Labshake search
Citations for Qiagen :
601 - 650 of 3615 citations for 6 Bromo 3 3H imidazol 4 ylmethylene 1 3 dihydro indol 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2023Quote: DNA extraction was carried out using one of the following methods: 1) QIAmp DNA Mini Kit (Qiagen, Germany); 2 ...
-
bioRxiv - Cell Biology 2020Quote: Genomic DNA was extracted from 2×105 primary human lung fibroblasts isolated from fresh and cryopreserved tissue from 3 different donors in technical replicates using QIAamp Micro Kit (Qiagen, Hilden, Germany) following manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... and each amoeba-adapted and non-adapted population derived from days 3 and 42 using the QIAamp DNA mini kit (Qiagen, Venlo, Netherlands) according to manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2021Quote: ... RNA was extracted from each sample (n=3 WT and n = 3 Fgf9null) using Trizol/chloroform and spin-column assembly with on-column DNase treatment (RNeasy mini kits; Qiagen Germantown, MD). We assessed the quality of RNA using fragment analysis to obtain RNA integrity number (RIN ...
-
bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from control and treated samples at 3 and 24 hr with three biological replicates (Figure S1) using Qiagen RNeasy Plant Mini kit (Qiagen, Hilden, Germany), with an on-column DNase treatment ...
-
bioRxiv - Genomics 2020Quote: ... Sachinoka plantlets grown in vermiculite under 12 h light/12 h dark conditions at 22°C were isolated using the improved 3% CTAB3 method (Yu et al. 2012) followed by treatment with RNase-free DNase (QIAGEN, Venlo, Netherlands) and clean up with RNeasy spin columns (QIAGEN ...
-
bioRxiv - Genomics 2020Quote: ... 3 μl of the PCR products was mixed and purified using the QIAquick PCR Purification Kit (Catalog No. 28104, Qiagen, Hilden, Germany).
-
bioRxiv - Cancer Biology 2020Quote: ... the pCMV-Neo-Bam p53 R248W vector containing the ampr gene was purified from the transfected SKOV-3 cells using the Qiaprep Miniprep Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2019Quote: Total RNA from magnetically purified NDC, PSEN1M146L, and PSEN1A246E (replicates, n = 3) human iPSC-derived neurons was prepared using miRNeasy Micro Kit (Qiagen Cat. 217084), based on manufacturer’s procedures ...
-
bioRxiv - Neuroscience 2019Quote: Total RNA from magnetically purified NDC, PSEN1M146L, PSEN1A246E, and PSEN1H163R (replicates, n = 3) human iPSC-derived neurons was prepared using RNeasy Plus Micro Kit (Qiagen, Cat. 74034) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: DNA was extracted from 3 ml of each raw milk sample using the PowerFood™ Microbial DNA Isolation Kit (MoBio Laboratories, Qiagen, USA) following the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2020Quote: Tocochromanols in 50.3 ± 0.4 mg DW of ground seed powder were extracted in 750 μL of icecold heptane, using two 3-mm diameter glass beads (Carl Roth GmbH+Co, Karlsruhe, Germany) and a Tissue-Lyser (Qiagen, Hilden, Germany) at 25 Hz for 2 min ...
-
bioRxiv - Plant Biology 2021Quote: ... 3 leaf discs per sample from different plants were collected in microfuge tubes and ground with a tissue lyser (Qiagen, Düsseldorf, Germany). Serial dilutions were plated on LB agar before counting colonies ...
-
bioRxiv - Neuroscience 2022Quote: mRNA samples from ANS4-GFP and ANS4-GFP cells co-cultured with bEnd.3 (for 72 hours) were isolated using RNeasy Plus Micro Kit (QIAGEN, cat# 74034). Next-generation whole transcriptome Illumina sequencing (HiSeq4000 ...
-
bioRxiv - Microbiology 2021Quote: ... Liquid cultures or swabs from agar plates from selected bacterial strains (Supplementary Data 3) were used to extract DNA using the QiAmp Micro DNA kit (Qiagen, Hilden, Germany). The extracted DNA was treated with RNase ...
-
bioRxiv - Microbiology 2019Quote: ... and then amplified using 40 cycles of 95°C for 3 s and 60°C for 30 s using either the Rotor-Gene Q or Rotor-Gene 6000 platform (Qiagen, Hilden, Germany).
-
bioRxiv - Microbiology 2019Quote: ... The tube was subjected to bead beating for 3 x 45 seconds at 30 m/s in a TissueLyser II (Qiagen, Hilden, Germany) and placed on ice for 2 mins before repeating ...
-
bioRxiv - Microbiology 2021Quote: ... Blood was obtained for lymphocyte preparation 3-5 days after the fifth immunization and RNA was prepared from lymphocytes using the RNeasy kit (Qiagen, Valencia, CA). A VHH-display phage library was prepared essentially as described previously [70] following each of the rounds of alpaca immunization ...
-
bioRxiv - Synthetic Biology 2022Quote: ... A 150 bp galactose operon-specific fragment containing the region where the predicted RNA 3′-end is located was amplified using HotStarTaq™ DNA polymerase (QIAGEN, Germany) with gal-specific primer M3-F and 3RP primer (Table S3) ...
-
bioRxiv - Microbiology 2022Quote: ... 2 g of soil was immediately transferred into pre-weighed sterile tubes containing 3 ml of LifeGuard soil preservation solution (Qiagen, Toronto, CA) to stabilize the RNA ...
-
bioRxiv - Microbiology 2023Quote: ... 293A cells were transfected with the plasmid of pCDNA3.1-ACE2 (human)-3×FLAG (MiaoLing Plasmid Platform, China) using polyfect (QIAGEN, West Sussex, UK) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: 4.5×106 - 7.3×106 live control or oxPAPC-treated Tregs were harvested on day 3 of culture and RNA was isolated using RNeasy Plus Mini Kit (Qiagen Cat #: 74134) according to manufacturer instruction ...
-
bioRxiv - Evolutionary Biology 2022Quote: RNA was isolated from abdomens from 3 days post injection pupae collected during the experimental infection experiments using the RNeasy Mini Kit (Qiagen, Hilden, Germany) following manufacturers’ protocol including a DNAse incubation step and quantified using a Nanodrop ...
-
bioRxiv - Cancer Biology 2023Quote: ... BRD4-S (targeting short variant 3’-UTR region; Cat#: SI05044865) and AllStars Negative control (Cat. #: SI03650318) were purchased from QIAGEN (MD, USA). ITGA4 siRNA (Cat ...
-
bioRxiv - Genetics 2023Quote: ... 1 mL glycerol stocks were made for each library and a 200 mL LB-AMP outgrowth was grown to a concentration of 3 OD600/mL followed by a Qiagen MAXI plasmid prep (Qiagen Cat#12963).
-
bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from 3 mL overnight cultures of each strain (see Strains Cultivation) using the DNeasy PowerSoil Pro Kit from Qiagen (Hilden, Germany) per manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2022Quote: Total RNA from 18 ONS cell samples (6 AD patients, 6 MCI and 6 HC) were extracted using Allprep universal kit (Qiagen cat. no. 80224). RNA-seq libraries were prepared using the Illumina TruSeq Stranded Total RNA library Prep Gold Kit (Illumina 20020598 ...
-
bioRxiv - Systems Biology 2020Quote: ... 6×10^6 PBMC from each sample were transferred directly to Quiazol regent (QIAGEN), resuspended and immediately aliquoted and stored at −80°C until RNAseq downstream processing ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... One million cells per well were seeded in 6 well plates in 2 mL growth medium the day before transfection and transfected with PolyFect (Qiagen, Düsseldorf, DE) using the manufacturers protocol (changing the medium to growth medium without hygromycin B and puromycin prior to transfection) ...
-
bioRxiv - Molecular Biology 2020Quote: 6 ml Ni-NTA (QIAGEN) was washed with 5 column volumes of wash buffer 1 (150 mM NaCl ...
-
bioRxiv - Microbiology 2020Quote: ... using One Step RT-PCR kit (Qiagen, Germany) and its larger portion was amplified for phylogenetic analysis by self designed primers ...
-
bioRxiv - Biochemistry 2021Quote: ... one 5mm stainless steel tissue homogenizer bead (Qiagen) was added to each tube ...
-
bioRxiv - Microbiology 2021Quote: ... with one-hour in-tube DNase digestion (Qiagen) to remove possible DNA contamination according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... in one-step probe RT-qPCR kits (Qiagen) with the following cycle conditions ...
-
bioRxiv - Microbiology 2020Quote: ... in one-step probe RT-qPCR kits (Qiagen) with the following cycle conditions ...
-
bioRxiv - Microbiology 2021Quote: ... in one-step probe RT-qPCR kits (Qiagen) with the following cycle conditions ...
-
bioRxiv - Biochemistry 2019Quote: ... Bound STAT3 proteins were then labeled with Alexa488-conjugated anti-6 × His antibodies (1 hr incubation, 1:20 dilution, Qiagen 35310). The array was washed and scanned using a GenePix 4400A scanner (Molecular Devices ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2 μl of diluted cDNA template was mixed with 4 μl QuantiTect SYBR Green PCR Master Mix (Qiagen), 100 nM forward primers (see Table 1 for sequences ...
-
bioRxiv - Immunology 2023Quote: ... 14-666-315) containing 1 mL sterile PBS and one 5 mm stainless steel bead (QIAGEN Cat. No. 69989). Nucleic acids were isolated from excised granulomas by homogenizing in TRIzol (Invitrogen ...
-
bioRxiv - Genomics 2021Quote: ... PI-negative fraction was sorted in each well of a 96 well plate containing 3 μL of PBS (Qiagen, REPLI-g SC Kit) with 0.2% Tween 20 ...
-
bioRxiv - Molecular Biology 2019Quote: ... The miRNAs were modified by polyadenylation at the 3’ end and then reverse transcribed into the cDNA of miRNA using the miScript PCR Starter Kit (#218193, Qiagen, Valencia, CA, USA) with an oligo dT primer (with a universal tag) ...
-
bioRxiv - Plant Biology 2021Quote: ... and sunflower_KTI _R (5’ – CTACTCAGATTGAACAGAAGCCAC- 3’were designed and used in a PCR reaction with total DNA extracted (DNeasy Plant Mini Kit, Qiagen, Valencia CA USA) from sunflower leaf tissue ...
-
bioRxiv - Plant Biology 2021Quote: RNA samples were isolated from seedlings at each time point with 3 biological replicates using Qiagen Plant RNA extraction kit (Qiagen; catalog no. 74904). A total of 18 cDNA libraries were prepared following the standard BGISEQ-500 RNA sample preparation protocol and sequenced by the DNBseq platform (BGI ...
-
bioRxiv - Microbiology 2022Quote: Total mouse lung RNA was extracted at days 0 and 3 post-challenge using the RNeasy Mini Kit as described above (QIAGEN, Cat. No 74004) and purified with the QIAquick PCR Purification Kit (QIAGEN ...
-
bioRxiv - Systems Biology 2022Quote: Total RNA was isolated from whole brain tissue from 3 biological replicates per strain for 45 strains of the HRDP using QIAzol (Qiagen, Valencia, CA, USA). The brain was split sagitally and half of the brain was used for RNA sequencing ...
-
bioRxiv - Cancer Biology 2023Quote: ... The resultant backbone without the Puromycin gene was purified using the QIAquick PCR & Gel Cleanup Kit (Qiagen 28506. A DNA block (Table 3) containing the Blasticidin resistance gene sequence was synthetised (Integrated DNA Technologies ...
-
bioRxiv - Immunology 2022Quote: ... After diluting samples in a 4:1 ratio (elution buffer [Qiagen]:cDNA), cDNA concentration was determined using a Bioanalyzer (Agilent Technologies).
-
bioRxiv - Molecular Biology 2022Quote: ... HL-1 cells were transduced with a pool of 4 gapmers (Qiagen) at 40nM (10Nm each ...
-
bioRxiv - Cell Biology 2022Quote: ... with 1% 2-mercaptoethanol then passed through Qiashredder tubes (Qiagen). RNA was extracted using the RNeasy Isolation Kit (Qiagen ...
-
bioRxiv - Microbiology 2022Quote: ... loaded with 1-2 ml Ni-NTA agarose resin (Qiagen) pre-equilibrated with binding buffer ...