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Citations for Qiagen :
601 - 650 of 6900 citations for Recombinant Human Programmed Cell Death 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: RNA was isolated from 1×103-2.5×106 cells using QIAzol (Qiagen). Total RNA was extracted using miRNeasy Mini kit (Qiagen) ...
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bioRxiv - Genomics 2022Quote: ... total RNA was extracted from 1 million cells using RNeasy columns (QIAGEN). Ribosomal RNA (rRNA ...
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bioRxiv - Genetics 2021Quote: ... for less than 1×104 cells or the RNeasy kit (Qiagen #74004) otherwise ...
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bioRxiv - Genomics 2022Quote: ... Total RNA was extracted from 1 million cells by RNeasy columns (QIAGEN). We used 2 µg of RNA to perform reverse transcription with random primers using TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix TransGen Biotech ...
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bioRxiv - Molecular Biology 2022Quote: ... HL-1 cells were transduced with a pool of 4 gapmers (Qiagen) at 40nM (10Nm each ...
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bioRxiv - Immunology 2022Quote: ... RNA was extracted from 1 × 106 cells using the RNeasy kit (Qiagen), and the heavy and light chain sequences of the murine hybridomas were by obtained using the mouse Ig-primer set (69831 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were resuspended with 1 mL of QIAzol (QIAGEN Inc.; Germantown, MD) and transferred to tissue homogenizing CKMix tubes 2mL (Bertin Instruments ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then stained with 200 µg mL-1 RNaseA (Qiagen, 19101), 50 µg mL-1 propidium iodide (Sigma Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Cells were harvested in 1 mL QIAzol Lysis Reagent (Qiagen, Hilden, Germany) for 5 min at RT and 200 µL chloroform (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... 27620, 1:2,000 dilution) (rabbit anti-Spike S1, Cell Signaling, Cat #: 99423, 1:2,000 dilution) (mouse anti-Strep, Qiagen, Cat #: 34850, 1:2,000 dilution) (rabbit anti-ORF8 ...
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bioRxiv - Genetics 2022Quote: RNA from human ileal biopsies was extracted using RNeasy Mini Kit (Qiagen). 500 ng of RNA was reverse transcribed to cDNA using TaqMan Reverse Transcription kit (#N8080234 ...
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bioRxiv - Cell Biology 2022Quote: FASTQ files generated by sequencing human macrophages were imported into ArrayStudio (Qiagen). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were applied to the Human Aging RT2 Profiler PCR arrays (Qiagen) and run on Bio-Rad CFX384 real time thermocycler ...
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bioRxiv - Molecular Biology 2019Quote: Human islet RNA was extracted using the Qiagen RNeasy Kit (Qiagen; #74106) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Human skin tissues were homogenized with RLT buffer (Qiagen, Hilden, Germany, 79216) supplemented with 1% β-mercaptoethanol (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Primers for human ZMPSTE24 Hs_ZMPSTE24_1_SG QuantiTect) were predesigned and validated by QIAGEN and used diluted to a final work solution of 1x (QuantiTect Primers ...
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bioRxiv - Developmental Biology 2023Quote: FlexiTube siRNA was used to knock-down human PDE2A (Cat#: SI00040159, Qiagen). AllStars Negative CTRL siRNA was used as control (Cat# ...
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bioRxiv - Cell Biology 2024Quote: ... serially diluted matching human PCR amplicons cloned into the pDrive vector (Qiagen) were used to generate a standard curve ...
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bioRxiv - Microbiology 2022Quote: ... 1 mL of the 2-mL culture was removed and replaced by 1 mL of RNAprotect Cell Reagent (Qiagen). After 5 min of incubation ...
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bioRxiv - Genetics 2021Quote: ... The final constructs were transformed into BL21 (DE3) for expression and recombinant Fur/mFur proteins were purified using Ni-NTA agarose (Qiagen, California, USA). The proteins induced by the addition of 1 mM IPTG at 16°C for 12 h ...
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bioRxiv - Microbiology 2021Quote: ... a volume equivalent to 5×108 cells were spun down (assuming 1 OD600=8×108 cells) and Protect Bacteria RNA Mini Kit (Qiagen) was used to extract total RNA as described in (Bhattacharyya et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... shRNA expressing cells were isolated by fluorescence activated cell sorting at passage doubling 1 and 15 followed by genomic DNA isolation with Gentra Puregene cell kit (Qiagen). Sequencing libraries were prepared by subjecting 18 μg (1.2 μg x 15 reactions ...
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bioRxiv - Cell Biology 2022Quote: Genomic DNA was isolated from 1 million cells of each cell line using the QIAamp®DNA Micro Kit (QIAGEN) following the manufacturer’s guidelines ...
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bioRxiv - Immunology 2021Quote: ... DNA was extracted from circulating PD-1+ and PD-1− CD8+ T cells using the All-Prep DNA/RNA Micro Kit from Qiagen and sent to Adaptive Biotechnologies for survey-level TCRβ sequencing.
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bioRxiv - Developmental Biology 2019Quote: Total RNA was extracted from FACS purified Zeb1+ (Lineage−Sca-1+CD49fhi tdTomato+) or Zeb1− basal cells (Lineage−Sca-1+CD49fhitdTomato−) using a RNeasy micro kit (Qiagen). cDNA was synthesized using the PrimeScript RT Reagent Kit (Takara) ...
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bioRxiv - Immunology 2019Quote: ... or M-MØ (1×106 cells/well) were transfected with 1 μg of the XRE reporter construct using either Superfect (Qiagen) or VIROMER RED (Lipocalix) ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with a 1:1 molar ratio of eGFP-P2A-Cas9 and single stranded oligonucleotide using Effectine (Qiagen) in a six-well dish ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated from 1×106 MCF7 and 1×106 BT-474 cells (‘bulk’) using the RNeasy Mini Kit (74104, Qiagen) in combination with the QIAshredder (79654 ...
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bioRxiv - Microbiology 2020Quote: ... Cell lysate was mixed with 1 mL of Ni-NTA agarose beads (Qiagen), and the mixture was rotated at 4 °C for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... The cell pellets were resuspended in 1 ml Qiagen RNA protect reagent (Qiagen) and incubated for 24 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: RPE-1 p53KD cells (parental and clones) were harvested in buffer RLT (Qiagen). Strand-specific libraries were generated using the TruSeq PolyA Stranded mRNA sample preparation kit (Illumina) ...
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bioRxiv - Molecular Biology 2022Quote: RNA was extracted from 1×106 cells with the RNeasy Mini Kit (Qiagen). For qPCR ...
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bioRxiv - Microbiology 2020Quote: ... the cells were washed in 1 mL of RNAprotect solution (Qiagen, Hilden, Germany), pelleted and stored at −80°C until gDNA extraction ...
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bioRxiv - Immunology 2019Quote: Total RNA from 1×106 cells was isolated using RNeasy Mini kit (Qiagen). 50 b.p ...
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bioRxiv - Immunology 2022Quote: Total RNA (from 1 × 106 cells) was isolated with the RNeasy kit (Qiagen) and RNA quality was checked with the Agilent 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA from 1×106 cell was first extracted with RNeasy Plus kit (Qiagen), per manufacturer’s protocol ...
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Reduction of chromosomal instability and inflammation is a common aspect of adaptation to aneuploidybioRxiv - Cell Biology 2023Quote: RPE-1 p53KD cells (parental and clones) were harvested in buffer RLT (Qiagen). Strand-specific libraries were generated using the TruSeq PolyA Stranded mRNA sample preparation kit (Illumina) ...
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bioRxiv - Genetics 2024Quote: ... the cells were lysed in 1 mL of RLT buffer obtained from Qiagen, and for Mass spectrometry ...
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bioRxiv - Molecular Biology 2024Quote: ... castellanii cells at a concentration of 1 μg using Effectene transfection reagent (QIAGEN). Cells were infected with Mimivirus (MOI-10 ...
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bioRxiv - Immunology 2023Quote: Total RNA (from 1 × 106 cells) was isolated with the RNeasy kit (Qiagen) and RNA quality was checked with the Agilent 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Genomics 2023Quote: Approximately 3-4 million cells were solubilized with 1 mL QIAzol (Qiagen, 79306) and 0.2 mL chloroform in 5PRIME Phase-Lock Gel heavy tubes (QuantaBio ...
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bioRxiv - Neuroscience 2021Quote: Cytokine secretion assays were performed using Human Multi-Analyte ELISArray plate (Qiagen CMEH6321A). IL1β ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were prepared using the QIASeq Human Comprehensive Cancer Panel Kit (Qiagen #333515) and sequenced on an Illumina HiSeq 2500 or NovaSeq 6000 ...
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bioRxiv - Pathology 2019Quote: Total RNA of human podocytes was extracted per manufacturer’s instructions (Qiagen, Germantown, MD) and 1 μg RNA was used for cDNA synthesis (Transcriptor first strand cDNA synthesis kit ...
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bioRxiv - Genomics 2019Quote: We analyzed human and mouse Tug1 cDNA sequences with CLC Genomics Workbench (Qiagen) for open reading frames (ORFs) ...
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bioRxiv - Neuroscience 2022Quote: ... using commercially available primers for human FAAH (CpG Island 100530) (EPHS100530-1A, Qiagen). Methylation-sensitive (EPHS115450-1A ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was extracted from human thyroid tissues using miRNeasy Mini kit (Qiagen) according to the manufacturer’s instructions and as described [26] ...
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bioRxiv - Neuroscience 2023Quote: ... Human rRNA depletion was performed using the QIASeq FastSelect kit (Qiagen, Hilden, Germany). RNA sequencing library preparation used NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s recommendations (New England Biolabs ...
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bioRxiv - Immunology 2023Quote: DNA from human stool samples was extracted with DNeasy PowerSoil Pro kit (Qiagen) according to the manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2022Quote: Total RNA for human TSCs was extracted using RNeasy Micro kit (Qiagen, 74004) with on-column DNase digestion ...