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Citations for Qiagen :
751 - 800 of 2556 citations for 2 Amino 6 chloro 9 3 5 di O p toluoyl beta D 2 deoxyribofurnanosyl purine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... and sunflower_KTI _R (5’ – CTACTCAGATTGAACAGAAGCCAC- 3’were designed and used in a PCR reaction with total DNA extracted (DNeasy Plant Mini Kit, Qiagen, Valencia CA USA) from sunflower leaf tissue ...
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bioRxiv - Immunology 2023Quote: Whole livers were harvested at indicated timepoints and placed into a 7 ml homogenizer tube (Omni International Cat. No. 19-651) containing 3 mL sterile PBS and one 5 mm stainless steel bead (QIAGEN Cat. No. 69989). Spleens and single granulomas were harvested at indicated timepoints and placed into a 1 ml homogenizer tube (Fisher Brand Cat ...
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bioRxiv - Physiology 2023Quote: ... Ganglia in 1 mL of TRIzol reagent were homogenized using a TissueLyzer II bead mill (one 5 mm stainless steel bead per tube, 30 s-1 frequency for 3 minutes; Qiagen Inc. Hilden, Germany). The homogenates were incubated with 200 µL chloroform and centrifuged for 15 minutes at 12000 x g to isolate the protein-containing organic phase ...
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bioRxiv - Cell Biology 2021Quote: ... Differentially expressed genes (P<0.01) were analysed with Ingenuity Pathway Analysis software (Qiagen) applying the Upstream Regulator Analysis approach ...
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bioRxiv - Neuroscience 2022Quote: ... The P values for DEGs were then uploaded to Ingenuity Pathway Analysis (Qiagen), which was used to analyze the cellular pathways determined by the differentially expressed genes.
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bioRxiv - Genetics 2021Quote: ... DNA was extracted using Qiagen 96-well DNeasy kits (Qiagen P/N 69581). Sequencing libraries were prepared using the Illumina Nextera Kit and custom barcoded adapter sequences ...
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bioRxiv - Immunology 2022Quote: ... and adjusted P-value were used to input into IPA (version 43605602; QIAGEN). Initial interrogation of each dataset was performed using default values and parameters set on IPA ...
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bioRxiv - Molecular Biology 2023Quote: ... organs from adult mice (9-13 weeks old) were harvested and homogenized in Qiazol lysis reagent (Qiagen) and total RNA was isolated by phenol–chloroform extraction according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... organs from adult mice (9-13 weeks old) were harvested and homogenized in Qiazol lysis reagent (Qiagen) and total RNA was isolated by phenol–chloroform extraction according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The remaining procedure was identical to that provided in the DNeasyPlant Mini protocol (steps 9-19, Qiagen). After DNA quality and quantity filtering ...
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bioRxiv - Microbiology 2024Quote: ... Extraction was performed on the 9 samples using the DNeasy® PowerSoil® Pro Kit (QIAGEN, Germany) according to the manufacturer’s protocol.
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bioRxiv - Biophysics 2021Quote: ... The solubilized fraction was then incubated for 2 h with 300 µL of pre-equilibrated Ni-NTA resin (QIAGEN) in Ni-NTA buffer (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: cfDNA was isolated from 2 mL of plasma for each sample via the QIAamp Circulating Nucleic Acid kit (Qiagen). ddPCR was performed using the QX200 Droplet Digital PCR System (Bio-Rad) ...
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bioRxiv - Developmental Biology 2021Quote: ... the second PCR used 2 µL of the first reaction as template and HotStarTaq DNA Polymerase (Qiagen, Hilden, Germany) in a 20 µL reaction volume for 15 cycles ...
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bioRxiv - Immunology 2021Quote: ... RNA of cells exposed to SARS-CoV-2 WT was isolated with the QIAamp Viral RNA Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... PCR 2 products were purified by electrophoresis with a 1% agarose gel using a QIAquick Gel Extraction Kit (Qiagen) and eluting with 30 μL water ...
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bioRxiv - Evolutionary Biology 2020Quote: DNA was extracted from the exterior (outermost 2 mm) tissue and water filters using the DNeasy PowerSoil Kit (Qiagen) and from the interior tissue (at least 2 mm away from the outermost edge of the specimen ...
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bioRxiv - Neuroscience 2022Quote: RNA was isolated from snap frozen striatal mouse tissue from 2 independent cohorts of mice according to the manufacturer’s protocol and purified using RNeasy columns (Qiagen). A total of 13 mRNA libraries for Illumina sequencing were prepared using KAPA mRNA HyperPrep Kit (KAPA Biosystems ...
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bioRxiv - Immunology 2022Quote: ... in vitro generated PCs were sorted at day 2 and RNA were extracted with “RNeasy plus micro kit” (Qiagen) as recommended ...
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bioRxiv - Genomics 2020Quote: ... and RNA was eluted using DTT (100mM, 2 × 100μL) and collected into 700μL RLT buffer from the RNeasy MinElute Cleanup Kit (Qiagen 74204). RNA was isolated using the RNeasy MinElute kit as per manufacturer’s instructions and was quantified using the Agilent Tapestation High Sensitivity RNA kit (Agilent 067-5576) ...
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bioRxiv - Neuroscience 2020Quote: ... each individual cell was transferred to a 0.2 ml PCR tube containing 2 μl of lysis buffer (RLT Plus - Qiagen). The tube was immediately placed on a metal plate sitting on top of dry ice for flash-freezing ...
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bioRxiv - Biophysics 2021Quote: The SARS-CoV-2 RNA was isolated from virus particles with the QIAmp viral RNA mini kit (Qiagen - USA) and reversely transcribed to cDNA with the High-Capacity Reverse Transcription Kit (Thermo - USA) ...
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bioRxiv - Immunology 2021Quote: ... was then added to a polymerase chain reaction (PCR) mix containing 12.5 μl of 2× Multiplex PCR mix (Qiagen), 9 μl of H2O ...
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bioRxiv - Cell Biology 2021Quote: Meta-analysis of the DESeq2 analysed RNA sequencing data was performed to identify the top pathways that are statistically associated with the list of the significantly induced genes77 (fold change > 2, FDR q value < 0.05, using the Ingenuity Pathway Analysis platform IPA, Ingenuity Systems, Qiagen).
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bioRxiv - Cell Biology 2021Quote: Total RNA extracts were prepared from a minimum of 2×104 freshly sorted cells using RNeasy Micro Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 2 μg of microRNA was reverse transcribed using miScript Ⅱ microRNA cDNA Synthesis Kit (QIAGEN Biotechnology Co, Ltd, China). The reverse transcription reaction system included 4 μL of 5×miScript HiFlex Buffer ...
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bioRxiv - Microbiology 2021Quote: The QIAseq SARS-CoV-2 Primer Panel paired with the QIAseq FX DNA Library construction kits (Qiagen GmbH, Germany) were used for enriching and sequencing the entire SARS-CoV-2 viral genome ...
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bioRxiv - Molecular Biology 2020Quote: ... was added to the embryos and homogenized for 2 min at 40 Hz using a Tissue Lyser LT (Qiagen) and 5 mm stainless steel beads (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from supernatants of SARS-CoV-2-infected Vero E6 cells using the RNeasy kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from 2 g powdered clay samples using the PowerMax Soil DNA Isolation Kit (Qiagen, Germany) and modifications to the manufacturer’s instructions previously validated (22) ...
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bioRxiv - Microbiology 2022Quote: ... 1 mL of the 2-mL culture was removed and replaced by 1 mL of RNAprotect Cell Reagent (Qiagen). After 5 min of incubation ...
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bioRxiv - Microbiology 2022Quote: ... One volume of bacterial culture containing approximately 0.2 OD600 of cells was mixed with 2 volumes of RNAprotect (Qiagen) and processed according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Purified ZIKV RNA and HSV-2 DNA were extracted using QIAamp MinElute Virus Spin Kit (Qiagen, catalog no. 57704), and 50ng purified viral RNA/DNA was incubated with the recombinant protein at different concentrations for 90min at 37°C in the presence of SUPERase In™ RNase inhibitor (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...
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bioRxiv - Biochemistry 2020Quote: ... Nanobody PCR fragments were analyzed on 2% TAE-agarose gels and purified with the QIAquick gel extraction kit (Qiagen). PCR fragments were sequenced and individual nanobodies were cloned using the In-Fusion cloning kit (Takara Bio ...
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bioRxiv - Cancer Biology 2021Quote: ... The products were run on 2% agarose gel and extracted using the QIAquick Gel Extraction Kit (Qiagen, Cat#28706). The purified products were submitted for paired-end 300 bp sequencing on MiSeq (Illumina) ...
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bioRxiv - Developmental Biology 2022Quote: ... the cells were transfected with a mixture of 2 mg SPACA6 pMT-puro vector and Effectene transfection reagent (Qiagen), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... 200 RPM for 2 h then approximately 1×109 CFU of bacteria were mixed at a 2:1 (vol:vol) ratio of RNAProtect (Qiagen) and incubated at room temperature for 5 min ...
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bioRxiv - Genomics 2022Quote: ... PCR#1 amplicons were selected on a 2% agarose gel and purified using the QIAquick Gel Extraction Kit (Qiagen). These amplicons were then used as template for “PCR#2” reactions ...
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bioRxiv - Genomics 2022Quote: ... PCR amplicons were size selected on a 2% agarose gel and purified using the QIAquick Gel Extraction Kit (Qiagen), and sequenced and deconvoluted using the Amplicon-EZ service provided by GENEWIZ/AZENTA (South Plainfield ...
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bioRxiv - Physiology 2022Quote: Frozen liver tissue was manually crushed and 30 mg per sample was homogenized in 1mL of 2:1 chloroform:methanol via a TissueLyser II (Qiagen). Samples were stored at 4 °C overnight with agitation ...
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bioRxiv - Cancer Biology 2020Quote: ... The libraries were isolated from a 2% agarose gel using the QiaQuick gel extraction kit (Qiagen, Cat. No. 28706) and their quality and concentration were determined using a 2200 TapeStation and D1000 screen tapes (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... tissue was digested away from the slide by incubating the tissue with 1% 2-mercaptoethanol in RLT buffer (Qiagen) for one hour at 56°C with interval shaking ...
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bioRxiv - Systems Biology 2021Quote: RNA isolation from SARS-CoV and SARS-CoV-2 infected A549-ACE2 cells was performed as described above (Qiagen). 500 ng total RNA was used for reverse transcription with PrimeScript RT with gDNA eraser (Takara) ...
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bioRxiv - Physiology 2021Quote: ... and homogenized with metal beads (2.4 mm diameter, Askubal) for 2 min at 25 Hz in a TissueLyzerII (Qiagen). Beetles were weighed and frozen in liquid nitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... Total protein was normalized to 2 mg across all samples tested and incubated with 0.25 mL of 50% Ni-NTA resin (Qiagen) for 2 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 g of meat or processed food powder was extracted and purified using DNeasy Mericon Food Kit (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: Libraries were constructed using QIASEQ SARS-CoV-2 Primer Panel and QIAseq FX DNA Library UDI-A kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: Tissue lysates were prepared from 30 pooled 2 dpf WT and MZdnmt3aa-/- mutant by homogenization using TissueLyser LT (QIAGEN) with zirconia beads for 10 s at 50 Hz ...
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bioRxiv - Biochemistry 2020Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...