Labshake search
Citations for Qiagen :
8851 - 8900 of 10000+ citations for C Reactive Protein ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... MtDNA was isolated using Dneasy Blood and Tissue kits (Qiagen). qPCR was perfomed on these cytosolic supernatants and whole cell extracts using the following mtDNA primers ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were subsequently isolated using a mini-prep kit (Qiagen) and verified by plasmid digest and sequencing from individual colonies grown overnight in LB broth with appropriate antibiotic selection.
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... Plasmid DNA was extracted (QIAprep Spin Miniprep Kit, 27106X4 Qiagen) and sent for Sanger sequencing (Microsynth).
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bioRxiv - Molecular Biology 2024Quote: ... we purified library DNA using QIAquick PCR Purification Kit (Qiagen) with a final elution volume of 30 μl warm 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... and the RNeasy Fibrous Tissue Mini Kit (Qiagen, Hilden, Germany), respectively ...
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bioRxiv - Microbiology 2024Quote: ... burgdorferi cultures using the RNeasy mini kit (Qiagen, Vanelcia, CA) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2024Quote: ... RNA was manually isolated using the RNeasy Mini kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated using the RNeasy Mini kit (Qiagen), reverse transcribed using QuantiTect Reverse Transcriptase (Qiagen) ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA were extracted using the RNeasy mini kit (Qiagen, 74104) and 500 ng of RNA were reverse transcribed using the RT2 First Strand Kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2024Quote: Total RNA was extracted with RNeasy Mini Plus Kit (QIAGEN) and reverse transcribed with iScript cDNA Synthesis kit (Bio-Rad) ...
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bioRxiv - Developmental Biology 2024Quote: ... Transposed fragments were purified using MinElute Reaction Cleanup Kit (Qiagen). Following purification ...
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bioRxiv - Cell Biology 2024Quote: ... and RNA was extracted and purified using RNeasy kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA was purified using the MinElute PCR Purification Kit (Qiagen) and eluted in 10 μl ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was purified using the RNeasy Mini Kit (74104, Qiagen) and ethanol precipitated ...
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bioRxiv - Microbiology 2024Quote: ... the homogenate was loaded into a RNeasy Mini kit (Qiagen) column and RNA purification proceeded according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was extracted using the miRNeasy Mini kit (Qiagen, 217004) and subsequently reverse transcribed using the mScript II RT kit (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: RNA was isolated using the RNeasy Micro Plus Kit (Qiagen) following the manufacturer’s guidelines in Appendix D of the Qiagen RNeasy handbook ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... RNeasy Mini Kit (74104) was purchased from QIAGEN (Germantown, MD). 10X TAE Buffer and RiboRuler High Range RNA Ladder were purchased from ThermoFisher Scientific (Waltham ...
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bioRxiv - Pathology 2024Quote: Total RNA was purified using a RNeasy micro kit (Qiagen) and used for the preparation of RNA-seq libraries by an Ovation SoLo RNA-Seq Library Preparation Kit (Tecan) ...
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted using QIAamp Viral RNA Mini Kit (Qiagen), and cDNA was produced using a ProtoScript® II Reverse Transcriptase (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was isolated using the miRNeasy mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Total RNA was extracted using miRNeasy Kit (Qiagen, Cat # 217004). Total RNA quality was assessed with the use of Bioanalyzer ...
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bioRxiv - Genomics 2024Quote: ... purified using the RNeasy Mini Kit (Qiagen, Chatsworth, CA, USA), and used to construct RNA-Seq (transcriptome ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was extracted using the DNeasy Blood & Tissue kit (QIAGEN), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were purified using PCR clean-up kits (Qiagen). The inserts and backbones were assembled by NEBuilder HiFi DNA Assembly Cloning Kit in a 10ul reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA pellets were washed 3X with 70% ethanol and hydrated in pre-warmed (37°C) Elution buffer (Qiagen, 10 mM Tris-Cl, pH 8.5. Cat. 19086) and incubated on HulaMixer™ Sample Mixer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA for experiments was obtained using endotoxin-free plasmid purification kits (NucleoBond Xtra Midi EF, Takara, cat. 740420.10 or Qiagen EndoFree Plasmid Maxi Kit, cat. 12362); concentration and purity were assessed with spectrophotometry and agarose electrophoresis.
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bioRxiv - Evolutionary Biology 2020Quote: ... Genomic DNA was extracted for whole-genome sequencing from samples using the MO BIO Ultraclean 96 Microbial DNA kit (now sold as QIAGEN DNeasy UltraClean 96 Microbial kit), following the manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2024Quote: ... the soil and sediment samples were extracted using the RNeasy® PowerSoil® Total RNA Kit and RNeasy® PowerSoil® DNA Elution Kit (QIAGEN, Germany), while the marine and freshwater samples were extracted using the DNeasy® PowerWater® Kit and RNeasy® PowerWater® Kit (QIAGEN ...
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bioRxiv - Cancer Biology 2021Quote: ... His-6X-tagged proteins (30 μg/10 μl beads) in 400 μl of PBS were incubated with Ni-NTA Magnetic Agarose Beads (QIAGEN, Hilden, Germany) at room temperature (RT ...
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bioRxiv - Zoology 2019Quote: ... frozen and stored in liquid nitrogen for extraction of total protein and diene conjugates or placed in QIAzol Lysis Reagent (Qiagen, Hilden, Germany) and frozen in liquid nitrogen for RNA isolation ...
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bioRxiv - Biochemistry 2021Quote: ... The labeled protein in the soluble fraction was purified using Immobilized Metal Affinity Chromatography (IMAC) using standard methods (QIagen Ni-NTA resin). The purified protein was then concentrated to 2 mL and purified by FPLC sizeexclusion chromatography using a Superdex 75 10/300 GL (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... coli as an N-terminal histidine-tagged protein isolated from cell lysate after passage through Ni-NTA agarose (Qiagen, Cat. No. 30210). Bound TTRL55P was then eluted by competition with imidazole ...
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bioRxiv - Microbiology 2022Quote: The recombinant His-TvFACPα full-length protein was produced and purified by a standard protocol as suggested by the supplier (QIAGEN, Hilden, Germany) (48 ...
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bioRxiv - Molecular Biology 2019Quote: ... the cell and molecular functions and canonical pathways of these proteins were identified using Ingenuity® Pathway Analysis (IPA®) (Qiagen, Germany).
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bioRxiv - Genetics 2021Quote: ... The final constructs were transformed into BL21 (DE3) for expression and recombinant Fur/mFur proteins were purified using Ni-NTA agarose (Qiagen, California, USA). The proteins induced by the addition of 1 mM IPTG at 16°C for 12 h ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining 80% of the cell suspension was lysed in 6M guanidine-HCl buffer and His6-tagged proteins were captured on Ni2+-NTA resin (Qiagen; cat. #30210). Pull-down eluates and inputs were separated on SDS-PAGE gels and analyzed by immunoblot ...
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bioRxiv - Plant Biology 2022Quote: ... 5’ and 3’ untranslated regions and open reading frames) for the paralogs were made using the CLC Main workbench 7 (QIAGEN® Aarhus A/S, Aarhus C, Denmark). Sequence alignments were generated for the genomic DNA ...
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bioRxiv - Genomics 2020Quote: ... ~20 ng genomic DNA was amplified using a whole-genome amplification (WGA) kit according to the manufacturer’s instructions (Qiagen REPLI-g Mini Kit, Qiagen, Valencia, CA). The REPLI-g Kit is developed based on multiple displacement amplification (MDA) ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted using the Zymo Quick-DNA Universal Solid Tissue Prep Kit (No. D4069) following the manufacturer’s protocol (DNA from a few samples was also extracted with the Qiagen DNeasy Blood and Tissue Kit, No. 69504). DNA quality was assessed by electrophoresis on 3% agarose gels and DNA concentration was determined by dsDNA HS Qubit Assay (Molecular Probes by Life Technologies ...
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bioRxiv - Genomics 2019Quote: ... using the Zymo Quick-DNA Universal Solid Tissue Prep Kit (No. D4069) following the manufacturer’s protocol (DNA from a few samples was also extracted with the Qiagen DNeasy Blood and Tissue Kit, No. 69504). DNA quality was assessed by electrophoresis on 3% agarose gels and DNA concentration was determined by dsDNA HS Qubit Assay (Molecular Probes by Life Technologies ...
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bioRxiv - Evolutionary Biology 2021Quote: ... dispar we used 5 mm pieces of fin clip from each individual sample for 28-29 individuals per site (at 11 sites) to extract DNA with 96-well DNA extraction kits from Qiagen (DNeasy 96 Blood and Tissue Kit) or Macherey-Nagel (NucleoSpin 96 Tissue) ...
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bioRxiv - Bioengineering 2023Quote: ... Genomic DNA of cells co-transfected with a plasmid expressing sgRNA and another expressing SpCas9 was isolated and purified through a commercially available kit (Qiagen, QIAamp DNA Mini Kit Cat# 51304) as per the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... nerve chain and ovaries of each female was extracted using standard protocols (DNA used for ONT sequencing: Macherey-Nagel NucleoBond HMW DNA kit, DNA used for Illumina sequencing: Qiagen DNeasy 96 Blood & Tissue kit). The long-read sequencing library was prepared using the SQK-LSK109 Ligation Sequencing Kit (ONT ...
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bioRxiv - Plant Biology 2020Quote: ... The resulting DNA was purified using QIAquick PCR Purification Kit (Qiagen). DNA was quantified with a LightCycler 480 (Roche ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was isolated using RNeasy Plant Mini Kit (QIAGEN, Catalog # 74904) and subjected to RNase-free DNase I (Life Technologies ...
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bioRxiv - Plant Biology 2020Quote: ... The resulting amplicons were gel purified (MinElute Gel Extraction Kit, QIAGEN) and assembled by Gibson assembly (see Figure S1 for detailed map) ...
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bioRxiv - Microbiology 2020Quote: ... extracted from the gel using the QIAquick Gel Extraction kit (Qiagen), and Sanger sequenced by Genewiz ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from homogenized tissue using a DNeasy kit (Qiagen) according to the manufacturer’s instructions followed by treatment with RNAse I (Qiagen ...