Labshake search
Citations for Qiagen :
551 - 600 of 2361 citations for Methyl 2 trifluoromethylsulfonyloxy 1 naphthoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2022Quote: ... Aliquots of 2 mL of cell cultures were submitted to DNA extraction using DNeasy Blood & Tissue kit (QIAGEN). DNA samples were quality checked and genotyped using PCR to confirm strains (Table S5) ...
-
bioRxiv - Microbiology 2022Quote: ... Primary-pools of ants were homogenized with 2 ceramic beads (2.8mm) in 350 µl QIAzol Lysis Reagent (Qiagen) for 2×2min at 30Hz in a TissueLyser II (Qiagen) ...
-
bioRxiv - Molecular Biology 2024Quote: ... brucei genomic DNA was isolated from ∼ 2 × 107 bloodstream form cells using a DNeasy Blood & Tissue Kit (Qiagen) using standard methods.
-
bioRxiv - Molecular Biology 2024Quote: ... ∼80 uL packed irradiated embryos and control embryos were resuspended in 2 mL buffer G2 (Qiagen, catalog # 1014636) and digested with proteinase K (1 mg/mL ...
-
bioRxiv - Neuroscience 2024Quote: RNA was extracted from SH-SY5Y and SK-N-BE(2) cells using the RNeasy mini kit (Qiagen) following the manufacturer’s protocol including the on-column DNA digestion step ...
-
bioRxiv - Cancer Biology 2024Quote: RNA isolation from the MIA PaCa-2 clones was performed by using the RNeasy Plus Micro Kit (QIAGEN). Libraries for RNA-seq were made with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...
-
bioRxiv - Immunology 2024Quote: ... 2 × 104 cells were sorted in triplicate per sample and stored at −80°C in RLT buffer (Qiagen). RNA was isolated using the Qiagen RNeasy Micro Kit ...
-
bioRxiv - Cell Biology 2023Quote: Up to 2 μg RNA was reverse transcribed to cDNA using the Omniscript RT Kit (Qiagen, Manchester, UK) or High-Capacity Reverse Transcription Kit (ThermoFisher ...
-
bioRxiv - Genomics 2023Quote: ... the 2 pairs of bacteriomes were dissected and DNA was extracted using the DNEasy Blood & tissue Kit (Qiagen). DNA library was constructed using the NGS Nextera Flex DNA library preparation kit (Illumina) ...
-
bioRxiv - Developmental Biology 2022Quote: ... total RNA was extracted from 2-3 embryos pooled per genotype using the RNeasy Mini Kit (QIAGEN, 74106) and QIAshredder columns (QIAGEN ...
-
bioRxiv - Microbiology 2022Quote: ... using a MiSeq reagent kit v3 with 2 × 75 paired end-reads and sequence analysis was performed using CLC-Genomics workbench (v10.1.1., Qiagen). Enrichment in reads of each sample was compared to the corresponding control and mapped as peaks against the annotated genome of S ...
-
bioRxiv - Microbiology 2022Quote: ... Viral RNA was extracted from 140 µl of virus stock (SARS-CoV-2 VOC Delta, GISAID ID: EPI_ISL_15250227) using a QIAamp Viral RNA Mini Kit (Qiagen) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... coli grown for 16 h in 250 ml of 2×YT medium using the Plasmid Maxi Kit (QIAGEN) in accordance with the manufacturer’s protocol ...
-
bioRxiv - Physiology 2022Quote: ... approximately 100 mg of adipose tissue and a 7 mm metal bead were placed in a 2 mL centrifuge tube containing 0.5 mL PBS buffer (pH 7.4) and homogenised in TissueLyser II (Qiagen) for 2 minutes at 25 Hz ...
-
bioRxiv - Neuroscience 2023Quote: ... Tissue was placed into 2-mL round-bottom homogenizer tubes pre-loaded with Stainless Steel beads (#69989; Qiagen) and filled up with lysis buffer (#AA-LYS-16ml ...
-
bioRxiv - Immunology 2023Quote: ... total RNA was extracted from proximal colonic tissues (2 mm) using RNeasy Mini Kit with DNase treatment (QIAGEN), and synthesis of cDNA was conducted with High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher ...
-
bioRxiv - Biochemistry 2023Quote: ... brucei genomic DNA was isolated from ∼2 × 108 bloodstream form cells using a DNeasy Blood & Tissue Kit (Qiagen).
-
bioRxiv - Developmental Biology 2023Quote: ... with the addition of a 2-minute spin at 15,000 x g in the QIAshredder (Qiagen, Germantown, MD) prior to the genomic DNA elimination step ...
-
bioRxiv - Genomics 2024Quote: ... Amplicons were generated in 2 different 25 µL PCR reactions (9.5 µL nuclease-free water, 12.5 µL 2x QIAGEN Multiplex PCR Master Mix ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 2 µg of gDNA was used for bisulfite conversion using the Epitect bisulfite conversion kit (QIAGEN 59124) or 200 ng gDNA was used for enzymatic conversion (NEB E7125) ...
-
bioRxiv - Developmental Biology 2024Quote: ... 200 ng psiCHECK-2 reporter plasmid were co-transfected with 5 µl miScript miRNA mimics (Qiagen; CatNo. 219600) using 4 µl Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... containing 2-mercaptoethanol (1µL/100µL RLT) and total RNA was isolated using the RNeasy Plus Micro Kit (Qiagen). RNA concentration was determined with the NanopDrop One spectrophotometer (NanoDrop ...
-
bioRxiv - Biophysics 2024Quote: ... for 4 hours at 37°C and purified from a 2% agarose gel (QIAquick Gel Extraction Kit, Qiagen). At this stage ...
-
bioRxiv - Biophysics 2024Quote: ... The supernatant was the incubated at 4 °C with 2 ml of washed Ni-NTA agarose beads (Qiagen) for 1 hour ...
-
bioRxiv - Genomics 2020Quote: ... 1 ul (1U/ul) UDG and 1 ul RNaseA (100 mg/ml, Qiagen) and reactions were incubated at 37°C for 3 hrs ...
-
bioRxiv - Genomics 2023Quote: ... gracilis (1 male and 1 female) using the Rneasy Mini Plant Kit (Qiagen) following the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2021Quote: ... The solubilized fraction was then incubated for 2 h with 300 µL of pre-equilibrated Ni-NTA resin (QIAGEN) in Ni-NTA buffer (50 mM Tris-HCl ...
-
bioRxiv - Biophysics 2021Quote: cfDNA was isolated from 2 mL of plasma for each sample via the QIAamp Circulating Nucleic Acid kit (Qiagen). ddPCR was performed using the QX200 Droplet Digital PCR System (Bio-Rad) ...
-
bioRxiv - Developmental Biology 2021Quote: ... the second PCR used 2 µL of the first reaction as template and HotStarTaq DNA Polymerase (Qiagen, Hilden, Germany) in a 20 µL reaction volume for 15 cycles ...
-
bioRxiv - Immunology 2021Quote: ... RNA of cells exposed to SARS-CoV-2 WT was isolated with the QIAamp Viral RNA Mini Kit (Qiagen) according to the manufacturer’s protocol ...
-
bioRxiv - Evolutionary Biology 2020Quote: DNA was extracted from the exterior (outermost 2 mm) tissue and water filters using the DNeasy PowerSoil Kit (Qiagen) and from the interior tissue (at least 2 mm away from the outermost edge of the specimen ...
-
bioRxiv - Neuroscience 2022Quote: RNA was isolated from snap frozen striatal mouse tissue from 2 independent cohorts of mice according to the manufacturer’s protocol and purified using RNeasy columns (Qiagen). A total of 13 mRNA libraries for Illumina sequencing were prepared using KAPA mRNA HyperPrep Kit (KAPA Biosystems ...
-
bioRxiv - Immunology 2022Quote: ... in vitro generated PCs were sorted at day 2 and RNA were extracted with “RNeasy plus micro kit” (Qiagen) as recommended ...
-
bioRxiv - Genomics 2020Quote: ... and RNA was eluted using DTT (100mM, 2 × 100μL) and collected into 700μL RLT buffer from the RNeasy MinElute Cleanup Kit (Qiagen 74204). RNA was isolated using the RNeasy MinElute kit as per manufacturer’s instructions and was quantified using the Agilent Tapestation High Sensitivity RNA kit (Agilent 067-5576) ...
-
bioRxiv - Neuroscience 2020Quote: ... each individual cell was transferred to a 0.2 ml PCR tube containing 2 μl of lysis buffer (RLT Plus - Qiagen). The tube was immediately placed on a metal plate sitting on top of dry ice for flash-freezing ...
-
bioRxiv - Biophysics 2021Quote: The SARS-CoV-2 RNA was isolated from virus particles with the QIAmp viral RNA mini kit (Qiagen - USA) and reversely transcribed to cDNA with the High-Capacity Reverse Transcription Kit (Thermo - USA) ...
-
bioRxiv - Immunology 2021Quote: ... was then added to a polymerase chain reaction (PCR) mix containing 12.5 μl of 2× Multiplex PCR mix (Qiagen), 9 μl of H2O ...
-
bioRxiv - Cell Biology 2021Quote: Meta-analysis of the DESeq2 analysed RNA sequencing data was performed to identify the top pathways that are statistically associated with the list of the significantly induced genes77 (fold change > 2, FDR q value < 0.05, using the Ingenuity Pathway Analysis platform IPA, Ingenuity Systems, Qiagen).
-
bioRxiv - Cell Biology 2021Quote: Total RNA extracts were prepared from a minimum of 2×104 freshly sorted cells using RNeasy Micro Kit (Qiagen) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... 2 μg of microRNA was reverse transcribed using miScript Ⅱ microRNA cDNA Synthesis Kit (QIAGEN Biotechnology Co, Ltd, China). The reverse transcription reaction system included 4 μL of 5×miScript HiFlex Buffer ...
-
bioRxiv - Microbiology 2021Quote: The QIAseq SARS-CoV-2 Primer Panel paired with the QIAseq FX DNA Library construction kits (Qiagen GmbH, Germany) were used for enriching and sequencing the entire SARS-CoV-2 viral genome ...
-
bioRxiv - Molecular Biology 2020Quote: ... was added to the embryos and homogenized for 2 min at 40 Hz using a Tissue Lyser LT (Qiagen) and 5 mm stainless steel beads (Qiagen) ...
-
bioRxiv - Microbiology 2021Quote: Total RNA was extracted from supernatants of SARS-CoV-2-infected Vero E6 cells using the RNeasy kit (Qiagen) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from 2 g powdered clay samples using the PowerMax Soil DNA Isolation Kit (Qiagen, Germany) and modifications to the manufacturer’s instructions previously validated (22) ...
-
bioRxiv - Microbiology 2022Quote: ... One volume of bacterial culture containing approximately 0.2 OD600 of cells was mixed with 2 volumes of RNAprotect (Qiagen) and processed according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: Purified ZIKV RNA and HSV-2 DNA were extracted using QIAamp MinElute Virus Spin Kit (Qiagen, catalog no. 57704), and 50ng purified viral RNA/DNA was incubated with the recombinant protein at different concentrations for 90min at 37°C in the presence of SUPERase In™ RNase inhibitor (Invitrogen ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were mechanically disrupted by bead-beating for 2 × 3 min at 30 Hz (TissueLyser II, Qiagen, Hilden, Germany). The samples were incubated at −80°C for 10 min and at 95°C for 10 min ...
-
bioRxiv - Microbiology 2020Quote: ... 5 μl of cDNA was then added to a PCR mix containing 12.5 μl 2× Multiplex PCR mix (Qiagen), 9 μl H2O ...
-
bioRxiv - Biochemistry 2020Quote: ... Nanobody PCR fragments were analyzed on 2% TAE-agarose gels and purified with the QIAquick gel extraction kit (Qiagen). PCR fragments were sequenced and individual nanobodies were cloned using the In-Fusion cloning kit (Takara Bio ...
-
bioRxiv - Cancer Biology 2021Quote: ... The products were run on 2% agarose gel and extracted using the QIAquick Gel Extraction Kit (Qiagen, Cat#28706). The purified products were submitted for paired-end 300 bp sequencing on MiSeq (Illumina) ...