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Citations for Qiagen :
6551 - 6600 of 10000+ citations for Mouse Marginal Zone B And B1 Cell Specific Protein MZB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nup851-564-Seh1-VHH-SAN2 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 1M ammonium sulfate and 0.1 M sodium acetate pH 5.0 (Protein Complex suite, Qiagen). Crystals significantly improved by changing the buffer to di-sodium succinate pH 5.5 and the addition of 4% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were then purified on Ni2+ -NTA affinity resin following a modified version of the manufacturer’s instructions (QIAGEN) [25] ...
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bioRxiv - Microbiology 2020Quote: ... The 6 × his-tagged proteins were purified using a nickel-nitrilotriacetic acid (Ni-NTA) agarose matrix (30250, Qiagen). Cell-free extracts were loaded on 0.5 ml Ni-NTA matrixes and incubated on a roller shaker for 2 h at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Protein lysates were separated on NuPage 5-12% Bis-Tris or 3-8% Tris-Acetate gels (Novex, Qiagen) using MES running buffer (Novex ...
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bioRxiv - Plant Biology 2020Quote: ... The His-tagged proteins were purified by Ni-affinity gravity-flow chromatography using the Ni-NTA agarose (Qiagen) following the product manual.
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bioRxiv - Molecular Biology 2020Quote: ... His-tagged ubiquitin-conjugated proteins were purified by adding 100 µL of 50 % Ni-NTA agarose beads (Qiagen) equilibrated in A2 buffer to 1 mL of cleared extracts and incubated with rotation for 2-4 hr at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... the tags and protease were removed from the protein by passage through a Ni-NTA superflow cartridge (QIAGEN). The protein was further purified by size exclusion chromatography on a Superdex 200 Increase (Cytiva ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein digestion was performed on the eluted DNA samples at 50°C for 30 minutes (Qiagen Protease mix). ChIP DNA was purified using Sera-Mag beads (Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... The lysates were clarified by centrifugation and proteins in the supernatant were purified using Ni-NTA chromatography (Qiagen) for His-tagged proteins or Glutathione Sepharose 4 Fast Flow beads (GE Healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... The protein prokaryotic expression and purification was according to the instruction of Ni-NTA Agarose (Cat.No.30210, QIAGEN) and Glutathione Sepharose (GE).
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bioRxiv - Bioengineering 2020Quote: Initial small test purifications were performed using Ni-NTA Spin Column for His-Tagged proteins (Qiagen, Hilden, Germany) following the protocol for 6xHis-Tagged Proteins under Native Conditions from E ...
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bioRxiv - Biochemistry 2020Quote: ... The Ni-NTA agarose resin for purification of his-tagged proteins was purchased from Qiagen (Germantown, MD, USA). Superdex 75 16/600 and Superdex 200 Increase 10/300 size exclusion chromatography columns were purchased from GE Healthcare (Uppsala ...
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bioRxiv - Immunology 2021Quote: ... to provide Uniprot entries and further processed in Perseus (v1.6.6.1).18–20 Significantly regulated proteins were investigated in Ingenuity Pathway Analysis (v47547484, Qiagen, USA)
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bioRxiv - Developmental Biology 2021Quote: Log fold-change values of the top 5% proteins were used as input for Ingenuity Pathway Analysis (Qiagen: https://digitalinsights.qiagen.com/products/features/) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins NHis6-Fnr1 and Fnr3-CHis6 in the supernatant were purified on Ni2-NTA resin (Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... and the cleaved His6-MBP tag was removed by passing the protein through an amylose resin column (Qiagen). The flow-through fraction containing the tag-free protein was further purified using a Superdex 200 (10/300 GL ...
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bioRxiv - Biophysics 2021Quote: ... and the protein of interest was purified by affinity chromatography on a Ni-NTA column (Qiagen, Hilden, Germany), using a TRIS-HCl 50 mM ...
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bioRxiv - Microbiology 2022Quote: ... filtered and the secreted recombinant proteins were purified by affinity chromatography using Ni-NTA resin (Qiagen; Cat.# 30230). Specifically ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted using an imidazole gradient and subsequently loaded onto a 5 ml StrepTactin Superflow Cartridge (Qiagen) at a flow rate of 0.8 ml/min ...
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bioRxiv - Molecular Biology 2024Quote: ... The agarose gel bound with the His-tagged recombinant protein was packed in the chromatography column (Qiagen, Germany), and the flow through was discarded ...
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bioRxiv - Immunology 2024Quote: ... tumor protein extracts were generated from snap-frozen biopsies using a combination of TissueLyser LT (Qiagen, Hilden, Germany) and four rounds of freeze/thaw cycling ...
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bioRxiv - Plant Biology 2023Quote: ... All protein sequences (for accession numbers see Supplementary Table 1) were aligned using CLC Genomics Workbench v20.0.4 (Qiagen) and the phylogenetic tree was constructed with the neighbor-joining method using 1000 bootstrap replicates ...
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bioRxiv - Microbiology 2023Quote: ... The N-terminal His-tagged Sif protein (Lmo0946-His6) was expressed and purified using Ni-NTA resin (Qiagen) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... or 0.2 or 0.4 μL of the protein solution and 0.2 μL of a series of commercially available crystallization screens (Qiagen). Conditions that provided initial hits were optimized using bespoke screens prepared at the crystallization facility ...
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bioRxiv - Neuroscience 2023Quote: ... Hippocampi were placed in protein low-binding tubes mounted onto a Qiagen TissueLyser II (Qiagen cat. Nr. 85300). Lysates were span down at 20.000 rcf for 20 minutes at 4°C to remove debris ...
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bioRxiv - Cancer Biology 2023Quote: Enrichment analysis was performed both on the differentially expressed genes and proteins through the use of IPA (QIAGEN Inc. ...
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bioRxiv - Microbiology 2024Quote: ... dialized proteins were supplemented with 10 mM Imidazole and ran over 7.5-mL equilibrated Ni-NTA beads (Qiagen). The flowthrough was collected and concentrated using 30 kDa molecular weight cut-off centrifugal filters (Merck Millipore ...
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bioRxiv - Microbiology 2024Quote: ... dialized proteins were supplemented with 10 mM Imidazole and ran over 7.5-mL equilibrated Ni-NTA beads (Qiagen). The flowthrough was collected and and concentrated using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore ...
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bioRxiv - Cancer Biology 2020Quote: ... APC-negative cells were single-cell sorted by FACS into TCL buffer (QIAGEN) containing 1% 2-mercaptoethanol ...
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bioRxiv - Microbiology 2020Quote: ... virus media was removed and cells were harvested using RNAprotect Cell Reagent (Qiagen). RNA was extracted using RNeasy columns (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: Cells were harvested and resuspended immediately in RNAprotect Cell Reagent (Qiagen, Cat# 76526) for RNA stabilization ...
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bioRxiv - Immunology 2021Quote: The ipsilateral hemispheres were lysed in Qiazol Lysis Reagent and total RNA was extracted using the MaXtract High Density kit with further purification using the RNeasy Mini Kit (all Qiagen). 70 ng of total RNA per sample was then hybridized with reporter and capture probes for nCounter Gene Expression code sets (Mouse Neuroinflammation codeset ...
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bioRxiv - Genetics 2021Quote: ... Cells were lysed with Precellys CKMix Tissue Homogenizing Kit (Bertin Technologies) and total RNA was extracted using Qiagen RNeasy Maxi Kit (Qiagen).
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from lung homogenates according to the instructions of the RNA extraction kit manufacturer (RNeasy Plus Mini Kit; Qiagen) post phase separation using Trizol reagent ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from lung homogenates according to the instructions of the RNA extraction kit manufacturer (RNeasy Plus Mini Kit; Qiagen) post phase separation using Trizol reagent ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA extraction was done using RNeasy Plus Mini Kit following Proteinase K digestion and elimination of DNases using RNase-free DNase kit (all reagents from Qiagen). Isolated RNA was then converted to cDNA using Bio-Rad iScript kit and RT-qPCR using Bio-Rad SYBR Sso Advanced ...
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bioRxiv - Genetics 2019Quote: ... gDNA was extracted from dried blood spots and whole-genome amplified using QIAmp DNA Mini kits and Replica-G Midi amplification kits (Qiagen), respectively ...
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bioRxiv - Cancer Biology 2019Quote: ... DNA and RNA were extracted from sections of the frozen tissue using the QIAamp DNA Mini Kit and RNeasy Mini Kit (Qiagen), respectively ...
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bioRxiv - Genetics 2020Quote: ... Reverse transcription was performed using bead-bound RNA (SuperScript VILO cDNA synthesis kit) and qPCR was performed in triplicate (QuantiFast SYBR Green PCR Kit; Qiagen) on a Roche LightCycler 480 using beta-actin as reference gene ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA extraction and cDNA synthesis were performed the following day using QIAamp RNA Blood Mini Kit and cDNA Synthesis Kit respectively (Qiagen), and then stored at −80°C until assayed ...
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bioRxiv - Microbiology 2020Quote: ... RNA extraction was performed using the QIAamp 96 DNA kit and the Qiacube HT kit and the Qiacube HT (both from Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... RNA was removed using NaOH hydrolysis and cDNA was purified using the buffers from the QIAquick Nucleotide Removal Kit and the columns from the MinElute Reaction Cleanup Kit (Qiagen). Finally ...
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bioRxiv - Genomics 2019Quote: Genomic DNA was extracted from the caudal fin of each challenged fish using the DNeasy Blood & Kit tissue kit (Qiagen), following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... DNA fragments were extracted from agarose gels using the QIAquick gel extraction kit and from liquids using the QIAquick PCR purification kit (QIAGEN). Plasmid DNA was isolated using the Wizard Plus SV Minipreps DNA purification kit (Promega ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... We extracted total genomic DNA from blood and/or tissue samples using standard nucleic acid extraction kits (QIAamp DNA Mini Kit; Qiagen) automated on a QiaCube (Qiagen) ...
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bioRxiv - Neuroscience 2020Quote: Brain samples were lysed in Qiazol Lysis Reagent and total RNA was extracted using the MaXtract High Density kit with further purification using the RNeasy Mini Kit (all Qiagen). 70 ng of total RNA per sample was then hybridized with reporter and capture probes for nCounter Gene Expression code sets (Mouse Neuroinflammation codeset ...
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bioRxiv - Plant Biology 2021Quote: RNA was extracted from ∼100 mg of root tissue and DNase treated using the RNeasy plant mini kit and RNase free DNase kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... sections of small intestine from the lorikeets were deparaffinized and the DNA extracted using a commercial kit (QIAamp DNA FFPE tissue kit; Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... Genomic DNA was extracted 72 hrs post-transfection using a DNA extraction kit (QIAamp DNA Mini kit; Qiagen, Hilden, Germany). This was subjected to a T7E1 assay following the manufacturer’s instructions (New England Biolabs ...
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bioRxiv - Microbiology 2019Quote: Total DNA was extracted from frozen fecal pellets using the PowerSoil-htp 96 well DNA isolation Kit (MoBio) or the DNeasy PowerSoil HTP 96 Kit (Qiagen). Barcoded primes were used to amplify the V3-V4 region of the 16S rRNA gene from extracted bacterial DNA using primers 515fB and 806rB via PCR (EMP) ...