Labshake search
Citations for Qiagen :
551 - 600 of 1739 citations for Genz 644282 CAS 529488 28 6 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... was extracted and purified from pools of 30 zebrafish larvae at 6 dpf using the RNeasy Plus Mini Kit (Qiagen, Germantown ...
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bioRxiv - Genomics 2024Quote: ... Each sample was mixed with 5 µL of one of 6 Illumina adapter sequences (specified in the Qiagen protocol appendix) and 2 µL of ultralow input ligase ...
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bioRxiv - Neuroscience 2024Quote: ... in vitro hTAM were incubated for 6 hours at 37°C with predesigned siRNA against human SPP1 (Qiagen, cat.#1027416) or Scramble (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... After 6 h RNA was extracted and1 µg of total RNA was used for reverse transcription (Quantitect reverse transcription kit, Qiagen) as per instructions ...
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bioRxiv - Microbiology 2024Quote: ... 6×105 cells were seeded onto type I collagen-coated 6-well plates and transfected with 200 ng of viral DNA using Attractene Transfection Reagent (Qiagen). Two days later ...
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bioRxiv - Physiology 2024Quote: ... from TKOc and CTRL mice and livers of 3-month-old young and 18-month-old aged C57BL6/JN mice (n=6 per group, equally distributed males and females, total n=96 libraries) using a QIACube (Qiagen). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was isolated from mouse prostate cell lines or WT prostate tissue from 8-10 week old C57BL/6 mice using the RNeasy Mini Kit (Qiagen). Complementary DNA (cDNA ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted complex was then mixed to 6 μL of 5 M NaCl and 2 μL of 100 mg/mL RNase A (Qiagen) and incubated overnight at 65°C to reverse cross-linking and digest RNA ...
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bioRxiv - Genetics 2024Quote: ... RNA was isolated from cultures incubated or not with 1 µM staurosporine for 6 hours to induce apoptosis using the RNeasy Mini Kit (QIAGEN). RNA samples were checked for quality on Agilent 4200 TapeStation System (Agilent Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was extracted from whole brain of 6 dpf larval zebrafish using RNeasy micro plus RNA extraction kit (Qiagen, Germany). Total RNA (2ng/µl ...
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bioRxiv - Genetics 2020Quote: ... and bands at 700 bp (insert) or 6 kb (plasmid library) cut and purified using QiaQuick Gel Extraction Kit (Qiagen #28706) with elution into 30 µL EB.
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bioRxiv - Evolutionary Biology 2021Quote: ... 2.5×106 cells were seeded per well in 6-well plates and transfected using Effectene transfection reagent (Qiagen, Cat. No. 301425). The transfection mixtures in Figure 5A contained 1 μg ...
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bioRxiv - Bioengineering 2021Quote: ... total RNA from the fibroblasts cultured with 10 μM Dwn1 was extracted every 6 hours from hours 24 to 48 (RNeasy® Plus Mini kit, Qiagen), followed by cDNA synthesis (SuperScript® VILO™ cDNA Synthesis Kit ...
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA from untreated cells (24 h) and IFNγ-treated cells (6 or 24 h) were extracted with RNeasy plus mini kit (Qiagen, 74134) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: E3 and E4 primary microglia were plated at 5×106 cells/well in 6-well plates and RNA was extracted from the cells using the RNEasy Plus Mini Kit (Qiagen #74136) and converted to cDNA using High-Capacity RNA-to-cDNA kit (Thermo #4387406 ...
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bioRxiv - Microbiology 2021Quote: ... 40 μl of media from each of 6 plant wells or media from 3 minimal media wells were pooled and stabilized in RNAprotect® Bacteria Reagent (QIAGEN) before performing RNA extraction using RNeasy Mini Kit (QIAGEN) ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA from cells in a single well of a 6-well plate was isolated using the RNeasy Plus Mini Kit (Qiagen, 74134) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA was extracted from cells (1-2 wells, 6 well plate) using an RNeasy Plus Mini Kit (Qiagen, Hilden, Germany), including a DNase step to remove residual DNA ...
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bioRxiv - Cell Biology 2021Quote: Cells were grown to 80% confluence in a 6-well plate before RNA was extracted using standard RNA extraction protocol (RNAeasy Mini Kit, Qiagen, #74104). cDNA synthesis was performed by mixing 1μg of RNA with qScript® cDNA Synthesis Kit (Quantabio ...
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bioRxiv - Genetics 2021Quote: ... S2 cells were seeded at 1 × 106 cells/ml in a 6-well plate and transfected using Effectene (Qiagen, Germantown, MD). For mobility shift assay (Figure 4) ...
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bioRxiv - Genetics 2022Quote: ... and non-inoculated G305-3M leaf segments collected along 10 different time points (0, 3, 6, 9, 12, 16, 24, 36, 48, 72 hpi) using the RNeasy Plant Mini Kit (Qiagen, Germany). The cDNA was synthesized from total RNA using a qScript™ cDNA Synthesis Kit (Quantabio ...
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bioRxiv - Physiology 2022Quote: ... liver and eWAT samples (n=6 per group) were extracted using RNABle lysis reagent (Eurobio) and the RNeasy® Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: Total RNA from hippocampus samples (n=6 per group) was extracted using RNABle lysis reagent (Eurobio) and the RNeasy® Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: DNA was extracted from 1×10^6 CD4 Th cells/ replicate using the Qiagen DNA mini kit according to manufacturer’s instructions (Qiagen Hilden, Germany). DNA was extracted from 10ng of mouse brain tissue as previously described13 using NEB Monarch High molecular weight DNA extraction kit according to manufacturer’s instructions (NEB Cat #T3050).
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bioRxiv - Microbiology 2023Quote: DNA was extracted from GWSS and control samples (nGWSS = 79, ncontrol = 6) using a DNeasy PowerSoil DNA Isolation kit (Qiagen, Germany) with minor changes to the manufacturer’s protocol as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... BON1/TGL/pQ and BON1/TGL/HA-Bcl-xL) grown on 6-cm dishes were used to isolate mRNA using RNeasy Plus Mini kit (Qiagen, 74136) containing gDNA Eliminator spin columns ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was isolated from cell pellets in batches of 6 using an RNeasy Plus Mini Kit (Cat # 74134, Qiagen, Hilden, Germany). Acceptable RNA concentration and quality were verified with Nanodrop and Bioanalyzer measurements ...
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bioRxiv - Bioengineering 2023Quote: ... DNA was extracted in pools of 6-8 adult mosquitoes mosquitoes using Blood & Cell Culture DNA Midi Kit (Qiagen, Cat# 13343) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... in vitro derived Foxp3+ cells were harvested on day 2 and day 6 into Trizol and total RNA was isolated with miRNeasy Micro Kits (QIAGEN 217084) according to the manufacturer’s instructions ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... RNA and protein were extracted 6 days post-transduction using the AllPrep RNA/Protein kit (Cat: 80204, Qiagen, Germantown, MD USA). RNA was converted to cDNA using SuperScript IV cDNA Synthesis Kit (Cat ...
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bioRxiv - Neuroscience 2024Quote: ... S2 cells were seeded at 1 × 106 cells/ml in a 6-well plate and transfected using Effectene (Qiagen, Germantown, MD). For coimmunoprecipitation (coIP ...
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bioRxiv - Microbiology 2024Quote: Microbial DNA was extracted from fecal samples of C57BL/6 mice using the QIAamp DNA Fecal Microkit (Stras Qiagen GMBH, Germany). A NanoDrop 2000 spectrophotometer (Thermoelectric Science ...
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bioRxiv - Cell Biology 2024Quote: APOB expression determined by first isolating total RNA from Hep3B cells grown in 6-well plates using the RNeasy Mini Kit (Qiagen, #74104). Real-time qPCR was performed using the TaqManTM RNA-to-CtTM 1-Step Kit (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: RNA was extracted from iPSCs plated for 48 h on a Matrigel-coated 6 WP with the RNAeasy Micro Kit (Qiagen #74004) according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was isolated from ripe pepper pericarp of RJ107(6)A3 (Maquilan et al., 2020) using RNeasy plant mini kit (Qiagen, Germany). First-strand cDNA was synthesized using SuperScript®III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Immunology 2024Quote: Cells were seeded (3 x 105) in 6-well plates and lysed using RLT buffer for RNA isolation (RNeasy Mini kit, Qiagen, #74106). Each sample was performed in triplicate ...
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bioRxiv - Neuroscience 2024Quote: ... and microglia (treated for 6 hours) treated with or without 1 µM CDDO-Me with a RNeasy Mini Kit (Cat. number: 74106; QIAGEN, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Cells were allowed to reach 80% confluency in a 6-well plate before total RNA was extracted using RNeasy kit (QIAGEN #74106) as per manufacturer protocol ...
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bioRxiv - Plant Biology 2020Quote: ... thaliana leaves using the RNeasy® Plant Mini Kit (Qiagen, Valencia, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was extracted using an RNeasy mini kit (Qiagen Inc., Valencia, CA) and the reverse transcription reaction was conducted using a Goscript™ Reverse Transcription System kit (Promega ...
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bioRxiv - Immunology 2021Quote: ... Gene expression was quantified using PowerUp SYBR green master mix (Qiagen, Valencia, CA) via quantitative real-time (qRT)-PCR ...
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bioRxiv - Cell Biology 2020Quote: RNA was isolated according with the RNeasy mini kit (74106; Qiagen, Valencia, CA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Further purified with Qiagen RNeasy clean-up columns (QIAGEN Inc., Valencia, CA, USA) as previously described [24].
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bioRxiv - Immunology 2021Quote: ... Total RNA was isolated using the RNeasy Plus Micro Kit (Qiagen, Valencia, CA). RNA concentration was determined with the NanopDrop 1000 spectrophotometer (NanoDrop ...
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bioRxiv - Microbiology 2020Quote: ... using a 5mm stainless steel bead and a TissueLyserII (Qiagen, Valencia, CA, USA) (2×1min ...
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bioRxiv - Developmental Biology 2021Quote: ... Nup107WT and Nup107D364N using the RNeasy mini kit (Qiagen Valencia, CA, USA; #74104). Briefly ...
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bioRxiv - Evolutionary Biology 2021Quote: We used the DNeasy Blood and Tissue Kit (Qiagen Inc., Valencia, CA, USA) to conduct non-destructive DNA extractions by piercing the cuticle of each specimen ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and genomic DNA was extracted using Qiagen DNeasy Plant kits (Qiagen, Valencia CA). Extracted DNA was quality and quantity screened using a QIAxpert microfluidics electrophoresis device (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... and total RNA was extracted using the miRNeasy mini kit (Qiagen, Valencia, CA) per manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... total RNA was extracted using RNAeasy Mini Kit (Qiagen Sciences, Valencia, CA, USA) and RT-PCR assays were performed ...