Labshake search
Citations for Qiagen :
701 - 750 of 2506 citations for 7H Benzocyclohepten 7 one 2 amino 5 6 8 9 tetrahydro 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... reverse transcription and amplification of the S gene were done with the one-step QuantiFast Sybr Green RT-PCR mix (Qiagen) as follows ...
-
bioRxiv - Microbiology 2020Quote: ... The SVIP-MPv2 assay was developed and validated as a one-step procedure using the QuantiNova Probe RT-PCR Kit (Qiagen) with final concentrations of primers (1.4μM each ...
-
bioRxiv - Microbiology 2022Quote: ... RNA was transcribed using a T7 RNA polymerase reaction and subsequently DNAse-treated for one hour followed by purification using a RNeasy kit (Qiagen) as per manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... Gene expression was evaluated by qPCR using the ONE-step QuantiTect Probe RT-PCR Kit (Qiagen, Cat No./ID: 204445). For each reaction 2 μL of RNA were used ...
-
bioRxiv - Cell Biology 2019Quote: Confluent FBs from three different donors for each cell type were grown in one well of a 12-well plate and total RNA was purified using the RNeasy Mini Kit (Qiagen) with an on-column DNase treatment ...
-
bioRxiv - Microbiology 2020Quote: ... 5 µl RNA was used in a one-step real-time RT-PCR E assay33 using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
-
bioRxiv - Pathology 2021Quote: ... and from lung and/or heart tissues of seropositive rodents were used to amplify viral genome by RT- PCR using the One Step RT-PCR kit (QIAGEN) followed by nested or heminested PCRs (Taq Pegasus ...
-
bioRxiv - Microbiology 2020Quote: ... EAV genome copy number was determined by one step RT-qPCR using the QuantiTect™ Virus + ROX Vial Kit (Qiagen). Each RNA sample was tested in triplicate ...
-
bioRxiv - Cell Biology 2020Quote: ... The RNA concentration was measured using Geneflow Nanophotometer and RT-qPCR was performed with one-step QuantiFast SYBR Green qPCR kit (Qiagen) using 50ng of RNA in each sample ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... reverse transcription and amplification of the S gene were performed using the one-step QuantiFast Sybr Green RT-PCR mix (Qiagen) as follows ...
-
bioRxiv - Genomics 2021Quote: ... RNA was extracted using a QIAGEN RNeasy Mini Kit followed by two rounds of DNase digestion: one on-column using the QIAGEN RNase-Free DNase Set (79254, QIAGEN); and one off-column using Invitrogen’s TURBO DNA-free Kit (AM1907 ...
-
bioRxiv - Genetics 2021Quote: Genomic DNA was extracted from one wild-type control and three patient cell lines using DNeasy Blood and Tissue kit (Qiagen). Specific primers were designed to cover approximately 500 base pairs around the mutations and the regions were PCR-amplified using Qiagen Multiplex PCR kit (Qiagen) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... RNA from the original material used for next-generation sequencing of the serum and passage 3 cultured MRI103 virus was used as the template for amplification using the One-Step Ahead RT-PCR kit (Qiagen).
-
bioRxiv - Immunology 2021Quote: ... Crystals of SB16-RBD and SB45-RBD complexes and Sb16 alone were observed within one week using Protein Complex (Qiagen) and Wizard Classic 4 (Rigaku) ...
-
bioRxiv - Immunology 2021Quote: ... 5 μl RNA was used in a one-step real-time RT–PCR E assay using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
-
bioRxiv - Immunology 2021Quote: ... Five μl RNA was used in a one-step real-time RT-PCR using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
-
bioRxiv - Immunology 2020Quote: ... and a volume that equated to 17.5 mg of tissue was extracted using the BioSprint™96 One-For-All vet kit (Qiagen) and Kingfisher Flex platform as per manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were pelleted at low speed (~1000 RPM) and lysed with one mL RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen).
-
bioRxiv - Immunology 2022Quote: ... One volume of 70% ethanol was added and then transferred to a column from the RNeasy mini kit (Qiagen, 74104). RNA isolation was performed according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were vortexed and frozen for at least one day at −80° before being thawed on ice and processed for RNA using an RNeasy Mini Kit (Qiagen). Purified mRNA was converted to cDNA with the High Capacity cDNA Reverse Transcription kit (Life Technologies ...
-
bioRxiv - Neuroscience 2022Quote: Vector genomes present in each treated animal was quantified after preparation of genomic DNA from one brain hemisphere with the AllPrep96 DNA/RNA Kit (Qiagen). qPCR with primers and a probe directed against the hSyn promoter sequence (forward 5’-ATGAGTGCAAGTGGGTTTTAGGA-3’ ...
-
bioRxiv - Molecular Biology 2023Quote: ... Viral RNA was extracted one from (Used in detection limit evaluation) using viral RNeasy® Mini-kit (Qiagen, Hildan, Germany). Extracted RNA was stored at −70°C until further use.
-
bioRxiv - Bioengineering 2023Quote: ... followed by three rounds of washes with sterile PBS + PI for 45 mins, then subjected to one round of incubation with DNase I (4716728001, MilliporeSigma) + RNase A (19101, Qiagen) + PI for 72 hours ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cell free DNA (cfDNA) extraction and library preparation was performed on plasma samples (QIAseqTM cfDNA All-in-One Kit (QIAGEN)) ...
-
bioRxiv - Microbiology 2023Quote: ... a single GFP+ or GFP-J-Lat 11.1 cell was sorted directly into each well of a 96-well PCR plate containing the pre-amplification mastermix consisting of 1 μL One-step RT-PCR enzyme (QIAGEN), 5 μL 5× One-step RT-PCR buffer (QIAGEN) ...
-
bioRxiv - Cell Biology 2023Quote: ... Transcript-specific primers were designed to span introns and the spcar3 mutation site and used in one-step RT-PCR reactions (210210, OneStep RT-PCR kit, Qiagen). Primer sequences for qRT-PCR are listed in Table 1 ...
-
bioRxiv - Physiology 2023Quote: One thyroid lobe was used for total RNA extraction according to the manufacturer’s protocol (RNeasy Micro Kit, Qiagen, Hilden, Germany). RNA concentration and purity were determined by spectrophotometry (ratio 260/280 nm) ...
-
bioRxiv - Microbiology 2023Quote: ... and Chl523R (5’ CCY YMC GTA TTA CCG CAG CT 3’) targeting the 16S rRNA gene on a QIAcuity One digital PCR device (Qiagen) as recommended by the manufacturer ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... total RNA of each developmental stage was isolated from one to three biological replicates using the RNeasy Micro Kit (Qiagen). Library preparation for stranded polyA-based mRNA was then performed using the TruSeq Stranded mRNA Library Prep Kit (Illumina) ...
-
bioRxiv - Pathology 2024Quote: ... by running one homogenization cycle (50 Hz oscillation frequency (50 cycles/s) for 4 minutes) in a Tissue lyser LT (Qiagen). Tubes were then centrifuged at 5000 rpm for 5 min ...
-
bioRxiv - Microbiology 2024Quote: ... One hundred microliters (uL) PBS was added to NW pellets followed by 280uL AVL lysis buffer (Qiagen, Germantown, MD, USA). Two to three mL blood was collected from the cranial vena cava in an SST tube for serum separation ...
-
bioRxiv - Microbiology 2024Quote: ... corresponding to the three extraction protocols we used: (NM-1) one negative control for the kit DNeasy Blood and Tissue (Qiagen) used for mosquito DNA extraction ...
-
bioRxiv - Cancer Biology 2020Quote: ... 2 μl of inner PCR product was added to 2 μl 10x PCR buffer (Qiagen, Cat# 203203), 0.4 μl of 10 μM dNTP mix ...
-
bioRxiv - Microbiology 2022Quote: ... of the hamsters at 2 dpi or 2 days post-exposure (exposed naïve) using RNeasy kit (Qiagen) for viral load quantification and calculation of the Omicron:Delta ratio ...
-
bioRxiv - Systems Biology 2021Quote: ... 2 mL samples were mixed into glass tubes containing 2 volumes of RNAprotect bacteria reagent solution (Qiagen). After mixing and incubating for 5 min at room temperature ...
-
bioRxiv - Microbiology 2022Quote: SARS-CoV-2 whole-genome sequencing was performed by using QIAseq DIRECT SARS-CoV-2 Kit (QIAGEN). The quality of paired-end reads obtained from MiSeq sequencing was analyzed by using Qiagen CLC Genomics Workbench 22.0.1 and the Identify ARTIC V3 SARS-CoV-2 Low Frequency and Shared Variants (Illumina ...
-
bioRxiv - Microbiology 2019Quote: ... 5-10 μg RNA was DNAse treated (Qiagen) and rRNA was depleted (MICROBExpress ThermoFisher) ...
-
bioRxiv - Microbiology 2021Quote: ... and 5 mm diameter stainless steel beads (QIAGEN) were added to each sample pool ...
-
The gut hormone Allatostatin C regulates food intake and metabolic homeostasis under nutrient stressbioRxiv - Physiology 2020Quote: ... and 5-mm stainless-steel beads (Qiagen #69989) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... 5 µL Proteinase K (20 mg/mL, Qiagen) and 100 µl 10% SDS (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... in 5-ml polypropylene columns (no. 34964; Qiagen), washed with 50 mM Na2HCO3 ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... and 5-mm stainless steel beads (Qiagen #69989). RNA purification was performed using the NucleoSpin RNA kit (Macherey-Nagel ...
-
bioRxiv - Microbiology 2021Quote: ... in a 5 ml tube (Qiagen, DNAse/RNAsefree). The sediment samples were kept at 4 °C until the next day and then stored at −80 °C ...
-
bioRxiv - Immunology 2020Quote: ... 5×106 PBMCs were resuspended in RLT (Qiagen) and incubated at room temperature for 10 min prior to storage at –80°C ...
-
bioRxiv - Cell Biology 2021Quote: ... si-USP36-1 (5’-CAAGAGCGUCUCGGACACCUA-3’; Qiagen, Microsynth), si-USP36-2 (5’-UCCGUAUAUGUCCCAGAAUAA-3’ ...
-
bioRxiv - Physiology 2020Quote: ... with 5 mm beads in RLT buffer (Qiagen) containing 1% β-mercaptoethanol ...
-
bioRxiv - Cell Biology 2021Quote: ... + 5% beta-mercaptoethanol using a bead mill (Qiagen). Samples were heated at 95° for 5 minutes to denature proteins ...
-
bioRxiv - Microbiology 2022Quote: ... containing 5 mm diameter stainless steel beads (Qiagen) and 150 μL DMEM supplemented with 2% FBS and homogenized using a TissueLyser II (2 cycles at 30 Hz ...
-
bioRxiv - Microbiology 2022Quote: ... 5 μL of 2U/μL Turbo DNase (Qiagen), and 1 μL of 10 mg/mL RNase A (Qiagen) ...
-
bioRxiv - Physiology 2023Quote: ... Arf6 siRNA sequence: 5’- CAACGTGGAGACGGTGACTTA-3’ (QIAGEN SI02757286). QIAGEN All Stars Negative sequence was used as control.