Labshake search
Citations for Qiagen :
551 - 600 of 1254 citations for Protease Inhibitor Cocktail EDTA Free 100X in DMSO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... with on-column DNase digestion with the RNase-free DNase Set (Qiagen). RNA concentration and purity were evaluated using a NanoDrop One spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2019Quote: ... The RNeasy Mini Kit and RNase-free DNase set were from Qiagen. High-Capacity cDNA Reverse Transcription Kit was from Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... Extracted DNA was eluted in 75μl Microbial DNA-Free water (Qiagen GmbH). Suitable precautions were taken during sample handling and processing in the laboratory to limit microbial contamination and maintain consistency during all procedures ...
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bioRxiv - Cell Biology 2020Quote: ... which included a genomic DNA digestion step with RNase-free DNase (Qiagen), as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... and 2.5 μL of nuclease-free water containing predesigned primers (#249900, Qiagen; QuantiTect Primer Assays IL-6 ...
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bioRxiv - Pathology 2019Quote: ... following manufacturer’s protocol and eluted in 30 µl nuclease-free water (Qiagen). Final concentration and purity were measured using NanoDrop™ 2000 Spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Total RNA was isolated from the cells using the RNeasy mini isolation kit from Qiagen (Germany) with a purification step using the RNase-free DNase kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... The plasmid was purified with the Endo-Free Mega prep kit (Qiagen). Of note ...
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bioRxiv - Neuroscience 2019Quote: ... Purified RNA was inbcubated with DNase I (RNase-Free DNase Set, Qiagen) over a mini kit column (RNAeasy ...
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bioRxiv - Genetics 2020Quote: ... Total RNAs were treated with amplification-grade RNase-free DNase I (Qiagen) to remove contaminating DNAs ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μg RNA was DNase treated using RNase-Free DNase Set (Qiagen) and 1 μg of DNase treated RNA was then taken for cDNA synthesis using the Protoscript I first strand cDNA synthesis kit (New England Biolabs) ...
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bioRxiv - Biophysics 2020Quote: ... The cell-free extract was bound to pre-equilibrated Ni2+-NTA (Qiagen) matrix for 3 h ...
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bioRxiv - Cell Biology 2021Quote: ... with on-column DNase digestion with the RNase-Free DNase Set (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA samples were then treated with the RNase-free DNase set (Qiagen) to remove gDNA contaminants and QC checked using Qubit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... The extracted RNA was treated with RNase-free DNase set (Qiagen, USA) to remove residual genomic DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... with 15 minute on-column digestion with RNase-free DNase I (Qiagen) to eliminate genomic DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA samples were further treated with RNase-free DNase kit (Qiagen), reverse transcribed using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Cell Biology 2022Quote: DNA-free RNA was obtained with the RNeasy MiniKit (Qiagen, Valencia, CA) with DNase treatment ...
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bioRxiv - Microbiology 2022Quote: ... Residual DNA in RNA samples was digested with RNase-free DNase (Qiagen) I and cleaned three times on a Spin Column from PureLink® RNA Mini Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... On-column DNase I treatment using the RNase-free DNase set (Qiagen) was included in the RNA extraction to remove any DNA contaminants ...
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bioRxiv - Molecular Biology 2022Quote: ... The resultant RNA was then treated with RNase-free DNase Set (QIAGEN) according to the protocol provided by the manufacturer with some minor modifications ...
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bioRxiv - Plant Biology 2022Quote: ... Genomic DNA was removed from samples by RNase-free DNase I (Qiagen) treatment ...
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bioRxiv - Immunology 2022Quote: ... with an on-column DNase digestion using RNAse-free DNase Set (Qiagen) for 30 minutes to eliminate genomic DNA contamination ...
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bioRxiv - Neuroscience 2022Quote: ... and genomic DNA was removed using the RNase-Free DNase Set (QIAGEN). Retrotranscribed cDNA was obtained from 1 μg of total RNA using the SuperScript VILO Retrotranscription kit from Life Technologies according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... with on-column DNase digestion using RNase-Free DNase Set (QIAGEN 79254). cDNA was synthesized with random hexamers from the Superscript III First-Strand Synthesis System (Invitrogen 18080051) ...
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bioRxiv - Microbiology 2023Quote: ... frozen liquid samples using the phenol-free RNeasy Plus Mini Kit (Qiagen) according to the instructions of the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... DNase treatment was performed on-column using RNase-Free DNase Set (QIAGEN). cDNA was generated from 200ng-1ug RNA by using SuperScript™ IV VILO™ Master Mix with ezDNase™ Enzyme Kit (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... which included an in-line RNAse-free DNase removal step (Qiagen 79256). Total RNA was reverse transcribed to cDNA and amplified with V4-specific primers [39] ...
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bioRxiv - Immunology 2023Quote: ... The RNA was purified in 30 µL of nuclease-free water (Qiagen), and RNA concentration measured on a NanoDrop™ 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... including digestion of contaminating DNA using an RNase-free DNase set (QIAGEN). RNA was diluted from the columns in ultrapure distilled water ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was removed from samples using the RNase-free DNase Set (QIAGEN). RNA quantity and quality was assessed using the Agilent 2100 Bioanalyser in conjuction with the RNA 6000 PicoLabChip Kit following manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... All plasmids were purified using the Endotoxin-Free Plasmid Purification Kit (QIAGEN) prior to the in vivo gene-delivery experiment.
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bioRxiv - Cell Biology 2023Quote: ... and on-column DNase digestion with the RNAse-Free DNase Set (QIAGEN). Purity and concentration of the extracted RNA were measured using Nano-Drop lite Spectrometer (Thermo-Fisher ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and on-column DNA digestion with the RNase-free DNase set (Qiagen). In order to remove any remaining DNA contamination ...
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bioRxiv - Plant Biology 2023Quote: ... followed by DNase digestion with RNase free DNase Set (Qiagen, Hilden, Germany). RNA samples were quantified on Nanodrop (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... and included DNA digestion using RNAse-free DNAse kit (Qiagen, cat.: 79254). Total RNA was quantified by UV spectrometry at 260 nm and RNA purity was estimated by 260/230 and 260/280 absorbance ratios using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... with on-column DNA digestion using RNase-Free DNase Set (Qiagen, Germany). Purified RNA was quantified using QubitTM RNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: DNA constructs were prepared using the endotoxin-free Maxiprep kit (Qiagen, #12362), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... in RNase-free conditions following the user’s manual with DNase treatment (Qiagen). Quality control of the samples was done via Tapestation (Agilent Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was DNAse treated with RNAse-free DNAse kit (Qiagen, Milan, Italy) and it was further quantified using Nanodrop One spectrophotometer (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... RNA eluted in nuclease-free water was then treated with DNase (Qiagen) at room temperature for 25 min to remove genomic DNA ...
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bioRxiv - Genetics 2023Quote: ... DNA contamination was further removed by the RNase-Free DNase Set (QIAGEN). RNA libraries were prepared with Illumina TruSeq Stranded mRNA (poly-A selection ...
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bioRxiv - Neuroscience 2024Quote: ... All samples were treated with RNase Free DNase Set (Qiagen. The Netherlands) to remove genomic DNA contamination ...
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bioRxiv - Molecular Biology 2024Quote: ... The extracted RNA samples were treated with RNase-free DNase set (Qiagen). For RNA-seq ...
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bioRxiv - Genomics 2024Quote: ... Endotoxin-free plasmids were prepared using the EndoFree Plasmid Maxi Kit (QIAGEN) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was digested on column using RNase-Free DNase Set (Qiagen 79254). RNA was reverse transcribed using High-Capacity cDNA Reverse Transcription kit with RNase Inhibitor (Applied Biosystems 4374966 ...
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bioRxiv - Microbiology 2023Quote: ... Cell-free supernatant was lysed with AVL buffer (Qiagen cat. No 19073) and RNA was extracted with QIAmp Viral RNA Mini Kit (Qiagen 52904).
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bioRxiv - Molecular Biology 2019Quote: ... the TEV protease was removed using Ni-NTA Agarose (QIAgen, 30210) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... and 1 μL of 1 AU/mL protease (Qiagen cat # 19157) to each well followed by a 60 min incubation at 55 °C ...
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bioRxiv - Neuroscience 2020Quote: ... His-tagged TEV protease was removed using Ni-NTA agarose (Qiagen). For FRET experiments ...