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Citations for Qiagen :
6351 - 6400 of 10000+ citations for TBARS MDA Universal Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... 2 minutes) and the supernatant treated with Solution C2 and C3 according to the DNeasy PowerSoil protocol (Qiagen). The resulting supernatant was combined with the original filter extract supernatant before DNA purification ...
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bioRxiv - Immunology 2021Quote: ... Both barcode and digested fragment products were run on a 2% gel and purified by gel extraction (Qiagen). NGS library was sequenced using an Illumina MiSeq and Illumina v3 MiSeq Reagent Kits with 150 base pair single-end sequencing according to standard manufacturer protocols ...
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bioRxiv - Physiology 2020Quote: Purified total RNA was extracted from fresh BM-MSCs at passage 2 using a Qiagen Minikit (Qiagen, Australia). Quantitative RT-PCR was performed (n=3 mice/group ...
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bioRxiv - Physiology 2020Quote: ... Groups of five flies were transferred to 2 mL tubes and were homogenized in 100μl PBS + 0.1% Triton X-100 using a TissueLyser LT (Qiagen). The samples were then spun down at 12,000 rpm and the supernatant transferred to fresh vials and the absorbance of each sample at 603 nm was measured using a NanoDrop ...
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bioRxiv - Microbiology 2021Quote: Frozen plant tissue was ground using the TissueLyser II (QIAGEN; 2 cycles of 30 seconds at 27 Hz) and 3-mm zirconium oxide beads (Glen Mills Inc.) ...
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bioRxiv - Microbiology 2022Quote: ... using a MiSeq reagent kit v3 with 2 × 75 paired end-reads and sequence analysis was performed using CLC-Genomics workbench (v10.1.1., Qiagen). Enrichment in reads of each sample was compared to the corresponding control and mapped as peaks against the annotated genome of S ...
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bioRxiv - Physiology 2022Quote: ... approximately 100 mg of adipose tissue and a 7 mm metal bead were placed in a 2 mL centrifuge tube containing 0.5 mL PBS buffer (pH 7.4) and homogenised in TissueLyser II (Qiagen) for 2 minutes at 25 Hz ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was placed into 2-mL round-bottom homogenizer tubes pre-loaded with Stainless Steel beads (#69989; Qiagen) and filled up with lysis buffer (#AA-LYS-16ml ...
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bioRxiv - Immunology 2022Quote: ... diluted in Milli-Q H2O 1:2 and the cells disrupted by mechanical lysis in the TissueLyser (Qiagen) for 2 minutes at 30 Hz and three freeze/thaw cycles (20° to -20°C).
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bioRxiv - Microbiology 2022Quote: ... Primary-pools of ants were homogenized with 2 ceramic beads (2.8mm) in 350 µl QIAzol Lysis Reagent (Qiagen) for 2×2min at 30Hz in a TissueLyser II (Qiagen) ...
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bioRxiv - Molecular Biology 2024Quote: ... ∼80 uL packed irradiated embryos and control embryos were resuspended in 2 mL buffer G2 (Qiagen, catalog # 1014636) and digested with proteinase K (1 mg/mL ...
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bioRxiv - Immunology 2024Quote: ... 2 × 104 cells were sorted in triplicate per sample and stored at −80°C in RLT buffer (Qiagen). RNA was isolated using the Qiagen RNeasy Micro Kit ...
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bioRxiv - Microbiology 2023Quote: ... Mosquito samples were homogenized at 30 Hz for 2 minutes using a TissueLyser II (QIAGEN GmbH, Hilden, Germany) and centrifuged for 60 s at 11,000 rpm ...
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bioRxiv - Microbiology 2023Quote: ... 150 μL of the aggregated or non-aggregated cells were then washed with 2 volumes of RNAprotect (Qiagen) to prevent RNA degradation ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μl of diluted cDNA template was mixed with 4 μl QuantiTect SYBR Green PCR Master Mix (Qiagen), 100 nM forward primers (see Table 1 for sequences ...
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bioRxiv - Microbiology 2023Quote: ... and homogenised with 3-5 mm steel beads in a TissueLyser II (Qiagen, 24 Hz, 2 × 3 min). 350 μL lysed blood or 200 μL lysed head kidney were transferred to a Magna Pure 96 Processing Cartridge (Roche) ...
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bioRxiv - Genomics 2023Quote: ... The mixture was then transferred to a 2 mL MaXtract High Density tube (Cat#129056, Qiagen, Hilden, Germany) for phase separation ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% CA630) and rotated at 4°C for 2 hours before addition of His-beads (Ni-NTA, QIAGEN). After continuous rotating for another 2 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... with the addition of a 2-minute spin at 15,000 x g in the QIAshredder (Qiagen, Germantown, MD) prior to the genomic DNA elimination step ...
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bioRxiv - Genomics 2024Quote: ... Amplicons were generated in 2 different 25 µL PCR reactions (9.5 µL nuclease-free water, 12.5 µL 2x QIAGEN Multiplex PCR Master Mix ...
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bioRxiv - Developmental Biology 2024Quote: ... 200 ng psiCHECK-2 reporter plasmid were co-transfected with 5 µl miScript miRNA mimics (Qiagen; CatNo. 219600) using 4 µl Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from leaves and roots samples by using The Plant RNeasy extraction kit (RNeasy Plant Mini Kit, Qiagen, Valencia, CA, USA). To remove any residual genomic DNA ...
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bioRxiv - Genomics 2019Quote: DNA was extracted from individual mosquitoes for all Eurasian samples using the DNA Invisorb Spin Tissue Mini Kit (Invitek, Berlin, Germany) and for all American samples using the Qiagen Blood and Tissue Kit (Qiagen, Germantown, MD, USA). DNA concentration was determined by nanodrop ...
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bioRxiv - Cancer Biology 2021Quote: Total DNA from xenograft tissue samples was isolated using a commercially available column-based purification kit from QIAGEN (DNeasy Blood and Tissue Kit) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Further processing was performed with agreement of the RNA extraction protocol using the Qiagen Purification of Total RNA Kit for Yeast with Optimal On-Column DNase Digestion (RNeasy Mini Kit (50) (Qiagen, catalog no. 74104).
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bioRxiv - Molecular Biology 2022Quote: ... and the DNA pellet was stored at −80°C until purification was performed using a commercial kit (DNeasy Blood and Tissue Kit, catalog #69504; Qiagen; Germantown, MD, USA) according to manufacturer’s instructions with a minor modification to the elution step ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA was extracted from each sample using a miRNeasy Mini Kit and a RNeasy MinElute Cleanup Kit (both from (Qiagen, Valencia, CA, USA)) ...
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bioRxiv - Cell Biology 2020Quote: ... genomic DNA were labeled by nick translation to generate green fluorescent probes using Atto488 Nick Translation Labeling Kit (Jenabioscience) and purified by silica-gel membrane adsorption (Qiagen PCR Purification Kit), according to the kit protocols ...
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bioRxiv - Epidemiology 2020Quote: Total RNA was extracted from tissue and serum samples using two commercial kits according to the manufacturer’s instructions: QIAamp® RNA Blood for tissues and QIAamp® Viral RNA Kit for serum (Qiagen Inc., Germany). Viral RNA was detected using two previously published RT-qPCR techniques (16,17).
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bioRxiv - Immunology 2019Quote: Viral RNA from serum and cellular RNA from tissues collected at various time-points from both ZIKV-infected and uninfected animals were extracted using QIAamp Viral RNA mini kit and RNeasy mini kit respectively (Qiagen, Valencia, CA, USA). The extracted RNA was amplified by iTaq universal SYBR Green one-step PCR kit (Bio-Rad ...
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bioRxiv - Genomics 2021Quote: ... DNA was removed with the Ambion® DNA-free™ Kit following the standard procedure and purified with the RNeasy® Mini Kit (QIAGEN).
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bioRxiv - Biochemistry 2021Quote: RNA from both rabbits was extracted from lymph nodes and spleen using the RNeasy mini kit and from blood samples using the RNeasy Protect Animal Blood kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer′s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and plasma membrane proteins were purified from cultured astrocytes using a commercially available kit (Qproteome Cell Compartment Kit; Qiagen, Inc, Germantown, MD, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... while the marine and freshwater samples were extracted using the DNeasy® PowerWater® Kit and RNeasy® PowerWater® Kit (QIAGEN, Germany). The extracted nucleic acid was then subject to library construction using NEBNext Ultra RNA Library Prep Kit and NEB Next Ultra DNA Library Prep Kit (LTD.NEB ...
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bioRxiv - Evolutionary Biology 2024Quote: ... flies were separated in to four pools of 25 and DNA was extracted using a column-based DNA extraction kit (DNeasy Qiagen kit, Cat # 69506). The extracted DNA from each of the four pools of 25 for distinct samples were combined such that the same concentration of DNA was added from each pool (equimolar) ...
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bioRxiv - Immunology 2023Quote: ... The crypts were placed in lysis buffer (RLT, provided in RNeasy® Mini Quiagen Kit, 74104) and RNA was isolated following manifacturer instructions (RNeasy® Mini Kit, Qiagen, 74104). RNA quantification was done by spectrophotometry (ND1000 Spectrophotometer ...
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bioRxiv - Cancer Biology 2023Quote: ... we isolated viral RNA from xenograft tumors using either a Qiagen viral RNA kit or Trizol followed by the Qiagen viral RNA kit (Qiagen, Germantown, MD, USA). The isolated RNA was then submitted to the University of Minnesota Genomics Center (UMGC ...
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bioRxiv - Microbiology 2024Quote: Bacterial pellets were thawed at room temperature and DNA was extracted using a commercially available extraction kit (DNeasy® PowerSoil ® Pro Kit, QIAGEN, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... were PCR amplified (see Table S4 for primer sequences) from 3D7 or IT4 gDNA (Monarch gDNA Purification Kit, NEB or QIAGEN DNA extraction kit) and cloned into pSLI (Birnbaum et al. ...
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bioRxiv - Cell Biology 2022Quote: ... After 72 h cells were passaged onto new 6-well plates and culturing continued for an additional 72 h after which cells were harvested in Buffer RLT (Qiagen; for RNA-sequencing, RT-qPCR) or used for cell culture experiments.
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bioRxiv - Microbiology 2019Quote: Community DNA from Lake Matano sediment enrichments was extracted from 2◻g samples and purified using a PowerSoil Isolation Kit and UltraClean® 15 Purification Kit (formerly MO BIO Laboratories, now Qiagen, Carlsbad, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 1-4 legs or tissue teased from a single chelicera from single specimens were used for DNA extractions using the DNeasy™ Blood and Tissue kit and the QIAamp DNA Mini kit (Qiagen Inc., Valencia, CA). Libraries were prepared and enriched following protocols outlined by (Kulkarni et al ...
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bioRxiv - Microbiology 2019Quote: ... DNA or total RNA was extracted from fresh tissue or FFPE samples using QIAamp® DNA Mini Kits or RNeasy® Mini Kits from Qiagen (Hilden, Germany), according to the manufacturer’s instructions – with modifications as described previously (9) ...
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bioRxiv - Biophysics 2021Quote: ... The solubilized fraction was then incubated for 2 h with 300 µL of pre-equilibrated Ni-NTA resin (QIAGEN) in Ni-NTA buffer (50 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2021Quote: ... the second PCR used 2 µL of the first reaction as template and HotStarTaq DNA Polymerase (Qiagen, Hilden, Germany) in a 20 µL reaction volume for 15 cycles ...
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bioRxiv - Neuroscience 2022Quote: RNA was isolated from snap frozen striatal mouse tissue from 2 independent cohorts of mice according to the manufacturer’s protocol and purified using RNeasy columns (Qiagen). A total of 13 mRNA libraries for Illumina sequencing were prepared using KAPA mRNA HyperPrep Kit (KAPA Biosystems ...
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bioRxiv - Genomics 2019Quote: ... is manually added to each well using a multichannel pipette followed by 2 µL of lysis buffer (0.2 µL of 25 µg/µL Qiagen Protease ...
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bioRxiv - Systems Biology 2019Quote: ... ∼20 mg frozen tissue was pulverised in chloroform-methanol (400 µl; 2:1 v/v) using a TissueLyser (Qiagen), then the mixture was sonicated for 10 minutes ...
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bioRxiv - Genomics 2019Quote: Cohort 2: Whole blood was collected from a separate cohort of 18 subjects within PAXgene Blood ccfDNA Tubes (Qiagen). Blood plasma was separated by centrifugation for 15 minutes at 1900g and clarified with a second centrifugation for 10 minutes at 1900 x g ...
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bioRxiv - Neuroscience 2020Quote: ... each individual cell was transferred to a 0.2 ml PCR tube containing 2 μl of lysis buffer (RLT Plus - Qiagen). The tube was immediately placed on a metal plate sitting on top of dry ice for flash-freezing ...