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Citations for Qiagen :
551 - 600 of 951 citations for Small ribosomal subunit protein uS12 RPS23 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: Total protein was extracted from snap frozen tissues with a Tissue Lyser II (Qiagen) using 5mm Bead Beater balls (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... the proteins were purified by affinity chromatography with Ni-NTA superflow affinity column (Qiagen) as previously described (33).
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bioRxiv - Microbiology 2023Quote: ... The remaining protein mixtures were incubated with 50 μL Ni-NTA agarose beads (QIAGEN) at 4℃ for 1.5 h with constant rotation ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins harboring the His6 tag were captured by incubation with Ni- NTA resin (Qiagen) (2ml resin per bacterial lysate from 1L culture ...
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bioRxiv - Microbiology 2024Quote: ... The DENV2 EDIII and Halo-tag proteins were purified using Ni-NTA agarose (Qiagen) from the cell culture medium ...
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bioRxiv - Molecular Biology 2024Quote: Human CRM1 was expressed as His-tag fusion protein from a pQE60 plasmid (Qiagen) in E ...
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bioRxiv - Molecular Biology 2024Quote: ... Recombinant protein was captured by overnight incubation with 3 ml Ni-NTA resin (Qiagen) followed by washing with 30 ml denaturing buffer (8 M urea ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... RNA was extracted using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Cat. #80004) and stored at -80°C until use ...
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bioRxiv - Immunology 2024Quote: ... The recombinant proteins with His-tag were purified with Ni-NTA Agarose (30210, Qiagen). The proteins loaded onto the Ni-NTA column were washed with 60 column volumes of the Buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted protein was incubated with 100 μl of Ni-NTA agarose beads (QIAGEN) (washed and preincubated with 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein in the soluble lysate was bound to 8 mL Ni-NTA resin (Qiagen), washed with 10 mL low salt wash buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Protein in the soluble lysate was bound to 1 mL Ni-NTA resin (Qiagen), washed with 5 mL low salt wash buffer (20 mM Tris HCl pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... the proteins were purified were purified with the Strep-tactin Superflow Plus resin (Qiagen) and eluted by cleavage with desthiobiotin ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed at indicated time points after warming and then processed for immuno-identification of HPK using an antibody that recognizes the polyhistidine tag (RGS-His antibody; Qiagen 1:100) and counterstained for nuclei using DAPI ...
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bioRxiv - Biochemistry 2021Quote: ... and anti-His is a peroxidase-conjugated antibody (Qiagen). The membranes were incubated for 1 h at room temperature with horseradish peroxidase-conjugated goat anti-rabbit IgG (Sigma ...
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bioRxiv - Synthetic Biology 2021Quote: ... or mouse anti-RGS-His antibody (Qiagen; cat # 34610) at 1:5000 dilution followed by goat anti-rabbit-HRP conjugate (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... mouse antibodies against the His-tag (1:1000, Qiagen), rabbit antibodies against the C-terminal region of the collagen VI α1 chain (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-penta-His antibody (34660; Lot# 136244018; Qiagen); mouse anti-Myc tag mAb ...
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bioRxiv - Microbiology 2020Quote: ... The anti-His antibody conjugated to horseradish peroxidase (Qiagen) was used at a dilution of 1:5,000 ...
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bioRxiv - Plant Biology 2022Quote: ... and NoFa-ICL was the Penta-His antibody (Qiagen) at 1:4000 in TBST ...
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bioRxiv - Immunology 2022Quote: ... then incubated with the primary antibody anti-His (Qiagen) at a concentration of 1:2500 in a solution of 3% non-fat milk in TBS-T overnight at 4 °C ...
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bioRxiv - Biophysics 2024Quote: ... were functionalized with biotinylated α-penta-His antibody (Qiagen, Hilden Germany or R&D Systems ...
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bioRxiv - Biophysics 2023Quote: ... Anti-Penta-His antibody was purchased from Qiagen (Germany).
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bioRxiv - Biophysics 2023Quote: ... The antibodies were anti-RGSHis (Qiagen, Cat. No. 34650) and peroxidase-conjugated anti-mouse antibody (Dako ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein was extracted using a modification of the Qiagen AllPrep DNA/RNA Mini Kit (Qiagen) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... CRY2 protein was isolated by Ni2+-nitrilotriacetic acid (Ni-NTA) affinity chromatography (Qiagen, Hilden, Germany) following elution with 50 mM Tris buffer pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The His-tag protein was purified by using a column of Ni-Sepharose 4B (Qiagen), followed by gel filtration using a Superdex 75 column (G.E ...
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bioRxiv - Systems Biology 2020Quote: ... Upstream regulation and protein networks were evaluated via Ingenuity Pathway Analysis (IPA, QIAGEN, Hilden, Germany) based on the DEPs in the plasma experiment ...
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bioRxiv - Microbiology 2021Quote: ... The OrhR-His6 fusion protein was purified to homogeneity using Ni-NTA Spin Columns (Qiagen) and dialyzed against binding buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were purified under native conditions by affinity chromatography using Ni–NTA agarose (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Plant Biology 2022Quote: ... and the recombinant protein was purified on Ni-NTA agarose following the manfacturer’s instructions (Qiagen). The eluate (3x 500 μl ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified from clarified lysate by affinity chromatography on Ni2+-nitrilotriaceta-agarose (Qiagen) in 20 mM Tris-HCl 500 mM NaCl pH 8.0 buffer and by size exclusion chromatography on Superdex 200 HL 16/600 column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: Protein purification was carried out using QIAExpressionist NiNTA purification kit according manufacturer’s instructions (Qiagen, Germany) and following the HIS purification under native conditions protocol ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted from fetal tissues using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen) and bisulfite conversion was carried out using EZ DNA Methylation Kits (Zymo Research) ...
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bioRxiv - Biochemistry 2021Quote: ... The protein solution was subsequently applied to a 5 ml Ni-NTA Superflow cartridge (Qiagen) which was washed with dialysis buffer supplemented with increasing concentrations of imidazole (10-250 mM) ...
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bioRxiv - Microbiology 2020Quote: ... The histidine-tagged proteins MprA and MprA* were purified using Ni-NTA Agarose beads (Qiagen) using standardized protocol(Chadha et al. ...
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bioRxiv - Microbiology 2020Quote: ... 6x Histagged proteins were purified from the crude extract using Ni-NTA agarose columns (Qiagen) according manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... NspS-6xHis protein was purified from the clarified supernatant using Ni-NTA Superflow resin (Qiagen) equilibrated in lysis buffer ...
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bioRxiv - Cancer Biology 2022Quote: Breast tumor tissues (PTs and IBTRs) were processed using the AllPrep DNA/RNA/Protein (Qiagen) protocol ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged CSF3R protein was enriched by nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen 30210) and further purified by size-exclusion chromatography on a Superdex 75 10/300 GL column (Cytiva 17517401 ...
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Structure and Neutralization Mechanism of a Human Antibody Targeting a Complex Epitope on Zika VirusbioRxiv - Microbiology 2022Quote: ... Recombinant Fab proteins were purified from the culture supernatant by nickel-nitrilotriacetic acid agarose (Qiagen). Recombinant mAbs were affinity purified by MabSelect resin (Cytiva ...
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bioRxiv - Neuroscience 2023Quote: ... The list of quantified proteins was uploaded into IPA software (Qiagen, Redwood City, CA, USA) for further analyses ...
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Phospho-KNL-1 recognition by a TPR domain targets the BUB-1–BUB-3 complex to C. elegans kinetochoresbioRxiv - Cell Biology 2024Quote: ... 6xHis tagged proteins were purified from the clarified lysates by using Ni-NTA beads (Qiagen), and incubated with end-over-end rotation overnight at 4°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... All proteins were purified by gravity flow Ni2+ -NTA affinity chromatography (Qiagen, Cat. -No. 30230), except TEV-construct which was purified by gravity flow Strep-Tactin®XT Superflow® high-capacity resin (IBA Cat ...
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bioRxiv - Biophysics 2024Quote: DR2539 truncated protein was initially screened by the following commercial crystallization screens: Classics Suite (Qiagen), Salt grid (Hampton Research) ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant lysates were purified on Ni-NTA agarose beads (for His6-tagged proteins, Qiagen) or Glutathione High Capacity Magnetic Agarode Beads (for GST-tagged proteins ...
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bioRxiv - Cell Biology 2023Quote: All constructs for bacterial protein expression were cloned in the pQE plasmid (Qiagen, Valencia, CA). WT and mutant TTC1 were cloned downstream of a His14-bdSUMO-GFP tag ...
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bioRxiv - Immunology 2023Quote: ... the proteins were purified by Ni-NTA affinity chromatography using Ni-NTA agarose beads (Qiagen) and gel filtration with a HiLoad 16/600 Superdex 75 pg column in buffers containing 20 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants containing soluble proteins were applied to a 5 mL NiNTA-agarose gravity column (Qiagen) pre-equilibrated with lysis buffer ...
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bioRxiv - Biophysics 2024Quote: ... The recombinant proteins were purified from the supernatants using Ni-NTA agarose affinity columns (Qiagen). The protein concentration was measured using the Bradford method (protein assay kit ...