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Citations for Qiagen :
851 - 900 of 4272 citations for 6 METHYL 2 OXO 4 THIOPHEN 2 YL 1 2 3 4 TETRAHYDRO PYRIMIDINE 5 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... One half of clarified cell lysate (2 ml) was applied to 200 μl of Ni-NTA Superflow resin (Qiagen) equilibrated with Ni wash buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were harvested after 2 hours of treatment and RNA was isolated with the RNeasy Mini Kit (Qiagen #74104). RNA levels were assessed using qScript™ XLT One-Step RT-qPCR ToughMix® (Quanta Bioscience #95132-100 ...
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bioRxiv - Bioengineering 2021Quote: ... The filtered supernatant was incubated for 2 hours at room temperature with 6ml of Ni-NTA resin (Qiagen, 30210). The column containing the mix of supernatant and resin was washed four times with a washing buffer containing 20mM imidazole ...
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bioRxiv - Genomics 2020Quote: ... PCR based COVID-19 viral genome enrichment was performed using QIAseq SARS-CoV-2 Primer Panel Kit (Qiagen, Germany). A total of 98 primer pairs specific to COVID-19 were used for enrichment where primers pairs were distributed in two pools (Pool-1 and Pool-2) ...
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bioRxiv - Molecular Biology 2021Quote: The AR9 gene 226 was PCR amplified from AR9 genomic DNA and cloned into the pQE-2 vector (QIAGEN) between the SacI and SalI restriction sites ...
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bioRxiv - Microbiology 2021Quote: ... 1.5 mL of samples were collected and spun at 12,000 rpm for 2 minutes and resuspended in 350 µL RNA Protect Bacterial Reagent (Qiagen). Samples were kept on ice from this point forward ...
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bioRxiv - Plant Biology 2022Quote: ... The tissue samples were disrupted and homogenized by adding 2 stainless steel beads (SSB 32) using the TissueLyserII (Qiagen) at 20 Hz for 5 mins ...
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bioRxiv - Microbiology 2022Quote: ... The RNA concentration was measured by reverse transcription digital PCR (RT-dPCR) using reported primers and probe 40,41 on QIAcuity dPCR 2-plex platform (QIAGEN) as detailed in the SI.
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bioRxiv - Microbiology 2023Quote: ... Isopropanol was removed and pellets were rinsed 2 times with 70% ethanol and left dry before solubilization with 35μL of EB buffer (Qiagen). The six samples were pooled together and purified ...
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bioRxiv - Microbiology 2023Quote: ... The aqueous phase was removed and transferred to MaxTract High Density 2 mL tubes (Qiagen Inc, Valencia, CA, USA). Samples were then extracted a second time as described above and the aqueous phase from the repeated extractions for each sample were combined ...
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bioRxiv - Neuroscience 2022Quote: ... Any gene with a Z-score greater than 2 was excluded from analyses.Pathway analysis was performed using Ingenuity Pathway Analysis software (QIAGEN). Genes were inputted with their FPKM (cut off >5 FPKM ...
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bioRxiv - Immunology 2022Quote: ... The separated PBMCs were washed with 2% FBS and sample’s mRNA was extracted using RNeasy Plus Mini Kit (Qiagen).
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bioRxiv - Immunology 2023Quote: ... PCR products were run on a 2% agarose gel and purified using QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany). The obtained TCRβ libraries were quantified using the PerfeCTa-NGS-Quantification Kit according to the manufacturer’s protocol (Quantabio Inc ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL of logarithmic growth phase culture was pelleted by centrifugation and resuspended by vortexing in B1 buffer (Qiagen, Hilden Germany ...
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bioRxiv - Genomics 2023Quote: ... Obtained lists with significantly changed genes (p<0.05, fold-change ≥2) were used in Ingenuity Pathway Analysis (IPA, Qiagen).
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bioRxiv - Immunology 2023Quote: ... pH 6.0) and homogenized using a sterile 5mm stainless steel bead in a Tissue Lyser 2 (Qiagen, Hilden, Germany) for 90 seconds at 1/60Hz ...
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bioRxiv - Immunology 2023Quote: ... 40mM Tris-HCl ph 6.5 for 2 hour at 45°C prior to purification with QIAquick PCR Purification Kit (Qiagen). For ‘input’ DNA ...
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bioRxiv - Immunology 2023Quote: ... The supernatant containing target proteins was passed through a polypropylene column containing 2 ml Ni-NTA resin from Qiagen. After the target protein bound to the Ni-NTA resin ...
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bioRxiv - Microbiology 2023Quote: Total RNA was extracted from 2 g of rhizosphere soil using the RNeasy PowerSoil Total RNA Kit (QIAGEN, Canada) and 0.5 g roots using RNeasy Plant Mini Kit (QIAGEN ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse thyroid tissue was homogenized in buffer RLT using TissueLyser II (Qiagen; 2x 2 min cycles at 30 Hz) and 5 mm stainless steel beads ...
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bioRxiv - Molecular Biology 2023Quote: ... The tubes were shaken for 2×45 seconds at 30 Hz using a Tissue Lyser (TissueLyser II, Qiagen, Germany). After homogenization ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 24,000 g for 30 min and applied to a 2 mL column bed of Ni-NTA resin (Qiagen) for purification by IMAC ...
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bioRxiv - Microbiology 2023Quote: ... The pure genomic DNA was sheared by ultrasound and 2-8 kb fragments were extracted from a 0.7% agarose gel using the Gel Extraction Kit (Qiagen). The gel-recovered fragments were ligated to the linearized PZE12 vector (prime 3,4 ...
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bioRxiv - Microbiology 2023Quote: ... containing a sterile 4.5mm ball bearing and homogenized with 2 rounds of bead-beating (2m30s, 30 beats/s) in a TissueLyserII (Qiagen). The lysate was then transferred to a new tube and 1/5 volume of chloroform (Avantor ...
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bioRxiv - Cell Biology 2023Quote: ... for 1h at 37 °C (2 units per sample) followed by purification with the RNeasy miniElute Cleanup kit (Qiagen).
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bioRxiv - Neuroscience 2023Quote: ... cells were washed 2 times with 1X PBS and RNA was isolated using a RNAeasy Plus Mini Kit (Qiagen).
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bioRxiv - Cell Biology 2023Quote: ... individual organoids were washed 2 times with ice-cold PBS and subsequently dissociated with 350µL of lysis buffer (RLT Buffer, QIAGEN). Samples were stored at - 80°C until they were delivered to USEQ Utrecht Sequencing Facility (Utrecht ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Genomic DNA was extracted from 2 mL of each of the nine overnight cultures (DNeasy Blood & Tissue Kit; Qiagen). Library preparation and sequencing was performed by Novogene Europe (Cambridge ...
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bioRxiv - Cancer Biology 2024Quote: ... Mouse thyroid tissue was homogenized in buffer RLT using TissueLyser II (Qiagen; 2x 2 min cycles at 30 Hz) and 5 mm stainless steel beads ...
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bioRxiv - Systems Biology 2020Quote: ... 3 mL of culture were mixed with 6 mL of RNAprotect bacteria reagent (Qiagen) and processed according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... a 19-mer SPRY2 target sequence (5′-AACACCAATGAGTACACAGAG-3) (QIAGEN) was used and for the control a 19-mer NSi control sequence (QIAGEN ...
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bioRxiv - Cell Biology 2020Quote: ... For Astrin siRNA oligo #367 (5′-TCCCGACAAC TCACAGAGAAA -3′)(Qiagen) was used as described (Thein et al. ...
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bioRxiv - Cell Biology 2023Quote: ... snap frozen samples were homogenized in an Eppendorf tube using a 3-mm Tungsten carbide beads and TissueLyser II system (Qiagen; 4 min at 30 Hz). RNA isolation steps were followed as mentioned above in this section ...
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bioRxiv - Genomics 2019Quote: ... are prepared as follows: 4 µL of Vapor-Lock (Qiagen) is manually added to each well using a multichannel pipette followed by 2 µL of lysis buffer (0.2 µL of 25 µg/µL Qiagen Protease ...
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bioRxiv - Pathology 2021Quote: ... RNA was extracted using RNeasy Mini Kit (Qiagen 741-4) per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... and WNT signaling targets PCR array (Qiagen, PAMM 243ZE-4) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... and incubated with 4 mg/mL RNase A (Qiagen 158922) diluted 1:286 in 2X SSC for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... siRNAs were selected among FlexiTube GeneSolution 4 siRNA sets (Qiagen) and reordered after validation as dTdT-overhanging 19 nt RNA duplexes (Thermo) ...
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bioRxiv - Microbiology 2020Quote: ... 4) Powersoil® Isolation kit (MO Bio Laboratories/Qiagen, Canada) by the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and subsequently normalized to 4 nM using elution buffer (Qiagen) with 0.1% Tween20 (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... containing Protease inhibitor cocktail (PIC, Complete, 1:25) and lysed at 4°C using a TissueLyser LT (Qiagen) on 50 Hz for 2 min ...
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bioRxiv - Biophysics 2021Quote: ... and the bindingPCA plasmid containing the ligand GAB2 or CRIPT fused to DHFR1,2 (pGJJ006 and pGJJ066 respectively) were digested with the same enzymes and purified using the QIAquick Gel Extraction Kit (QIAGEN).
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bioRxiv - Cell Biology 2020Quote: ... RNA was further digested with TURBO DNase overnight at 37°C (10 U per 2 μg of RNA) and purified with the RNeasy MinElute Cleanup Kit (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... TRAF3 KO1 and TRAF3/p100 double KO cells from 2 experimental repeats was isolated using the RNeasy Mini Kit (Qiagen). RNA quality control was performed using a 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... run on a 2% agarose gel and a band of 300bp approximately was cut and gel purified using QIAquick Gel Extraction Kit (QIAGEN). Library concentration was determined with KAPA Library Quantification Kit for Illumina Platforms (Kapa Biosystems) ...
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bioRxiv - Immunology 2022Quote: ... the DNA was extracted from 100 mg mouse fecal samples (9-week-old, 2 weeks after final oral gavage treatment) using QIAamp PowerFecal Pro DNA kit (Qiagen) following the manufacturer’s instruction ...
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bioRxiv - Biophysics 2022Quote: ... Purified protein was diluted in PBS buffer (pH = 7.2) and the fluorescence intensity was recorded at 60 °C in the Rotor-Gene 6600 real-time PCR cycler (Qiagen) for 18 h ...
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bioRxiv - Genomics 2020Quote: ... total RNA was extracted from cells grown in five different salinity treatments (0, 2, 12, 24, 36 parts per thousand) using the RNeasy Plant Kit (Qiagen), and 15 Illumina libraries were prepared using the Kapa mRNA HyperPrep kit and sequenced on the Illumina HiSeq2000 platform at the Beijing Genomics Institute.
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bioRxiv - Genomics 2019Quote: ... Purified products were loaded onto a 2% E-gel and gel extracted using a QiaQuick Gel Extraction kit (Qiagen 28704). The molarity of the gel-extracted PCR product was quantified using KAPA library quant (KK4824 ...
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bioRxiv - Genomics 2020Quote: The commonly up- or downregulated genes in cluster 1 and 2 compared to cluster 0 of untreated MDA-MB-231 cells were subjected to the Ingenuity Pathway Analysis (IPA) (QIAGEN) (Kramer ...