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Citations for Qiagen :
801 - 850 of 1132 citations for Absent In Melanoma 2 AIM2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The soluble extracts were applied to 2-ml columns of nickel-nitrilotriacetic acid- agarose (Ni-NTA) (QIAGEN catalog no. 30210) that had been equilibrated with lysis buffer without protease inhibitors ...
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bioRxiv - Biochemistry 2021Quote: ... Ten-fold serial dilution of the SARS-CoV-2 stock were prepared using PBS and viral genomic RNA extracted using the QIAamp viral RNA mini extraction kit (Qiagen) according to the manufacturers spin protocol and the recommended 5.6 μg polyA carrier RNA/140 μL virus sample ...
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bioRxiv - Biochemistry 2021Quote: ... The clarified extract was supplemented with 10 mM imidazole and rotated for 2 hours at 4 °C with 1 ml of Ni-NTA beads (Qiagen). Bound protein was eluted with 10 CV of buffer L / 200 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... and 675 μL PCR products for control cells and 320 μL were applied to the 2% agarose gel for purification using QIAquick Gel Extraction Kit (QIAGEN). The PCR products of control and sort2 were concentrated by using Amicon Ultra-0.5 10K (UFC501096 ...
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bioRxiv - Neuroscience 2021Quote: Fluorophore TYE665 labeled locked nuclear acid (LNA) (C4G2)6 and (CCG)8 probes (Supplemental table 2) were synthesized by Qiagen. The FISH protocol was adapted from (DeJesus-Hernandez et al ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells seeded into 100 mm dishes were transfected at a confluence of 60 % with 2 µg of cDNA encoding PTH1RFRET with Lipofectamine 3000 Transfection Reagent Kit (Qiagen), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was further digested with TURBO DNase overnight at 37°C (10 U per 2 μg of RNA) and purified with the RNeasy MinElute Cleanup Kit (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Genomics 2021Quote: We began the assembly by first mapping long reads to the S288c reference genome (version R64-2-1) using CLC Genomics Workbench (Qiagen)(Fig ...
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bioRxiv - Genetics 2020Quote: ... 2 ml of peripheral blood was used for germline DNA extraction using the QIAamp DNA Blood midi kit (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The final library sample was purified from a 2% agarose gel with silica beads (QIAEX II Gel Extraction Kit, Qiagen). 125bp paired-end sequencing was run on an Illumina HiSeq2500 sequencer at the CRG Genomics Core Facility.
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bioRxiv - Microbiology 2021Quote: ... up to 200 mg of tissue was disrupted in TRIzol (Lifetechnologies) with 2 x 5 mm stainless steel beads using the TissueLyser (Qiagen) for 3 minutes at 25 r/s twice ...
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bioRxiv - Immunology 2020Quote: SARS-CoV-2 ELISA was developed in-house using His tagged proteins bound on Ni-NTA HisSorb Strips or Plates (Qiagen). ELISA assay on mouse sera were performed with His-tagged SARS-CoV-2 full length Spike protein (produced in baculovirus ...
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bioRxiv - Immunology 2020Quote: RNA was isolated from individual pools of 1×104 to 2×104 double-sorted SLAM cells using an RNEasy Micro kit (Qiagen). RNA was quantified and quality checked using an Agilent Bioanalyzer 2100 (Agilent) ...
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bioRxiv - Microbiology 2020Quote: ... up to 200 mg of tissue was disrupted in TRIzol (Lifetechnologies) with 2 x 5 mm stainless steel beads using the TissueLyser (Qiagen) for 3 minutes at 25 r/s twice ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells in 6-well plates were transfected with 2 μg pIRES2-eGFP or Trim-HA-NUP153 derivatives using Effectene (Qiagen). At 24 h post-transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA concentration and purity were measured by a spectrophotometer and 2 ug were used to synthesize cDNA by QuantiTect Reverse Transcription kit (Qiagen). Real-time amplification was performed in a Mastercycler EP Realplex (Eppendorf ...
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bioRxiv - Microbiology 2021Quote: ... Mosquito bodies and legs and wings were put into a 2 ml round-bottom tubes containing 250 ml of PBS and a steel ball (Qiagen). Samples were homogenized using a TissueLyser (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... in Tris/HCl/Ca+2 buffer was subjected to extraction in house using the silica method or QIAamp viral RNA mini kit (Qiagen) according to the manufacturer’s guidelines.
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bioRxiv - Molecular Biology 2021Quote: ... The specimens were gently and quickly homogenized using a BioMasher II homogenizer (Funakoshi) and mixed with 2 mL of Buffer G2 (QIAGEN), including 200 µg/mL RNase A and 50 µL proteinase K (20 mg/mL) ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were clarified by centrifugation at 24,000g at 4°C for 20 min and passed through 2 mL of Ni-NTA nickel resin (Qiagen, 30250) pre-equilibrated with wash buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... for a total of 6 min (2 x 3 min with 1 min on ice in between homogenisations) at 50 Hz using a TissueLyser LT (Qiagen). Thereafter ...
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bioRxiv - Molecular Biology 2020Quote: ... 50uL of the dounce homogenized sample was labelled as “input” and added to 350uL RLT buffer with 1% 2-mercaptoethanol (Qiagen), followed by RNA extraction ...
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bioRxiv - Immunology 2021Quote: ... Amplification was confirmed by running samples on a 2% agarose gel prior to PCR Clean up (QiaQuick PCR Purification kit, Qiagen). Samples were Sanger sequenced (Source Bioscience ...
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bioRxiv - Immunology 2021Quote: Viral RNA was isolated from rhesus macaque plasma from 2 weeks post-SIV infection using an Ultrasense Viral RNA kit (QIAGEN) and cDNA was reverse transcribed using the Applied Biosystems High Capacity cDNA synthesis kit (Thermo-Fisher) ...
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bioRxiv - Immunology 2020Quote: ... RT-qPCR was performed with a primer set targeting partial regions of the ORF1b gene in the SARS-CoV-2 virus using the QIAGEN OneStep RT-PCR kit (Qiagen) as previously reported (51) ...
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bioRxiv - Genetics 2021Quote: ... frozen and lyophilised in a 2 ml 96 deep-well plate following which the samples were ground in the TissueLyser II (QIAGEN) using a steel ball in each well for 4-6 minutes at a frequency of 25 Hz ...
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bioRxiv - Genetics 2019Quote: ... in each well of 8-stripped PCR tubes by shaking with a zirconia bead (2 mm in diameter, Nikkato, Japan) in TissuLyser II (Qiagen) for 30 s at 30 Hz ...
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bioRxiv - Genetics 2019Quote: ... A 5 ul sample was analyzed by electrophoresis on 2% agarose gel and appropriately-digested samples were first purified by PCR purification kit (Qiagen), eluted with 50 ul of 1x NEB Buffer 3 and treated with 10 units of CIP (NEB ...
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bioRxiv - Genomics 2021Quote: ... All plugs were incubated twice (2 hours incubation followed by an overnight incubation) at 50 °C with freshly prepared 167 μl Proteinase K (Qiagen) in 2.5 ml lysis buffer (BioNano Genomics ...
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bioRxiv - Genomics 2020Quote: ... aseptic LS007 shoot tissue was ground under liquid nitrogen using a mortar and pestle and resuspended in 2 mL of AP1 (Qiagen) with 20 µl of RNase I (Qiagen) ...
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bioRxiv - Genomics 2020Quote: ... and Consensus Sequence generation RNA was extracted from eight respiratory samples positive for SARS-CoV-2 using the QIAmp Viral RNA Mini kit (Qiagen). Sequencing was performed as described previously (11) ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR products with sizes from 150 bp to 300 bp were extracted from 2% agarose gel using MinElute Gel Extraction Kit (Qiagen).
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bioRxiv - Biochemistry 2021Quote: ... and early stationary phase (OD = 1.0-1.3).1 mL sample of each culture was directly transferred to 2 mL RNAprotect cell reagent (Qiagen, Hilden, Germany). The samples were vortexed 5 sec ...
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bioRxiv - Biophysics 2022Quote: ... The protein was eluted with 3C protease73 and subsequently incubated at 4°C for 2 h with 4 mL of Strep-tactin Superflow Plus beads (Qiagen) pre-equilibrated with buffer E ...
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bioRxiv - Developmental Biology 2022Quote: ... run on a 2% agarose gel and a band of 300bp approximately was cut and gel purified using QIAquick Gel Extraction Kit (QIAGEN). Library concentration was determined with KAPA Library Quantification Kit for Illumina Platforms (Kapa Biosystems) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... mosquitoes were homogenized in 1.7 mL tubes with 50 μL of water and approximately ten 1.0 mm diameter zirconia beads (Thistle Scientific 11079110zx) by shaking at 30 Hz for 2 minutes in a Tissue Lyzer II homogenizer (Qiagen). DNA was extracted using a PureLink Genomic DNA Mini kit (Thermofisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... 32 μl Master Mix containing 30 μl MDA reaction buffer and 2 μl Phi29 polymerase (REPLI-g UltraFast Mini Kit; Qiagen) were added ...
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bioRxiv - Genomics 2022Quote: ... RNA (3 replicates each of V or E2 treated samples from donor 1 and donor 2) was DNAse treated and cleaned up using the RNeasy Mini kit (Qiagen) or the RNA Clean and Concentrator 5 kit (Zymo ...
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bioRxiv - Microbiology 2022Quote: ... The tubes were weighed again (wa) after sample collection and the samples were homogenized for 2 min at 25 Hz using a TissueLyser from Qiagen. At 4 d.p.i ...
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bioRxiv - Plant Biology 2023Quote: Two-week-old Arabidopsis seedlings of Col-0 wild type and trb1/2/3 triple mutans were used for DNA extraction using DNeasy Plant Mini Kit (QIAGEN). A total of 500 ng DNA was sheared with Covaris S2 (Covaris ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was extracted from 2 mm root tips of 5 DAG seedlings using the RNeasy Plus Micro Kit (cat. No. 74034 QIAGEN). Prior to cDNA synthesis ...
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bioRxiv - Physiology 2022Quote: Assessment of differential expression was conducted on the mapped reads of the 2 previously mentioned pipelines using 2 methods for comparison: the EDGE method with default settings in CLC Genomics Workbench 12 (QIAGEN) and EdgeR (version 3.34.0) ...
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bioRxiv - Neuroscience 2022Quote: ... Pathway analysis was performed for genes 2-fold up- or down-regulated with p-value < 0.05 using Ingenuity Pathway Analysis (IPA) software (Qiagen, USA).
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... The mixture was incubated at 16°C for 2 h and the resulting double-stranded DNA was purified using the MinElute PCR Purification kit (Qiagen) with 20μl EB for elution ...
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bioRxiv - Systems Biology 2022Quote: 2 × 109 cells pretreated with TRIzol reagent were used for preparing RNA reference materials using RNeasy Maxi kit (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... commercially available cDNAs originating from various human tissues were purchased from TaKaRa (detailed sample information is given in Table S3) and 2 ng of cDNA were subjected to quantitative PCR (qPCR) analysis (Rotor-Gene Q, Qiagen), as recommended by the manufacturers ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: 3×106 cells from the day 8 of CD34+ HSPC and day 6 of HUDEP-2 erythroid differentiation were used for total RNA using RNeasy Mini Kit (Qiagen). For reverse transcription using Primescript RT reagent kit (Takara Bio Inc.) ...
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bioRxiv - Immunology 2022Quote: Total RNA was prepared from 1-2*106 sorted naïve (CD25− CD44lowCD62high) or effector (CD25− CD44highCD62low) CD4+ T-cells using RNeasy Mini Kit (Qiagen Inc.) as described in manufacturer’s protocol including an on-column digestion step (RNaseFree DNase Set ...