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Citations for Qiagen :
701 - 750 of 2234 citations for Aminomethylphosphonic Acid 13C 99%;15N 98%;Methylene D2 98% 100 Ug Ml H2O since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: Total RNA was extracted from 100 oocytes with RNeasy micro purification kit (Qiagen). The first strand cDNA was generated with M-MLV first strand cDNA synthesis kit (Takara) ...
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bioRxiv - Neuroscience 2021Quote: ... Fly heads were homogenized in 100 µl RTL buffer from RNeasy kit (Qiagen) using a motorized pestle ...
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bioRxiv - Microbiology 2021Quote: ... DNA pellets were collected by centrifugation and resuspended in 100 μl EB (Qiagen) by vigorous shaking for 10 min at 55°C ...
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bioRxiv - Plant Biology 2021Quote: The samples (100 mg) were powdered using a TissueLyser II (Qiagen, Hilden, Germany). The powdered samples were used to quantify the starch ...
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bioRxiv - Plant Biology 2020Quote: ... The extracted DNA was eluted in 2 x 100 μL EB buffer (Qiagen), cleaned and concentrated with the Clean and Concentrator-5 kit (Zymo Research ...
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bioRxiv - Cell Biology 2020Quote: ... Transfections were carried out with 100 nM miRNA-221-3p mimic (Qiagen MSY0000278), anti-miR-221-3p (Qiagen MIN0000278 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... following the manufacturer’s protocol and eluted in 100 μL of TE buffer (Qiagen).
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bioRxiv - Cell Biology 2022Quote: ... Islets were frozen at −80°C in 100 μL of RLT buffer (Qiagen) with beta mercaptoethanol (1%) ...
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bioRxiv - Biochemistry 2020Quote: ... each 100 μl reaction was purified using a QIAquick PCR Purification Kit (Qiagen) according to the manufacturer’s protocol and eluted in 30 μl of nuclease-free water ...
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bioRxiv - Microbiology 2021Quote: ... gDNA extraction was performed using the Genomic-tip DNA 100/G Kit (Qiagen), following the Tissue Sample method ...
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bioRxiv - Microbiology 2021Quote: ... pH 8.0) and a 100-bp GelPilot® DNA Molecular Weight Marker (Qiagen).
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bioRxiv - Genomics 2020Quote: ... Pre-capture libraries with less than 100 ng of double-stranded cDNA (n=5; PT0017_Qiagen_20ng_XTHS, PT0017_Covaris_20ng_XTHS, PT0017_Qiagen_20ng_Illumina ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we incubated samples with Qiagen Solution C3 (Qiagen DNeasy PowerSoil 12888-100-3) to remove PCR inhibitors ...
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bioRxiv - Molecular Biology 2022Quote: ... Product longer than 100 nt was recovered using QIAquick Gel Extraction Kit (Qiagen) after separation of the ligation products with TBE-Urea 10% Gel (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was extracted using the DNeasy PowerLyzer PowerSoil Kit (12855-100, QIAGEN) according to manufacturer’s protocol with a minor modification ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 100 µl of lysis buffer from the QIAGEN RNeasy mini kit (Qiagen, #74104) was added to each well to lyse the cells ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we incubated samples with Qiagen Solution C3 (Qiagen DNeasy PowerSoil 12888-100-3) to remove PCR inhibitors ...
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bioRxiv - Physiology 2023Quote: RNA was harvested with 100 µL of QIAzol Lysis Reagent (Qiagen, Hilden, DE) per well of an MEA plate ...
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bioRxiv - Cancer Biology 2022Quote: A 100 µL slurry of 5% Ni-NTA magnetic beads (Qiagen, part# 36113) was placed in 12 1.5 mL Eppendorf tubes and the tubes were placed in a magnetic stand and the supernatant was removed ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were precipitated by adding 100 μL of a protein precipitation solution (Qiagen). Samples were centrifuged for 5 min at 13000 rpm ...
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bioRxiv - Microbiology 2023Quote: ... were transfected with either 100 nM of the specific YTHDF1 siRNA (SI00764715, Qiagen) or 100 nM siGENOME non-targeting siRNA (Dharmacon ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The samples were centrifuged and the supernatant mixed with 13 mL of a modified PB buffer (12.6 mL PB buffer (Qiagen), 6.5 μL Tween-20 ...
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bioRxiv - Plant Biology 2021Quote: ... the protein was removed by 1.1 μL of proteinase K (20mg/ml) at 45° C for 1h and RNA by 2μL of RNaseA (1mg/ml) (Qiagen) digestion at 37°C for 1h ...
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bioRxiv - Molecular Biology 2020Quote: ... washed twice in PBS and DNA was labeled with propidium iodide (50 μg/ml) in presence of RNase A (0.2 mg/ml, Qiagen) and Triton X-100 (0.1%) ...
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bioRxiv - Microbiology 2019Quote: ... Bacteria were treated with lysostaphin (2.5 mg/mL) and lysozyme (50 mg/mL) prior to RNA extraction using the RNeasy Plus Mini Kit (Qiagen). RNAs were treated with TURBO DNA-free™ (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... were homogenised in a Precellys 24 homogeniser with CK28 Hard tissue homogenizing 2.0 ml tubes (Bertin) and 1 ml of RLT buffer (Qiagen) supplemented with 1%(v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... and DNA was labeled with propidium iodide (50 μg/ml) in the presence of RNase A (0.2 mg/ml, Qiagen) and Triton X-100 (0.01% ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was loaded onto a 10 mL Ni-NTA Superflow cartridge (connected two 5 mL cartridges) (30761, Qiagen). The cartridge was washed with 100 mL R buffer containing 200 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... Cells suspended in lysis buffer were transferred to a 2 mL screw cap tube that contained 0.5 mL of 0.1 mm disruption beads for bacteria and 0.7 mL QIAzol™ Lysis Reagent (Qiagen; 79306). Cells were lysed via mechanical disruption using a bead beater for four rounds of 30 s each ...
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bioRxiv - Systems Biology 2023Quote: ... A culture volume equal to 3 mL of OD = 1 was added to 6 mL RNAprotect Bacteria Reagent (Qiagen), vortexed ...
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bioRxiv - Immunology 2019Quote: ... longitudinal muscle/myenteric plexus preparations were homogenized in a 2 ml eppendorf containing 1 ml Trizol and a single 5 mm steel bead in a TissueLyzer (Qiagen) for 3 min at 30 Hz ...
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bioRxiv - Immunology 2019Quote: ... mucosal scrapings and feces were homogenized in a 2 ml eppendorf containing 1 ml of DNA extraction buffer and a single 5 mm steel bead in a TissueLyzer (Qiagen) for 3 min at 30 Hz ...
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bioRxiv - Microbiology 2021Quote: ... 1 g yeast extract in 750 ml of 30 kDa filtered seawater and 250 ml of Milli-Q water) using the DNeasy Blood & Tissue kit (Qiagen) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: The supernatant was carefully removed to a 50-mL sterile tube and mixed with 2 mL volume of 50% Ni-NTA resin (Qiagen) which was pre-equilibrated in the buffer A ...
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bioRxiv - Microbiology 2020Quote: ... and the cycle-threshold CT translated to bacterial load (estimated CFU per mL (eCFU/mL) using a standard curve on a Rotor gene Q 5plex platform (Qiagen). The cut-off for TB-MBLA positivity is a 30 CT value that corresponds to 1.0 log10 eCFU/mL ...
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Unraveling the functions of uncharacterized transcription factors in Escherichia coli using ChIP-exobioRxiv - Systems Biology 2021Quote: ... Transcripts were stabilized by mixing 3 mL of cell cultures at the mid-log phase with 6 mL of RNAprotect Bacteria Reagent (Qiagen). Samples were immediately vortexed for 5 sec ...
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bioRxiv - Microbiology 2022Quote: ... from the top A horizon were immediately collected for DNA extractions in 15 mL tubes containing 5 mL Lifeguard Soil Preservation Solution (QIAGEN) using chilled soil-processing trays ...
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bioRxiv - Cell Biology 2021Quote: ... The remaining lysate was transferred to a 15 ml falcon tube and 1 ml of a 50% slurry consisting of NiNTA agarose beads (Qiagen) in resuspension buffer was added per 10 ml of lysate ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were removed and the remaining cell pellet (500 μl) was transferred into a 15 ml tube containing 2.5 ml RNAprotect® Cell Reagent (Qiagen). The cells were resuspended by vortexing ...
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bioRxiv - Microbiology 2021Quote: ... Mosquito bodies and legs and wings were put into a 2 ml round-bottom tubes containing 250 ml of PBS and a steel ball (Qiagen). Samples were homogenized using a TissueLyser (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... in 96 flat bottom plates in the presence or absence of PMA (200ng/ml) and ionomycin (300ng/ml) were lysed in 100Sµl lysis buffer containing TCL buffer (Qiagen, 1031576) containing 1% β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2021Quote: ... clones were isolated from a 5 ml High Salt (HS) Luria Bertani (LB) culture containing ampicillin (50 μg/ml) using the QIAprep Spin Miniprep Kit (Qiagen). Inserts in pGEM-T Easy were sequenced at Aberystwyth University’s Translational Genomics Facility and the sequence trace was analysed using FinchTV 1.4.0 software [45] ...
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bioRxiv - Biochemistry 2021Quote: ... and early stationary phase (OD = 1.0-1.3).1 mL sample of each culture was directly transferred to 2 mL RNAprotect cell reagent (Qiagen, Hilden, Germany). The samples were vortexed 5 sec ...
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bioRxiv - Biophysics 2023Quote: ... and precipitated with ammonium sulfate as in Niu et al.49.The precipitate was dissolved in 30 ml buffer C and loaded onto a 5 ml column of Ni-NTA-agarose (Qiagen) pre-equilibrated in buffer C ...
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bioRxiv - Microbiology 2023Quote: ... Pellets were washed in 2 mL cold 10 mM NaCl + 4 mL cold RNAprotect Bacteria Reagent (Qiagen Cat. No. 76506) and repelletted at 4,255 x g ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were collected continuously into 50 mL conicals containing 20 mL RNAlater (saturated ammonium sulfate, 20mM EDTA, pH 5.2; QIAGEN #76106) and a stirring magnetic stir disc (V&P Scientific #772DP-N42-5-2) ...
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bioRxiv - Molecular Biology 2023Quote: ... fungal DNA was extracted independently from 1 mL of crude conidial suspensions (between 108 and 109 spores mL-1) using the DNeasy 96 Plant Kit (Qiagen) following the manufacturer’s instructions (Gautier et al. ...
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bioRxiv - Biochemistry 2024Quote: Total RNA was isolated from bacterial cells using the RNeasy mini kit (5 mL culture) and RNeasy maxi kit (50 mL culture) (Qiagen). RNA concentrations were measured using a Nanodrop spectrometer (ThermoScientific) ...
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bioRxiv - Cell Biology 2020Quote: ... The rest of the supernatant was added to 100 µl Ni-NTA agarose (Qiagen) slurry and incubated for 2.5 hours at 4 °C on a rotary wheel ...
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bioRxiv - Microbiology 2020Quote: ... 100 bootstrap replications) was constructed in CLC Main Workbench (v7.6.3, CLCbio, Qiagen, Aarhus, DK). The tree was visualised by use of the FigTree software v1.4.3 (http://tree.bio.ed.ac.uk/software/figtree/) ...