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Citations for Qiagen :
751 - 800 of 5699 citations for Neural Precursor Cell Expressed Developmentally Down Regulated 9 NEDD9 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Cells were then lysed in RLT buffer (QIAGEN) plus 1% ꞵ-mercaptoethanol and homogenized using a QIAshredder column (QIAGEN) ...
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bioRxiv - Molecular Biology 2023Quote: ... hybridoma cells were homogenized using QIAshredder (Qiagen, 79654), and whole RNA was extracted using the Qiagen RNeasy Plus mini kit (Qiagen ...
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bioRxiv - Immunology 2023Quote: Sorted cells were lysed with Qiashredder columns (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Cells were centrifuged and homogenized using QIAshredder (Qiagen). RNA was isolated with RNeasy Micro Kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed in RLT buffer (Qiagen, UK) and extraction was performed using RNeasy mini kit (Qiagen ...
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bioRxiv - Genetics 2023Quote: ... S2R+ cells were transfected using Effectene (Qiagen, 301427) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... cells were taken up in Buffer RLT (Qiagen) and stored at −20 °C ...
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bioRxiv - Genomics 2023Quote: ... and resuspended in Qiagen Cell Lysis Solution (Qiagen).
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bioRxiv - Developmental Biology 2023Quote: ... Additional sequencing performed on gDNA extracted by QIAGEN Blood & Cell Culture DNA Maxi Kit (QIAGEN, 13362), followed by PCR and insertion into Zero Blunt TOPO PCR Cloning Plasmid (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Cells were sorted in RTL Buffer Plus (Qiagen) containing 1% ß-mercaptoethanol (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... miRCURY Exosome Cell/Urine/CSF Kit (Qiagen, 76743) was used to isolate EVs from conditioned medium according to the manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed using QIAzol lysis kit (Qiagen) and RNA was extracted using RNAeasy mini kit (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... brucei cells using the RNeasy mini kit (Qiagen). RNA was treated with Precision DNase Kits (Primer Design ...
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bioRxiv - Microbiology 2023Quote: Cells were sorted into TCL lysis buffer (Qiagen) before extraction of RNA using RNAClean XP beads (Beckman) ...
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bioRxiv - Immunology 2024Quote: ... These cells were homogenized using QIAshredder columns (Qiagen), and RNA was extracted using the RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were collected in RLT Plus Buffer (Qiagen RNeasy Plus Micro Kit ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was extracted from cells by Qiagen RNA extraction kit ...
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bioRxiv - Biochemistry 2024Quote: ... coli cell lysate using Ni-NTA resin (Qiagen). The eluted fusion protein was incubated with Ulp1 protease overnight to remove the His6-SUMO tag ...
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bioRxiv - Biochemistry 2020Quote: ... The primary antibodies were used at the following dilutions: 1:1000 anti-penta-His mouse monoclonal (Qiagen), 1:5000 anti-cMyc mouse monoclonal (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-His Western blots were performed using Penta-His mouse monoclonal IgG1 as primary antibody (Qiagen, #34660) at 1:2000 dilution ...
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bioRxiv - Microbiology 2019Quote: ... and Western blot analysis was performed as previously described (30) using a primary anti-his antibody (Qiagen) and a secondary Alexa Fluor 700 goat anti-mouse antibody ...
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bioRxiv - Microbiology 2021Quote: ... plasmids bearing chimeric antibody heavy and light chain genes were prepared (EndoFree Plasmid Mega prep Kit, QIAGEN) and transfected into Human Embryonic Kidney cells (HEK293F ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein bands were transferred to a nitrocellulose membrane and probed with primary antibodies [mouse anti-His (Qiagen) to detect CAT ...
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bioRxiv - Immunology 2023Quote: ... Plasmids with the cloning of synthesized antibody genes were prepared using the Megaprep plasmid plus kit (Qiagen) for transient transfection ...
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bioRxiv - Microbiology 2023Quote: ... Proteins transferred to a nitrocellulose membrane were probed with a monoclonal anti-His6 antibody (Qiagen, Germantown, Maryland). Detection of proteins via Western blotting was performed by fluorescence detection using IR-Dye®-labeled fluorescent secondary antibodies and imaged using the Odyssey CLx Imager (LICOR Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... The purified recombinant proteins were analyzed by western-blot with anti-His antibody (1:2000 dilution) (Qiagen) followed by HRP-labeled anti-mouse IgG (1:50000 dilution ...
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bioRxiv - Microbiology 2024Quote: ... His-tagged proteins were detected with a mouse anti-His HRP-conjugated antibody (1:3000) from Qiagen. MBP fusion proteins were detected with a rabbit anti-MBP from NEB company 1:1000 followed by an incubation with a goat anti-rabbit HRP-conjugated (1:3000) ...
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bioRxiv - Cell Biology 2019Quote: Genomic DNA from 60 million unsorted cells (500× coverage of the GeCKO v2 library) and 6 million sorted cells was isolated using Blood & Cell Culture DNA Midi Kit (QIAGEN, 13343) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... total RNA was extracted from cells from stable-cell lines created using a RNeasy Plus Mini Kit (Qiagen, catalog number: 74134) following the Manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell pellets were then rinsed with 1X PBS and processed for subcellular fractionation (Qproteome Cell Compartment Kit, Qiagen; following manufacturer’s protocol). Nuclear fractions were isolated and protein precipitated by incubation in 4x volume of ice-cold acetone for 15 minutes followed by pelleting (10 min ...
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bioRxiv - Microbiology 2020Quote: Total cellular DNA was extracted from cells (cell lines or PBMCs) using a QIAmp DNA Blood mini kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA from untreated cells (24 h) and IFNγ-treated cells (6 or 24 h) were extracted with RNeasy plus mini kit (Qiagen, 74134) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... At the end of activation cells were washed twice with PBS and cell pellets were snap-frozen in RLT Plus lysis buffer (Qiagen, #1053393). Total RNA was extracted with the Qiagen’s RNeasy kit according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted for each of the persistently infected cells as well as the cell lines without NDV infection (negative control) using Qiagen RNeasy micro kit (Qiagen, Germany) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Cell lysis and RNA extraction from siRNA transfected cells were performed using the RNeasy RNA extraction kit (Qiagen Inc, Germantown, MD). 1 µg of total RNA was reverse transcribed with the High-capacity RNA-to-cDNA kit (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: E3 and E4 primary microglia were plated at 5×106 cells/well in 6-well plates and RNA was extracted from the cells using the RNEasy Plus Mini Kit (Qiagen #74136) and converted to cDNA using High-Capacity RNA-to-cDNA kit (Thermo #4387406 ...
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bioRxiv - Genomics 2019Quote: ... and the remaining cells in single-cell capture (T1, T2, and T3 samples) using the QIAamp DNA Mini kit (Qiagen, 51304), and 500 ng of DNA were used to construct exome sequencing libraries with the same reagents as the single cell DNA-seq.
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bioRxiv - Genomics 2021Quote: ... the cells were collected and total RNA was extracted from the cells using the Qiagen RNeasy® Plus Kit (Qiagen, 74134). One µg of RNA was then used to synthesize cDNA using the Bio-RAD iScript cDNA Synthesis Kit (Bio-RAD ...
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bioRxiv - Cell Biology 2019Quote: ... MCMBP depleted cells using siRNA and MCMBP-KO (clone #1 and #2) cells was isolated using RNeasy Mini Kit (Qiagen, 74104). The cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kit (Thermo Fischer Scientific ...
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bioRxiv - Genetics 2021Quote: ... S2 cells were seeded at 1 × 106 cells/ml in a 6-well plate and transfected using Effectene (Qiagen, Germantown, MD). For mobility shift assay (Figure 4) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... A pellet of 200-400 µl of animals was resuspended in 400 µl Cell Lysis Solution (Qiagen Gentra Puregen Cell kit) with 5 µl proteinase K (20 mg/ml ...
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bioRxiv - Evolutionary Biology 2022Quote: ... log-phase cells in the range of 1–2 × 108 cells using the Qiagen DNeasy® Blood and Tissue kit (Qiagen). We resuspended the genomic DNA in 10 mM Tris-HCl pH 8.5 and stored it at 4° until submission to the McDonnell Genome Institute at Washington University in St ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from CD33+ ITGA7-sorted primary AML cells (> 4x105 cells/sample) using the RNeasy Mini kit (Qiagen, 74106) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNA from 1,000 H3K27me3 HI/LO β-cells or from 50,000 sorted βHI and βLO cells was extracted using the miRNeasy FFPE Kit (QIAGEN, 217504), followed by the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (E6420L) ...
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bioRxiv - Neuroscience 2024Quote: ... S2 cells were seeded at 1 × 106 cells/ml in a 6-well plate and transfected using Effectene (Qiagen, Germantown, MD). For coimmunoprecipitation (coIP ...
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bioRxiv - Immunology 2024Quote: RNA was isolated from fluorescence-activated cell sorting (FACS) isolated populations of splenic B cells using the Qiagen RNeasy Micro Kit (Qiagen 74004). Library preparation of RNA samples was performed using the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara 634485) ...
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bioRxiv - Molecular Biology 2024Quote: HBEC cells (5e5) from different treatments were mixed mouse RPE1 cells (5e5) for harvesting total RNA using RNeasy kits (Qiagen #74104) for reverse transcription with iScriptTM reverse transcription supermix (Bio-Rad #1708840 ...
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bioRxiv - Molecular Biology 2024Quote: ... ∼5×107 live cells were harvested per replicate and cell pellets underwent same-day nuclei preparation using Qiagen Blood & Cell Culture DNA Maxi Kit (Qiagen, #13362) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... RNAlater was removed from the sorted cells prior to RNA isolation and total RNA was isolated from the pelleted cells using the RNeasy mini kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions with some modifications ...
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bioRxiv - Microbiology 2020Quote: ... RNA from cell lysates or cell culture supernatants was extracted using the Qiagen QIAamp viral RNA mini kit (Qiagen, Valencia, CA, USA). Reverse transcription (RT ...