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Citations for Qiagen :
651 - 700 of 2127 citations for PD 1 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... 4 – 4.5 week-old neurons co-cultured with mouse astrocytes were lysed and total RNA was extracted using RNeasy mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... were premixed and added to each well of RT² Profiler™ PCR Array Mouse NFκB Signaling Pathway (Qiagen, PAMM-025Z) for target gene qPCR ...
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bioRxiv - Immunology 2021Quote: ... Real-time quantitative RT-PCR analysis of the samples was carried out using the mouse antiviral response RT2 Profiler PCR array (Qiagen) on a 7900HT Fast Real-Time PCR system (Thermo Fisher ...
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bioRxiv - Cancer Biology 2021Quote: ... gDNA was isolated from at oncogene-negative mouse cell lines or FACS-sorted Tomatopositive cancer cells using either the AllPrep DNA/RNA(Qiagen) or the DNeasy Blood and Tissue Kit ...
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bioRxiv - Neuroscience 2020Quote: ... PCR amplicons of exon 5 of the mouse Nprl3 gene were purified using a PCR purifications Kit (Qiagen, Hilden, Germany) and sent to the Massachusetts General Hospital Center for Computational and Integrative Biology DNA Core for whole amplicon next-generation sequencing (NGS ...
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bioRxiv - Genomics 2021Quote: ... For analysis of Pax8-CreERT2 insert high molecular DNA was prepared from mouse ears using MagAttract HMW DNA Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: Bacterial DNA was extracted from sorted and unsorted mouse fecal-derived bacterial cells using the AllPrep PowerFecal DNA/RNA kit (Qiagen) as per manufacturer instructions ...
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The MuSK-BMP pathway maintains myofiber size in slow muscle through regulation of Akt-mTOR signalingbioRxiv - Cell Biology 2022Quote: Total RNA from WT and ΔIg3-MuSK mouse soleus and TA was extracted using a RNeasy Fibrous Tissue mini kit (Qiagen) for non-stranded RNA library preparation and sequencing (Genewiz).
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bioRxiv - Immunology 2022Quote: ... Three hundred cells from three to six mouse per condition were sorted directly into 25 μl TCL buffer (Qiagen, 1031576) supplemented with 1%β-mercaptoethanol at single cell precision using a BD FACS Aria II instrument ...
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bioRxiv - Microbiology 2022Quote: Bacterial RNA from mouse GI contents (approximately 250 mg) was extracted using RNeasy® PowerMicrobiome® kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Microglia cultures from mouse pup spinal cords were lysed for total RNA extractions using RNeasy Plus Mini Kit (74134, Qiagen). cDNA generation was performed using High-Capacity cDNA Reverse Transcription Kit (4368814 ...
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bioRxiv - Neuroscience 2023Quote: RNA from human microglia differentiated in vitro and human microglia extracted from human-mouse chimeras was extracted using the RNeasy Plus Micro Kit (Qiagen). The RNA from 3 independent in vitro microglia differentiations per cell line were pooled at equal weight to obtain six samples ...
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bioRxiv - Neuroscience 2023Quote: Total RNA from the mouse whole hypothalamus and epididymal white adipose tissue were extracted using the RNeasy Universal Plus Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... for aliquoting into a 384-well RT2 Profiler PCR Array with genes from the Mouse Inflammatory Response and Autoimmunity set (Qiagen). Plates were analyzed with the Applied Biosystems™ QuantStudio™ 6 Flex Real-Time PCR System ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA of E8.5-E9.5 mouse embryos and HFF-hiPS cells (used as a positive control) were extracted using DNeasy Blood and Tissue Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Samples were enriched for CD45+ cells using an EasySep Mouse TIL (CD45) Positive Selection kit (STEMCELL) and RNA was extracted with an RNeasy Plus Mini Kit (Qiagen). TdLN samples were processed as previously described ...
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bioRxiv - Immunology 2023Quote: ... Samples were again enriched for CD45+ cells using an EasySep Mouse CD45 Positive Selection kit (STEMCELL) and RNA was extracted with an RNeasy Plus Mini Kit (Qiagen). RNA was stored at −80°C until further processing.
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was prepared from mouse and rat embryonic molar tooth RNA (extracted using RNeasy Plus Micro kit, Qiagen, Düsseldorf, Germany). cDNA constructs were inserted in TOPO II PCR-plasmids and using TOPO TA Cloning kit with chemically competent cells according to manufacturer’s protocol (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: Genomic DNA from 24 months old CreER and nmrHAS2 mouse livers was purified using a DNeasy Blood & Tissue Kit (Qiagen). One hundred nanograms of purified genomic DNA were used for the methylation measurement ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA from mouse embryos were isolated using RNeasy plus micro kit with on-column DNase I digestion method (QIAGEN). cDNA libraries were produced by using an Ovation RNAseq amplification kit Ver ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell-free DNA from cell culture supernatants and mouse plasma was manually isolated using the QIAamp MinElute ccfDNA Mini Kit (Qiagen), while cfDNA from patient plasma samples was isolated with QIAsymphony SP using the QIAsymphony DSP Circulating DNA Kit (Qiagen) ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: qRT-PCR was performed on total RNA extracted from adult mouse tissues or organoid cultures using the RNeasy Mini Kit (Qiagen). A DNAse I treatment (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA from the human cell lines and mouse tumor samples was extracted using RNeasy Plus Mini Kits (Qiagen, 74134) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from mouse brains with TRIzol or from selected CD11b or CD4-positive cells with RNeasy Plus Mini Kit (Qiagen) and reverse-transcribed ...
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bioRxiv - Neuroscience 2023Quote: Total RNA from the developing mouse cerebral cortex and isolated GFP-positive cells were prepared using the RNeasy mini kit (Qiagen). cDNA was synthesized using SuperScript III reverse transcriptase (Thermo) ...
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bioRxiv - Neuroscience 2023Quote: Total RNA from mouse cortical tissues and primary cortical neurons were isolated using QIAshredder and RNeasy Plus Mini Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: One frozen liver piece of 25mg per mouse was homogenized in a closed tube with glass beads and 1mL Qiazol by using the Tissue Lyser (Qiagen) for 5min at 50Hz ...
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bioRxiv - Immunology 2024Quote: ... Ribosomal RNA was depleted from the total RNA using QIAseq fast select multi-RNA removal kit for mouse RNA(Qiagen) and then RNAseq libraries were made using NEB Next Ultra II Directional RNA library prep kit for Illumina ...
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bioRxiv - Genomics 2020Quote: ... purified nucleic acids were treated with 1-5 μl 1 mg/ml RNaseA (Qiagen) for 45 minutes at 37 °C to degrade RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... and 30 uL/well 1 X lysis buffer (1x Qiagen TCL, 1% beta-mercaptoethanol) was added ...
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bioRxiv - Biophysics 2022Quote: ... The YB-1 (1-180) protein fragment was purified following the manufacturer’s recommendations (Qiagen). Briefly ...
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bioRxiv - Biophysics 2022Quote: ... supernatant was combined with Ni-NTA resin (1 mL/1 L of biomass, Qiagen) pre-equilibrated with 20 mM HEPES ...
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bioRxiv - Genomics 2023Quote: ... slides were treated with 1 mg/ml RNase A (1 mg/ml, Qiagen, 19101) in 2x SSC for at least 45 min at 37°C and then dehydrated in a 70% ...
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bioRxiv - Molecular Biology 2021Quote: ... 1× QuantiNova RT Mix (Qiagen), 1× QuantiNova SYBR Green RT-PCR Master mix ...
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bioRxiv - Microbiology 2021Quote: ... Strep 1:1000 (Qiagen, 34850).
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bioRxiv - Evolutionary Biology 2022Quote: ... 1 μM oligo-dT18 (Qiagen) and 10 μM random hexamers (Promega).
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bioRxiv - Epidemiology 2019Quote: ... version 1 (Qiagen, Hilden, Germany) with the automated instrument QIAsimphony (Qiagen ...
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bioRxiv - Biochemistry 2021Quote: ... 1% SDS (Qiagen P2 buffer). Neutralize lysis buffer by adding 300 μL of 4.2 M Guanidine HCl ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μl of DNase (Qiagen) was added ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of QIAzol (Qiagen) was added to each pellet suspension before being transferred to Lysing Matrix B tubes (MP Biomedicals) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 U Hotstar Taq (Qiagen), 1X Hotstar Taq buffer ...
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bioRxiv - Biochemistry 2023Quote: ... 1% SDS (Qiagen P2 buffer). Neutralize lysis buffer by adding 300 μL of 4.2 M Guanidine HCl ...
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bioRxiv - Microbiology 2023Quote: ... and 1 μM TSO (Qiagen). The master mix was dispensed using the MANTIS liquid dispenser followed by mixing for 1 min at 1800 rpm on a plate shaker (Biosan) ...
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bioRxiv - Bioengineering 2020Quote: ... A Qiagen RT2 SYBR Green master mix with validated qPCR human primers (for HUVECs and BeWo) or mouse primers (for N27 cells) from Qiagen (Frederick) were used to determine relative magnitudes of gene-expression levels using RT-PCR ...
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bioRxiv - Molecular Biology 2021Quote: Total cellular RNA was extracted from mouse placental tissue or HTR cells with an RNeasy Plus Mini Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: LINE1 and SINEB1 probes were similarly generated except using mouse genomic DNA template extracted from E14 mESCs with DNeasy Blood & Tissue Kits (Qiagen 69504) for PCR ...
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bioRxiv - Cell Biology 2021Quote: Whole DNA was extracted from 15-25 mg of mouse tissue using the DNeasy Blood and Tissue Kit (QIAGEN, cat# 69504) according to the kit’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted from mouse retinas using the RNeasy Mini and Micro Kits (Cat No: 74104, 74004, Qiagen, Hilden, Germany). RNA was reverse transcribed with the SuperScript IV First-Strand Synthesis System (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: RNA samples from WT and Rlip+/- mouse liver and brain were prepared using the RNeasy mini kit from Qiagen (Valencia, CA) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated from cell lines or kidney and liver of mouse using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...