Labshake search
Citations for Qiagen :
801 - 850 of 964 citations for CTLA 4 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... the Qiagen RNeasy Mini Kit was used according to Qiagen RNA Protect Reagent Handbook Protocols 4 and 7 with Appendix B on-column DNase digestion (Qiagen, Hilden, Germany). The RNA was assessed with UV-Vis spectrophotometry (Denovix DS-11 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Quality of the final library has been evaluated through Qubit 4 and fragments sizes were checked by migration using QIAxcel Advanced Instrument (QIAgen, Hilden, Germany). Fragments ready for sequencing sized between 280bp and 320bp ...
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bioRxiv - Cancer Biology 2024Quote: ... Targeted knockdown was conducted with 100nM ON-TARGETplus smartpool human c-MYC (mixture of 4 individual siRNA) and/or human KLF6-SV1 siRNA (Dharmacon, Lafayette, CO) with HiPerfect (Qiagen, Valencia, CA).
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bioRxiv - Developmental Biology 2020Quote: We isolated RNA from single mouse embryonic hearts at embryonic stage E12.5 by using the RNeasy Kit (#74104; Qiagen) and the RNase-Free DNase Set (#79254 ...
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bioRxiv - Neuroscience 2022Quote: RNA was isolated from snap frozen striatal mouse tissue from 2 independent cohorts of mice according to the manufacturer’s protocol and purified using RNeasy columns (Qiagen). A total of 13 mRNA libraries for Illumina sequencing were prepared using KAPA mRNA HyperPrep Kit (KAPA Biosystems ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated from mouse brains with TRIzol or from selected cells with RNeasy Plus Mini Kit (Qiagen) and reverse-transcribed ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted either from mouse total lung tissue after homogenization using the RNeasy Mini Kit (Qiagen GmbH) or from enriched mouse lung CD4+ T cells (CD4 T cell isolation kit ...
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bioRxiv - Neuroscience 2020Quote: RNA from rat Schwann cell cultures or mouse nerve tissue was extracted using an RNeasy Micro Extraction Kit (Qiagen). RNA quality and concentration was determined after extraction using a nanodrop 2000 machine (Thermo) ...
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bioRxiv - Cell Biology 2021Quote: ... DNA was extracted from blood and mouse lung tissues using a Blood and Tissue DNA kit (Qiagen, Germantown, MD). Rat and mouse DNA were quantified by RT-qPCR using rat- and mouse-specific primers included in Supplementary Table 1 (Walker et al ...
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bioRxiv - Immunology 2022Quote: ... The recombinant plasmids for mouse vaccination were prepared using an EndoFree Plasmid Purification Kit (Cat# 12391, Qiagen, Hilden, USA).
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bioRxiv - Molecular Biology 2021Quote: ... and genomic DNA from MEFs and mouse tissue samples were extracted using the DNeasy Blood and Tissue Kit (Qiagen), according to the manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated from cultured cells or mouse kidneys using the total RNA extraction miRNeasy mini kits (Qiagen). cDNA synthesis was performed using the iScript cDNa synthesis kit (Bio-rad) ...
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bioRxiv - Microbiology 2020Quote: RNA from hNE apical washes and mouse oropharyngeal swabs were prepared using QIAamp® Viral RNA Mini Kit (QIAGEN) and Nucleospin® RNA kit (Macherey-Nagel ...
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bioRxiv - Neuroscience 2022Quote: ... total RNA was isolated from E11.5 wildtype and humanized mouse dorsolateral telencephalon using the RNAeasy Micro Plus Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Mouse lungs were isolated and snap-frozen in liquid nitrogen or suspended in Allprotect Tissue Reagent (Qiagen, Hilden, Germany). RNA was extracted as directed using the Qiagen RNeasy Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA was generated from 0.1 grams of feces from each mouse using the power soil DNA kit (QIAGEN) according to the manufacturer’s instructions except that cells were broken by a bead beater instead of a vortex ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA was isolated from pooled E12.5 CD1 mouse embryos using an RNeasy® Plus Mini Kit (Qiagen, Hilden, Germany) and then used to generate cDNA with a QuantiTect Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Neuroscience 2019Quote: ... and loaded into a RT² Profiler™ PCR Array Mouse Signal Transduction Pathway Finder™ (cat# PAMM-014Z, Qiagen). These plates were then run on an Applied Biosystems model StepOne Plus PCR machine.
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bioRxiv - Physiology 2021Quote: Mouse fetal hearts were individually homogenised using a stainless steel bead in 500μl RLT buffer (RNeasy, Qiagen, Manchester, UK) and 1% β-mercaptoethanol with a TissueLyser II (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA was extracted from a pool of 500 MN per mouse with the microRNeasy kit according to manufacturer’s instruction (Qiagen). RNA was quantified with a Qubit (fluorimeter from Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... and regulatory network construction was performed for each mouse model using Ingenuity Pathway Analysis (IPA, QIAGEN Bioinformatics, version 52912811). Fold-change values for each protein in the PSD proteome was used as input for a Core Analysis in IPA using the following default settings ...
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bioRxiv - Microbiology 2021Quote: Bacterial DNA was extracted from human and mouse feces-derived bacterial supernatant using the DNeasy powersoil kit (Qiagen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... RNA was extracted from the midbrain of each mouse using the RNeasy® Plus Universal Mini Kit (Qiagen, Germany). The reverse transcription was performed using 300 ng of total RNA mixed with 3.8 μM of oligo(dT)20 (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... and RT2 Profiler PCR Array Mouse WNT Signaling Pathway ABI 7900HT Standard Block plates (Qiagen; Cat. No. PAMM-043ZA) were used for qPCR.
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bioRxiv - Microbiology 2023Quote: Bacterial genomic DNA was isolated from mouse fecal samples following the standard protocol from the DNeasy PowerSoil Kit (Qiagen). Sequencing libraries were generated according to methods adapted from Caporaso et al ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse thyroid tissue was homogenized in buffer RLT using TissueLyser II (Qiagen; 2x 2 min cycles at 30 Hz) and 5 mm stainless steel beads ...
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bioRxiv - Molecular Biology 2024Quote: ... For Southern blotting or qPCR total DNA was isolated from mouse tissues using the DNeasy Blood & Tissue Kit (QIAGEN) as previously described(Kühl et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... Mouse thyroid tissue was homogenized in buffer RLT using TissueLyser II (Qiagen; 2x 2 min cycles at 30 Hz) and 5 mm stainless steel beads ...
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bioRxiv - Pathology 2023Quote: Total RNA was isolated from mouse liver tissues according to the manufacturer’s instructions (RNeasy® Mini Kit, 74106, Qiagen). RNA samples were incubated with DNase I (79254 ...
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bioRxiv - Microbiology 2024Quote: Bacterial DNA was extracted from sorted cells and mouse fecal pellets using the AllPrep PowerFecal DNA/RNA kit (Qiagen) as previously described.13 Extracted DNA underwent library preparation and 16S rRNA gene sequencing of the V4-V5 hypervariable region with 515F/926R primers using the Illumina MiSeq PE250 system at the Université de Québec à Montréal (UQAM ...
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bioRxiv - Physiology 2019Quote: ... to remove the cell debris and were stored at −80°C for no longer than 4 weeks until isolation of cf DNA with QIAamp DNA Blood Mini Kit (Qiagen GmbH, Hilden, Germany) and measurement of plasma concentrations of cf n-DNA and cf m-DNA with real time quantitative PCR as described previously3 ...
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bioRxiv - Developmental Biology 2019Quote: The Duke Microbiome Shared Resource (MSR) extracted bacterial DNA from gut and water samples using a MagAttract PowerSoil DNA EP Kit (Qiagen, 27100-4-EP) as described previously (Murdoch et al. ...
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bioRxiv - Microbiology 2019Quote: Genomic DNA (gDNA) extraction was performed using a custom liquid handling protocol based on the Qiagen MagAttract PowerMicrobiome DNA/RNA Kit (Qiagen 27500-4-EP) adapted for lower volumes ...
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bioRxiv - Systems Biology 2020Quote: Total RNA was extracted from hypothalamus and hippocampus (n = 4 per dietary group per tissue; total 32 samples) using an All-Prep DNA/RNA Mini Kit (QIAGEN GmbH, Hilden, Germany). Sample size was based on previous RNA-Seq studies in which findings were validated using qPCR and gene perturbation experiments [6 ...
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bioRxiv - Developmental Biology 2022Quote: ... Genomic DNA from 4-8 microdissected 10 μm FFPE sections were extracted using the QIAamp DNA FFPE Tissue kit (Qiagen, cat. no. 56404) according to manufacturer instructions ...
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Assessment of Human Renal Transporter Based Drug-Drug Interactions Using Proximal Tubule Kidney-ChipbioRxiv - Cell Biology 2022Quote: Total RNA was isolated from cells in the Kidney-Chip (4 individual samples obtained on Day 14) and transwells (4 samples obtained on Day 14) using buffer RLT plus and RNeasy® Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... The tissue powder was then transferred to a 1.5 mL centrifuge tube where 400 μL of 1% PVP-40 Buffer AP1 solution and 4 μL of RNase A were added (Qiagen DNeasy Plant Kit Qiagen Inc, Hilden, Germany). The tube was initially vortexed to homogenize the solution before incubation at 65 °C for 30 minutes with a short vortex every 5 minutes.
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bioRxiv - Microbiology 2021Quote: Cellular total RNA was prepared from 2-4×106 cells for each sample using the RNeasy Plus Micro kit (Qiagen, Hilden Germany, #74034). For qRT-pCR ...
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Macrobdella decora: Old World Leech Gut Microbial Community Structure Conserved in a New World LeechbioRxiv - Microbiology 2019Quote: ... The supernatant was transferred to clean 1.5mL MCF tube and to which 350 μL ethanol at 4’C was added before vortexing for 10 sec and transferring the supernatant to a QIAamp Mini spin column (Qiagen Germantown, MD U.S.A.). The sample was process as recommended in the QIAamp DNA Mini Handbook and eluted with 10 mM Tris-Cl ...
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bioRxiv - Biochemistry 2021Quote: ... Medium was stirred for 1 hour at 4°C in the presence of 5 ml Ni-NTA Agarose beads (Qiagen, Venlo, The Netherlands) washed with 25 mM Hepes-KOH pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: Total genomic bacterial DNA extraction was performed from frozen fecal samples using the Qiagen DNeasy PowerSoil HTP Kit (Cat. No. 12955-4, Qiagen, Valencia, CA, USA). Adequate DNA yield was confirmed using NanoDrop spectrophotometry ...
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bioRxiv - Genomics 2021Quote: ... Quantification of ACE2 transcript levels was performed by preparing total RNA from 2-4×106 cells for each sample using the RNeasy Plus Micro kit (Qiagen, Hilden Germany, #74034). For qRT-pCR ...
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bioRxiv - Molecular Biology 2022Quote: Viral RNA was extracted from serially diluted and the 4 different media SARS-CoV-2 samples using the QIAamp Viral RNA Mini Kit (QIAGEN GmbH, Hilden, Germany). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... exposed to 0.4 nM and 4 nM of β-estradiol for 10 days) Vagina Chips using QIAzol lysis reagent (Qiagen, cat. no. 79306). Complimentary DNA was synthesized using a SuperScript VILO MasterMix (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... The soluble fraction was separated from debris by centrifugation (9000 g, 4°C) and incubated for 60 minutes with Ni-NTA beads (Qiagen, cat. no. 30230) pre-equilibrated in binding buffer ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted from the hippocampus and cortical regions of Ctrl WT and cKO mice (n=4 per group) using the RNeasy kit (Qiagen, Valencia, CA, USA) method (see location of cortical areas 1 and 2 in the Supplementary Figure 1) ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was extracted from dolphin oral samples and 32 negative (PBS) controls using the DNeasy UltraClean 96 Microbial Kit (Qiagen Cat. #10196-4) following manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: Lyophilized mouse hearts were homogenized in RIPA buffer supplemented with protease and phosphatase inhibitors (Pierce) using a TissueLyser II apparatus (Qiagen), followed by sonication and centrifugation (10 min at 12,000 rpm at 4°C) ...
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bioRxiv - Physiology 2021Quote: 150-200 islets from each mouse islet preparation were washed with PBS and used to generate RNA samples via the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Recombinant protein was blotted with anti-His antibody using the same method: 1:2000 mouse anti-tetra-His IgG (Qiagen); 1:1500 goat anti-mouse IgG-HRP (Dako) ...