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Citations for Qiagen :
751 - 800 of 1031 citations for Recombinant Human GC Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The supernatant lysates were purified on Ni-NTA agarose beads (for His6-tagged proteins, Qiagen) or Glutathione High Capacity Magnetic Agarode Beads (for GST-tagged proteins ...
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bioRxiv - Cell Biology 2023Quote: All constructs for bacterial protein expression were cloned in the pQE plasmid (Qiagen, Valencia, CA). WT and mutant TTC1 were cloned downstream of a His14-bdSUMO-GFP tag ...
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bioRxiv - Immunology 2023Quote: ... the proteins were purified by Ni-NTA affinity chromatography using Ni-NTA agarose beads (Qiagen) and gel filtration with a HiLoad 16/600 Superdex 75 pg column in buffers containing 20 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants containing soluble proteins were applied to a 5 mL NiNTA-agarose gravity column (Qiagen) pre-equilibrated with lysis buffer ...
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bioRxiv - Biophysics 2024Quote: DR2539 truncated protein was initially screened by the following commercial crystallization screens: Classics Suite (Qiagen), Salt grid (Hampton Research) ...
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bioRxiv - Genetics 2024Quote: ... Proteins were harvested 60 h after infection and purified with Ni-NTA Superflow resin (Qiagen), anion-exchange chromatography (Source 15Q ...
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bioRxiv - Biophysics 2024Quote: 6xHis-SUMO-Cys-GAF-DBD protein was first purified on Ni-NTA agarose (Qiagen 30210). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was purified by incubation with nickel-nitriloacetic acid (Ni-NTA) agarose resin (Qiagen) for 3h at 4 °C ...
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bioRxiv - Neuroscience 2019Quote: ... total RNA from magnetically purified human NDC, PSEN1M146L, and PSEN1A246E (replicates, n = 4) hiPSC-derived neurons was prepared using RNeasy Plus Micro Kit (Qiagen Cat. 74034) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: Total RNA from magnetically purified NDC, PSEN1M146L, and PSEN1A246E (replicates, n = 3) human iPSC-derived neurons was prepared using miRNeasy Micro Kit (Qiagen Cat. 217084), based on manufacturer’s procedures ...
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bioRxiv - Neuroscience 2019Quote: Total RNA from magnetically purified NDC, PSEN1M146L, PSEN1A246E, and PSEN1H163R (replicates, n = 3) human iPSC-derived neurons was prepared using RNeasy Plus Micro Kit (Qiagen, Cat. 74034) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Genomic DNA was extracted from 2×105 primary human lung fibroblasts harvested in passage 3 using QIAamp Micro Kit (Qiagen, Hilden, Germany) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from cultured from human bronchial epithelial cells / mice right lung tissues and purified using the Qiagen AllPrep DNA/RNA Mini Kit (Qiagen, Hilden, Germany), supplemented with the Proteinase K (Qiagen ...
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bioRxiv - Genomics 2019Quote: ... we extracted whole blood DNA from all individuals included in the RNA sequencing data using Gentra® Puregene® for human whole blood kit (QIAGEN) and MagAttract® HMW DNA kit (QIAGEN ...
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bioRxiv - Genetics 2019Quote: Total RNA from human cell lines (PTC-05, HepG2 and HEK-293T) was extracted using spin columns with the RNeasy Mini Kit (QIAGEN, GmbH, Germany) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... The expression of the BCL-2 anti-apoptotic genes in the NPC cell lines were accessed using the custom RT2 Human Apoptosis Profiler PCR array (Qiagen, Hilden, Germany). To delineate the contribution of MCL-1 for cell survival ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Total RNA from Amborella generative cells and sperm cells was extracted according to the “Purification of total RNA from animal and human cells” protocol of the RNeasy Plus Micro Kit (QIAGEN, Hilden, Germany). In brief ...
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bioRxiv - Immunology 2020Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was carried out by using the ‘Human Innate and Adaptive immune Response’ kit (Qiagen, Hilden, Germany) to assess the expression of genes/mRNA ...
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bioRxiv - Microbiology 2023Quote: ... 293A cells were transfected with the plasmid of pCDNA3.1-ACE2 (human)-3×FLAG (MiaoLing Plasmid Platform, China) using polyfect (QIAGEN, West Sussex, UK) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from FACS sorted mouse brain cells stabilized in RNAprotect buffer according to the “Purification of total RNA from animal and human cells” protocol of the RNeasy Plus Micro Kit (QIAGEN, Hilden, Germany). In brief ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from 1 mL of each human plasma sample using the DNeasy Blood and Tissue kit (Qiagen, 69504, Hilden, Germany) according to the manufacturer protocol and recommendations ...
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bioRxiv - Microbiology 2023Quote: Microbiome DNAs were extracted from the human samples on the same day of collection using the microbiome-specific kit (QIAamp DNA Microbiome Kit; Qiagen, Hilden, Germany). The DNA extraction was performed according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Subsequent targeted sequencing of 160 cancer-related genes was performed using a QIAseq Human Comprehensive Cancer Panel v2 (Qiagen Inc, Valencia, CA) (Table S2) ...
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bioRxiv - Cell Biology 2023Quote: ... Key genes involved in the regulation and enzymatic pathways of fatty liver were simultaneously assayed with the RT2 Profiler PCR Array Human Fatty Liver (PAHS-157ZC-6) (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions and analyzed with the Data Analysis Center (QIAGEN Hilden ...
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bioRxiv - Molecular Biology 2023Quote: ... A total of 1 ng of RNA from microdissected human pancreatic islets and from EndoC-βH1 was used to generate cDNA libraries using QiaSeq miRNA library kit (Qiagen, Hilden, Germany) following manufacturer’s instructions (see ESM Methods).
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bioRxiv - Physiology 2024Quote: Total RNA was isolated from 50 mg of frozen human myocardial LV tissue samples using the miRNeasy Mini Kit (Qiagen: Hilden, DE). To remove all DNA ...
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bioRxiv - Neuroscience 2024Quote: The expression of 84 PPAR target genes was assessed using the Human PPAR Targets RT2 Profiler PCR Array (Qiagen; 384-well format) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR array for human cell cycle (Cat#PAHS-020Z) and oncogenes and tumor suppressor genes (Cat#PAHS-502Z) was purchased from Qiagen (Valencia, CA, USA), and was used according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... To profile the expression of genes related to p53-mediated signal transduction based on the RT² Profiler™ PCR Array Human p53 Signaling Pathway (#330231, PAHS-027ZC, Qiagen, Hilden, Germany), reverse transcription and qPCR were performed using the RT2 First Strand Kit (#330401 ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from the mouse cells/tissues and human cell lines using the RNeasy® Mini Kit (Qiagen Inc., Germantown, MD). For human platelets and MEG-01 cells ...
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bioRxiv - Immunology 2020Quote: ... The clarified supernatant containing the target protein was incubated with pre-equilibrated Ni-NTA resin (Qiagen) for 30 min at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... the proteins were transferred to nitrocellulose membranes before being revealed with anti-Histidine monoclonal antibodies (Qiagen). Immune complexes were detected with anti-rabbit peroxidase-conjugated secondary antibodies (Promega ...
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bioRxiv - Molecular Biology 2021Quote: Proteins were purified from Sf21 insect cells by affinity purification with Ni-NTA agarose (Qiagen, 30230) and ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein purification was performed using Ni+2-NTA agarose affinity chromatography according to standard protocol (Qiagen).
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bioRxiv - Genomics 2021Quote: ... Total RNA was isolated from tumor samples using the AllPrep DNA/RNA/Protein Mini Kit (QIAGEN) according to standard protocol on the QiaCube (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... Insoluble material was removed by centrifugation and 6xHis-tagged protein was purified using Ni-NTA (Qiagen) and gravity chromatography ...
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bioRxiv - Systems Biology 2021Quote: ... PBMCs were thawed on ice and then lysed to protein fraction using Allprep Spin Columns (Qiagen) according to the manufacturer’s instructions with the QIAshredder lysis option ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant containing hexahistidine-tagged proteins was passed over a column of Ni2+-NTA matrix (Qiagen) at 0.5 mL matrix / 1 L of culture ...
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bioRxiv - Plant Biology 2021Quote: ... HIS-tagged proteins were purified by metal-affinity chromatography using Ni-NTA resins (Qiagen, Hilden, Germany). Protein concentration was estimated by the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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STARCH SYNTHASE 4 is required for normal starch granule initiation in amyloplasts of wheat endospermbioRxiv - Plant Biology 2021Quote: ... Denaturing purification of the protein with urea was carried out using the Ni-NTA Agarose (Qiagen). Immunisation of rabbits was carried out at Eurogentec ...
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bioRxiv - Microbiology 2021Quote: ... The LuxT-6xHis protein was purified from the clarified supernatant by Ni-NTA Superflow resin (Qiagen). Following washes with lysis buffer containing 20 mM Imidazole ...
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bioRxiv - Cancer Biology 2021Quote: ... washed again and proteins were visualized by SuperSignal™ West Dura Extended Duration Substrate (Qiagen #34076)
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bioRxiv - Biochemistry 2022Quote: ... Protein extract was subjected to IMAC by incubation with 2 ml Ni-NTA resin (Qiagen, Germany) per 50 ml of extract ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extract (1 mg) was incubated with His6-RabD2c (1 μg) and Ni-NTA Agarose (QIAGEN) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tagged protein was purified by affinity chromatography on Ni-NTA agarose beads (Qiagen 30230) and cleaved on beads with TEV protease o/n at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... The protein sequences were aligned by MUSCLE [76] and visualized by CLC Genomic Workbench 8.5.1 (Qiagen). Subsequently ...
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bioRxiv - Immunology 2022Quote: ... Protein expression was performed using standard protocols and purification was performed using Ni-NTA Agarose (Qiagen). Recombinant RelMtb protein (87 kDa ...
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bioRxiv - Plant Biology 2019Quote: ... The protein was purified under denaturing conditions on Ni2+-nitrilotriacetic acid matrix (Ni-NTA Agarose, Qiagen) and used to raise polyclonal antibody in rabbits (Eurogentec) ...
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bioRxiv - Biochemistry 2019Quote: ... 20 mM imidazole] and BiP proteins were bound to 80 µl Ni-NTA agarose beads (Qiagen) for 30 min at 25 °C in the presence of 0.01% Triton X-100 ...
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bioRxiv - Neuroscience 2020Quote: ... The His-tagged target protein was purified by Ni-NTA gravity-flow chromatography (Qiagen, Hilden, Germany). The eluted protein was subjected to MonoQ 10/100 GL (GE Healthcare Lifesciences ...