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Citations for Qiagen :
701 - 750 of 5289 citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... At the end of activation cells were washed twice with PBS and cell pellets were snap-frozen in RLT Plus lysis buffer (Qiagen, #1053393). Total RNA was extracted with the Qiagen’s RNeasy kit according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted for each of the persistently infected cells as well as the cell lines without NDV infection (negative control) using Qiagen RNeasy micro kit (Qiagen, Germany) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Cell lysis and RNA extraction from siRNA transfected cells were performed using the RNeasy RNA extraction kit (Qiagen Inc, Germantown, MD). 1 µg of total RNA was reverse transcribed with the High-capacity RNA-to-cDNA kit (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: E3 and E4 primary microglia were plated at 5×106 cells/well in 6-well plates and RNA was extracted from the cells using the RNEasy Plus Mini Kit (Qiagen #74136) and converted to cDNA using High-Capacity RNA-to-cDNA kit (Thermo #4387406 ...
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bioRxiv - Genomics 2019Quote: ... and the remaining cells in single-cell capture (T1, T2, and T3 samples) using the QIAamp DNA Mini kit (Qiagen, 51304), and 500 ng of DNA were used to construct exome sequencing libraries with the same reagents as the single cell DNA-seq.
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bioRxiv - Genomics 2021Quote: ... the cells were collected and total RNA was extracted from the cells using the Qiagen RNeasy® Plus Kit (Qiagen, 74134). One µg of RNA was then used to synthesize cDNA using the Bio-RAD iScript cDNA Synthesis Kit (Bio-RAD ...
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bioRxiv - Cell Biology 2019Quote: ... MCMBP depleted cells using siRNA and MCMBP-KO (clone #1 and #2) cells was isolated using RNeasy Mini Kit (Qiagen, 74104). The cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kit (Thermo Fischer Scientific ...
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bioRxiv - Genetics 2021Quote: ... S2 cells were seeded at 1 × 106 cells/ml in a 6-well plate and transfected using Effectene (Qiagen, Germantown, MD). For mobility shift assay (Figure 4) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... A pellet of 200-400 µl of animals was resuspended in 400 µl Cell Lysis Solution (Qiagen Gentra Puregen Cell kit) with 5 µl proteinase K (20 mg/ml ...
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bioRxiv - Evolutionary Biology 2022Quote: ... log-phase cells in the range of 1–2 × 108 cells using the Qiagen DNeasy® Blood and Tissue kit (Qiagen). We resuspended the genomic DNA in 10 mM Tris-HCl pH 8.5 and stored it at 4° until submission to the McDonnell Genome Institute at Washington University in St ...
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bioRxiv - Cell Biology 2023Quote: Total RNA from 1,000 H3K27me3 HI/LO β-cells or from 50,000 sorted βHI and βLO cells was extracted using the miRNeasy FFPE Kit (QIAGEN, 217504), followed by the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (E6420L) ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from CD33+ ITGA7-sorted primary AML cells (> 4x105 cells/sample) using the RNeasy Mini kit (Qiagen, 74106) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... RNAlater was removed from the sorted cells prior to RNA isolation and total RNA was isolated from the pelleted cells using the RNeasy mini kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions with some modifications ...
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bioRxiv - Microbiology 2020Quote: ... RNA from cell lysates or cell culture supernatants was extracted using the Qiagen QIAamp viral RNA mini kit (Qiagen, Valencia, CA, USA). Reverse transcription (RT ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA and DNA was extracted from the same cells from neuropathologist annotated tumour regions (>60% cancer cells) using appropriate Qiagen kits (Qiagen, Sussex, UK). Paired end strand-specific whole transcriptome libraries were prepared for 16 pairs using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs ...
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bioRxiv - Cancer Biology 2020Quote: DNA coming from CD34+ cells of the patient and Mononuclear cells of Healthy Donors was extracted by means of DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). 50ng of DNA were analysed through P056 MLPA kits (MRC Holland ...
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bioRxiv - Cell Biology 2021Quote: ... Genomic DNA was extracted from the control and the peptide-treated frozen cells using the Blood & Cell Culture DNA Midi Kit according to manufacturer’s instructions (Qiagen, ref. no. 13343). A first PCR was performed to amplify the lentiCRISPR sgRNA region using the following primers:
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bioRxiv - Cancer Biology 2020Quote: ... Genomic DNA was extracted from osimertinib-resistant cells as well as matched parental cells were extracted using a DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). We reviewed all candidate alterations identified in resistant cells as well as parental cells and considered those identified only in resistant cells as candidate acquired resistance genomic alterations to osimertinib.
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bioRxiv - Microbiology 2022Quote: RNA was extracted from purified CD4+ TN and TCM cells and from 24ST1NLESG cells using the RNeasy mini kit (Qiagen, Valencia, CA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... gDNA was isolated from 5 x 106 Colo205 cells using the Blood and Cell Culture DNA Maxi Kit (Qiagen, catalog no. 13362) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Suspension of lysed cells or tissues in RLT buffer was defrosted and cells were disrupted and homogenized using the QIAshredder system (Qiagen, cat. 79656) and total RNA was then extracted with the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: gDNA samples from the unselected control (gDNACtrl) and 2nd (gDNAScreen) selected group cells were extracted using the Blood and Cell Culture DNA Midi Kit (#13343; QIAGEN, Germantown, MD).
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from the SBMA OL cell model and the control cell model using the RNeasy Mini Kit (Qiagen, Hilden, Germany). The quality and quantity of the RNA obtained were checked using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... (2010) using commercial antibodies directed against the His-tag following the instructions of the manufacture (Qiagen, Hilden, Germany).
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bioRxiv - Cell Biology 2021Quote: Antibodies and their conditions of use are as follows: mouse anti-His (Western blot, 1:1000; 34660; Qiagen), rabbit anti-Brl1 (Western blot ...
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bioRxiv - Cell Biology 2019Quote: ... Chambers were washed with BRB80 and then were coated with mouse antibody against 6xHis-tag (1:50; Qiagen) for 10 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was extracted from sorted cells (RNeasy, Qiagen) and stored at −80°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... washed with 1 ml RNAprotect® Cell Reagent (Qiagen) and centrifuged for 10 min at 2,500 x g ...
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bioRxiv - Immunology 2022Quote: Respective cell populations were lysed in RLT buffer (Qiagen) containing 2-mercaptoethanol or TRIzol (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... cells were lysed in RLT buffer (Qiagen, Hilden, Germany) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Immunology 2022Quote: ... cells were suspended in RLT buffer (Qiagen, Valencia, CA), homogenized ...
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bioRxiv - Immunology 2022Quote: ... RNA was extracted from cell lysates (Qiagen RNeasy kit) and IFIT1 expression was measured by qRT-PCR (IDT Assay ID Hs.PT.561.20769090.g).
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bioRxiv - Genomics 2019Quote: ... Isolated PBMCs were resuspended in RNAprotect cell reagent (Qiagen) and stored at −80 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were reverse transfected with LNA GapmeRs (Exiqon, QIAGEN) at a final concentration of 50 or 100 nM (with similar results) ...
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bioRxiv - Genetics 2019Quote: ... We harvested cells in buffer RLT (Qiagen, Germantown, MD) 48 hours after transfection and estimated target gene expression relative to cells transfected with non-targeting siRNAs by RNA sequencing.
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bioRxiv - Cell Biology 2019Quote: ... and transfected into subconfluent cells using HiPerfect transfection (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Cells were lysed in QIAzol lysis reagent (QIAgen, #79306), mixed with 1/5th volume of chloroform ...
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bioRxiv - Physiology 2019Quote: ... Cells were lysed in 600 μL RLT plus (Qiagen) and Beta-mercaptoethanol (BME ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA from indicated cells was extracted by Qiagen RNeasy ...
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bioRxiv - Genomics 2021Quote: ... Cells were treated with proteinase K (#158920, Qiagen, Germany) and RNase A (#158924 ...
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bioRxiv - Immunology 2021Quote: ... cell lysate was homogenized using QIAshredder columns (Qiagen, #79654). RNA was extracted from homogenized lysate containing a range of 3×103 to 1×105 cells per sample using a ll Prep DNA/RNA Mini kit (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... 8×105 cells with the RNeasy mini kit (Qiagen) in combination with the QIAshredder system (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were scraped and lysed using QIAshredders (#79654, QIAGEN) and Qiagen RNeasy Mini kit (#74104 ...
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bioRxiv - Cancer Biology 2020Quote: RNA was harvested from cells using RNeasy kits (Qiagen), including the optional DNase I treatment step ...
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bioRxiv - Cancer Biology 2020Quote: RNA was extracted from cells using RNeasy kit (Qiagen). Approximately 2 μg of RNA from each cell line was converted to cDNA using the High-Capacity cDNA Reverse Transcription Kit (Thermo) ...
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bioRxiv - Microbiology 2021Quote: ... Bacterial cells were resuspended in a lysis buffer (Qiagen), together with an equal volume of acidic phenol and similar volume of glass beads ...
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bioRxiv - Systems Biology 2021Quote: RNA was extracted from cells using RNeasy (Qiagen, Germany). First strand cDNA was synthesized from 0.5-1 mg RNA by Superscript III First-Strand Synthesis superMix for qRT-PCR (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... cells were lysed with the RNAeasy mini kit (Qiagen) according to manufacturer’s instructions and the number of GECs were determined ...
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bioRxiv - Cancer Biology 2021Quote: Cells were harvested using RNeasy Mini Kit (Qiagen, 74106) RNA was eluted with nuclease-free water ...
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bioRxiv - Cell Biology 2021Quote: ... Cell transfections were carried out using Effectene (301425, Qiagen) as per the manufacturer’s protocol ...