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Citations for Qiagen :
651 - 700 of 1802 citations for KGF 2 FGF 10 Human 171a.a since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... reaction mixes were prepared with 10 μL QuantiTect Probe PCR master mix (QIAGEN), 1.0 μM primer and 0.25 μM probe ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were transfected with 10 µg plasmid DNA using Effectene transfection reagent (Qiagen). After an additional 24 hours ...
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bioRxiv - Physiology 2023Quote: ... or from 10 dissected fat bodies using the QIAGEN RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... 10 μg dsRNA were transfected into 3×106 Drosophila cells using Effectene (QIAGEN).
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bioRxiv - Biochemistry 2023Quote: ... Filtered cell lysate was applied to 10 mL of Ni-NTA resin (Qiagen) which had been previously equilibrated with 10 column volumes of H2O ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 10 fly heads per condition were homogenized in Qiazol (Qiagen) and total RNA was isolated ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was mixed with 10% (v/v) Ni-NTA agarose beads (Qiagen) preequilibrated with the lysis buffer for 40 min at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... cells were transfected in 10 cm2 at ∼60% confluency using PolyFect (Qiagen, 301107) and washed with complete culture medium 6 h later ...
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bioRxiv - Cell Biology 2021Quote: ... the ViiA 7™ Real-Time PCR system was used to run the Human Stem Cell RT² Profiler™ PCR Array (Qiagen, Hilden, Germany). For the analysis of cardiac differentiation-associated genes ...
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bioRxiv - Cancer Biology 2021Quote: ... After 2 hours we extracted the total RNA (RNeasy mini kit, Qiagen, cat. n. 74104) from 40 animals for each of the 3 experimental replicates (total ...
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bioRxiv - Cell Biology 2020Quote: RNA from 2×107 cells was isolated using the RNeasy Mini Kit (Qiagen, Venlo, Netherlands), cDNA was generated by reverse transcription on CFX96 real-time system (BioRad ...
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bioRxiv - Immunology 2022Quote: ... RNA was extracted from 2 μl sucrose purified virus using the RNeasy mini kit (QIAgen). The RNA was then treated with TURBO DNase (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: Total RNA was extracted from 1–2 million cells using the AllPrep Mini kit (QIAGEN) according to the manufacturer’s instructions and 1 μg of total RNA was used to prepare each RNA-seq library ...
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bioRxiv - Cancer Biology 2019Quote: ... and tissue homogenized for 2 min at 30 Hz in a TissueLyser II (Qiagen, NL). cDNA was produced by reverse transcribing 3 µg of RNA using superscript II reverse transcriptase (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by 2 minutes (40 oscillation per second) on a bead shaker (Qiagen Tissue lyser)-centrifuged at 1000g for 5 minutes at 4 °C to remove debris ...
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bioRxiv - Molecular Biology 2020Quote: ... and gonad were collected and stored separately in 2-mL tubes containing RNA later (QIAGEN) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... cfDNA was extracted from 2 ml of plasma using QIAamp Circulating Nucleic Acid Kit (QIAGEN) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was isolated from 2-week-old plant leaves using RNeasy Mini Kit (QIAGEN) Germany ...
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bioRxiv - Microbiology 2019Quote: ... and the supernatant was applied to 2 mL of pre-washed Ni-NTA resin (Qiagen) at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... coli* culture from each growth condition were stabilised in RNAProtect Bacteria Reagent (2 mL; Qiagen) and cells pellets were frozen at −80°C prior to processing ...
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bioRxiv - Developmental Biology 2020Quote: ... for 2 hours after which samples were treated with 5 μL Proteinase K (Qiagen 19131) overnight ...
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bioRxiv - Bioengineering 2021Quote: ... the obtained soluble extract was mixed with 2 ml slurry Ni-NTA beads (30210, QIAGEN), incubated at 4 °C for 2 h with rotation.
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bioRxiv - Biochemistry 2021Quote: ... the obtained soluble extract was mixed with 2 ml slurry Ni-NTA beads (30210, QIAGEN), incubated at 4°C for 2 h with rotation ...
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bioRxiv - Microbiology 2020Quote: RNA was isolated from U-2 OS cells with the RNeasy minikit (Qiagen, Hilden, Germany). 0.5µg RNA was reverse transcribed into cDNA using the PrimeScript RT Reagent Kit (Takara ...
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bioRxiv - Cancer Biology 2020Quote: ... 4 μl of bisDNA was added to 2 μl 10x PCR buffer (Qiagen, Cat# 203203), 2 μl of 10 μM primer mix (Methods Table 1) ...
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AMPK is elevated in human cachectic muscle and prevents cancer-induced metabolic dysfunction in micebioRxiv - Molecular Biology 2022Quote: ... the muscles were homogenized (2 × 30 sec, 30 Hz, TissueLyser II bead mill, Qiagen, USA) in a modified GSK3-buffer (10% glycerol ...
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bioRxiv - Genetics 2022Quote: ... RNA was collected from 2 x 105 pelleted cells using the RNeasy mini kit (Qiagen). qPCR using primers specific for ERAP1 or TNFSF12 were used to validate knockdown relative to 18S rRNA.
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bioRxiv - Biophysics 2022Quote: ... the supernatant was incubated for 2 h at 4 °C with Ni2+-NTA-agarose (Qiagen) (20 mg of proteins/ml of resin ...
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bioRxiv - Microbiology 2021Quote: ... The samples were then homogenized using a Vortex-Genie 2 and vortex adapter (QIAGEN®) at the highest setting for 10 minutes.
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bioRxiv - Genomics 2022Quote: ... Lysate was spun down and supernatant was mixed with 2 ml Ni-NTA resin (Qiagen) per 50 ml sample and incubated for 1h at 4°C ...
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bioRxiv - Immunology 2022Quote: ... BEAS-2B and MRC-5 (2×106 cells/well) using the RNeasy Mini Kit (Qiagen) with DNase I treatment to eliminate DNA contaminants as previously described18 ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 RNA was quantified by RT-qPCR with the QuantiTect Kit (Qiagen, 204443) with Sarbeco E gene primers and probe in a QuantStudio 3 thermocycler (Applied Biosystems) ...
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bioRxiv - Systems Biology 2020Quote: RNA was isolated from 2 x 106 cells with the RNeasy Plus Mini kit (Qiagen) and reversely transcribed with SuperScript III (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... and collected into a PCR tube containing 7 µL of 2×TCL lysis buffer (Qiagen) with 2% v/v 2-mercaptoethanol (Sigma) ...
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bioRxiv - Neuroscience 2019Quote: ... absolute fold change ≥ 2) was performed by Ingenuity Pathway Analysis (IPA) (https://tinyurl.com/y6672c22, Qiagen). Pathways and their associated downstream effects ...
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bioRxiv - Genetics 2021Quote: ... Other ventriculus sinister sections were pestled by a grinding apparatus (Schwingmühle TissueLyser 2, Qiagen, Germany) and stored at −80°C for detection ...
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bioRxiv - Immunology 2021Quote: ... Skin samples were homogenized in a TissueLyser LT (Qiagen, 50 Hz, 2 times 4 minutes) using 5 mm stainless steel beads (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... and 1% penicillin–streptomycin) at 300 Hz for 2 min using a Tissuelyser II (Qiagen) and centrifuged to clarify the supernatant ...
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bioRxiv - Immunology 2022Quote: ... The initial homogenization was performed mechanically (2 metal beads/sample) using a TissueLyserLT (#85600, Qiagen) homogenizer (50 oscillations/s for 10 min) ...
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bioRxiv - Microbiology 2022Quote: ... and 1% penicillin–streptomycin) at 300 Hz for 2 min using a Tissuelyser II (Qiagen) and were then centrifuged to clarify the supernatant ...
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bioRxiv - Microbiology 2023Quote: ... MAYV samples were homogenized at 30 Hz for 2 min in a TissueLyser II (Qiagen) and centrifuged for 30 sec at 11,000 rpm ...
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bioRxiv - Microbiology 2023Quote: ... 1 mL of sample culture was immediately transferred into 2 mL RNA protect bacteria (QIAGEN) and vortexed ...
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bioRxiv - Plant Biology 2023Quote: ... Christ) and ground to powder at 30 Hz for 2 × 45 s (TissueLyzer II, Qiagen). DNA was extracted according to the manufacturers instructions ...
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bioRxiv - Neuroscience 2023Quote: ... PCR primers were designed using the PyroMark Assay Design 2.0 software (Qiagen, Supplementary Table 2). The assay for MBP was tested for its sensitivity using the EpiTect PCR Control DNA Set (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... siRNA-transfection reagent complexes were generated using 2 μL HiPerfect transfection reagent (Qiagen, Germantown, MD) and 25-50 nM siRNA (non-specific control siRNA (sc-37007 ...
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bioRxiv - Microbiology 2023Quote: ... then 50 mg transferred to 2 ml Plant Pro tissue disruption tubes (Qiagen Ltd, U.K.). DNA was extracted following manufacturers protocol ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was run on 2% agarose gel and extracted using QIAquick Gel Extraction Kit (QIAGEN). Fragments and backbone were ligated using T4 DNA ligase (Cat# M0202 ...
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bioRxiv - Neuroscience 2023Quote: Total mRNA from 2 to 4 organoids were isolated using the RNeasy mini kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The 15 µl reactions each contained 2 × reaction mix from the Multiplex PCR Kit (Qiagen), 5 µg Bovine Serum Albumin ...
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bioRxiv - Genomics 2023Quote: ... RNAse A treatment was conducted by adding 2 uL of RNAse A 100ug/mL (Qiagen) and incubating the samples at 37°C for 15 min ...