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Citations for Qiagen :
651 - 700 of 2029 citations for 1 1 Dimethylsila 11 Crown 4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... the precipitates were subjected to Western blot analysis with anti-penta-His (1:2000, Qiagen) and anti-FLAG M2 (1:4000 ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue was ground using a tissue lyser (Qiagen, 3 min at 300 s-1) in 200 µL 500 mM Tris pH 9 by adding a metal ball (diameter 5 mm) ...
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bioRxiv - Genomics 2023Quote: ... total genomic DNA was extracted from 1×106 parasites using DNeasy Blood & Tissue kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: ... cDNA was generated from 1 μg of RNA with the QuantiTect Reverse Transcription Kit (QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... 30 min) and supernatant was loaded onto a 1 ml Ni-NTA Superflow column (Qiagen). Proteins were eluted by an increasing imidazole gradient according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... All samples were subjected to a 1-hour DNAse treatment (Qiagen RNase-free DNase kit), and final elution volume was 20 μl ...
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bioRxiv - Immunology 2024Quote: ... Illumina compatible libraries were generated using the Qiagen Q1ASeq 1-step amplicon kit (Qiagen, #180419), and sequencing was performed on the Illumina MiSeq platform using 2×250 paired end reads with a goal of generating 5,000 – 10,000 reads per sample ...
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bioRxiv - Cell Biology 2023Quote: RNA was isolated from 1×106 MEL cells with the RNeasy Plus kit (Qiagen, 74134). RNA was reverse-transcribed using the Superscript III First-Strand Synthesis System (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Four-cell and morula stage embryos were collected into 1 × TCL buffer (Qiagen, Cat# 1070498) containing 1% 2-mercaptoethanol at 46 and 72 h post-fertilization ...
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: ... Amplified products were resolved on a 1% agarose gel and purified by gel extraction (QIAGEN). Electrocompetent Enterobacter sp ...
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bioRxiv - Microbiology 2023Quote: ... HIV-1 RNA in plasma was extracted using the QIAamp Viral RNA Mini Kit (Qiagen). To amplify the 3’ half viral genome ...
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bioRxiv - Microbiology 2023Quote: ... we placed filters in 2 mL tubes with beads and 1 mL TE buffer (Qiagen plasmid isolation kit ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1 hour and purified by agarose gel extraction (MinElute Gel Extraction Kit, Qiagen #28606). Plasmid constructs were created by Gibson cloning with NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621L ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from 1 × 106 cells using the RNeasy Plus Mini Kit (Qiagen), and cDNA was synthesized with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Biochemistry 2023Quote: Total RNA was extracted from 1 – 2 x 108 cells using the RNeasy kit (Qiagen). DNA was removed using Turbo DNase (Applichem ...
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bioRxiv - Molecular Biology 2022Quote: ... for 1 hour and purified by agarose gel extraction (MinElute Gel Extraction Kit, Qiagen #28606). Gibson cloning was done with NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621L ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA (1 μg) was first reverse-transcribed using the QuantiTect Reverse Transcription kit (Qiagen). 20 ng of total cDNA were then subjected to qRT-PCR using TaqMan Gene Expression Master Mix and pre-designed TaqMan probes (all from Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... Products were extracted from a 1% agarose gel using the QIAquick Gel Extraction Kit (Qiagen). Libraries were pooled and paired-end 150 base pair sequencing reads obtained by sequencing on a HiSeq4000 platform (Novogene) ...
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bioRxiv - Cell Biology 2022Quote: ... Products were extracted from a 1% agarose gel using the QIAquick Gel Extraction Kit (Qiagen). TruSeq sequencing adapters were added to mutant fragments by PCR using HCM1 specific primers fused to the TruSeq universal adapter or unique TruSeq indexed adapters ...
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bioRxiv - Immunology 2022Quote: ... after which the cells were stained with anti-Penta-His-AF647 (1 µg/mL; Qiagen) for 30 minutes at 4°C ...
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bioRxiv - Genetics 2022Quote: ... loaded on a 1% agarose gel and purified using the QIAquick Gel Extraction Kit (Qiagen).
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bioRxiv - Genetics 2022Quote: ... 1 ml of each pooled PCR was purified using the QIAquick PCR Purification Kit (Qiagen), loaded on a 1% agarose gel and purified using the QIAquick Gel Extraction Kit (Qiagen).
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bioRxiv - Microbiology 2022Quote: ... and 1% penicillin–streptomycin) at 300 Hz for 2 min using a Tissuelyser II (Qiagen) and were then centrifuged to clarify the supernatant ...
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bioRxiv - Microbiology 2022Quote: ... Cell pellets were thawed and resuspended in 1 mL QIAzol Lysis Reagent (QIAGEN, Hilden, Germany) and homogenized using a BioSpec Products BeadBeater-24 (Bartlesville ...
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bioRxiv - Microbiology 2022Quote: ... and the entire spleen were harvested and homogenized in 1 ml PBS using TissueRuptor (Qiagen). These homogenates were serially diluted and plated on agar plates to determine the number of colonized bacteria in each organ ...
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bioRxiv - Immunology 2022Quote: ... with β-mercapto-ethanol (1:100) and RNA was extracted using RNeasy micro kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... After chloroform: isoamyl alcohol (24:1) extraction followed by purification (Qiagen RNeasy Mini Kit, 74104), the biotinylated RNA was resuspended in water and biotinylated-RNA was isolated using Dynabeads MyOne Streptavidin C1 magnetic beads (Invitrogen) ...
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bioRxiv - Systems Biology 2023Quote: Approximately 5 mg of frozen prefrontal cortex was homogenized in 1 ml RLT buffer (Qiagen) using a BeadBeater (BioSpec ...
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bioRxiv - Bioengineering 2023Quote: ... and purified from 1% agarose gel using a QIAquick Gel Extraction Kit (Qiagen, Germantown, MD). A double-stranded insert was digested at the same restriction sites ...
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bioRxiv - Evolutionary Biology 2023Quote: ... using a 10:1 ratio of binding buffer (a modified version of Qiagen’s PB buffer) to digestive mixture ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from ∼1 million cells using the RNeasy Mini Kit (Qiagen, #74104) with QIAshdredder Mini Spin Columns (Qiagen ...
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bioRxiv - Plant Biology 2024Quote: ... 1 microgram of total RNA was reverse transcribed using the QuantiTect Reverse Transcription kit (Qiagen). Real-time RT-PCR was performed on 1:10 diluted cDNA using the SensiFAST SYBR No-ROX Kit (Bioline) ...
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bioRxiv - Microbiology 2024Quote: ... and the remaining lysates were incubated with 1 mL of Ni-NTA agarose (Qiagen, 1018244) on nutator (Daeiltech ...
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bioRxiv - Microbiology 2024Quote: ... About 1 A600 unit of culture was harvested and RNA was extracted using RNeasy (Qiagen). cDNA was synthesized after ezDNase treatment using the SuperScript VILO cDNA kit (Thermo Fisher) ...
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bioRxiv - Neuroscience 2024Quote: ... RNA samples were extracted from 1 million cells using the RNeasy Mini Kit (Qiagen, 74136) and treated with TURBO DNA-freeTM Kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 500 µL AP1 lysis buffer and 10 µL proteinase K (10 mg mL-1; Qiagen) were added to each tube ...
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bioRxiv - Immunology 2024Quote: Up to 1 x 105 cells were FACS sorted directly into RLT lysis buffer (Qiagen) supplemented with 14mM β-mercaptoethanol ...
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bioRxiv - Microbiology 2024Quote: Total RNA was extracted from the THP-1 derived macrophages (Qiagen RNeasy Mini Kit, Qiagen) and reverse transcribed to cDNA (High-Capacity cDNA Reverse Transcription Kit ...
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bioRxiv - Microbiology 2024Quote: Total RNA was extracted from the THP-1 derived macrophages (Qiagen RNeasy Mini Kit, Qiagen) and reverse transcribed to cDNA (High-Capacity cDNA Reverse Transcription Kit ...
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bioRxiv - Immunology 2024Quote: ... Homogenization of the tissues was carried out using buffer RLT with 1% β-mercaptoethanol (Qiagen). The aqueous phase was isolated and purified utilizing the RNeasy Plus mini kit (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting cDNA was diluted 1:5 by adding 10 uL of buffer EB (Qiagen) using a Mantis liquid handler ...
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bioRxiv - Genetics 2024Quote: ... plates were shaken 1 min at a frequency of 23 Hz in a TissueLyser (Qiagen), incubated 30 min at 30°C ...
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bioRxiv - Molecular Biology 2020Quote: ... After 14 h binding to 4 mL Ni-NTA agarose beads (Qiagen), the beads were first washed with 15 mL Ni-NTA binding buffer and then with 15 mL Ni-NTA wash buffer (1 M NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... 4 µL of DNA) and run in Rotor-Gene Q machine (QIAGEN). Primer sequences are listed in the table S2 ...
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bioRxiv - Bioengineering 2021Quote: ... Sample supernatants were incubated with 4 mL of Ni-NTA Agarose (Qiagen) for >1 hour at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... and pooled using the UltraClean 96 PCR Cleanup kit (Qiagen 12596-4) and Quant-iT dsDNA High Sensitivity Assay kit (Invitrogen Q33120) ...
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bioRxiv - Microbiology 2022Quote: ... and pooled using the UltraClean 96 PCR Cleanup kit (Qiagen 12596-4) and Quant-iT dsDNA High Sensitivity Assay kit (Invitrogen Q33120) ...
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bioRxiv - Genomics 2022Quote: ... and 4 µl of 100 mg/ml RNase A (QIAGEN, cat# 19101) were added to the homogenate ...
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bioRxiv - Biochemistry 2020Quote: ... Crystallization screening was performed at 4°C with JCSG core I (Qiagen) using the sitting drop vapor diffusion method ...
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bioRxiv - Cell Biology 2020Quote: ... 4 μl of DNA) and run in Rotor-Gene Q machine (Qiagen). Primer sequences are listed in the Table 2 ...