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Citations for Qiagen :
651 - 700 of 1879 citations for P N Nonylphenol Diethoxylate Unlabeled 500 Ug Ml In Acetonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Blood was collected by cardiac puncture and harvested organs were homogenized in 500 μl PBS with two 5 mm stainless steel beads using a tissue lyser (Tissue-Lyser II, Qiagen) for two 2-minute rounds at 30 Hz and centrifuged to pellet debris for 10 minutes at 8,000 rpm ...
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bioRxiv - Microbiology 2020Quote: ... A total of 25 cycles of PCR was performed and the expected PCR products (500-600 bp) were gel purified (Qiagen). NGS was performed on the Ion S5 GeneStudio platform ...
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... Isolated genomic DNA (500 ng) of E8.5 mouse embryos (QIAamp DNA mini kit, 51304 or AllPrep DNA/RNA Micro Kit, Qiagen, 82084) and spike-in lambda DNA (1.25 ng ...
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bioRxiv - Pathology 2022Quote: ... and 500 ng to 1 μg of DNA was treated with bisulfite using an EpiTect DNA bisulfite kit (QIAGEN, Germany) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2019Quote: ... Undigested debris were spun down 3 min 10000 rpm and the supernatant was supplemented with 500 μl of 100% ethanol prior loading onto RNeasy mini columns (Qiagen). From this step onwards ...
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bioRxiv - Genetics 2019Quote: ... DNA was size fractionated on 1% agarose gels and 500-750 bp DNA fragments were gel extracted with the Qiaquick gel extraction kit (Qiagen). DNA was PCR amplified for 12 cycles with Illumina primers PE1.0 and PE2.0 and Phusion DNA polymerase (1 unit ...
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bioRxiv - Microbiology 2019Quote: ... A total of 25 cycles of PCR was performed and the expected PCR products (500- 600 bp) were gel purified (Qiagen). NGS was performed on the Ion S5 GeneStudio system ...
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bioRxiv - Cell Biology 2020Quote: HCT8 cells were treated with MakA (500 nM) for 48 hours and total RNA was extracted using RNeasy Mini Kit (Qiagen). Library preparation and sequencing was completed by Novogene (China ...
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bioRxiv - Genomics 2020Quote: ... The reaction was incubated at 37 °C for 30 min in a thermomixer with shaking at 500 rpm and the tagmentated DNA was isolated with MinElute PCR Purification kit (Qiagen). PCR was performed using NEBNext High-Fidelity 2X PCR master mix (72°C 5min ...
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bioRxiv - Microbiology 2019Quote: ... Gel was excised ranging from 300-500 bp and DNA was extracted using QIAquick Gel Extraction Kit (Qiagen, Valencia, CA). The purity and concentration of DNA were measured using Qubit 2.0 Fluorometer ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... we treated 500 ng of RNA with DNase (Perfecta; QuantaBio) then performed cDNA reverse transcription using the QuantiTect Reverse Transcription Kit (Qiagen). We then ran real-time qPCR reactions with SYBR Green detection chemistry using the following reaction mix ...
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bioRxiv - Cell Biology 2021Quote: ... These M-cells were resuspended in 500 μl PBS pH 7.2 and mixed with 7 μl BioMag magnetic streptavidin beads (Qiagen, 311714) for 60 min at 4°C and loaded into an acrylic column with curved grade N52 magnet ...
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bioRxiv - Genetics 2021Quote: ... Specific primers were designed to cover approximately 500 base pairs around the mutations and the regions were PCR-amplified using Qiagen Multiplex PCR kit (Qiagen). The PCR products were sequenced with corresponding primers using canonical Sanger sequencing at SeqLab-Microsynth (Göttingen ...
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bioRxiv - Microbiology 2020Quote: ... DNA and RNA was extracted from the sediment core samples (∼500 µl) and microcosm samples (∼250 µl) using the RNeasy PowerSoil Total RNA Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... 5 μl of amplified cDNA from each well of a 96-well plate were pooled and completed to 500 μl with PCR-grade H2O (Qiagen). Two rounds of 0.6X solid-phase reversible immobilization beads (AmpureXP ...
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bioRxiv - Immunology 2020Quote: ... The resulting ~500 bp long PCR product was subjected to electrophoresis in agarose gels and purified using Gel Extraction Kit (Qiagen). The samples were mixed in equimolar ratios to obtain 30 μl of a final concentration of 10-nM and sequenced by Illumina MiSeq (v3 kit ...
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bioRxiv - Genetics 2019Quote: ... DNA was size fractionated on 1% agarose gels and 500-750 bp DNA fragments were gel extracted with the Qiaquick gel extraction kit (Qiagen). A first round of PCR was performed using primers GSP1 and P1 (First denaturation step ...
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bioRxiv - Developmental Biology 2022Quote: Cells were pelleted by centrifugation after isolation (500 g for 10 min) and subsequently lysed for RNA isolation using RNeasy Micro Kit (Qiagen). For some assays ...
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bioRxiv - Neuroscience 2023Quote: ... freshly sorted cells were pelleted at 500 g for 10 minutes at 4° and then resuspended in RLT Plus buffer (Qiagen). Cells were vortexed and frozen for at least one day at −80° before being thawed on ice and processed for RNA using an RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Genomics 2023Quote: ... Run time was set to 10 minutes and DNA between 150 and 500 bp was excised from the gel and purified using the QIAquick Gel Extraction Kit (Cat. #28706X4, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplified cDNA was subsequently isolated by separation in a 3% agarose gel and isolation of cDNAs 200 to 600 base pairs in length (corresponding to 100 to 500 nucleotide RNAs plus adaptors) using QIAquick gel extraction kit (Qiagen) according to manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2022Quote: Retinal organoids co-cultured with MPCs were collected in 500 μL of Trizol and total RNA was isolated using a RNeasy micro kit (QIAGEN) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Libraries were prepared using Kapa stranded mRNA Hyper Prep sample preparation kits from 500 ng of total RNA isolated using RNeasy columns (Qiagen). The finished dsDNA libraries were quantified by Qubit fluorometer (Thermo Fisher) ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted from 500 μL aliquots of the cell suspension from each culture using a DNA Blood & Tissue Kit (Qiagen) following the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... calf muscle and pancreas were collected in 500 μl of PBS containing one (heart and pancreas) or two (calf muscle) 5 mm stainless steel beads (QIAGEN), homogenized with TissueLyser II (QIAGEN ...
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bioRxiv - Neuroscience 2023Quote: Monolayer of 1.0 × 106 cells grown in 6 well plate was lysed in 500 μl/well QIAzol Lysis Reagent (Qiagen #79306) and RNA extraction was performed with QIAzol miRNeasy Mini Kit (Qiagen #217004 ...
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bioRxiv - Genomics 2024Quote: ... Poly(A)RNA (500 ng) from each independent transfection was reverse transcribed to cDNA using QuantiTech Reverse Transcription kit (Qiagen) using a reporter-specific first strand primer (HJ7211 ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 5 °C at 18 500 rpm for 30 min and loaded to gravity flow Ni-NTA agarose resin (QIAGEN) previously equilibrated with wash buffer WB2_1 (50 mM Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2024Quote: ... one fecal pellet per procedure was placed in a 2 mL round-bottom tube containing a 0.5 cm diameter stainless steel bead and 500 µL of sterile phosphate-buffered saline (PBS) and agitated at 1/30s using a TissueLyser II (Qiagen) for 60 seconds ...
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bioRxiv - Molecular Biology 2024Quote: ... and the supernatant was added to a 500 μl equilibrated Glutathione Sepharose 4 Fast Flow affinity medium (GE) or Ni-NTA agarose (Qiagen). The lysate was incubated with an affinity matrix for 1 hour at 4 ⁰C ...
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bioRxiv - Plant Biology 2022Quote: ... DNA was resuspended in 30 μL of mQ water and incubated with 1 μL of RNase (10 mg/mL prepared from a stock of 100 mg/mL of RNase A from Qiagen, Darmstadt, Germany) at 37 °C for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining lysate was then transferred to 15 ml tubes containing 5 ml lysis buffer and 150 μl Ni-NTA agarose beads (Qiagen, Venlo, Netherlands) and incubated for 4 h at room temperature under constant mild agitation ...
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bioRxiv - Microbiology 2023Quote: ... we placed filters in 2 mL tubes with beads and 1 mL TE buffer (Qiagen plasmid isolation kit, Qiagen Germantown, MD, USA) and ran them in the tissue lyser at either ½ speed or max speed for 30 sec or 1 min ...
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bioRxiv - Evolutionary Biology 2020Quote: ... washed with 1 ml RNAprotect® Cell Reagent (Qiagen) and centrifuged for 10 min at 2,500 x g ...
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bioRxiv - Genomics 2020Quote: ... 5 µL Proteinase-K (20 mg ml-1, Qiagen) was added to the solution and incubated at 60°C for 30 minutes ...
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bioRxiv - Genetics 2019Quote: ... or anti-tetra His (0.1 μg/ml Qiagen 34670) were used as primary antibodies ...
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bioRxiv - Microbiology 2020Quote: ... 1 μL of proteinase K (20 mg/mL, Qiagen) was added and samples were incubated at RT for 10 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... gently resuspended in 30 ml G2 lysis buffer (Qiagen), and incubated with 50ug/ml RNaseA (Qiagen ...
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bioRxiv - Biochemistry 2022Quote: ... containing 1,5 ml (CV) of Ni-NTA agarose (Qiagen) previously cleaned and equilibrated with buffer A ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 ml of Matrigel basement membrane matrix (Qiagen #354234) prepared in serum free culture medium (1:8 ratio to reach final concentration of 1.2 mg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... composed of 100 μg/mL RNAse A (Qiagen, 19101) and 20 μg/mL Propidium Iodide (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... 5.0 ml of nickel-agarose (Ni-NTA agarose, Qiagen) was packaged into a 1.5×14 cm column (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... containing 2.116 units/ml QIAGEN protease (Qiagen, Cat#19155). After protease inactivation at 75°C for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... then transferred to a 10 mL disposable column (Qiagen) and washed with an additional 50 mL of WB followed by 100 mL of low salt buffer (LSB ...
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bioRxiv - Genomics 2022Quote: ... 10 mL chilled Qiagen Buffer C1 (from the Qiagen Genomic DNA Buffer Set ...
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bioRxiv - Microbiology 2019Quote: ... resuspended in 1 mL RTL buffer (Qiagen RNeasy Kit) and lysed by bead beating (2 × 1 min ...
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bioRxiv - Biochemistry 2019Quote: ... 3-4 mL of Ni-NTA superflow resin (Qiagen) were loaded onto a column and equilibrated with the resuspension buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... treated with 0.2 mg/mL RNase A (QIAGEN 19101) for 2 hours at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... A 5 ml Strep-tactin Superflow Plus column (Qiagen) was equilibrated with 50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... 30 mL RNA Protect Bacterial Reagent (Qiagen, Hilden, Germany) was added to 15 mL cultures and incubated at 4 °C overnight ...