Labshake search
Citations for Qiagen :
701 - 750 of 1134 citations for Water Nuclease Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted following the protocol of the DNeasy Power water DNA Isolation kit (Qiagen, Venlo, the Netherlands).
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bioRxiv - Microbiology 2023Quote: ... and DNA was eluted with molecular biology grade water (protocol based on personal communication with a Qiagen representative).
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bioRxiv - Neuroscience 2024Quote: ... RNA samples were resuspended in water and further purified with RNeasy columns with on-column DNase treatment (Qiagen). RNA purity was assessed by measuring the A260/A280 ratio with a NanoDrop ...
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bioRxiv - Cell Biology 2020Quote: ... DNase treatment was performed using RNase-Free DNase Set (cat. 79254 - Qiagen, Hilden, Germany). Total RNA was eluted in 11 μl of DNase/RNase-Free Water and LCM captures deriving from human sample were pooled and subjected to a subsequent concentration through Savant SpeedVac SC100 centrifugal evaporator ...
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bioRxiv - Immunology 2021Quote: ... and on-column DNA digestion was performed with RNase-free DNase Set (Qiagen 74106). For cDNA synthesis ...
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bioRxiv - Physiology 2021Quote: ... The RNeasy Mini Kit (74104) and RNase-free DNase set (79254) were from Qiagen. High-Capacity cDNA Reverse Transcription Kit (4368814 ...
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bioRxiv - Developmental Biology 2020Quote: ... along with on-column digestion of DNA with RNase-Free DNase Set (Qiagen, 79254) following the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2019Quote: ... DNase treatment in columns was carried out with RNase-free DNase (Qiagen, Venlo, Netherlands) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... including the optional on column DNase Digestion with the RNAse-Free DNase Set (Qiagen). Total RNA quality was confirmed on the 2100 Bioanalyzer instrument (Agilent ...
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bioRxiv - Neuroscience 2019Quote: ... and subjected on-column incubation with RNase-free DNase according to manufacturer’s protocol (Qiagen). RNA quality and integrity was measured on the 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Physiology 2020Quote: ... DNAse digest was performed on column using the RNase-Free DNase Set (Qiagen 79254) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... Contaminating gDNA was digested by on-column DNase treatment using RNase-free DNase (Qiagen) and total RNA was eluted with 80 μL of nuclease-free water (Qiagen) ...
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bioRxiv - Neuroscience 2020Quote: ... Contaminating DNA was degraded by treatment of the samples with RNase-free DNase (Qiagen) in column ...
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bioRxiv - Cancer Biology 2020Quote: ... A DNAse digestion step was included using the RNAse-free DNAse set (Qiagen #79254). Total RNA was then isolated using the miRNeasy Mini Kit (Qiagen # 74004) ...
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bioRxiv - Biochemistry 2020Quote: ... The cell free supernatant was loaded on to pre-equilibrated Ni2+-nitrilotriacetic resin (Qiagen), and incubated for 1hr at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... An on-column digestion step using the RNAse-free DNAse Set (Qiagen, Cat.No# 79254) was included for all RNA-Seq samples ...
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bioRxiv - Plant Biology 2020Quote: ... DNA digestion was performed using the RNase-Free DNase Set (Cat No.: 79254, Qiagen) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... RNA was treated twice with the Qiagen RNAse-free DNAse set (Qiagen, Hilden, Germany) to remove DNA ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was treated with DNase by column-based purification (RNase-Free DNase Kit, Qiagen). RNA libraries were prepared and sequenced on an Illumina HiSeq 4000 system ...
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bioRxiv - Plant Biology 2021Quote: ... Each mRNA extraction was treated with QIAGEN’s RNase-Free DNase Set (QIAGEN, Hilden, Germany), involving a first round of the TURBO DNA-free™ DNA Removal Kit through the extraction protocol and then two rounds of the DNA-free™ Kit (Life Technologies ...
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bioRxiv - Plant Biology 2020Quote: ... DNase treatment was performed to remove DNA using the RNase-Free DNase Set (Qiagen) eluted in 50 mL of RNase-free water ...
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bioRxiv - Cell Biology 2022Quote: ... including an on-column DNA digestion using the RNAse-free DNAse set (Qiagen; 79254), was used to purify RNA from whole organoids ...
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bioRxiv - Developmental Biology 2022Quote: ... The optional DNase treatment was carried out with the RNase-Free DNase Set (Qiagen). RNA quality was checked and quantified by spectrophotometry (Nanodrop Technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... An on-column DNase digestion was performed with the RNase-free DNase set (Qiagen). RNA was quantified with the Nanodrop 2000c (ThermoFisher) ...
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bioRxiv - Developmental Biology 2022Quote: ... Aliquots of reconstituted DNaseI were prepared from the RNase-free DNase Set (Qiagen, 79254) ahead of time and the appropriate amount of DNaseI in RDD buffer was prepared just prior to starting the extraction ...
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bioRxiv - Microbiology 2022Quote: ... DNA removal during extraction was carried out by using RNase-Free DNase I (Qiagen). RNA concentration and quality were measured using Qubit 1.0 fluorometer (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... with on-column removal of DNA using the RNase-Free DNase Set (Qiagen 79254). The cDNA library was prepared with the KAPA mRNA HyperPrep Kit (Roche 08098115702 ...
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bioRxiv - Plant Biology 2021Quote: ... Contaminating genomic DNA was removed by digestion with RNase-Free DNase Set (QIAGEN, Germany). Library preparation and data analysis were performed as described previously (Shih et al. ...
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bioRxiv - Microbiology 2020Quote: ... and subjected to on-column DNase-treatment using the RNase-Free DNase Set (Qiagen). cDNA synthesis ...
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bioRxiv - Microbiology 2021Quote: ... treated with RNase-free DNase I (Fermentas) and purified using a RNAeasy Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... On-column DNase digestion was performed using the RNase-Free DNase Set (Qiagen #79254).
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bioRxiv - Biophysics 2019Quote: ... column and protocol including on-column RNase-free DNase digestion (Qiagen; Cat No. 79254) and eluted into 30 μL RNase free water.
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bioRxiv - Systems Biology 2019Quote: ... DNA was then removed from the samples using a RNase-free DNase (Qiagen S.r.l.). 10 µl of the extracted RNA was reverse-transcribed using a Superscript II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Bioengineering 2019Quote: ... The plasmid DNA was extracted from bacterial cells using Endotoxin-Free Plasmid Maxiprep (Qiagen).
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bioRxiv - Genomics 2020Quote: ... Residual contaminant DNA was removed using 30 U RNase-free DNase (Qiagen CA, USA), and elution was performed with 40 μl of RNase-free water ...
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bioRxiv - Cell Biology 2019Quote: ... The samples were treated with DNAse (Rnase-Free Dnase Set Qiagen Cat. No. 79254) during RNA isolation ...
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bioRxiv - Molecular Biology 2020Quote: ... on-column digestion of DNA was additionally performed using RNase-Free DNase Set (QIAGEN) as a precautionary measure ...
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bioRxiv - Cancer Biology 2021Quote: ... a DNAse digestion step was included using the RNAse-free DNAse set (Qiagen #79254). Total RNA was isolated using the miRNeasy Mini Kit (Qiagen # 74004) ...
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bioRxiv - Microbiology 2021Quote: ... The plasmid was purified using the Endo-Free Plasmid Mega Kit (Qiagen, Germantown, MD) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: ... 18 total RNA samples were DNase treated using RNase-free DNase Set (Qiagen, 79254). Samples were submitted for mRNA-Seq at the Donnelly Sequencing Centre at the University of Toronto (http://ccbr.utoronto.ca/donnelly-sequencing-centre) ...
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bioRxiv - Systems Biology 2020Quote: ... Purification was done with a DNAse treatment RNase-Free DNase Set (Qiagen, Hilden, Allemagne) and then RNeasy MinElute Cleanup Kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2019Quote: ... with the addition of on-column DNase I digestion (Qiagen RNase-Free DNase Set). We then added 2.5 μl SUPERase-InTM RNase inhibitor (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... On-column DNAaseI treatment was carried out according to RNase-Free DNase Set (Qiagen). To construct the Illumina library for degradome analysis ...
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bioRxiv - Genomics 2021Quote: ... including an on-column DNase digestion using the RNase-free DNase Set (79254; Qiagen).
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bioRxiv - Genomics 2021Quote: ... including an on-column DNase digestion using the RNase-free DNase Set (79254; Qiagen). cDNA was prepared from 0.5-1 μg total RNA with the QuantiTect reverse transcription kit (205311 ...
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A tale of two transcriptomic responses in agricultural pests via host defenses and viral replicationbioRxiv - Genomics 2020Quote: ... On-column DNase digestion was carried out with the RNase-free DNase Kit (Qiagen), followed by a further purification step using RNeasy Mini Spin Columns (Qiagen) ...
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bioRxiv - Developmental Biology 2020Quote: ... Genomic DNA was removed by digesting with RNase-free genomic DNA eraser buffer (Qiagen), and cDNA was obtained by reverse transcription of RNA using Evo M-MLV RT Kit for PCR Kit (Accurate Biotechnology(Human)Co.,LTD ...
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bioRxiv - Cell Biology 2020Quote: ... with on-column DNase digestion with the RNase-Free DNase Set (79254, QIAGEN GmbH), both according to the manufacturer’s instructions (quick start protocol version ...
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bioRxiv - Immunology 2020Quote: ... Total RNA was subjected to on-column DNA digestion with RNase-free DNase (Qiagen) and post-isolation DNA digestion using DNA-free DNase Treatment and Removal System from Ambion (Austin ...
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bioRxiv - Cell Biology 2020Quote: ... followed immediately by a DNAse Treatment using an RNAse-free DNAse kit (79254, Qiagen). RNA was quantified using a Nanodrop and a minimum of 200 ng was used for reverse transcription cDNA synthesis ...