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Citations for Qiagen :
701 - 750 of 3295 citations for 6 CHLORO 2 METHYLIMIDAZO 1 2 A PYRIDINE 3 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Obtained lists with significantly changed genes (p<0.05, fold-change ≥2) were used in Ingenuity Pathway Analysis (IPA, Qiagen).
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bioRxiv - Immunology 2023Quote: ... pH 6.0) and homogenized using a sterile 5mm stainless steel bead in a Tissue Lyser 2 (Qiagen, Hilden, Germany) for 90 seconds at 1/60Hz ...
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bioRxiv - Immunology 2023Quote: ... The supernatant containing target proteins was passed through a polypropylene column containing 2 ml Ni-NTA resin from Qiagen. After the target protein bound to the Ni-NTA resin ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 24,000 g for 30 min and applied to a 2 mL column bed of Ni-NTA resin (Qiagen) for purification by IMAC ...
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bioRxiv - Microbiology 2023Quote: Total RNA was extracted from 2 g of rhizosphere soil using the RNeasy PowerSoil Total RNA Kit (QIAGEN, Canada) and 0.5 g roots using RNeasy Plant Mini Kit (QIAGEN ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse thyroid tissue was homogenized in buffer RLT using TissueLyser II (Qiagen; 2x 2 min cycles at 30 Hz) and 5 mm stainless steel beads ...
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bioRxiv - Molecular Biology 2023Quote: ... The tubes were shaken for 2×45 seconds at 30 Hz using a Tissue Lyser (TissueLyser II, Qiagen, Germany). After homogenization ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed 2 times with 1X PBS and RNA was isolated using a RNAeasy Plus Mini Kit (Qiagen).
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bioRxiv - Neuroscience 2023Quote: ... Those included UniSpike 2 and 4 from the QIAGEN RNA Spike-In Kit (Cat. No.: 339390, QIAGEN, Hilden, Germany), which were used to monitor RNA isolation efficacy ...
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bioRxiv - Microbiology 2023Quote: ... The pure genomic DNA was sheared by ultrasound and 2-8 kb fragments were extracted from a 0.7% agarose gel using the Gel Extraction Kit (Qiagen). The gel-recovered fragments were ligated to the linearized PZE12 vector (prime 3,4 ...
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bioRxiv - Genomics 2023Quote: MII oocytes and 2-cell embryos were individually lysed and flash-frozen in 5 μl RLT Plus buffer (Qiagen) and stored at −80°C until further use ...
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bioRxiv - Microbiology 2023Quote: ... containing a sterile 4.5mm ball bearing and homogenized with 2 rounds of bead-beating (2m30s, 30 beats/s) in a TissueLyserII (Qiagen). The lysate was then transferred to a new tube and 1/5 volume of chloroform (Avantor ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Genomic DNA was extracted from 2 mL of each of the nine overnight cultures (DNeasy Blood & Tissue Kit; Qiagen). Library preparation and sequencing was performed by Novogene Europe (Cambridge ...
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bioRxiv - Cell Biology 2023Quote: ... for 1h at 37 °C (2 units per sample) followed by purification with the RNeasy miniElute Cleanup kit (Qiagen).
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bioRxiv - Cancer Biology 2024Quote: ... Mouse thyroid tissue was homogenized in buffer RLT using TissueLyser II (Qiagen; 2x 2 min cycles at 30 Hz) and 5 mm stainless steel beads ...
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bioRxiv - Cell Biology 2023Quote: ... individual organoids were washed 2 times with ice-cold PBS and subsequently dissociated with 350µL of lysis buffer (RLT Buffer, QIAGEN). Samples were stored at - 80°C until they were delivered to USEQ Utrecht Sequencing Facility (Utrecht ...
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bioRxiv - Molecular Biology 2021Quote: The ER-I-PpoI cells treated with 4-OHT (2.5 μM) for 1 hour were collected for extraction of the total nucleic acids using DNeasy Blood & Tissue Kits (Qiagen) or PureLink(tm ...
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bioRxiv - Molecular Biology 2020Quote: ... Purification of p53-(1-73) from the clarified cell lysate was accomplished using a Nickel Nitrilotriacetic Acid (Ni-NTA, Qiagen) column purification and 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... viral nucleic acids were extracted using the QIAamp Viral RNA Mini Kit (QIAGEN GmbH, QIAGEN Strasse 1, 40724 Hilden, Germany), which purifies RNA and DNA ...
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bioRxiv - Genetics 2022Quote: ... Midi, 3-4 ml of blood) and Puregene (0.3-1 ml of blood, manual extraction) (Qiagen, Cat# 1057048 ...
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bioRxiv - Cell Biology 2021Quote: ... 3-month-old zebrafish (1 fish/sample) using the RNeasy Mini Kit (Cat. No. 74104; Qiagen) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... KIF4A siRNA 5’-CAGGTCCAGACTACTACTC-3’ against the 3’-UTR was obtained from QIAgen, and an optimised siRNA pool for KIF22 (KID ...
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bioRxiv - Physiology 2020Quote: ... Erythrocytes were lysed for 3 minutes with 3 ml of EL buffer (Qiagen). After Fc blocking for 20 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... 3’mRNA-seq libraries were prepared using QIAseq UPX 3’ Transcriptome Kit (QIAGEN). In brief ...
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bioRxiv - Genetics 2020Quote: ... coli strains expressing (His)6-Clr4 and (His)6-Swi6 and allowed to bind at 4°C overnight to Ni-NTA resin (Qiagen; binding capacity 5-10mg protein/ml resin) ...
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bioRxiv - Microbiology 2023Quote: ... The SCGs cultured in 6-well dishes and one entire 6-well was harvested and pooled together for RNA isolation (RNeasy, Qiagen) per sample ...
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bioRxiv - Cell Biology 2020Quote: ... Kinetochore proteins were conjugated to these beads using antibodies that recognize specific proteins or their tags: biotinylated anti-His-tag antibodies (6 µg ml−1, Qiagen), biotinylated anti-GFP antibodies (20 µg ml−1 ...
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bioRxiv - Cell Biology 2019Quote: ... 1.2×106 cells were plated into each well of a 6-well plate and transfected with Sns and Eff-1 constructs (200ng each) using Effectene (Qiagen) per the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... we transfected S2 cells in each well of a 6-well plate with 1 µg each of HA/GFP-tagged constructs using Effectene (Qiagen). After incubation for 3 days ...
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bioRxiv - Genetics 2019Quote: ... for the PCR analysis of potential recombination events genomic DNA from 1×10^6 cells was extracted using DNeasy Blood & Tissue Kits (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 1× PBS and total DNA was extracted at 6 hpi with DNeasy Blood/Tissue DNA mini kit (Qiagen) and quantitated by a nanodrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... Cells were stimulated with LPS (1 μg/ml) for 6 hours at 37°C followed by RNA isolation using a RNeasy Kit (QIAGEN). Extracted RNA was converted to cDNA with an iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... 6 µl Hi-Perfect transfection reagent (Qiagen, 301707), and 1 µl of the desired (20 µM ...
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bioRxiv - Biophysics 2021Quote: ... and the bindingPCA plasmid containing the ligand GAB2 or CRIPT fused to DHFR1,2 (pGJJ006 and pGJJ066 respectively) were digested with the same enzymes and purified using the QIAquick Gel Extraction Kit (QIAGEN).
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bioRxiv - Cell Biology 2020Quote: ... RNA was further digested with TURBO DNase overnight at 37°C (10 U per 2 μg of RNA) and purified with the RNeasy MinElute Cleanup Kit (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... TRAF3 KO1 and TRAF3/p100 double KO cells from 2 experimental repeats was isolated using the RNeasy Mini Kit (Qiagen). RNA quality control was performed using a 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Immunology 2021Quote: ... The pellets were resuspended with OMV buffer and re-pelleted again by centrifugation at 200,000 g for 2 h at 4°C and lysed with Qiazol followed by RNA isolation with the miRNeasy kit (Qiagen) to obtain total RNA including the small RNA fraction ...
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bioRxiv - Genetics 2020Quote: ... in a 2 ml Eppendorf (Hamburg, Germany) tube using Stainless Steel Beads (5 mm) and the TissueLyzer (QIAGEN, Venlo, Netherlands). After an incubation time of 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... run on a 2% agarose gel and a band of 300bp approximately was cut and gel purified using QIAquick Gel Extraction Kit (QIAGEN). Library concentration was determined with KAPA Library Quantification Kit for Illumina Platforms (Kapa Biosystems) ...
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bioRxiv - Immunology 2022Quote: ... the DNA was extracted from 100 mg mouse fecal samples (9-week-old, 2 weeks after final oral gavage treatment) using QIAamp PowerFecal Pro DNA kit (Qiagen) following the manufacturer’s instruction ...
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bioRxiv - Biophysics 2022Quote: ... Purified protein was diluted in PBS buffer (pH = 7.2) and the fluorescence intensity was recorded at 60 °C in the Rotor-Gene 6600 real-time PCR cycler (Qiagen) for 18 h ...
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bioRxiv - Genomics 2020Quote: ... total RNA was extracted from cells grown in five different salinity treatments (0, 2, 12, 24, 36 parts per thousand) using the RNeasy Plant Kit (Qiagen), and 15 Illumina libraries were prepared using the Kapa mRNA HyperPrep kit and sequenced on the Illumina HiSeq2000 platform at the Beijing Genomics Institute.
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bioRxiv - Genomics 2019Quote: ... Purified products were loaded onto a 2% E-gel and gel extracted using a QiaQuick Gel Extraction kit (Qiagen 28704). The molarity of the gel-extracted PCR product was quantified using KAPA library quant (KK4824 ...
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bioRxiv - Genomics 2020Quote: The commonly up- or downregulated genes in cluster 1 and 2 compared to cluster 0 of untreated MDA-MB-231 cells were subjected to the Ingenuity Pathway Analysis (IPA) (QIAGEN) (Kramer ...
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bioRxiv - Cancer Biology 2019Quote: Mouse muscles was pulverized in liquid nitrogen and homogenized 2 × 0.5 minutes at 30 Hz using a TissueLyser II bead mill (Qiagen, USA) in ice-cold homogenization buffer ...
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bioRxiv - Genetics 2019Quote: ... purified using an E-gel electrophoresis system (Agarose 2%) followed by column purification with a MinElute PCR Purification Kit (Qiagen). In order to introduce the doped sequence in the full-length TDP-43 sequence the purified oligonucleotide was cloned into 100 ug of linearized pRS416 Gal TDP-43 by a Gibson approach (Supplementary Table 1 ...
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bioRxiv - Microbiology 2019Quote: ... medium at 37°C and was made competent with rubidium chloride according to the method provided in the QIAexpressionist manual protocol 2 (Qiagen). When antibiotic selection was required ...
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bioRxiv - Immunology 2020Quote: RNA was extracted from IEC isolations by adding a volume containing 2 x 106 cells in PBS to QIAshredder spin columns (QIAGEN) followed by centrifugation at 9,300 g for 1 min ...
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bioRxiv - Systems Biology 2020Quote: ... Cells in cold methanol were transferred to 2 ml polypropylene tubes and metabolite extraction was carried out with a TissueLyser II (Qiagen), using tube holders pre-cooled at -20 °C and adding the same volume of acid-washed glass beads (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted from 2 mL of overnight growth in BHI broth using the DNeasy Blood and Tissue kit (Qiagen). Genome sequencing of the two isolates was performed on a Miseq platform using Illumina technology ...