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Citations for Qiagen :
751 - 800 of 2270 citations for 2 Chloro 4 3 fluoro 4 hydroxyphenyl benzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Amplification of cDNA for validation of doxycycline induction was performed with U2AF1 primers (forward: 5’-GGCACCGAGAAAGACAAAGT-3’; reverse: 5’-CTCTGGAAATGGGCTTCAAA-3’) and PCR products were purified using QIAquick PCR purification kit (QIAGEN, Cat #28104) before Sanger sequencing ...
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bioRxiv - Microbiology 2022Quote: ... A wild-type virus stock NL4-3 was prepared by transfection of the pNL4-3 plasmid (purified using the Qiagen Plasmid Maxikit) into HeLa cells ...
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bioRxiv - Plant Biology 2020Quote: Reduced His-tagged PRX-IIE (3 mg) or PRX-IIE C146S (3 mg) were bound to 1 mL Ni-NTA resin (Qiagen, Hilden, Germany) and used as affinity matrix ...
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bioRxiv - Microbiology 2020Quote: ... region was amplified using fungal-specific primers (60): ITS1F (5’-CTTGGTCATTTAGAGGAAGTAA-3’) and ITS4 (5’-TCCTCCGCTTATTGATATGC-3’) and the HotStarTaq Plus Master Kit (Qiagen, Valencia, CA). Amplicons from different samples were pooled to equimolar concentrations and purified of short fragments using Agencourt Ampure beads (Agencourt Bioscience Corporation ...
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bioRxiv - Genomics 2021Quote: ... Total RNA was isolated from the VTM in a biosafety level-3 (BSL-3) laboratory using QIAamp viral RNA mini kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... of the 16S rRNA gene that amplified via PCR using primers 338F (Seq: 5’-ACTYCTACGGRAGGCWGC-3’) and 1061R (Seq: 5’-CRRCACGAGCTGACGAC-3’) with the HotStar HiFidelity Polymerase Kit (catalog number 202602; QIAGEN, Hilden, Germany). Supplementary Figure 1 shows the 16S rRNA gene map and the primers used ...
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bioRxiv - Neuroscience 2022Quote: ... and 18S rRNA (5’-GAG CGA AAG CAT TTG CCA AG-3’ and 5’-GGC ATC GTT TAT GGT CGG AA-3’) on a Roter-Gene Q (Qiagen, Hilden, Germany) with 2x AmpiGene® qPCR Green Mix Lo-ROX (Enzo Biochem ...
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bioRxiv - Microbiology 2024Quote: ... and homogenized in groups of 3-5 flies by bead-beating with 3 x 5 mm stainless steel beads (Qiagen, Hilden, Germany) in 1 ml 1X PBS ...
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bioRxiv - Plant Biology 2022Quote: ... using 3-mm steel beads (Cat No.: 69997, Qiagen); tubes were shaken for 20-s at 28 Hz with dry ice.
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bioRxiv - Evolutionary Biology 2022Quote: ... with two sterile 3 mm beads (Qiagen, Hilden, Germany) and a bead device at 20 Hz for 3 min ...
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bioRxiv - Immunology 2020Quote: ... and prepared the QIAseq UPX 3’ Transcriptome Kit (QIAGEN). 10ng purified RNA was used for the NGS libraries ...
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bioRxiv - Microbiology 2020Quote: ... cells were lysed in 3 volumes RLT buffer (Qiagen) and RNA was extracted using Dynabeads MyOne Silane (Life technologies) ...
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bioRxiv - Immunology 2021Quote: 1-3 × 105 PBMCs were lysed in QIAzol (Qiagen). Full-length cDNA was then synthesized using the SMARTer technology (Takara Bio) ...
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bioRxiv - Biochemistry 2021Quote: ... using a TissueLyser II (3 min, 30 Hz; Qiagen). After vigorous mixing with chloroform at a 1:4 v/v ratio (chloroform:TRIzol) ...
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bioRxiv - Genomics 2021Quote: ... 3 μl of nuclease free water (Qiagen, Hilden, Germany) and 5 μl of template containing cDNA ...
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bioRxiv - Molecular Biology 2023Quote: ... for 3 min in TissueLyser II (30 Hz; Qiagen). Cell lysates were centrifuged for 15 min (4 °C ...
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bioRxiv - Immunology 2023Quote: ... using tungsten carbide beads (3 mm, Qiagen catalog #69997) and shaking (300 times per min ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 volumes of QIAzol® (Qiagen, Cat. No. 79306) were added to 80 µl of cell extracts ...
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bioRxiv - Cell Biology 2022Quote: ... and oligo 5 (Q4) 5’-CACCGAGCTCTTCGCCGAGTA-3’ (Qiagen, SI03058272).
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bioRxiv - Physiology 2023Quote: ... using a TissueLyser II (3 min, 30 Hz; Qiagen). Lysates were centrifuged for 15 min (21,000 g ...
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bioRxiv - Developmental Biology 2020Quote: ... and the soluble homogenates were purified by Ni-nitrilotriacetic acid (NI-NTA) Agarose (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... nucleic acids were extracted from frozen faecal aliquots using the RNeasy PowerMicrobiome kit (Qiagen). The manufacturer’s protocol was modified by the addition of a heating step at 90°C for 10min after vortexing and by the exclusion of DNA-removal steps ...
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bioRxiv - Genomics 2020Quote: ... purified nucleic acids were treated with 1-5 μl 1 mg/ml RNaseA (Qiagen) for 45 minutes at 37 °C to degrade RNA ...
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bioRxiv - Systems Biology 2021Quote: ... The supernatant was loaded on a nickel nitrilotriacetic acid (Ni-NTA) resin (Qiagen GmbH) column ...
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bioRxiv - Cell Biology 2021Quote: ... We purified the 6xHIS fusion protein on nickel-nitrilotriacetic acid agarose (Qiagen, Valencia, CA) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... Nucleic acids were extracted with the Qiagen Power Fecal Pro kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
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Heterologous expression of Dehalobacter spp. respiratory reductive dehalogenases in Escherichia colibioRxiv - Microbiology 2021Quote: ... and nickel-nitrilotriacetic acid (Ni-NTA) agarose resin was purchased from Qiagen (Hilden, Germany). PCR reagents ...
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bioRxiv - Cancer Biology 2020Quote: ... Nucleic acids were subsequently extracted using the AllPrep DNA/RNA Mini Kit (QIAGEN, #80204) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... The enzyme was purified through a nickel-nitrilotriacetic acid column (Ni-NTA superflow, Qiagen), which was followed by a gel filtration column (Superdex 200 ...
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bioRxiv - Developmental Biology 2022Quote: ... and 1.2 µM custom template-switching oligo with a Locked Nucleic Acid analog (Qiagen) at 42°C for 90 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Amino acid locations were confirmed through sequence alignment using MacVector and CLC Workbench (QIAGEN).
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bioRxiv - Immunology 2020Quote: ... The His-tagged fusion peptide was purified using nickel–nitrilotriacetic acid (Ni–NTA, Qiagen) resin affinity chromatography and by C18 reverse-phase chromatography (Sep-Pak® Waters ...
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bioRxiv - Microbiology 2022Quote: Total nucleic acids were extracted in duplicate using the PowerSoil-Kit (MO-BIO, Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... S1 RBD was purified from the culture supernatant by nickel–nitrilotriacetic acid agarose (Qiagen), and purity was confirmed to by >95% as judged by coomassie stained SDS-PAGE ...
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bioRxiv - Plant Biology 2020Quote: ... His-tagged proteins were incubated with Nickel-nitrilotriacetic acid (NTA) sepharose (Qiagen, Hilden, Germany) at 4°C with slight shaking for 1 h ...
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bioRxiv - Plant Biology 2020Quote: ... coli Rosetta cells was carried out using nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen) as described in Barja and Rodríguez-Concepción (2020) ...
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bioRxiv - Molecular Biology 2021Quote: ... we added 70 µl of Ni-NTA (Nickel Nitrilo-triacetic Acid) agarose beads (Qiagen), and incubated the mixture overnight at the orbital rotator at RT ...
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bioRxiv - Microbiology 2020Quote: ... 0.5 ml of culture supernatant was incubated with nickel-nitrilotriacetic acid-agarose beads (QIAGEN). The beads were washed ...
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bioRxiv - Molecular Biology 2023Quote: ... After purification of AtH2B.9 using nickel-nitrilotriacetic acid agarose (Ni-NTA) resin (QIAGEN), the His6-tag portion was removed by TEV protease (30 µg/mg of His6-H2B.9) ...
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bioRxiv - Microbiology 2023Quote: Nucleic acid extraction was carried out using DNeasy PowerSoil Pro Kit (QIAGEN, Hilden, Germnay) following manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... Proteins were purified from filtered supernatants using nickel nitriloacetic acid (Ni-NTA) agarose (QIAGEN) via gravity flow ...
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bioRxiv - Developmental Biology 2023Quote: ... specific locked nucleic acid (LNA) probes doubly-labelled with digoxigenin were purchased from Qiagen and ISH was conducted following the published protocol (Chen et al. ...
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Safe plant Hsp90 adjuvants elicit an effective immune response against SARS-CoV2 derived RBD antigenbioRxiv - Molecular Biology 2023Quote: ... Soluble RBD was purified under denaturing conditions using a nitrilotriacetic acid-Ni2+ column (Qiagen)[19] ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatant was loaded onto a nickel-nitriloacetic acid (Ni2+-NTA) column (Qiagen, Hilden, Germany), washed with 500ml buffer A ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was applied to a 5 ml nickel-nitrilotriacetic acid column (Qiagen, Valencia, CA) that had been equilibrated with buffer A at a rate of 1 ml/min ...
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bioRxiv - Biophysics 2022Quote: The obtained supernatant was incubated with Ni-nitrilotriacetic acid (Ni-NTA) resin (Qiagen, Germany) in a rotating shaker for 1 h ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The clarified lysate was incubated with nickel-nitrilotriacetic acid (Ni-NTA) agarose resin (Qiagen) for 1 hour with agitation ...
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bioRxiv - Synthetic Biology 2023Quote: ... the clarified lysate was loaded on a Ni-nitrilotriacetic acid (NTA) agarose column (Qiagen) using the ÄKTA Explorer fast protein liquid chromatography system (FPLC ...
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bioRxiv - Immunology 2024Quote: ... Total RNA is isolated using the column based nucleic acid purification kit (RNeasy, Qiagen), strictly according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were subjected to pull down using nickel-nitrilotriacetic acid (Ni-NTA) beads (Qiagen) overnight at 4 °C on a rotation platform ...