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Citations for Qiagen :
651 - 700 of 1270 citations for Oropouche Virus Gc Protein Mouse Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The secreted protein in the culture supernatant was purified using Ni-NTA agarose (Qiagen) according to the manufacturer’s protocol and dialyzed against PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were equalized for protein concentration and incubated with NiNTA agarose beads (Qiagen, #L30210) for O/N ...
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bioRxiv - Microbiology 2021Quote: ... Protein was purified using Ni-NTA agarose beads according to the manufacturer’s instructions (Qiagen). A buffer exchange was performed by centrifugation of the protein preparation through an Amicon Ultra-15 centrifugal filter unit (Millipore ...
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bioRxiv - Bioengineering 2020Quote: ... The enriched proteins were searched again Ingenuity pathway analysis (QIAGEN, Redwood City, CA, USA) for their primary subcellular location and enriched molecular functions ...
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bioRxiv - Cell Biology 2022Quote: Constructs for recombinant bacterial protein expression were all cloned in the pQE plasmid (Qiagen). Su9-DHFR and CaM-3C-Alfa-Sec61β(2–60)-OMP25(112–145)F128Amber were cloned downstream of a His14-bdSUMO tag ...
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bioRxiv - Neuroscience 2023Quote: ... significantly differentially expressed proteins from all translatomes were analysed for IPA pathway analysis (Qiagen) to highlight activated/inhibited pathways when comparing control (NBH ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were eluted using Ni-NTA spin columns according to the manufacturer’s protocol (Qiagen). GST-α synuclein fusion protein tagged at the N-terminus was obtained from Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified according to manufacturer’s instructions by Ni2+-NTA agarose beads (QIAGEN). Washing steps were performed with a buffer containing 20 mM Tris ...
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bioRxiv - Cancer Biology 2023Quote: Total cellular RNA was extracted using AllPrep DNA/RNA/Protein mini kit (Qiagen, UK). Reverse transcription was carried out using kits from Invitrogen following the manufacturer’s instructions (SuperScript First-Strand Synthesis System for RT-PCR) ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged proteins were purified by affinity chromatography on Ni-NTA Agarose resin (Qiagen) and eluted with buffer containing 0.5 M imidazole ...
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bioRxiv - Neuroscience 2023Quote: ... and day 7,14,21 and 28 of maturation using AllPrep DNA/RNA/Protein (80004, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: Total protein was extracted from snap frozen tissues with a Tissue Lyser II (Qiagen) using 5mm Bead Beater balls (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was then isolated with Qiagen AllPrep DNA/RNA/Protein Mini Kit (Qiagen # 80004). Breast microstructures from 5 independent donors were exposed to vehicle (PBS and PBS plus DMSO) ...
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bioRxiv - Microbiology 2023Quote: ... The remaining protein mixtures were incubated with 50 μL Ni-NTA agarose beads (QIAGEN) at 4℃ for 1.5 h with constant rotation ...
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bioRxiv - Microbiology 2023Quote: ... the proteins were purified by affinity chromatography with Ni-NTA superflow affinity column (Qiagen) as previously described (33).
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bioRxiv - Cell Biology 2023Quote: ... Proteins harboring the His6 tag were captured by incubation with Ni- NTA resin (Qiagen) (2ml resin per bacterial lysate from 1L culture ...
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bioRxiv - Immunology 2024Quote: ... Proteins were purified from filtered supernatants using nickel nitriloacetic acid (Ni-NTA) agarose (QIAGEN) via gravity flow ...
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bioRxiv - Microbiology 2024Quote: ... Soluble protein fractions were then mixed with pre-equilibrated Ni-NTA agarose resin (Qiagen) and incubated at 4°C for 1 h with rotation ...
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bioRxiv - Microbiology 2023Quote: ... Bacteria isolates then underwent DNA isolation using AllPrep Bacterial DNA/RNA/Protein Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Mouse posterior cortex was disrupted for RNA isolation using the TissueLyser (QIAGEN, Hilden, Germany) for 2 x 2 min at 20 Hz as recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2020Quote: Purified RNA was isolated from mouse skin using the RNeasy Plus Mini Kit (Qiagen) after pulverization of the frozen skin tissue and homogenization with the QIAshredder system (Qiagen) ...
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bioRxiv - Biochemistry 2019Quote: Fecal DNA from mouse colon was extracted by QIAamp PowerFecal DNA Kit (Qiagen, Germany) with minor modifications ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was extracted from mouse hippocampal samples using a RNeasy Mini kit (Qiagen), following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... and from mouse tissue (liver, retina, RPE) using a DNeasy Blood & Tissue kit (Qiagen). Target sequences were PCR-amplified using 2X Taq PCR Smart mix (SolGent) ...
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bioRxiv - Physiology 2020Quote: Whole RNA was extracted from mouse orbital fat samples using RNeasy mini-kits (Qiagen). cDNA was prepared using Superscript III first-strand synthesis kit (ThermoFisher) ...
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bioRxiv - Pathology 2022Quote: Total RNAs were obtained from cells or mouse kidneys using RNeasy Mini Kit (Qiagen) and reverse transcribed using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was then performed with specific primers for mouse IFN-α11 (PPM03050B-200, Qiagen), mouse β-actin (PPM02945B-200 ...
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bioRxiv - Systems Biology 2022Quote: ... DNA was isolated from mouse tissues using the DNeasy Blood and Tissue Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Total RNA from mouse lungs was extracted using RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: Total RNA (100–200 islets/mouse) was extracted using the RNeasy Mini Kit (Qiagen). RNA quality and quantity was measured on the Agilent 2200 TapeStation System ...
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bioRxiv - Molecular Biology 2020Quote: Total mouse heart RNA was isolated with an RNeasy mini kit (Qiagen, Valencia, CA) or RNAqueous® Micro RNA isolation kit (Ambion ...
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bioRxiv - Immunology 2019Quote: ... RT2 Profiler™ PCR Array Mouse Autophagy was purchased from Qiagen (Catalog # PAMM-084Z). RNA extracted from the brain of pups born to infected dams were analyzed for the expression of autophagy-related genes following the manufacturer’s instruction and as previously described (13).
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from mouse MLL-AF9 cells by RNeasy Micro Kit (Qiagen), 72 hours after transduction of the cells ...
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bioRxiv - Molecular Biology 2022Quote: ... for mouse samples and cell lines or with miRNeasy Mini Kit (Qiagen, Hilden, Germany) for human tissue samples ...
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bioRxiv - Neuroscience 2023Quote: RNA from brain organoids and mouse tissue was extracted with RNeasy Mini Kit (Qiagen) for mRNA detection ...
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bioRxiv - Neuroscience 2023Quote: ... DNA was isolated from lentiviral injected mouse brains using the DNeasy kit from Qiagen according to the manufactures protocol with the help of a tissue grinder (Dixon Science) ...
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bioRxiv - Neuroscience 2023Quote: RNA from whole mouse OE samples was extracted using RNeasy kit (Qiagen, Hilden, Germany), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA from mouse blood was extracted using a DNeasy Blood & Tissue Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: RNA was extracted from cells and mouse tumors using the miRNeasy kit (Qiagen, #217004) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... L1-L5 mouse DRG was homogenized with an RNeasy Mini Kit (Qiagen, Valencia, CA) using on-column DNase-I digestion according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... spongiae DNA was extracted from mouse tissues using DNAeasy Blood and Tissue kit (Qiagen). The numbers of mice used in each individual experiment were calculated to permit detection of at least a two- to four-fold difference in bacterial loads between groups with 95% (two-sided ...
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bioRxiv - Microbiology 2024Quote: Mouse cDNA was amplified with gene-specific primers and HotStarTaq Master Mix Kit (Qiagen) (Table 1) ...
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bioRxiv - Molecular Biology 2024Quote: RNA was extracted from mouse islets using the RNAeasy Micro Kit (QIAGEN, Cat # 74004); RNA was extracted from flash-frozen liver and peripancreatic fat tissues using TRIzolTM (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was purified from mouse tissues using the RNeasy Plus Mini Kit (Qiagen) and then transcribed into cDNA using the iScript™ cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Pathology 2024Quote: Mouse kidney outer cortex tissues were dissected and homogenized in QIAzol (QIAGEN, Germantown, MD). Total RNA samples were extracted using RNeasy Plus Universal Kit (QIAGEN ...
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bioRxiv - Microbiology 2019Quote: ... DNA was extracted from each section individually using the protein precipitation method (Puregene, Qiagen, Netherlands).
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bioRxiv - Molecular Biology 2020Quote: ... Protein was extracted using a modification of the Qiagen AllPrep DNA/RNA Mini Kit (Qiagen) according to manufacturer instructions ...
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bioRxiv - Biophysics 2019Quote: ... proteins were purified by batch Ni-NTA bead purification (1 mL slurry/1L culture; Qiagen) and further purified by a Superdex 200 16/60 column (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Uncleaved proteins and TEV were removed by a second pass with Ni-NTA resin (Qiagen). Proteins were concentrated and further purified using gel filtration (S75 16/60 ...
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bioRxiv - Cell Biology 2021Quote: ... CRY2 protein was isolated by Ni2+-nitrilotriacetic acid (Ni-NTA) affinity chromatography (Qiagen, Hilden, Germany) following elution with 50 mM Tris buffer pH 7.5 ...