Labshake search
Citations for Qiagen :
701 - 750 of 10000+ citations for Rat Insulin Like Growth Factor 1 Receptor IGF1R ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... pelleted and lysed (Qiagen RLT lysis buffer supplemented with 1% β-Mercaptoethanol. RNA was recovered using spin columns (Qiagen MicroRNAeasy® kit), with on-column DNAse I digest performed according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The qPCR was performed using the TaqMan™ RNA-to-CT™ 1-Step kit (Thermo Fischer Scientific) and was run in a RotorGene-6000-2-plex (Qiagen). PCR conditions having reverse transcription at 48’C for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... Triton X-100 1.2% and lysozyme 20mg/ml) and incubated at 37°C for 1 h and DNA was extracted with the DNeasy blood&Tissue kit (Qiagen, Courtaboeuf, France) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen, Hilden, Germany). Experiments were performed in triplicate.
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bioRxiv - Microbiology 2021Quote: ... at 37 °C for 1 h and recovered by using RNeasy MinElute Cleanup kit as recommended by the manufacturer (Qiagen, Hilden, Germany). RNA yields were estimated by employing a Qubit® Fluorometer as recommended by the manufacturer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from cultures of LSUCC0096 that had reached max cell density (~106 cells mL−1) growing in JW1 medium (10) at room temperature using a MoBio PowerWater DNA Isolation kit (QIAGEN, Massachusetts, USA) following the manufacturer’s protocols ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was extracted from the liver of an adult female Limnodynastes dumerilii dumerilii (Fig. 1) using the Gentra Puregene Tissue Kit (QIAGEN, Hilden, Germany) according to manufacturer’s instructions with the following exceptions ...
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bioRxiv - Genetics 2020Quote: ... at least ∼1 × 107 cells were collected by centrifugation and RNA was purified using an RNeasy Mini Kit (Qiagen 74104; Hilden, Germany), including on-column DNase digestion (Cat No ...
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bioRxiv - Microbiology 2020Quote: High-quality genomic DNA was obtained starting from 250 mg of each sample of table 1 using DNeasy PowerSoil Kit (QIAGEN, Hilden, Germany), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and isolated keratinocytes and fibroblasts from the skin and Wound7 (n = 5/each group) (Table 1 and Table S1) by using the miRNeasy Mini kit (Qiagen, Hilden, Germany) and prepared for library construction ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted from up to 1 gram of sediment using a modified version of the Qiagen DNeasy PowerSoil kit (Qiagen, Venlo, Netherlands), as described previously [41 ...
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bioRxiv - Molecular Biology 2022Quote: ... An aliquot of cells was diluted to OD600 0.3 and 500 µL of diluted cells was added to 1 mL of RNAprotect Bacteria Reagent provided in the RNeasy Mini Kit (Qiagen; catalog #74104). Protocols provided in the “RNAprotect Bacteria Reagent Handbook 2015” were used for RNA extraction (“Protocol 1”) ...
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Development of immortalized rhesus macaque kidney cells supporting infection with a panel of virusesbioRxiv - Microbiology 2022Quote: ... Then 1 μl of the cDNA preparation were used for quantitative PCR using a QuantiTect SYBR Green PCR kit (Qiagen, Hilden, Germany) and the Rotorgene Q platform (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNAs containing HIV-1 proviruses were recovered by infecting viruses produced in derivatives of THP-1 or SupT11 cells into SupT11 using DNeasy Blood & Tissue Kits (Qiagen, Cat# 69504). Following DpnI digestion ...
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bioRxiv - Molecular Biology 2023Quote: Total RNAs were prepared from livers of rats subjected to inhalational anesthesia with sevoflurane or desflurane for 6 hours or less than 1 minute using an RNeasy Midi Kit (QIAGEN, Venlo, Netherlands) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... DNA was isolated following the Powersoil DNeasy kit protocol with the addition of a 10 min bead beating step at 50 s-1 (Qiagen, Hilden, Germany). RNA was isolated following the Ribopure Bacteria kit protocol (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... We isolated the entire exon 1 of HTT with 115 CAG repeats from an HD patient with UltraRun® LongRange PCR Kit (QIAGEN, 206442) with supplementation of 10% DMSO under the following conditions ...
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bioRxiv - Microbiology 2023Quote: ... Each saliva sample was vortexed and 1) 500-750 μl was pipetted into a Powersoil Bead Tube (MOBIO PowerSoil DNA Isolation Kit, QIAGEN Cat #12888) for DNA extraction within two hours of collection (fresh) ...
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bioRxiv - Cancer Biology 2023Quote: ... After incubations cells were pickup in buffer RLT with 1% of β-mercaptoethanol and total RNA purification proceeded following the instructions of RNeasy Plus mini kit (Qiagen, USA). Quality control of total RNA was carried out by spectrophotometric readings of optic density (OD ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from 1 mL of each human plasma sample using the DNeasy Blood and Tissue kit (Qiagen, 69504, Hilden, Germany) according to the manufacturer protocol and recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA sequencing on the pancreatic xenografts in the orthotopic mouse model using PDAC cells (AsPC-1 ± SERPINB3) was also performed following isolation of RNA with the RNeasy Plus Mini Kit (QIAGEN, Venlo, Netherlands). Sequencing was performed on NovaSeq 6000 SP (Illumina ...
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bioRxiv - Neuroscience 2024Quote: ... Total RNA (1 μg) was used for cDNA synthesis using the Quantitate Reverse Transcription kit for cDNA Synthesis for PCR (Qiagen, Germantown, md). Real-time PCR was performed using SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... we placed filters in 2 mL tubes with beads and 1 mL TE buffer (Qiagen plasmid isolation kit, Qiagen Germantown, MD, USA) and ran them in the tissue lyser at either ½ speed or max speed for 30 sec or 1 min ...
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bioRxiv - Plant Biology 2023Quote: Genomic DNA was extracted from Tak-1 thallus according to the method described in (Tsuzuki et al., 2019) or using the DNeasy Plant Mini kit (QIAGEN, Hilden, Germany).
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bioRxiv - Genomics 2023Quote: ... Total RNA was extracted from approximately 1 g of fresh gametophytic tissue using the RNeasy Plant Mini Kit (Qiagen, Valencia, CA, USA).
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bioRxiv - Microbiology 2024Quote: Bacterial genomic DNA was extracted from 1 mL overnight cultures grown in LB media using a Qiagen DNeasy Blood and Tissue Kit (Qiagen, Germantown, MD) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Genomic DNA was then isolated from 1-5 million isolated PBMCs using the DNeasy Blood and Tissue kit (Qiagen; Cat. No. 69504) following manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... or if more than one band was present extracted from 1% TAE gel using the QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany). Nucleotide BLAST (BLASTn ...
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bioRxiv - Molecular Biology 2021Quote: ... Sodium bisulfite treatment was carried out using commercially available bisulfite conversion kits (EpiTect Fast Bisulfite Kit and EpiTect Fast LyseAll Bisulfite Kit; Qiagen) according to the manufacturer’s instructions except for the DNA denaturation step and sodium bisulfite reaction time ...
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bioRxiv - Microbiology 2024Quote: ... and the genomic DNA/RNA extractions were conducted using commercial extraction kits (DNeasy Blood & Tissue Kit for DNA or RNeasy Mini Kit for RNA, both from Qiagen) and purified with GeneJET Genomic DNA Purification Kit (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... total RNA was isolated using RNeasy Mini Kit or Micro Kit (Qiagen). Reverse transcription was performed using M-MLV Reverse Transcriptase and random primers following manufacturer’s instructions (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... RNA was extracted using a commercial kit (RNseasy Plant Mini Kit, Qiagen), followed by cDNA synthesis (iSCRIPT ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using standard column-based purification kit (QIAGEN RNeasy Kit, Cat. No. 74004). DNase treatment was applied during the purification to remove any potential DNA contamination ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted using the RNeasy Mini Kit extraction kit (QIAGEN). RNA-less and reverse transcriptase-less reactions were used as controls ...
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bioRxiv - Cancer Biology 2021Quote: ... purified with QIAquick Gel Extraction kit or QIAquick PCR Purification kit (Qiagen), ligated using T4 DNA Ligase (New England BioLabs) ...
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bioRxiv - Genetics 2020Quote: ... Plasmids were prepared using silica-membrane based kit (Plasmid Miniprep Kit, Qiagen) following the manufacturer’s instructions and quantified using Nanodrop Spectrophotometer ...
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bioRxiv - Genetics 2020Quote: ... according to the DNeasy Blood & Tissue kit and DNeasy Micro kit (QIAGEN). Total RNA was extracted from pelleted cells using the Rneasy Mini kit (QIAGEN ...
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bioRxiv - Microbiology 2019Quote: ... using viral RNA isolation kit (QIAmp Viral RNA Mini Kit Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... QIAprep Spin miniprep kit and QIAquick gel extraction kit (Qiagen, Hilden, Germany) were used according to the manufacturer’s protocol to isolate plasmids and to separate PCR products from agarose gels ...
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bioRxiv - Biochemistry 2023Quote: ... gel extraction kit and PCR clean-up kit was obtained from Qiagen, Hilden ...
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bioRxiv - Biochemistry 2022Quote: ... QIAGEN Plasmid Maxi Kits and QIAprep Spin Miniprep Kit were from QIAGEN. Zymo PUREII Plasmid Midiprep kit (D4200 ...
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bioRxiv - Molecular Biology 2023Quote: ... plasmid DNA preparation (QIAprep Spin MiniPrep Kit and Plasmid Midi Kit, Qiagen) and gel electrophoresis were performed according to the manufacturer’s instructions or using standard protocols (29) ...
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bioRxiv - Cell Biology 2023Quote: DNA was extracted using a commercial kit (DNeasy Blood & Tissue kit, Qiagen). For cDNA ...
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bioRxiv - Molecular Biology 2024Quote: ... and extracted with Jetgene mini prep kit (QIAprep Spin Miniprep Kit, QIAGEN). The correctness of constructions was verified by sequencing (Eurofins) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Qiaspin Miniprep Kit (Qiagen) and Monarch PCR & DNA Cleanup Kit (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... RNeasy Mini kit (Qiagen) or miRNeasy Mini kit (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... RNeasy Mini Kit (Qiagen), and DNAse I kit (Qiagen) ...
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bioRxiv - Immunology 2022Quote: RNeasy Mini Kit (Qiagen) was used for RNA isolation ...
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bioRxiv - Immunology 2021Quote: AllPrep Mini kit (Qiagen) was used for RNA extraction according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA of each sample was extracted using TRIzol Reagent (Invitrogen)/RNeasy Mini Kit (Qiagen). Control samples were collected from an antibiotic-free culture ...