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Citations for Qiagen :
701 - 750 of 1827 citations for Methionine aminopeptidase 1 METAP1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... lymphophilum API-1 were extracted using the QIAamp PowerFecal Pro DNA kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 μg of purified RNA was reverse transcribed using RT2 First Strand Kit (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: NHEKs were transfected at 50-70 % confluency with 1 µL HiPerfect transfection reagent (Qiagen) and 5 nM of either AhR-specific “SilencerSelect” siRNA (s1199 ...
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated from 1×107 cells with the RNeasy® Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: RNA was extracted from MDM or THP-1 cells using RNAeasy mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The qPCR mix (10 μL) contained 1× Rotor-Gene SYBR green PCR mix (Qiagen), 500 nM each primer ...
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bioRxiv - Immunology 2021Quote: ... Periplasmic fraction was incubated with 1 mL pre-washed Ni-NTA agarose (30230, Qiagen) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted using the RNeasy kit and treated with DNase 1(Qiagen). Three donor samples of human RPE/choroid were processed for RNAseq ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA was extracted from 1-2 million cells using RNeasy Mini Kit (QIAGEN) using QIAcube (QIAGEN) ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 µL of bisulfite-converted DNA was amplified with HotStar Taq DNA polymerase (QIAGEN) in a 25 µL reaction using the primers designed with MethPrimer [107] and listed in Supplementary Table S2 ...
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bioRxiv - Microbiology 2019Quote: Total DNA was isolated from 1×108 cells using Puregene Core Kit A (Qiagen). Free minicircles and total kDNA content were analyzed as previously described (Bruhn ...
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bioRxiv - Cancer Biology 2019Quote: ... for 1 h at 37 °C and purified using QIAquick PCR Purification Kit (Qiagen). The pLVX-Puro lentiviral expression vector (Clontech ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted from 1 million mESCs using DNeasy Blood & Tissue Kit (Qiagen, 69506) and RNA was extracted from 450,000 HEK293T cells using the Direct-zol RNA MicroPrep Kit (Zymo ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from 1 ml of the slurry using a commercial kit (Qiagen).
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bioRxiv - Neuroscience 2020Quote: ... or its mutants (200 ng) and GCaMP6 (1 μg) using the Effectene reagent (Qiagen). The human orthologue of the mouse splice variant TRPM3α2 in the pCDNA3.1(+ ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μg of RNA was retrotranscribed in cDNA with QuantiTect Reverse Transcription Kit (Qiagen), followed by a PCR amplification of the subsequent transcripts ...
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bioRxiv - Cell Biology 2021Quote: ... The crude PCR product was gel purified from 1% agarose by spin column (Qiagen), and 200 ng of the purified amplicon were end-labeled using 10 µCi of γ-32P ATP (Amersham ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was prepared from 1 μg RNA using a Quantitect Reverse Transcription kit (Qiagen) and diluted 1:20 in DEPC-treated water ...
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bioRxiv - Biochemistry 2020Quote: ... but with an anti-His antibody (Qiagen, #34660 at a dilution of 1:5000) as primary ...
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bioRxiv - Plant Biology 2021Quote: ... swc6-1 and pfd3,5 seedlings (three biological replicates) using RNeasy Plant Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... 1 μg of total RNA was reverse transcribed with Quantitect Reverse Transcription Kit (Qiagen) using oligo(dT) ...
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bioRxiv - Microbiology 2020Quote: ... served as hosts for derivatives of plasmids pGEX-6P-1 (Cytiva) and pQE30 (Qiagen), respectively ...
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bioRxiv - Microbiology 2021Quote: ... Each PCR contained 25 µL of 1 × Taq PCR Mastermix (Qiagen GmbH, Hilden, Germany), final concentration of 0.2 µM of each primer ...
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bioRxiv - Microbiology 2020Quote: ... 1 μL of template DNA and the Qiagen PCR multiplex PCR kit (Qiagen, France) on a T100TM Thermal cycler (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: rhGAA secreted from D532 was purified using 1 mL Ni-NTA agarose resin (Qiagen) according to the manufacturer’s instructions and as described previously [27] with slight modification ...
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bioRxiv - Genomics 2022Quote: ... The product was purified from a 1% agarose gel (QIAquick Gel Extraction Kit, Qiagen).
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bioRxiv - Microbiology 2022Quote: ... The resulting supernatant was supplemented with 1 mL pre-equilibrated Ni-NTA resin (Qiagen) and 10 mM imidazole and stirred for 30 min at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... and homogenized for 1 cycle with a Bead Beater TissueLyser II (QIAGEN, Germantown, MD) at 25 Hz for 1.5 min ...
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bioRxiv - Microbiology 2022Quote: ... Clarified lysate was added to 1 mL of washed Ni-NTA bead slurry (Qiagen) and incubated for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Blots were probed with antibodies against His6 (penta-His Qiagen catalog #34460; 1:10,000), FLAG2 (Sigma catalog #A8592 ...
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bioRxiv - Immunology 2019Quote: ... Cells were sorted into PBS/1% FBS before resuspension in Buffer RLT (QIAGEN, USA) or directly into Buffer RLT with 1% 2-β-mercaptoethanol (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant GST-Dnmt5(1-150)-6xHis was purified with Ni-NTA agarose resin (Qiagen), measured by A280 (ε = 66,030 cm-1 M-1) ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant MBP-Dnmt5(1-150)-6xHis was purified with Ni-NTA agarose resin (Qiagen), measured by A280 (ε = 89,590 cm-1 M-1) ...
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bioRxiv - Microbiology 2019Quote: ... The Ni-NTA matrix was loaded on a prepared 1 ml Polypropylene column (Qiagen) and a filtered P ...
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bioRxiv - Paleontology 2019Quote: ... plasmid minipreps were purified with a QIAprep Miniprep Kit (Qiagen, chimpanzee 1 and 2), and RBC Miniprep Kit (YPD100 ...
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bioRxiv - Genomics 2019Quote: ... Lysis reactions were neutralized by adding 1 μL neutralization buffer (stop solution, Qiagen, Germany). Both ...
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bioRxiv - Neuroscience 2020Quote: ... cDNAs were synthesized from ∼1 µg total RNA using QuantiTect Reverse Transcription kit (QIAGEN) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... 1 mL of each culture was added to 2 mL RNAprotect Bacteria Reagent (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... 0.5% (v/v) Triton X-100) and 1 mg/ml Proteinase K (QIAGEN, Germany). The solution was incubated at 55°C for 15 min ...
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bioRxiv - Microbiology 2019Quote: ... mixed at a ratio of 1:10 with QIAzol lysis reagent (Qiagen, Cat: 79306) and homogenized by bead beating (FastPrep-24 Instrument ...
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bioRxiv - Genomics 2021Quote: ... 1 mM EDTA (pH 7.5) and purified on a Genomic-tip 100/G (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and stored in either 1 ml Cell Lysis Solution (Gentra Puregene Kit, Qiagen, USA), Queen’s buffer ...
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bioRxiv - Microbiology 2020Quote: ... HIV-1 RNA was analyzed using the QuantiTect SYBR Green RT-PCR kit (Qiagen) in a LightCycler 480 (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... The soluble extracts were applied to a 1 ml of Ni2+- NTA-agarose (Qiagen) column that had been equilibrated with buffer A containing 10 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: RNA was extracted from approximately 1×106 cells using the RNeasy Mini kit (Qiagen). DNA was digested using the TURBO DNA-free Kit (Ambion) ...
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bioRxiv - Microbiology 2020Quote: ... were homogenized 7 min at 50 pulses s-1 with the Tissuelyser LT (QIAGEN) prior to incubation in lysis buffer for 1 h at 60°C for increasing cell lysis ...
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bioRxiv - Immunology 2021Quote: ... Each tissue was collected into a microcentrifuge tube containing 1 mL of Qiazol (Qiagen) and autoclaved glass beads ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized from 1 μg total RNA using the QuantiTect RT kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... 1 x 75 bp next generation sequencing of placental microRNA was performed by Qiagen Genomic Services (Frederick ...
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bioRxiv - Biochemistry 2021Quote: ... precleared lysates were mixed with 1 ml Ni-NTA-agarose beads (Qiagen; Hilden, Germany) and incubated for 1 h rotating at 4°C ...