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Citations for Qiagen :
551 - 600 of 1472 citations for Ataxin 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from 1 × 106 cells using the RNeasy Plus Mini Kit (Qiagen), and cDNA was synthesized with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... HIV-1 RNA in plasma was extracted using the QIAamp Viral RNA Mini Kit (Qiagen). To amplify the 3’ half viral genome ...
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bioRxiv - Microbiology 2024Quote: ... All samples were subjected to a 1-hour DNAse treatment (Qiagen RNase-free DNase kit), and final elution volume was 20 μl ...
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bioRxiv - Biochemistry 2024Quote: ... 30 min) and supernatant was loaded onto a 1 ml Ni-NTA Superflow column (Qiagen). Proteins were eluted by an increasing imidazole gradient according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... for 1 hour at room temperature followed by isolation with a minElute RNA Cleanup Kit (Qiagen). 5 μg DNase-treated RNA was used as input for ribosomal depletion using the Epicentre (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 µg of RNA was reverse-transcribed to cDNA using the Quantitect Reverse Transcription Kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Snap frozen tissue samples or cells were homogenized in 0.5-1 ml Qiazol (Qiagen, Hilden, Germany) using a Retsch Homogenizer (Retsch GmbH ...
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bioRxiv - Molecular Biology 2021Quote: RNA was harvested from cell pellets of 1 million cells using the RNeasy Mini kit (Qiagen). DNA was digested using the TURBO DNA-free Kit (Ambion ...
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bioRxiv - Genetics 2020Quote: ... Conditions for a 25 μl PCR reaction using the 1 × Taq PCR Master Mix kit (Qiagen) were ...
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bioRxiv - Genomics 2019Quote: ... All wells were then pooled and diluted 5:1 (∼24 mL) in Buffer PB (Qiagen #19066) with 1/20th volume of 3 M NaOAc pH 5.2 (∼1.2 mL) ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 μg of RNA was reverse-transcribed into cDNA using the RT Omniscript cDNA kit (Qiagen), and 20 ng of cDNA template was amplified on a LightCycler-480 (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were subsequently homogenized at RT for 1 minute at 30Hz (Tissue Lyser II, Qiagen). 20ul of each homogenate was removed for protein concentration measurements (Pierce BCA Protein Assay Kit ...
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bioRxiv - Immunology 2021Quote: ... HIV-1 RNA was extracted from plasma samples using QIAamp Viral RNA Mini Kit (Qiagen, #52904). Followed by first-strand cDNA synthesis using Superscript III reverse transcriptase (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... Richard Vierstra24,54, and phytochrome 1 from Agrobacterium fabrum strain C58 (AgP1, gene Atu1990) in pQE12 (Qiagen) was a kind gift from Prof ...
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bioRxiv - Biochemistry 2020Quote: ... The cytosolic supernatant was incubated and stirred with 1 ml Ni-NTA agarose (Qiagen, Hilden, Germany) at 4 °C for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... and Halt Protease inhibitor (Thermo-Scientific) (1:100) for 1min at 30Hz using the TissuelyserLT (Qiagen). Homogenates were transferred to chilled Eppendorf tubes (−20°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Signal was detected using mouse anti-His antibody from Qiagen (Cat No./ID: 34660 1:500). Anti-mouse HRP was used for colorimetric development (Cat No./ID-A8924 1:1000) ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1 μg of total RNA was reverse transcribed using the Quantitect Reverse Transcription Kit (Qiagen) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM PMSF and the resulting supernatant was incubated with nickel-nitrilotriacetic acid agarose (QIAGEN) overnight at 4 °C ...
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bioRxiv - Bioengineering 2021Quote: ... PCR 2 products were purified by 1% agarose gel using a QIAquick Gel Extraction Kit (Qiagen), eluting with 15 μL of Elution Buffer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Total RNA (1 µg) was isolated from liver tissues using QIAzol lysis reagent (Qiagen, Hilden, Germany) and purified with RNeasy mini kit (Qiagen ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was extracted using the RNeasy Plant Mini Kit with in-column Dnase 1 digestion (Qiagen). 200 ng of total RNA were reverse transcribed using 500 ng of oligo(dT)15 and the ImProm-II Reverse Transcription System following the manufacturer’s instructions (Promega) ...
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bioRxiv - Neuroscience 2021Quote: ... the purification column was treated with 40μl of DNAse (diluted 1/8 in RDD Buffer) (Qiagen), incubated 15min and centrifuged at 8000×G for 15sec ...
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Cancer causes dysfunctional insulin signaling and glucose transport in a muscle-type specific mannerbioRxiv - Physiology 2021Quote: ... Muscles were homogenized 1 min at 30 Hz using a TissueLyser II bead mill (Qiagen, USA) in 300 μl ice-cold homogenization buffer ...
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bioRxiv - Microbiology 2021Quote: Total RNA was prepared from 5×106-1×107 cells using the RNeasy MIDI Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Tissues were homogenized in a mixture of chloroform and methanol (2:1) using TissueLyser II (Qiagen) and dried in a Vacufuge (Eppendorf ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA synthesis was performed from 1 μg of total RNA using the Omniscript RT Kit (Qiagen). All the samples were analyzed in triplicate using GoTaq qPCR Master Mix (Promega ...
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bioRxiv - Microbiology 2019Quote: ... and cell-associated HSV-1 DNA was isolated with the QIAamp DNA Blood Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... ulcerans CU001 strain calibrated at 1 McFarland = 106 CFU was extracted with EZ (Qiagen, Hilden, Germany). Then ...
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bioRxiv - Microbiology 2019Quote: cDNA from 1 µg of RNA was synthesized using the QuantiTect Reverse Transcription Kit (Qiagen, Germany) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: 1 µg of the total RNA was reverse transcribed using the QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was synthesized from 1 μg of total RNA using QuantiTect® reverse transcription kit (Qiagen) in 20 μl reaction volume ...
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bioRxiv - Biophysics 2022Quote: ... were filled with ( 1 mL | 0.5 mL ) of bed Ni-NTA resin (Qiagen or Thermo Fisher), and equilibrated with three rinses of Wash buffer (at least 30 resin bed volumes ...
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bioRxiv - Genetics 2022Quote: ... Midi, 3-4 ml of blood) and Puregene (0.3-1 ml of blood, manual extraction) (Qiagen, Cat# 1057048 ...
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bioRxiv - Cell Biology 2022Quote: ... run on a 1 % agarose gel and gel extracted using QIAquick Gel Extraction Kit (#28704, Qiagen). tdTomato and neomycin were cloned out from H3.1-miCOUNT (Denoth-Lippuner et al. ...
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bioRxiv - Cell Biology 2022Quote: ... run on a 1 % agarose gel and gel extracted using QIAquick Gel Extraction Kit (#28704, Qiagen). The plasmid backbone ...
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bioRxiv - Bioengineering 2020Quote: ... Each well is then diluted with 1 to 4 v:v in RNAse free elution buffer (QIAgen) to a total volume of 8 µL ...
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bioRxiv - Immunology 2021Quote: DNA from 1-2 stool pellets was extracted using the QIAamp DNA Stool Mini Kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and 1 μg of linearized DNA was transfected into A549 cells using Effectene Transfection Reagent (Qiagen) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Cultures samples were then mixed 1:2 with RNA Protect Bacteria Reagent (QIAGEN, Germantown, Maryland, USA), vortexed immediately for 5 seconds and incubated for 5 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... pyrosequencing was conducted on a PyroMark Q96 MD instrument using CpG LINE-1 assay (973043, Qiagen). To monitor bisulfite conversion efficiency ...
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bioRxiv - Immunology 2022Quote: ... freshly sorted G1-ILC populations (1 × 103 cells) were lysed with Buffer RLT (Qiagen, Hilden, Germany) and purified with RNAClean XP (Beckman Coulter ...
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bioRxiv - Physiology 2022Quote: ... was extracted from adipose tissue (experiment 1) using the Dneasy Blood and Tissue Kit (Qiagen, # 69504). We amplified both the mouse genomic gene Glyceraldehyde 3-phosphate dehydrogenase (Gapdh ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was collected and tumbled with 1 mL of preequilibrated Ni(II)-NTA Superflow (Qiagen) resin for 1-1.5 h at 4 °C.
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bioRxiv - Immunology 2020Quote: ... The bacterial lysates were mixed for 1 h with 5 ml of Ni-NTA resin (Qiagen) that had been equilibrated with buffer A ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR products were purified on a 1% gel and extracted using QIAquick Gel Extraction Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Total RNAs were prepared in RLT RNA lysis buffer supplemented with 1:100 β-mercaptoethanol (Qiagen) at 5,000 cells/µl ...
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bioRxiv - Cancer Biology 2019Quote: ... and 1 mg of RNA was reverse transcribed to cDNA using the Omniscript RT Kit (Qiagen). qPCR was performed using the TaqMan assay system ...
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bioRxiv - Immunology 2020Quote: ... Disruption of microbial cells was enhanced using TissueLyser II (QIAGEN, 30 shakes/sec., 1 min., 2x). To lyse Gram-negative bacteria ...
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bioRxiv - Microbiology 2020Quote: ... 1 ug of RNA was reverse transcribed to cDNA with the QuantiTect reverse transcription kit (Qiagen) according to the manufacturer’s instructions ...