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Citations for Qiagen :
551 - 600 of 2211 citations for L Phenylalanine N 1 oxo 4 1 pyrenyl butyl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Genomic DNA from ∼250,000 neurons per sample (n = 3 samples per group) was extracted and purified (DNeasy Blood and Tissue DNA extraction kit, Qiagen) prior to RRBS (Ovation RRBS Methyl-Seq System ...
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bioRxiv - Biophysics 2020Quote: ... All the constructs also contain a His6-tag at the N-terminus for affinity purification with Nickel-NTA agarose beads (Qiagen). Site-directed mutagenesis was performed using the QuickChange Lightning kit (Agilent Technology) ...
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bioRxiv - Bioengineering 2022Quote: ... RNA was isolated from 3D samples (n=2-3) by combining a TRIzol-based cell lysis with a RNeasy Mini Kit (Qiagen). Samples were collected at aforementioned timepoints ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant protein fragments were produced in Escherichia coli (strain M15) as fusion products with an N-terminal tag of six histidines using plasmid pQE30 (Qiagen) and purified from total bacterial lysates by nickel affinity chromatography ...
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bioRxiv - Pathology 2022Quote: ... The N gene-specific primers were used to amplify 97 bp of SARS-CoV-2 N gene by conventional PCR and purified by Qiagen gel extraction kit (Qiagen ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was isolated from single-cell clone or bulk GFP+ and GFP- cells (n = 3 with cells from three different mice in each group) using QIAzol lysis reagent (Qiagen). DNA was removed using the RNase-Free DNase Set (QIAGEN) ...
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bioRxiv - Developmental Biology 2021Quote: ... total RNA was extracted from control and INPP5ED477N/D477N organoids (n=3 samples per genotype) using an RNeasy Plus Micro Kit (Qiagen) and reverse transcribed using Superscript™ IV VILO™ Master ezDNase enzyme (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... Total RNA was extracted from individual filter sets (n = 2 per timepoint) using Qiagen RNeasy Mini Kit (Qiagen, Hilden, Germany) as in Harke et al ...
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bioRxiv - Biophysics 2020Quote: ... Cells were transiently transfected with cDNA encoding the mouse Piezo1 channel or its mutants tagged with GFP on its N-terminus in the pCDNA3 vector using the Effectene reagent (QIAGEN). Cells were then trypsinized and re-plated on poly-D-lysine-coated round coverslips 24 hours after transfection ...
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bioRxiv - Microbiology 2022Quote: ... and digested DNA products were extracted from the agarose gel using the QIAquick Gel Extraction kit (QIAGEN, cat. n° 28704), according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: ... Cell debris were removed by centrifugation (35,000 g at 4°C for 20 min) and the N-terminal His-tagged proteins were purified from the supernatant using NiNTA agarose (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was extracted and purified from pooled scrambled control and genotyped cldn7b F0 knockout larvae (n=50 per group) at 7dpf using the RNeasy Plus Micro Kit (QIAGEN), followed by cDNA synthesis with the SuperScript IV VILO Master Mix Kit (ThermoFisher) ...
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bioRxiv - Immunology 2021Quote: ... These plasmids were purified and DNA extracted using QIAGEN Plasmid Midi Kit (100) (cat. n° 12145, QIAGEN, Valencia, CA, USA), according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: E9.5 hindbrain spanning rhombomeres 1-6 were dissected from wild type and mutant embryos (n=3) to obtain total mRNA preparations (miRNeasy Micro Kit, Qiagen). Sequencing libraries were prepared following the SMART-Seq v4 Ultra Low Input RNA (TaKaRa ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 21 days following X-ray exposure we extracted the total RNA (RNeasy® mini kit, Qiagen, cat. n. 74104) from 3 sponges for each treatment and control ...
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bioRxiv - Microbiology 2021Quote: ... coli giant spheroplasts we used a N-terminally 6-His tagged version of TcMscS cloned into the expression plasmid pQE80 (Qiagen) with restriction sites BamHI and HindIII ...
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bioRxiv - Microbiology 2020Quote: ... recombinant proteins were then separated from the His-tagged TEV protease (and the cut N-terminal portion) by gravity-flow separation on Ni-NTA agarose (Qiagen). Finally ...
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bioRxiv - Genetics 2020Quote: ... The supernatant of the transfected cells encoding for N-HIS-FAM19A5 was collected for Ni-NTA affinity chromatography (Qiagen, Germany). Purified N-HIS-FAM19A5 was then digested overnight at 30°C with AcTEV protease (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... membranes were solubilized in 1.2% N-decyl-β-maltoside (DM) and the protein was purified on a Ni-NTA affinity column (Qiagen). Following His-tag removal ...
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bioRxiv - Cell Biology 2022Quote: RNA was extracted from cryo-pulverized inguinal adipose tissue from freely-fed 14-day-old mice (n=3 male and female for PTPN23H/H and PTPN23+/+) using the RNeasy Mini Kit (74104; Qiagen) according to manufacturer’s instructions ...
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Single-cell assessment of trophoblast stem cell-based organoids as human placenta-modeling platformsbioRxiv - Developmental Biology 2022Quote: ... The methylation status of each CpG of interest (n=6 per sample) was evaluated with PyroMark Q-CpG software (Qiagen) and data from all CpG sites was averaged for each sample and presented as percent methylation (Figure S1E).
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bioRxiv - Genomics 2022Quote: Genomic DNA was extracted from all F2 individuals (n = 118) and their parental lines using the DNeasy Plant Mini Kit (Qiagen). The obtained DNA samples were digested with PstI and MspI to construct a double digest restriction-site associated DNA sequencing (ddRAD-Seq ...
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bioRxiv - Biochemistry 2024Quote: The periplasmically located VC0430 that was used for ESI-MS analysis was purified by applying the clarified lysate to N-NTA resin (Qiagen), washing the resin with Wash Buffer (WB ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted in random order from the faecal slurries (n=484) using DNeasy PowerLyzer PowerSoil kit (Qiagen, 12855-100) and the V3-region of the 16S rRNA gene was PCR amplified using 0.2 µl Phusion High-Fidelity DNA polymerase (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... total RNA was extracted from newly acquired human embryonic/fetal brain samples (n=32) using the AllPrep DNA/RNA Mini Kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: Total RNA was isolated from untreated or RNP-transfected plerixafor-mobilized HD and SCD HSPCs (n=3 for each group) using the RNeasy Kit (QIAGEN) that includes a DNAse treatment step ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was extracted from paired ovaries (N=6 per condition) using a miRNeasy micro extraction kit per the manufacturer’s instructions (Qiagen, 217084). RNA concentration and quality were assessed using the RNA Total RNA Nano Assay (Agilent Technologies) ...
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bioRxiv - Plant Biology 2023Quote: Genomic DNA (gDNA) was extracted from all samples using the QIAGEN DNEasy Plant Mini Kit (Qiagen N. V., Hilden, Germany) following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: We extracted total RNA from liver and BAT from each sample (n = ∼6 per genotype/sex/treatment/tissue) using the RNeasy PowerLyzer Kit (QIAGEN). We generated Illumina cDNA libraries from 1 μg of purified RNA using KAPA Stranded mRNA-Seq Kit (Illumina) ...
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bioRxiv - Physiology 2023Quote: DNA was extracted from fecal pellets (100 mg, n=5 per group) by the QIAmp Power Fecal DNA kit (12850-50, Qiagen). The V4 region of 16S rRNA gene was amplified ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from Day 11 populations (n=20, 5/treatment) using Qiagen DNeasy Blood and Tissue Kit (Qiagen, Germany) adapted from the manufacturer’s instructions to include a lysostaphin lysis step(50) ...
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bioRxiv - Neuroscience 2024Quote: Isolated dorsal striatal microglia (n = 5-7 per condition) were centrifuged for 5 min at 600xg and resuspended in RLT plus buffer (Qiagen) for extraction and purification of total RNA ...
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bioRxiv - Developmental Biology 2024Quote: ... meioc cDNA encoding 356 amino acid residues from N-terminus and ythdc2 cDNA encoding amino acid residue Arg743 to Leu1381 were cloned into a pQE-30 vector (QIAGEN) and a pET-21a (+ ...
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bioRxiv - Microbiology 2024Quote: ... were prepared from each rumen digesta sample (n=24) as follows: samples were homogenized to a fine powder using a tissue lyser (Qiagen). Approximately five volumes of 70% ethanol were added ...
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bioRxiv - Biochemistry 2024Quote: ... laevis STK19 variants expressed with a PreScission protease cleavable N-terminal His6 tag were purified using Ni-NTA Superflow resin (Qiagen) equilibrated in STK19 NiNTA Buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Genetics 2024Quote: ... A total of l µg plasmids were transfected using the Effectene transfection reagent (Qiagen, 30l425). Three days after transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... and clarified lysate was incubated with 1 ml packed Ni-NTA resin (QIAGEN) per 750 ml culture for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The cleared extract was loaded onto 1 ml pre-equilibrated NiNTA resin (Qiagen). The column was washed extensively with Nickel buffer (25 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... with 1% 2-Mercaptoethanol (Serva) and disrupted using the Qiagen Tissue Ruptor (Qiagen). Total RNA was extracted using the RNeasy Fibrous Tissue Mini Kit (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μg of genomic DNA extracted using the DNeasy Blood & Tissue kit(Qiagen) was bisulfite-converted by using the EZ DNA Methylation kit (Zymo Research) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the plugs were treated with RNase (Qiagen, 20µl in 1 ml TE buffer) for 1h at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... bron-1 and bron-2 root tips using the RNeasy Micro Kit (Qiagen). qPCR was performed with SYBR green (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... weighed and homogenized in 1 ml of DMEM with stainless steel beads (QIAGEN) for 30s at 30 Hz using TissueLyserII (QIAGEN) ...
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bioRxiv - Microbiology 2020Quote: ... Cell lysate was mixed with 1 mL of Ni-NTA agarose beads (Qiagen), and the mixture was rotated at 4 °C for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg of total RNA was retrotranscribed using QuantiTect Reverse Transcription kit (Qiagen). Primers designed to amplify cpxP ...
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bioRxiv - Neuroscience 2020Quote: Antennae from twenty moths were homogenized in 1 mL QIAzol (Qiagen, Hilden, Germany). RNA was extracted using Qiagen RNeasy Mini kit (Qiagen ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg of total RNA extracted with the RNeasy Plant Mini Kit (Qiagen) was treated with DNaseI (Ambion ...
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bioRxiv - Microbiology 2020Quote: ... The lysates were then incubated with 1 mL prewashed Ni-NTA resin (Qiagen) at 4 °C for 2 h on a rotator ...
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bioRxiv - Microbiology 2020Quote: ... The cell pellets were resuspended in 1 ml Qiagen RNA protect reagent (Qiagen) and incubated for 24 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: 2 □ 107 spores were mixed with 1 ml RNAlater RNA stabilization reagent (Qiagen) and centrifuged at 17,000 g for 5 min ...