Labshake search
Citations for Qiagen :
751 - 800 of 890 citations for Proteasome 26S Subunit Non ATPase 10 PSMD10 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The high-quality reads were de novo assembled using the short reads assembly program Trinity version 2.3.2ref56 as well as a CLC Genomics Workbench version 10 (Qiagen, Carlsbad, CA, USA). The Trinity assembly yielded a total of 74,151 predicted ‘genes’ with their isoforms totaling 171,567 predicted ‘transcripts’ (i.e. ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 10-20 mg of silica-dried stem or leaf tissue was ground with a Qiagen TissueLyser II (Qiagen, Melbourne, Vic., Australia). DNA was extracted using the DNeasy 96 Plant Kit or DNeasyPlant Mini Kit (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... 65-79*SE or 65-79*PE (100 μg/mL) with or without IFN-γ (10 ng/mL) using the RNeasy mini kit (Qiagen) according to the vendor instructions ...
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bioRxiv - Immunology 2021Quote: At least 10,000 sorted cells or 10 μL of whole blood sample were collected for genomic DNA extraction using the DNeasy Blood & Tissue Kit (Qiagen, #69504). 3 μL of genomic DNA was used as template to amplify the sgRNA target region ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Total RNA was extracted from 10 samples (5♀♀, 5♂♂) using RNeasy Micro kits (QIAGEN K.K., Tokyo, Japan) following the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... The sample was then eluted in buffer AE (10 mM Tris-Cl; 0.5 mM EDTA; pH 9.0; Qiagen Inc., Courtaboeuf, France). DNA purity was assessed with a NanoDrop spectrophotometer based on the 260/280 and 260/230 O.D ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The aqueous fraction of the Trizol lysate was precipitated with ethanol and was resuspended in 10 mM Tris pH 8.0 before purification using the QIAamp viral RNA mini kit (QIAGEN, Manchester, UK) in the absence of carrier RNA ...
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bioRxiv - Microbiology 2021Quote: ... 1.5mL of the culture was pelleted (13500 rpm for 10 min) and used for the extraction of DNA with DNeasy Blood and Tissue Kit (Qiagen, Germany). For eluting nucleic acids ...
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bioRxiv - Genomics 2020Quote: BAC clones were extracted from each of these primary pools using either the Qiagen Large-construct Kit (10) (12462, Qiagen, Germany) or Omega BAC/PAC DNA Maxi Kit (D2154-02 ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from MCF10AER/vSrc cells (Control and treated with 10 nM D-Alanine for 4 h or 24 h) using RNeasy Kit (Qiagen, #74104) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: DNA was extracted from 1×10^6 CD4 Th cells/ replicate using the Qiagen DNA mini kit according to manufacturer’s instructions (Qiagen Hilden, Germany). DNA was extracted from 10ng of mouse brain tissue as previously described13 using NEB Monarch High molecular weight DNA extraction kit according to manufacturer’s instructions (NEB Cat #T3050).
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bioRxiv - Plant Biology 2022Quote: ... pods from 8-10 different plants were pooled and RNA was isolated using RNAeasy plant mini kit (Qiagen, Germantown, MD, USA). RNA libraries were prepared using standard Illumina protocols and RNA sequencing was performed using NovaSeq 6000 PE150 by Novogene co ...
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bioRxiv - Physiology 2023Quote: ... 1 µg of RNA isolated from human islets or 10 µL of exosomal RNA was reverse transcribed using the miRScript II kit (Qiagen, Germany), following manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... buffer containing 100 mM Tris-HCl (pH 8.5), 10 mM TCEP, and 40 mM CAA and crushed using zirconia beads (Tomy, Tokyo, Japan) and TissueLyser (Qiagen, Hilden, German). Proteins were inactivated at 95 °C for 5 min ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was isolated from 10-d-old seedling roots (40 for each line) using the RNeasy Plant Mini Kit (Qiagen, Germany). cDNA was synthesized using TOPscriptTM RT DryMIX (dT18 ...
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bioRxiv - Genomics 2023Quote: ... The sample was then centrifuged at 15000 rpm for 10 min and the pellet was resuspended in 100 μl buffer EB (Qiagen, 19086). For each sample of a 96-well plate ...
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bioRxiv - Plant Biology 2022Quote: ... Technical duplicates of gene-specific products were synthesised in 10 µl reactions using the Rotor-Gene SYBR Green PCR kit (Qiagen, UK) on a Rotor-Gene 6000 Real-Time PCR machine (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated from 9 AAA and 10 PAD patients’ serum using the miRNeasy Serum/Plasma Advanced Kit (Qiagen, Hilden, Germany), following in principle the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... callus and contralateral intact bone were removed as described previously (10) and immediately homogenized in QIAzol Lysis Reagent (QIAGEN, Valencia, CA), and stored at −80°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5 mL of the culture was immediately treated with a double volume (10 mL) of RNA protect bacteria reagent (Qiagen, Germany) for 5 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... cells were lysed using RLT buffer mastermix (10 uL beta-mercaptoethanol : 1 mL RLT) and cells were lysed with RNAeasy micro kit (Qiagen, 74004). RNA quality and quantity was validated using a nanodrop (ThermoFisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... Genomic DNA was extracted from 10 ♂’s and ♀’s using Blood & Cell Culture DNA Midi Kit (Qiagen, Cat# 13343) and the sequencing library was prepared using the Oxford Nanopore SQK-LSK110 genomic DNA by ligation kit ...
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bioRxiv - Microbiology 2022Quote: ... Soils were sampled at 10 cm depth from the plant’s root and transferred to PowerBead Tubes (QIAGEN DNeasy PowerSoil Kit, Germany) as part of the soil niche ...
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bioRxiv - Genetics 2022Quote: ... Total RNA was extracted from cerebellum of WT and Pum1-/-male and female at 10 weeks of age using the miRNeasy kit (QIAGEN, # 217004) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... after which the cells were FACS sorted for high and low reporter signal.The genomic DNA was extracted by lysing the cell pellets for 10 minutes at 56°C in AL buffer (Qiagen, 19075), supplemented with Proteinase K (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: RNA was extracted from HCC cells cultured in DMEM + 10% FS for 24 hours using a Qiagen RNeasy Plus Mini Kit (Qiagen #74134) with an additional on column DNA digestion using Qiagen TURBO DNase (Qiagen #AM2238 ...
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bioRxiv - Immunology 2022Quote: ... DNA synthesis reaction was carried out in 100 μl using 10 pmol of each primer with HotStarTaq Plus DNA Polymerase (Qiagen, 203603). Purified dsDNA products were amplified with 10 pmol of each primer in a 100 μl total reaction volume ...
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bioRxiv - Neuroscience 2022Quote: Total RNA for each sample was extracted pooled 10 BPs using the RNeasy Plus Micro kit (catalog # 74004, QIAGEN, Venlo, Netherlands) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: We extracted DNA by adding 3-5 mg of tail tissue to 150 µl of 10% ChelexR resin (Bio- Rad, Inc.) with 1.4 µl of Proteinase K (20 mg/ml, Qiagen, Chatsworth, CA), incubating at 55°C for 180 min ...
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bioRxiv - Developmental Biology 2024Quote: ... 10 ng of total RNA was used for first strand cDNA synthesis using miRCURY LNA RT Kit (QIAGEN, Cat. No. 339340). Quantification of the relative miRNAs’ expression level was detected by specific miRCURY LNA miRNA PCR Assays and highly sensitive miRCURY LNA SYBR® Green PCR Kit while adjusting the reverse transcriptase product dilution to 1:30 (QIAGEN ...
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bioRxiv - Molecular Biology 2024Quote: ... or a combination of 25 nM of miR-125b-5p mimic and 10 nM of miR-214 mimic (Qiagen, Hilden, Germany) using lipofectamine 3000 (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... DNA from Gsdmd/D1/D1l3−/− mouse tails was amplified using a genotyping mix containing a dNTP mix (10 mM, Qiagen, 201901), Taq DNA polymerase (250 U ...
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bioRxiv - Developmental Biology 2024Quote: ... 8–10 larvae per genotype were pooled and total RNA was isolated using the Qiagen RNeasy mini kit (Qiagen catalog # 74104) or Zymo Direct-zol RNA Microprep Kit (Zymo Research catalog # R2060) ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-time PCR was performed with 1:10 diluted cDNA using the SYBR green detection system (Thermo Fischer Scientific, F415L) and Rotorgene Q (Qiagen, 9001560) using the 18S rRNA gene as the internal control ...
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bioRxiv - Genetics 2024Quote: For qBiCo-v1 the PCR reaction was optimized in a final volume of 20 µl containing: 10 µl of 2x EpiTect® MethyLight qPCR reagent (Qiagen), 2 μl of 25 mM MgCl2 (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Plasmid DNA was purified from 10% of harvested cells per flask using a QIAprep Spin Miniprep Kit (Qiagen cat. no. 27104). Total RNA was extracted from 90% of harvested cells per flask using a Qiagen RNeasy Midi Kit (Qiagen cat ...
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bioRxiv - Biochemistry 2020Quote: ... (2010) using commercial antibodies directed against the His-tag following the instructions of the manufacture (Qiagen, Hilden, Germany).
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bioRxiv - Cell Biology 2021Quote: Antibodies and their conditions of use are as follows: mouse anti-His (Western blot, 1:1000; 34660; Qiagen), rabbit anti-Brl1 (Western blot ...
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bioRxiv - Neuroscience 2021Quote: RNA was isolated from N2a cells upon antibody treatment for 18 h using the RNeasy Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... antibodies for 24 hrs before 2000 live cells were FACS sorted directly into lysis buffer (RLT buffer (Qiagen)) ...
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bioRxiv - Cancer Biology 2021Quote: ... His-6X-tagged proteins (30 μg/10 μl beads) in 400 μl of PBS were incubated with Ni-NTA Magnetic Agarose Beads (QIAGEN, Hilden, Germany) at room temperature (RT ...
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bioRxiv - Plant Biology 2021Quote: ... Lyophilized material was pulverized in a 2ml screw top microcentrifuge tube with a glass bead for 10 minutes at a frequency of 20/s in a TissueLyser (Retsch ballmill, Qiagen, Venlo, Netherlands). The material was AIR prepped and destarched using protocols from Barnes and Anderson (Barnes and Anderson ...
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bioRxiv - Bioengineering 2020Quote: ... The RBD wildtype and mutant proteins of SARS-CoV-2 spike with 10×his tag at N terminal were expressed in HEK 293 and purified with Ni-NTA affinity columns (Qiagen, Hilden, Germany).
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bioRxiv - Pharmacology and Toxicology 2021Quote: RNA was extracted from clarified cell culture supernatants (16,000 g x 10 min) and from infected cells using QIAamp Viral RNA Mini Kit and RNeasy Plus mini kit (Qiagen, Hilden, Germany), respectively ...
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bioRxiv - Plant Biology 2020Quote: ... the root and leaf dry matter were ground into powder by placing the samples into glass vials with three opposing blades and shaking at a frequency of 30 Hz for 10 minutes using a Qiagen TissueLyser II (Qiagen, ML, USA). Ground root and leaf percent N was determined by the Dumas method using the Elementar rapid N exceed analyzer (Elementar Americas Inc ...
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bioRxiv - Plant Biology 2022Quote: ... DNA was resuspended in 30 μL of mQ water and incubated with 1 μL of RNase (10 mg/mL prepared from a stock of 100 mg/mL of RNase A from Qiagen, Darmstadt, Germany) at 37 °C for 1 h ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA synthesis of 100 ng total RNA was performed with miRCURY LNA RT Kit (10 µl volume reaction) which adds a 5’ universal tag of a poly(A) tail to mature miRNA templates (QIAGEN, Germantown, MD). cDNA template was diluted 1:10 ...
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bioRxiv - Genetics 2022Quote: ... The PCR amplification was carried out in a 10-μl reaction volume containing 8 μl of Taq PCR Master Mix (Qiagen, Hilden, Germany), 10 μM of each primer (1 μl) ...
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bioRxiv - Microbiology 2020Quote: ... 100 μL 0.1mm glass beads were added into the filter casing and put through two 10-minutes cycles of bead beating in a Tissuelyser (Qiagen, Hilden, Germany). The flow-through ...
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bioRxiv - Neuroscience 2021Quote: ... spun down (400 g × 10’ at 4 °C) and the pellet was resuspended in 600μ of Qiazol Lysis Reagent (Qiagen, Cat.No. 79306) and kept at −80 °C before sequencing.