Labshake search
Citations for Qiagen :
751 - 800 of 1746 citations for Heme oxygenase 1 HO 1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2023Quote: Small cfRNAs were extracted from ~1 mL of plasma using the miRNeasy Serum/Plasma Kit (Qiagen). 1ul ExiSEQ NGS Spike-in (Qiagen ...
-
bioRxiv - Neuroscience 2023Quote: ... RNAs were converted to cDNA using 1 μg RNA and a QuantiTect Reverse Transcription kit (Qiagen) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... Reverse Transcription was performed on 1 µg of RNA using a QuantiTect Reverse Transcription Kit (Qiagen), and qPCR was performed using a QuantiNova SYBR Green PCR kit (Qiagen) ...
-
Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... Cell pellets were resuspended in 1 mL buffer RLT from the RNeasy mini kit (Qiagen, Germany) with a 1:200 dilution of β-mercaptoethanol (Sigma Aldrich ...
-
bioRxiv - Microbiology 2023Quote: ... The lysates were then incubated for 1 h with nickel-nitriloacetic acid (Ni-NTA) beads (Qiagen) at the indicated temperatures (4°C or room temperature) ...
-
bioRxiv - Microbiology 2022Quote: ... PCR products were then treated with DpnI for 1-2 hr and then purified (QiaQuick, Qiagen) before use in library construction.
-
bioRxiv - Microbiology 2023Quote: Total DNA was extracted from frozen cell pellets of 0.3-1×106 PBMCs (Qiagen, Germantown, MD), and concentrated using an ethanol precipitation protocol with GlycoBlue following manufacturer’s instructions (Thermofisher ...
-
bioRxiv - Biochemistry 2023Quote: ... The cell lysate was clarified by centrifugation and incubated with 1 mL Ni-NTA resin (Qiagen). The precursor protein was purified using the standard protocol from Qiagen for purifying protein from Ni-NTA resin under denaturing conditions with urea ...
-
bioRxiv - Plant Biology 2023Quote: ... 1 µg of RNA was reverse transcribed into cDNA using the QuantiTect Reverse Transcription Kit (Qiagen). Transcript quantification was performed in 96-well plates ...
-
bioRxiv - Genomics 2023Quote: ... with each 20 μL reaction consisting of 1× QuantiFAST reaction mix containing ROX reference dye (Qiagen), 0.66 µM forward and reverse primers ...
-
bioRxiv - Plant Biology 2023Quote: ... 1 µg of RNA was reverse transcribed into cDNA using the QuantiTect Reverse Transcription Kit (Qiagen). Transcript quantification was performed in 96-well plates ...
-
bioRxiv - Genomics 2023Quote: ... for 1 hour at room temperature followed by isolation with a minElute RNA Cleanup Kit (Qiagen). RNA concentrations were measured by Nanodrop and total RNA integrity assayed using an Agilent Bioanalyzer ...
-
bioRxiv - Microbiology 2023Quote: ... each spot was collected into a 1:2 mix of LBS and RNAprotect Bacteria reagent (Qiagen), incubated at RT for 5 minutes ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 µg of total mRNA from each sample was retrotranscribed using QuantiTech reverse Transcription Kit (Qiagen). Real-Time PCR was performed using SYBR Green Master Mix (Bio-Rad ...
-
bioRxiv - Biochemistry 2023Quote: ... The lysates were loaded onto a 1 mL Ni-NTA Superflow column (Qiagen, Valencia, CA, USA) that had been equilibrated with 10 column volumes of wash 1 buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... Recombinant proteins were purified on Ni-NTA columns (1 ml Ni-NTA Agarose; Qiagen Cat # 30210), washed with 20 ml hexokinase buffer (20 mM HEPES ...
-
bioRxiv - Systems Biology 2023Quote: ... About 1 OD/mL cells were mixed with 2 volumes of RNAprotect Bacteria Reagent (Qiagen, 76506), and the cell pellet was collected by centrifuging at 2500 g for 8 minutes at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... Bacteria (∼1 × 109 cells) were removed from the cultures and suspended in RNAprotect bacteria reagent (Qiagen), pelleted by centrifugation ...
-
bioRxiv - Microbiology 2023Quote: ... genomic DNA was extracted from 1 ml of overnight culture (DNeasy Blood and Tissue Kit—Qiagen) pre-treated with 100 μg of lysostaphin (Sigma cat ...
-
bioRxiv - Microbiology 2023Quote: ... These were harvested by centrifugation and resuspended in 1 mL of RNA Protect Bacteria Reagent (Qiagen) with 30µL of 20 mg/mL lysozyme ...
-
bioRxiv - Genomics 2023Quote: ... cfDNA was isolated from 1 mL of plasma using the QIAGEN Circulating Nucleic Acids Kit (QIAGEN), eluted in AE buffer ...
-
bioRxiv - Physiology 2023Quote: ... PCR was performed using 1 μl of cDNA and Top Taq DNA polymerase and buffer (Qiagen). The reactions were performed for 30 s at 94 °C ...
-
Vps18 contributes to phagosome membrane integrity in Mycobacterium tuberculosis-infected macrophagesbioRxiv - Microbiology 2023Quote: Bulk or flow-sorted THP-1 macrophages were lysed with β-ME-supplemented Buffer RLT (Qiagen). RNA was isolated from the lysate using an RNEasy plus kit (Qiagen ...
-
bioRxiv - Molecular Biology 2024Quote: ... castellanii cells were transfected with 1 μg of pUbg-R252 plasmid using Effectene transfection reagent (QIAGEN) in a 60 mm dish following the manufacturer’s protocol ...
-
bioRxiv - Genomics 2024Quote: ... 1 mL of PE buffer and 100 µL of EB buffer (MinElute PCR Purification Kit, QIAGEN). The silica columns were placed in a collection tube to dry and later in a 1.5 mL DNA lo-bind tube (Eppendorf ...
-
bioRxiv - Immunology 2024Quote: ... 1 mL of PBS-AB was added to each tissue with Tungsten carbide 3mm beads (Qiagen). Samples were homogenized for 15 min and then centrifuged at 15,000 g for 10 min at 4°C ...
-
bioRxiv - Bioengineering 2021Quote: Genomic DNA from human primary CD4+/CD8+ T cells was isolated using the Gentra Puregene Kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... frozen fragments of human cortex were homogenized with a motorized hand pestle in RLT lysis buffer (Qiagen) and qPCR was performed as described in qPCR section.
-
bioRxiv - Microbiology 2021Quote: Genomic DNA (gDNA) from human blood cells was isolated using the QIAamp Blood kit (QIAGEN, Hilden, Germany). To isolate gDNA from different mouse tissues we made use of the blackPREP Rodent Tail DNA Kit (Analytik Jena ...
-
bioRxiv - Cancer Biology 2020Quote: Total genomic DNAs (containing human and viral genomes) were isolated using AllPrep DNA/RNA Mini Kit (Qiagen) from the NPC biopsy ...
-
bioRxiv - Cancer Biology 2020Quote: DNA from human and murine PC cells were isolated using the DNeasy blood and tissue kit (Qiagen) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2019Quote: ... The RT2 Profiler “Inflammatory cytokines and receptors” and “Human innate and adaptive response” arrays PCR Arrays (Qiagen) were performed according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: ... Genomic human DNA was isolated from whole blood using QIAamp DNA Blood Mini Kit from Qiagen (51106) and phosphodiester DNA from TIB Molbiol ...
-
bioRxiv - Paleontology 2019Quote: Human 2 and 3: DNA was extracted from bones using QIAamp® DNA Investigator kit (56504, Qiagen). Bones were thoroughly washed (X5 ...
-
bioRxiv - Microbiology 2020Quote: ... Two mouse and two human stool samples were homogenized using a PowerLyzer 24 Homogenizer (110/220V; Qiagen). DNA was extracted using four different bead beating times ...
-
bioRxiv - Genetics 2019Quote: RNA was extracted from PCYT1A-wild-type human fibroblasts using the RNeasy Mini Kit (Qiagen, Cat#74104), reverse-transcribed according to the manufacturer’s protocol (SuperScriptIII One-Step RT-PCR system with Platinum Taq DNA Polymerase;Invitrogen ...
-
bioRxiv - Cell Biology 2022Quote: ... Human ATGL-targeting and cPLA2α-targeting siRNAs and the AllStars Negative Control siRNA were from Qiagen (Germany). T863 (DGAT1 inhibitor) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Reads from *.fastq files were mapped to the rat reference genome (Rattus norvegicus reference genome Rnor_6.0) or the human reference genome (Homo sapiens reference genome GRCh37) using CLC Genomics Workbench 20.0.4 (Qiagen). Only reads with <2 mismatches and minimum length and a similarity fraction of 0.8 were mapped to the reference genome ...
-
bioRxiv - Cell Biology 2023Quote: Total RNA of human macrophages was isolated according to manufacturer’s instructions using the RNeasy extraction kit (Qiagen). mRNA was extracted using the Next Poly(A ...
-
bioRxiv - Cell Biology 2023Quote: Uninfected or infected primary human macrophages were subjected to RNA extraction using the RNeasy extraction kit (Qiagen). Two µg of RNA each was reverse transcribed with the iScript cDNA Synthesis Kit (Bio-Rad) ...
-
bioRxiv - Immunology 2022Quote: Genomic DNA was purified from human peripheral blood mononuclear cells (PBMCs) using QIAamp kits (Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... and the pellet was lysed using RLT buffer (mouse cells) or QIAZol (human cells) (Qiagen, Cat. 79306). RNA was extracted using the Qiagen RNeasy micro kit.
-
bioRxiv - Molecular Biology 2024Quote: ... Total RNA was extracted from human tissue and cells using the miRNeasy Mini kit (Qiagen, Hilden, Germany) or TRIzol reagent (Thermo Fisher Scientific).
-
bioRxiv - Genetics 2024Quote: The RNA quality of human kidney FFPE sample was checked by RNeasy FFPE kit (Qiagen-Cat #73504) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: RNA from 1205Lu cells and immortalized human melanocytes were isolated and purified using RNeasy Micro Kit (Qiagen) according to manufacturer’s instruction and concentration was quantified using a NanoDrop Spectrophotometer (ThermoScientific) ...
-
bioRxiv - Genomics 2024Quote: ... the effect of human rRNA removal was also assessed using a QIAseq FastSelect rRNA removal kit (Qiagen). The rRNA removal reaction was performed after RNA thermal denaturation at 95℃ 5 min according to the instructions.
-
bioRxiv - Developmental Biology 2021Quote: ... Genomic DNA (gDNA) was extracted from 1-5×106 cells using DNeasy Blood and Tissue Kit (Qiagen) and 250 ng gDNA ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... DNA was recovered using a chloroform-ethanol procedure (Supplementary protocol 1) and diluted in AE buffer (Qiagen). A pair-ended library was constructed by Eureka Genomics (Hercules ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was extracted from 1 ml of overnight culture using a QIAamp DNA Mini Kit (Qiagen). Illumina MiSeq sequencing operated by the Functional Genomics Centre Zurich and Novogene (Cambridge ...
-
bioRxiv - Biochemistry 2020Quote: ... The primary antibodies were used at the following dilutions: 1:1000 anti-penta-His mouse monoclonal (Qiagen), 1:5000 anti-cMyc mouse monoclonal (Sigma) ...