Labshake search
Citations for Qiagen :
6251 - 6300 of 10000+ citations for C Reactive Protein ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... while the marine and freshwater samples were extracted using the DNeasy® PowerWater® Kit and RNeasy® PowerWater® Kit (QIAGEN, Germany). The extracted nucleic acid was then subject to library construction using NEBNext Ultra RNA Library Prep Kit and NEB Next Ultra DNA Library Prep Kit (LTD.NEB ...
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bioRxiv - Evolutionary Biology 2024Quote: ... flies were separated in to four pools of 25 and DNA was extracted using a column-based DNA extraction kit (DNeasy Qiagen kit, Cat # 69506). The extracted DNA from each of the four pools of 25 for distinct samples were combined such that the same concentration of DNA was added from each pool (equimolar) ...
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bioRxiv - Immunology 2023Quote: ... The crypts were placed in lysis buffer (RLT, provided in RNeasy® Mini Quiagen Kit, 74104) and RNA was isolated following manifacturer instructions (RNeasy® Mini Kit, Qiagen, 74104). RNA quantification was done by spectrophotometry (ND1000 Spectrophotometer ...
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bioRxiv - Cancer Biology 2023Quote: ... we isolated viral RNA from xenograft tumors using either a Qiagen viral RNA kit or Trizol followed by the Qiagen viral RNA kit (Qiagen, Germantown, MD, USA). The isolated RNA was then submitted to the University of Minnesota Genomics Center (UMGC ...
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bioRxiv - Microbiology 2024Quote: Bacterial pellets were thawed at room temperature and DNA was extracted using a commercially available extraction kit (DNeasy® PowerSoil ® Pro Kit, QIAGEN, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... were PCR amplified (see Table S4 for primer sequences) from 3D7 or IT4 gDNA (Monarch gDNA Purification Kit, NEB or QIAGEN DNA extraction kit) and cloned into pSLI (Birnbaum et al. ...
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bioRxiv - Systems Biology 2020Quote: ... was prepared by centrifuging a cell culture at 5525xg for 2 minutes at 4°C then resuspending cells in 1mL of PBS-RNAprotect (333 μL RNAprotect Bacteria Reagent [76506, Qiagen, Hilden, Germany], 666 μL PBS). For immediate RNA stabilization by RNAprotect (Fig ...
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bioRxiv - Cell Biology 2020Quote: ... The relative expression of mRNAs of c-Myc regulated genes identified in RT2 Profiler™ PCR Array Human MYC Targets (Qiagen, Cat. no. PAHS-177Z) was determined by real-time quantitative RT-PCR ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed twice with the blocking buffer and incubated at 4 °C with primary antibodies (mouse anti-Strep, Qiagen, Cat #: 34850, 1: 150 dilution) (rabbit anti-PDI ...
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bioRxiv - Genetics 2019Quote: ... protein altering inframe InDels (n=28) and missense variants (n=557) were uploaded to Ingenuity pathway Analysis (Qiagen, Venlo, The Netherlands). Additionally ...
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bioRxiv - Biochemistry 2020Quote: ... Canonical pathways associated with differentially expressed proteins were identified by Ingenuity Pathway Analysis (IPA, Ingenuity Systems, Qiagen, Redwood, CA, USA; www.ingenuity.com). This program is based on an algorithm that uses curated connectivity information from the literature to determine the network of interactions among the differentially expressed proteins and canonical pathways in which they are involved (Calvano et al. ...
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bioRxiv - Plant Biology 2021Quote: ... the cultured bacteria cells were lysed by using a high-pressure cell crusher and the fusion proteins were purified with Ni-NTA resin (Qiagen, www.qiagen.com) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... Recombinant protein in the supernatant was purified to 90% purity by a single-step chromatography on Ni++ - metal chelate affinity (Qiagen, UK) columns and eluting at 0.5 M imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Matrix crystallization screens were performed at room temperature using the sitting drop vapor diffusion method by mixing equal volumes of protein sample and reservoir using a TTP LabTech Mosquito robot and commercially-available screens (Hampton Research and Qiagen). Initial hits were optimized and crystals were obtained in 20% PEG 3350 at 20 °C ...
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bioRxiv - Immunology 2022Quote: Initial matrix crystallization trials against 576 conditions were performed at room temperature using the sitting drop vapor diffusion method by mixing equal volumes of protein sample and reservoir using a TTP LabTech Mosquito robot and commercially-available screens (Hampton Research and Qiagen). Initial hits were optimized and crystals for unliganded BG24S60A were obtained in 0.25 M potassium chloride ...
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bioRxiv - Biophysics 2020Quote: ... Bacterial extracts were prepared as described46 and the recombinant protein was purified using an Ni-NTA Superflow cartridge (Qiagen, Hilden, Germany) and eluted with imidazol47 ...
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bioRxiv - Biochemistry 2019Quote: ... Bound STAT3 proteins were then labeled with Alexa488-conjugated anti-6 × His antibodies (1 hr incubation, 1:20 dilution, Qiagen 35310). The array was washed and scanned using a GenePix 4400A scanner (Molecular Devices ...
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bioRxiv - Biochemistry 2019Quote: ... The soluble 6His-tagged proteins were purified from the supernatant by affinity chromatography using Ni2+-NTA agarose resin (Qiagen, Hilden, Germany). After several washing steps ...
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bioRxiv - Cell Biology 2021Quote: ... Single cell clones were grown for immunoblot analysis and those showing loss of ATF4 protein were selected for sequence analysis involving the isolation of genomic DNA (Qiagen, 69504), PCR amplification using KOD Xtreme™ Hot Start DNA Polymerase (Millipore ...
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bioRxiv - Immunology 2021Quote: ... The plasmid was transfected to 293T cells and the recombinant S protein trimers were purified by Ni-NTA (nickel-nitrilotriacetic acid) chromatography (QIAGEN, Germany), followed by size exclusion to further purify the trimers ...
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bioRxiv - Synthetic Biology 2023Quote: ... followed by incubations with a PBS-based protein binding mixture for 1 h and with Alexa488-conjugated anti-His antibody (1:20 dilution, Qiagen 35310) for 1 h ...
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bioRxiv - Synthetic Biology 2023Quote: ... bacteria were pelleted at 3000 rcf and purified following the QiaExpress Ni-NTA Fast Start protein purification protocol (Qiagen, Catalog # 30600) under native conditions.
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bioRxiv - Immunology 2023Quote: ... Functional pathway and network analysis of differentially expressed proteins were performed using Ingenuity Pathway Analysis (IPA) (Qiagen, Redwood City, CA, USA). The pathway and network score were based on the hypergeometric distribution and calculated with the right-tailed Fisher’s exact test.
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bioRxiv - Cell Biology 2023Quote: ... Cat# 28-9537- 66) was used and Streptavidin tagged proteins were purified using Strep-Tactin® Superflow® Plus cartridge (QIAGEN). Columns were attached to MINIPULS® 3 peristaltic pump (Gilson ...
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bioRxiv - Molecular Biology 2023Quote: ... 40% of the protein material of each fraction was analyzed by Western blot using an anti-his antibody (Penta-His antibody, Qiagen #34660). Detection was performed with a secondary antibody conjugated with horseradish peroxidase (Anti-Mouse IgG Peroxidase antibody ...
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Macrodomain Mac1 of SARS-CoV-2 Nonstructural Protein 3 Hydrolyzes Diverse ADP-ribosylated SubstratesbioRxiv - Biochemistry 2023Quote: ... Frozen cells were thawed on ice and used for protein purification with nickel-nitrilotriacetic acid (Ni-NTA) Fast Start column as recommended by the manufacturer (Qiagen, Germany). The bound proteins were eluted from the column with elution buffer containing 50 mM NaH2PO4 ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was purified from soluble fraction under native conditions using Ni2+– NTA affinity chromatography (Ni2+ beads obtained from Qiagen, #30210), followed by a second step of purification involving gel filtration chromatography using Superdex 200 10/300 GL (GE Healthcare ...
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bioRxiv - Plant Biology 2024Quote: ... The induction and purification of the recombinant protein was carried out under native conditions according to the QIAexpressionist manual (“Qiagen”, Germany). For the purpose of fusion protein purification from bacterial cells 1000 ml LB growth medium was inoculated by 25 ml of overnight E ...
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bioRxiv - Microbiology 2019Quote: Community DNA from Lake Matano sediment enrichments was extracted from 2◻g samples and purified using a PowerSoil Isolation Kit and UltraClean® 15 Purification Kit (formerly MO BIO Laboratories, now Qiagen, Carlsbad, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 1-4 legs or tissue teased from a single chelicera from single specimens were used for DNA extractions using the DNeasy™ Blood and Tissue kit and the QIAamp DNA Mini kit (Qiagen Inc., Valencia, CA). Libraries were prepared and enriched following protocols outlined by (Kulkarni et al ...
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bioRxiv - Microbiology 2019Quote: ... DNA or total RNA was extracted from fresh tissue or FFPE samples using QIAamp® DNA Mini Kits or RNeasy® Mini Kits from Qiagen (Hilden, Germany), according to the manufacturer’s instructions – with modifications as described previously (9) ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was extracted using the RNeasy Mini Kit (Qiagen) as reported previously (39 ...
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bioRxiv - Plant Biology 2020Quote: ... cleaned up using RNeasy MinElute Cleanup Kit (QIAGEN, Catalog # 74204), and tested for RNA integrity using an Agilent Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were purified using QIAprep Spin Miniprep kit (Qiagen) for Sanger sequencing (Macrogen) ...
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bioRxiv - Plant Biology 2020Quote: ... and DNeasy® Plant Mini Kit (Qiagen, Valencia, CA, USA), respectively ...
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bioRxiv - Microbiology 2020Quote: ... Tagmented DNA was then purified a Minelute purification kit (Qiagen). DNA fragments were PCR amplified using dual barcoded primers and repurified using a Minelute kit followed by a 1.5x final cleanup step with Ampure SPRI beads (Agencourt) ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was isolated using RNeasy Plant Mini Kit (Qiagen). RNA (4 μg ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified with MinElute PCR purification kit (Qiagen) according to manufacturer’s protocol and eluted in 16 µl of elution buffer ...
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bioRxiv - Microbiology 2020Quote: ... The product was purified by the PCR purification kit (Qiagen) and cut by XbaI and HindIII (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... followed by processing with an RNeasy Mini Plus kit (Qiagen). For analyzing gene expression by quantitative real-time PCR ...
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bioRxiv - Microbiology 2020Quote: ... with a MagAttract PowerSoil KF Kit (Qiagen NV, Venlo, Netherlands) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... and then reverse transcribed using QuantiTect Reverse Transcription Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... RNA was isolated with the RNeasy™ Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA was isolated with RNeasy Plus Mini Kit (Qiagen) and libraries were prepared with Zymo-Seq RiboFree Total RNA Library Kit (Zymo) ...
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bioRxiv - Cancer Biology 2021Quote: ... PCR products were purified with QIAquick PCR purification kit (Qiagen), ligated to Topo Blunt vector (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA extraction was run using RNeasy Plus kit (QIAGEN) which includes an optional DNAse digestion ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA was isolated using RNeasy RNA isolation kit (Qiagen) according to the instructions of the manufacturer ...
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bioRxiv - Cancer Biology 2021Quote: ... and the RNeasy Plus RNA Extraction Kit (Cat# 74134, Qiagen). Two biological replicates were collected for each sample ...
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bioRxiv - Cancer Biology 2021Quote: ... and the RNeasy Plus RNA Extraction Kit (Cat# 74134, Qiagen). Reverse transcription to generate cDNA was performed using TaqMan Reverse Transcriptase Kit according to the manufacturer’s instructions (Cat# N8080234 ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribosomal RNA was depleted with QIAseq FastSelect HMR kit (Qiagen). RNA was fragmented ...