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Citations for Qiagen :
651 - 700 of 835 citations for Recombinant Mouse Map2k1 His & GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... Mouse C2C12 myotubes: Total RNA was extracted using TRIzol and purified using RNeasy Mini spin columns (QIAGEN). Reverse transcription was performed using a High-Capacity cDNA Reverse Transcription kit (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... Two mouse and two human stool samples were homogenized using a PowerLyzer 24 Homogenizer (110/220V; Qiagen). DNA was extracted using four different bead beating times ...
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bioRxiv - Neuroscience 2022Quote: RNA purification from both IPSC and primary mouse cortical cultures were done using RNeasy 96 Kit (Qiagen) according to the manufacturer protocol ...
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bioRxiv - Pathology 2022Quote: ... and total RNA of mouse mesenteric fat was isolated using the RNeasy Lipid Tissue Mini Kit (Qiagen). The isolated RNA was transcribed to complementary DNA via Superscript IV VILO Master Mix (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from transfected 293T cells and frozen mouse testes using RNeasy Mini kit (QIAGEN). Extracted RNA was used for cDNA synthesis using iScript cDNA Synthesis kit (BIO-RAD ...
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bioRxiv - Biochemistry 2023Quote: Genomic DNA (i.e., gDNA) was isolated from mouse liver tissue using the DNeasy Blood & Tissue Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: RNA was isolated from one quarter of a mouse kidney using RNeasy Plus Mini Kit (Qiagen, 74134). For quantitative real-time PCR ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted from mouse cecal contents and in vitro cultures using the RNeasy PowerMicrobiome Kit (Qiagen). Ribosomal RNA depletion was performed using the RiboMinus™ Transcriptome Isolation Kit (Invitrogen) ...
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bioRxiv - Physiology 2024Quote: Total RNA from mouse hearts (normal, ischemic, or non-ischemic) was isolated using RNeasy Mini kits (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA extraction was done on mouse kidney lysate using the RNeasy Plus Universal Midi kit (Qiagen). RNase digestion was then performed on the RNA extract using the following RNases ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from de-identified tubes of mouse tissues using the RNeasy Kit (QIAGEN; Germany), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and the pellet was lysed using RLT buffer (mouse cells) or QIAZol (human cells) (Qiagen, Cat. 79306). RNA was extracted using the Qiagen RNeasy micro kit.
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bioRxiv - Microbiology 2023Quote: Mouse cDNA was amplified with gene-specific primers (see below) and a HotStarTaq Master Mix Kit (Qiagen). Amplicons were cloned and inserted into a pGEM-T vector (Promega ...
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bioRxiv - Neuroscience 2023Quote: Mouse primary hippocampal neurons grown on coverslips were transiently transfected at DIV11 with Alexa 488-labeled siRNA either specifically targeting mouse Kcc2b (Mm_Slc12a5_1 FlexiTube siRNA, GeneGlobe ID: SI01419243, GenBank accession no: NM_020333; Qiagen) or Ank3 mRNA (Mm_Ank3_3 FlexiTube siRNA ...
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bioRxiv - Cell Biology 2023Quote: ... and reads were aligned to the mouse reference genome Refseq GRCm39.105 from the Biomedical Genomics Analysis Plugin 20.0.1 (Qiagen). Normalization of RNA-seq data was performed using trimmed mean of M-values ...
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bioRxiv - Microbiology 2024Quote: Eukaryotic RNA was isolated from mouse tissue samples (stomach and colon) stored in RNAprotect Tissue Reagent (Qiagen) at ™70 °C before extraction using a GeneJET RNA Purification Kit (Cat ...
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bioRxiv - Genomics 2024Quote: RNA was extracted from mouse tissue samples using an AllPrep DNA/RNA Mini Kit (Qiagen, Valencia, CA). Homogenization buffer for RNA purification was made by adding 1:100 beta-mercaptoethanol to Buffer RLT (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: Mouse-derived xenograft HCC tumors were homogenized in RIPA buffer using metal beads and the LT TissueLyser (Qiagen). Samples were sonicated (Branson Sonifier 3310s ultrasound probe ...
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bioRxiv - Microbiology 2022Quote: Total genomic DNA of mouse fecal samples was extracted using QIAamp PowerFecal Pro DNA Kit (Qiagen, CAT#: 51804), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Plcg2 knockdown was achieved in M-MØP cells using an antisense LNA GapmeR produced against mouse Plcg2 (Qiagen). Cells were grown to 80% confluence (∼2.4 × 105 cells per well ...
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bioRxiv - Cancer Biology 2020Quote: Genomic DNA from mouse bone marrow cells was extracted by the DNeasy Blood and Tissue Kit (Qiagen, #69504). The DNA region containing sgRNA target sites was amplified by PCR using specific primers (Zrsr2 Forward ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from 10 µL of mouse sera using QIAamp® viral RNA Mini Kit (Qiagen) or about 20 mg of organs homogenised by Precellys Lysing Kit Hard tissue grinding (Bertin Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Cell-free cfDNA (both human and mouse) was isolated with QIAamp DSP Circulation NA Kit (QIAGEN, Hilden, Germany) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Nucleic acids were extracted from mouse fecal samples and inoculum samples using the DNeasy Powersoil HTP Kit (QIAGEN) and from the further simplified SC2 samples using the Powermag Microbiome kit (MoBio) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: DNA from mouse heart ventricular tissues or NRVMs was extracted using QIAamp® DNA Mini Kit (Qiagen, 51304) according to the manufacture’s procotol ...
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bioRxiv - Microbiology 2022Quote: ... The cDNA was analyzed with the Qiagen RT2 Mouse Cytokines and Chemokines array (PAMM-150Z, Qiagen, Hilden, Germany). Reactions were run on a QuantStudio 5 Real-Time PCR Instrument (A28133 ...
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bioRxiv - Neuroscience 2021Quote: The total RNA from mouse cortex and human PBMC was extracted using the RNeasy® Mini Kit (Qiagen) and real-time PCR was done as described in the Supplementary material.
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bioRxiv - Neuroscience 2020Quote: Total RNA was extracted from cells or human or mouse brain tissues using RNeasy Mini kit (Qiagen, 74106). Reverse transcription was carried out using iScript Reverse Transcription Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: Viral RNA was isolated from 30 µl of mouse sera using the QIAmp Viral RNA Mini Kit (Qiagen) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were washed with PBS-T (PBS1x/0.1% Tween 20) and incubated with mouse antibody against 6xHis-tag (1:2,000; Qiagen), rabbit antibody against GST-tag (1:10,000 ...
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bioRxiv - Cell Biology 2019Quote: ... Chambers were washed with BRB80 and then were coated with mouse antibody against 6xHis-tag (1:50; Qiagen) for 10 min ...
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bioRxiv - Biochemistry 2021Quote: Total RNA was extracted from HKC-8 or mouse kidneys using the miRNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Xenograft and HCC mouse samples were homogenized in RIPA buffer using metal beads and the LT tissuelyzer (Qiagen). All samples were sonicated (ultrasound probe ...
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bioRxiv - Microbiology 2021Quote: Mouse lung tissues were homogenized in 1 ml of MEM-free media using TissueRuptor (Qiagen, Chadstone, Victoria, Australia). The homogenate was obtained by centrifugation at 6,000 × g for 5 min at 4℃ ...
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bioRxiv - Neuroscience 2021Quote: Microdissected mouse and human brain tissue was flash frozen and homogenized in Trizol using a TissueLyser II (Qiagen). Phenol chloroform extraction was then used together with the RNeasy mini kit (QIAGEN ...
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bioRxiv - Physiology 2022Quote: Total RNA was extracted from the podocytes or mouse glomeruli using the RNeasy Plus Mini Kit (Qiagen, #74134). 1 μg of RNA was reverse transcribed using Oligo dT primers and a high-capacity cDNA Synthesis Kit according to the manufacturer’s instructions (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... mouse and macaque retina or RPE/choroid tissue were isolated using the AllPrep DNA/RNA Mini Kit (Qiagen). Briefly ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from mouse cecal contents or feces using the QIAmp PowerFecal DNA kit (QIAGEN, CA, USA). A multiplexed amplicon library converting the 16S rDNA V4 region was generated from the DNA samples ...
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bioRxiv - Systems Biology 2023Quote: ... RNAs were extracted from isolated mouse LV or SAN with RNeasy Mini Kit (Qiagen, Valencia, CA, United States) and DNAse ...
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bioRxiv - Molecular Biology 2023Quote: We also used the RT2 Profiler PCR array mouse fibrosis (cat. #PAMM 120-ZE-4) kit from Qiagen to detect the expression of 84 fibrosis-related genes ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was isolated from a KI mouse brain hemisphere using the RNeasy Plus Universal Mini Kit (Qiagen, 73404), 2 µg of RNA was reverse-transcribed using the Transcriptor First Strand cDNA Synthesis Kit with random hexamer primers (Roche ...
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bioRxiv - Cancer Biology 2023Quote: Approximately 1 cm of the mouse distal ileum was cut into small pieces and stored in RNAlater (Qiagen). RNA was prepared using a combination of Trizol (Ambion ...
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bioRxiv - Neuroscience 2023Quote: ... PCR was conducted with the Mouse Inflammasomes (96-well format) RT2 Profiler PCR Array (Cat. # PAMM-097Z, Qiagen) according to the manufacturers protocol and was read on an Applied Biosystems® ViiA 7 Real-Time PCR System and analysis was conducted on QuantstudioTM Software by Applied Biosystems ...
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bioRxiv - Physiology 2024Quote: Bulk RNA was isolated from mouse hearts (normal, ischemic, or non-ischemic) using the miRNeasy Mini Kit (Qiagen). Total RNA samples (100-500 ng ...
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bioRxiv - Cancer Biology 2023Quote: The RNA was isolated from cultured cell lines or mouse tissues using the RNeasy Mini Kit from QIAGEN. This kit allows for efficient isolation of high-quality RNA from various sources.
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from mouse LV using the Qiagen RNeasy Mini Kit for mRNA analyses (Qiagen, 74106). List of oligos is accessible and details can be found in the Supplemental Material.
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bioRxiv - Microbiology 2023Quote: ... The cDNA was analyzed with the Qiagen RT2 Mouse Cytokines and Chemokines array (PAMM-150Z, Qiagen, Hilden, Germany). Reactions were run on a QuantStudio 5 Real-Time PCR Instrument (A28133 ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from primary mouse endothelial cells using a commercially available kit (Qiagen, Valencia, CA, USA) followed by DNase I treatment ...
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bioRxiv - Genomics 2024Quote: Genomic DNA was extracted from PGK12.1 mouse embryonic stem cells using the DNeasy blood and tissue kit (Qiagen), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: We isolated RNA from single mouse embryonic hearts at embryonic stage E12.5 by using the RNeasy Kit (#74104; Qiagen) and the RNase-Free DNase Set (#79254 ...