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Citations for Qiagen :
551 - 600 of 1597 citations for Insulin like growth factor binding protein 6 IGFBP 6 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... and the Tbx6VE+ and Tbx6VE- fraction was sorted in 1.5ml low-binding tubes with 350μl RLT (Qiagen) + 1% v/v B-mercaptoethanol (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... the list of binding partners was annotated using Ingenuity Pathway Analysis (IPA, www.qiagen.com/ingenuity; Qiagen; March 2019) tool.
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bioRxiv - Molecular Biology 2023Quote: ... bead-bound DNA was washed twice with cold binding buffer and three times with EB buffer (Qiagen).
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bioRxiv - Biophysics 2021Quote: ... Media containing his-tagged Ecads was passed through a chromatography column containing Ni-NTA agarose beads (Qiagen). Beads were then washed with a pH 7.5 biotinylation buffer (25mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... Media containing his-tagged Ecads was passed through a chromatography column containing Ni-NTA agarose beads (Qiagen). Beads were then washed with a pH 7.5 biotinylation buffer (25 mM HEPES ...
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bioRxiv - Molecular Biology 2022Quote: ... The JAK3 PCR fragment was purified and cloned into the pQE-Tri System His-Strep2 vector (Qiagen). The final pQE-JAK3 construct was used to transfect GC using the Xfect transfection kit (Takara Bio ...
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bioRxiv - Microbiology 2019Quote: ... and Western blot analysis was performed as previously described (30) using a primary anti-his antibody (Qiagen) and a secondary Alexa Fluor 700 goat anti-mouse antibody ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was mixed with 1 ml of 50% Ni2+-NTA His-bind resin (Qiagen, Hilden, Germany), incubated for 1 hr at 4°C with mild rotatory shaking ...
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bioRxiv - Synthetic Biology 2020Quote: ... The plates were coated with 100 μL of 4 μg/mL murine anti-His mAb (Qiagen, #34660), and after blocking ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged Sic1PY conjugates (polyubiquitylated Sic1PY, Ubn-Sic1PY) were purified by incubating with Ni-NTA resin (Qiagen) at 4 °C for 1 h ...
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bioRxiv - Bioengineering 2023Quote: ... Purification of His-tagged GA-MatryoshCaMP6s was performed with Ni-NTA agarose beads (Qiagen, cat. No: 30210). Before applying the lysate ...
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bioRxiv - Biochemistry 2020Quote: ... coli* culture from each growth condition were stabilised in RNAProtect Bacteria Reagent (2 mL; Qiagen) and cells pellets were frozen at −80°C prior to processing ...
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bioRxiv - Biophysics 2019Quote: ... and plasmids were harvested from this growth using a Qiafilter Plasmid Midi Kit from Qiagen Inc ...
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bioRxiv - Genomics 2019Quote: ... The mixture was then passed through the RNA binding column of RNeasy Mini Kit (74104, Qiagen, Germantown, MD), followed by washing and elution steps described in the manufacture’s handbook.
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bioRxiv - Biochemistry 2020Quote: ... DNA constructs were amplified in Escherichia coli DH5α and purified using a Hi-Speed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Biochemistry 2020Quote: ... (2010) using commercial antibodies directed against the His-tag following the instructions of the manufacture (Qiagen, Hilden, Germany).
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SARS-CoV-2 comprehensive receptor profiling: mechanistic insight to drive new therapeutic strategiesbioRxiv - Cell Biology 2021Quote: ... each pre-incubated at a 2:1 molar ratio with Penta His Alexa Fluor 647 Conjugate (Qiagen, UK). Hits (duplicate AF647 positive spots ...
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bioRxiv - Microbiology 2020Quote: A 6.5 nM concentration of His-tagged 16055 SOSIP.v8.3 trimer in TBS was added to a 96-well Ni-NTA plate (Qiagen) for 2 h at RT ...
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bioRxiv - Biochemistry 2019Quote: ... DNA constructs were amplified in Escherichia coli DH5α and purified using a Hi-Speed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Microbiology 2020Quote: ... His-SFP1 was purified using Ni-NTA affinity purification under native conditions using the standard manufacturer’s protocol (Qiagen). Recombinant His-SFP1 was used to immunize female New Zealand white rabbits (Covalab ...
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bioRxiv - Biochemistry 2021Quote: DNA constructs were amplified in Escherichia coli DH5α and purified using a Hi-Speed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Biochemistry 2022Quote: ... Lysates of His-tagged talin constructs were purified by Ni-NTA-sepharose affinity chromatography (Ni2+-nitrilotriacetic acid, Qiagen), followed by a gel filtration column (Superdex 200 ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates of His-tagged vinculin constructs were purified by Ni-NTA-sepharose affinity chromatography (Ni2+-nitrilotriacetic acid, Qiagen), followed by a Q-Sepharose ion exchange column ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% CA630) and rotated at 4°C for 2 hours before addition of His-beads (Ni-NTA, QIAGEN). After continuous rotating for another 2 hours ...
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bioRxiv - Plant Biology 2024Quote: ... All fractions were analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting using anti-His (Qiagen) and anti-strep (MilliporeSigma ...
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bioRxiv - Microbiology 2020Quote: Aliquots taken from broth growth were immediately added to two volumes of RNAprotect Bacteria Reagent (Qiagen) for immediate stabilization of bacterial RNA ...
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bioRxiv - Microbiology 2020Quote: RNA was extracted from T cells in their exponential growth phase using QIAshredder (Qiagen, Cat# 79654) and RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Genetics 2022Quote: Total RNA was extracted in the logarithmic growth phase using the RNeasy RNA isolation kit (Qiagen). Synthesis of complementary DNA was performed using QuantiTect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... prior to growth inhibition assays and genomic DNA (gDNA) extraction (DNeasy Blood and Tissue kit (Qiagen).
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bioRxiv - Cancer Biology 2021Quote: ... Individual tumors were collected in low binding DNA tubes and digested in 3 μl RLT buffer (Qiagen Cat# 1048449) for 30min at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... clarified samples were transferred to a 50 mL conical tube and supplemented with a final 20 mM imidazole pH 7.5 before batch-binding with Ni-NTA superflow resin (Qiagen) for ∼1 hr at 4°C ...
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bioRxiv - Genetics 2021Quote: ... followed by protein precipitation with the Protein Precipitation Solution (Qiagen) for 10 min at -20 °C ...
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bioRxiv - Biophysics 2021Quote: ... gliding motility assays were performed with specific immobilization of motors on the surface via penta-His antibodies (34660, Qiagen) as previously described[64] (Figure S4) ...
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bioRxiv - Molecular Biology 2022Quote: ... or Renilla-HA-NOD2 (750 ng/well) and His-FLAG-CAD (250 ng/well) using Effectene Transfection Reagent (Qiagen). The following day ...
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bioRxiv - Microbiology 2021Quote: ... His-N-WASP ± MirA was loaded onto a column with 80 µL of previously equilibrated Ni-NTA resin (Qiagen) and incubated for 1 h at 4°C with agitation ...
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bioRxiv - Molecular Biology 2022Quote: His-Prs or its variants (10 µg) were bound to 4 µl of Ni-NTA Magnetic Agarose Beads (Qiagen) in 50 µl interaction buffer (50 mM Tris pH 9.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Expression levels were validated from the pWB plasmid by western blot using an anti-His HRP conjugate kit (Qiagen) (Fig ...
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bioRxiv - Immunology 2020Quote: ... ConM-SOSIP.v7 carrying a C-terminal His-tag (diluted to 6.5nM in TBS) was immobilized onto 96-well Ni-NTA ELISA plates (Qiagen) by incubation for 2 hours at room temperature after which the plates were washed 3 times with TBS ...
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bioRxiv - Bioengineering 2020Quote: ... the membrane was incubated with freshly prepared primary antibody solution (1:1,000 dilution for anti-Alix [ab117600, Abcam], anti-Tsg101 [ab30871, Abcam], anti-Calnexin [ab22595, Abcam], anti-His [34660, Qiagen] ...
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bioRxiv - Microbiology 2019Quote: ... Total RNA was isolated from log-phase bacterial cells grown in the rat peritoneal cavity or in 2.5% NaCl HI broth using the RNeasy minikit (Qiagen). One microgram of purified RNA was converted into cDNA using QuantiTect® Reverse Transcription Kit (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... The filtrated supernatant containing His-thioredoxin-ApoE was applied to a 5 ml Ni-NTA Superflow Cartridge (Qiagen, Germany) pre-equilibrated with TBS buffer A ...
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bioRxiv - Plant Biology 2023Quote: ... The His and GST tag were cleaved by the Tev protease and separated from AtPAXXL228E using Ni-NTA (Qiagen). AtPAXXL228E was loaded onto a HiTrap Q HP column equilibrated with 20 mM Tris pH7.4 ...
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bioRxiv - Genomics 2023Quote: ... we first extracted nuclei from fresh leaves and then prepared libraries using the EpiTect Hi-C Kit (Qiagen, Netherlands). HiC libraries were sequenced on a NextSeq500 instrument (Illumina ...
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bioRxiv - Biochemistry 2023Quote: ... The filtrated supernatant containing His-tagged NanoLuc was applied to a 5-mL Ni-NTA Superflow Cartridge (Qiagen, Germany) pre-equilibrated with TBS buffer A ...
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bioRxiv - Neuroscience 2019Quote: ... 200 μl of ethanol was added to the samples prior to binding to Qlamp spin column (QIAamp DNA Blood mini kit; Qiagen) and samples were centrifuged at 8000 rpm for 1 minute ...
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bioRxiv - Systems Biology 2020Quote: ... and RNA seqFISH primary probe pools (1-10 nM per probe) and 10 nM polyT LNA oligo with a readout probe binding DNA sequence (Qiagen) were hybridized in 50% hybridization buffer consisted of 50% formamide (Invitrogen AM9342) ...
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bioRxiv - Microbiology 2020Quote: ... VSG2-antibody negative clones (positive transfectants were identified by lack of binding) were sequenced: RNA was isolated using the RNeasy Mini Kit including an on-column DNase treatment (Qiagen). Complementary DNA was synthesized with SuperScript IV Reverse Transcriptase (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... homogenized as described in the ‘RNA sequencing’ section were passed through the RNA binding column from the AllPrep DNA/RNA Mini kit (Qiagen) and the flow through was collected for protein precipitation ...
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bioRxiv - Biochemistry 2022Quote: ... The peptide spot membrane was blotted onto a nitrocellulose membrane and UNC-45 binding was probed with an anti-Strep antibody (Qiagen) using an ECL-kit for detection (GE Healthcare).
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bioRxiv - Evolutionary Biology 2023Quote: ... The remaining supernatant was mixed with 13x its volume of binding buffer and DNA was purified with MinElute columns (Qiagen), following the manufacturer’s instructions with the exception of a 15-minute incubation at 37□°C during the elution step ...