA (1 μg) was used for cDNA synthesis using the Quantitate Reverse Transcription kit for cDNA Synthesis for PCR (Qiagen, Germantown, md). Real-time PCR was performed using SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... or if more than one band was present extracted from 1% TAE gel using the QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany). Nucleotide BLAST (BLASTn ...
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bioRxiv - Microbiology 2024Quote: Bacterial genomic DNA was extracted from 1 mL overnight cultures grown in LB media using a Qiagen DNeasy Blood and Tissue Kit (Qiagen, Germantown, MD) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Genomic DNA was then isolated from 1-5 million isolated PBMCs using the DNeasy Blood and Tissue kit (Qiagen; Cat. No. 69504) following manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNAs containing HIV-1 proviruses were recovered by infecting viruses produced in derivatives of THP-1 or SupT11 cells into SupT11 using DNeasy Blood & Tissue Kits (Qiagen, Cat# 69504). Following DpnI digestion ...
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bioRxiv - Molecular Biology 2023Quote: Total RNAs were prepared from livers of rats subjected to inhalational anesthesia with sevoflurane or desflurane for 6 hours or less than 1 minute using an RNeasy Midi Kit (QIAGEN, Venlo, Netherlands) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Each saliva sample was vortexed and 1) 500-750 μl was pipetted into a Powersoil Bead Tube (MOBIO PowerSoil DNA Isolation Kit, QIAGEN Cat #12888) for DNA extraction within two hours of collection (fresh) ...
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bioRxiv - Genetics 2023Quote: ... We isolated the entire exon 1 of HTT with 115 CAG repeats from an HD patient with UltraRun® LongRange PCR Kit (QIAGEN, 206442) with supplementation of 10% DMSO under the following conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... After incubations cells were pickup in buffer RLT with 1% of β-mercaptoethanol and total RNA purification proceeded following the instructions of RNeasy Plus mini kit (Qiagen, USA). Quality control of total RNA was carried out by spectrophotometric readings of optic density (OD ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from 1 mL of each human plasma sample using the DNeasy Blood and Tissue kit (Qiagen, 69504, Hilden, Germany) according to the manufacturer protocol and recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA sequencing on the pancreatic xenografts in the orthotopic mouse model using PDAC cells (AsPC-1 ± SERPINB3) was also performed following isolation of RNA with the RNeasy Plus Mini Kit (QIAGEN, Venlo, Netherlands). Sequencing was performed on NovaSeq 6000 SP (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... DNA was isolated following the Powersoil DNeasy kit protocol with the addition of a 10 min bead beating step at 50 s-1 (Qiagen, Hilden, Germany). RNA was isolated following the Ribopure Bacteria kit protocol (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: Genomic DNA was extracted from Tak-1 thallus according to the method described in (Tsuzuki et al., 2019) or using the DNeasy Plant Mini kit (QIAGEN, Hilden, Germany).
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bioRxiv - Microbiology 2023Quote: ... we placed filters in 2 mL tubes with beads and 1 mL TE buffer (Qiagen plasmid isolation kit, Qiagen Germantown, MD, USA) and ran them in the tissue lyser at either ½ speed or max speed for 30 sec or 1 min ...
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bioRxiv - Genomics 2024Quote: ... Total RNA was extracted from approximately 1 g of fresh gametophytic tissue using the RNeasy Plant Mini Kit (Qiagen, Valencia, CA, USA).
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bioRxiv - Bioengineering 2024Quote: ... cells were lysed in RLT buffer plus β-mercaptoethanol (1:100) and RNA was extracted using an RNeasy plus mini kit (Qiagen 74134). Reverse transcription into cDNA was performed according to iScript manufacturer instructions (iScript cDNA synthesis kit ...
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bioRxiv - Molecular Biology 2024Quote: ... Products were resolved on a 1% (w/v) agarose gel and gel purified using a QIAquick Gel Extraction kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Eye tissue was suspended in Buffer RLT/eye (1 eye/mouse; 10-18 mice/genotype) and prepared using the RNeasy kit (Qiagen, Germantown, MD). RNA quality was assessed by the 18S/28S rRNA bands in capillary electrophoresis (Bioanalyzer ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR products was resolved in 1% agarose gel and the target band was gel purified using a QIAquick Gel Extraction kit (Qiagen, Hilden, Germany). The PCR products were subsequently subjected to direct Sanger sequencing using G680V-F to for the adjacent G680V – A684G SNPs and V528L Seq2 for the V528L SNP ...
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bioRxiv - Neuroscience 2019Quote: ... Only the neocortical primordium and the commissural plate were dissected and stored in RNA Later (Qiagen) at −20°C until RNA extraction ...
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bioRxiv - Systems Biology 2020Quote: ... Each well in the NAB plate was then washed once with 500 uL Buffer AW1 (QIAGEN) and once with 500 μL of Buffer AW2 (QIAGEN) ...
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bioRxiv - Developmental Biology 2024Quote: mESCs were plated on gelatin-coated plates in N2B27-2i and transfected with FlexiTube siRNAs (Qiagen) using DharmaFECT 1 (Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... and placed in a well of 96-well plate containing 2,5µl of RLT plus buffer (Qiagen). At the end of cell collection ...
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bioRxiv - Systems Biology 2023Quote: ... Each well in the NAB plate was then washed once with 500 μL buffer AW1 (QIAGEN) and once with 500 μL of buffer AW2 (QIAGEN) ...
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bioRxiv - Molecular Biology 2021Quote: ... Sodium bisulfite treatment was carried out using commercially available bisulfite conversion kits (EpiTect Fast Bisulfite Kit and EpiTect Fast LyseAll Bisulfite Kit; Qiagen) according to the manufacturer’s instructions except for the DNA denaturation step and sodium bisulfite reaction time ...
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bioRxiv - Microbiology 2024Quote: ... and the genomic DNA/RNA extractions were conducted using commercial extraction kits (DNeasy Blood & Tissue Kit for DNA or RNeasy Mini Kit for RNA, both from Qiagen) and purified with GeneJET Genomic DNA Purification Kit (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... total RNA was isolated using RNeasy Mini Kit or Micro Kit (Qiagen). Reverse transcription was performed using M-MLV Reverse Transcriptase and random primers following manufacturer’s instructions (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... RNA was extracted using a commercial kit (RNseasy Plant Mini Kit, Qiagen), followed by cDNA synthesis (iSCRIPT ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using standard column-based purification kit (QIAGEN RNeasy Kit, Cat. No. 74004). DNase treatment was applied during the purification to remove any potential DNA contamination ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted using the RNeasy Mini Kit extraction kit (QIAGEN). RNA-less and reverse transcriptase-less reactions were used as controls ...
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bioRxiv - Cancer Biology 2021Quote: ... purified with QIAquick Gel Extraction kit or QIAquick PCR Purification kit (Qiagen), ligated using T4 DNA Ligase (New England BioLabs) ...
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bioRxiv - Genetics 2020Quote: ... Plasmids were prepared using silica-membrane based kit (Plasmid Miniprep Kit, Qiagen) following the manufacturer’s instructions and quantified using Nanodrop Spectrophotometer ...
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bioRxiv - Genetics 2020Quote: ... according to the DNeasy Blood & Tissue kit and DNeasy Micro kit (QIAGEN). Total RNA was extracted from pelleted cells using the Rneasy Mini kit (QIAGEN ...
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bioRxiv - Microbiology 2019Quote: ... using viral RNA isolation kit (QIAmp Viral RNA Mini Kit Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and extracted with Jetgene mini prep kit (QIAprep Spin Miniprep Kit, QIAGEN). The correctness of constructions was verified by sequencing (Eurofins) ...
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bioRxiv - Microbiology 2023Quote: ... QIAprep Spin miniprep kit and QIAquick gel extraction kit (Qiagen, Hilden, Germany) were used according to the manufacturer’s protocol to isolate plasmids and to separate PCR products from agarose gels ...
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bioRxiv - Biochemistry 2023Quote: ... gel extraction kit and PCR clean-up kit was obtained from Qiagen, Hilden ...
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bioRxiv - Biochemistry 2022Quote: ... QIAGEN Plasmid Maxi Kits and QIAprep Spin Miniprep Kit were from QIAGEN. Zymo PUREII Plasmid Midiprep kit (D4200 ...
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bioRxiv - Molecular Biology 2023Quote: ... plasmid DNA preparation (QIAprep Spin MiniPrep Kit and Plasmid Midi Kit, Qiagen) and gel electrophoresis were performed according to the manufacturer’s instructions or using standard protocols (29) ...
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bioRxiv - Cell Biology 2024Quote: DNA was extracted using a commercial kit (DNeasy Blood & Tissue kit, Qiagen). For cDNA ...