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Citations for Qiagen :
551 - 600 of 941 citations for 3 Thiophen 3 yl benzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: RNA samples were isolated from seedlings at each time point with 3 biological replicates using Qiagen Plant RNA extraction kit (Qiagen; catalog no. 74904). A total of 18 cDNA libraries were prepared following the standard BGISEQ-500 RNA sample preparation protocol and sequenced by the DNBseq platform (BGI ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted from peripheral blood leukocyte samples from visit 2 or 3 using the Gentra Puregene Blood Kit (Qiagen; Valencia, CA, USA) according to the manufacturer’s instructions (www.qiagen.com ...
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bioRxiv - Microbiology 2022Quote: Total mouse lung RNA was extracted at days 0 and 3 post-challenge using the RNeasy Mini Kit as described above (QIAGEN, Cat. No 74004) and purified with the QIAquick PCR Purification Kit (QIAGEN ...
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bioRxiv - Systems Biology 2022Quote: Total RNA was isolated from whole brain tissue from 3 biological replicates per strain for 45 strains of the HRDP using QIAzol (Qiagen, Valencia, CA, USA). The brain was split sagitally and half of the brain was used for RNA sequencing ...
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bioRxiv - Immunology 2023Quote: Whole livers were harvested at indicated timepoints and placed into a 7 ml homogenizer tube (Omni International Cat. No. 19-651) containing 3 mL sterile PBS and one 5 mm stainless steel bead (QIAGEN Cat. No. 69989). Spleens and single granulomas were harvested at indicated timepoints and placed into a 1 ml homogenizer tube (Fisher Brand Cat ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.5μl of diluted cDNA (1:3 - nuclease-free water) was used as template in 10μl real-time PCR reactions (Qiagen: ‘SYBR Green PCR kit’) to assay specific transcripts (BioRad ...
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bioRxiv - Physiology 2023Quote: ... Ganglia in 1 mL of TRIzol reagent were homogenized using a TissueLyzer II bead mill (one 5 mm stainless steel bead per tube, 30 s-1 frequency for 3 minutes; Qiagen Inc. Hilden, Germany). The homogenates were incubated with 200 µL chloroform and centrifuged for 15 minutes at 12000 x g to isolate the protein-containing organic phase ...
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bioRxiv - Cancer Biology 2023Quote: ... The resultant backbone without the Puromycin gene was purified using the QIAquick PCR & Gel Cleanup Kit (Qiagen 28506. A DNA block (Table 3) containing the Blasticidin resistance gene sequence was synthetised (Integrated DNA Technologies ...
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bioRxiv - Genomics 2024Quote: ... Then the total volume was spun down and plasmid pools were extracted from the bacterial pellets using 2-3 columns of the Hi-Speed Plasmid Maxiprep kit (Qiagen, catalog no. 12663) per sublibrary ...
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bioRxiv - Genetics 2024Quote: ... comprising 1x reaction buffer QIAGEN Multiplex PCR Master Mix (containing dNTPs, HotStartTaq DNA polymerase and 3 mM MgCl2 as final concentration; QIAGEN, Venlo, the Netherlands), 0.05 µM tailed forward primer (see Suppl ...
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bioRxiv - Neuroscience 2024Quote: ... Locked nucleic acid (LNA) probes (Qiagen) to detect either sense (5’ TYE563-labelled CCCCGGCCCCGGCCCC ...
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bioRxiv - Immunology 2024Quote: ... RNA extraction was performed by QIAGEN Nucleic Acid Isolation Service (QIAGEN, Germany). RNA integrity number (RIN ...
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bioRxiv - Cell Biology 2020Quote: ... Circulating cell-free nucleic acid (ccfNA) was extracted using the Qiamp Circulating Nucleic Acid kit (Qiagen, USA) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... Approximately 150 mg FW of frozen plant material was disrupted with a glass and a metal bead (Ø 3 mm; QIAGEN GmbH, Hilden, Germany) in a TissueLyser (MM400 ...
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bioRxiv - Plant Biology 2022Quote: RNA samples were isolated from seedlings at each time point with 3 biological replicates using Qiagen Plant RNA extraction kit (Qiagen, Germany; Cat. No. 74904). A total of 18 cDNA libraries were prepared following the standard BGISEQ-500 RNA sample preparation protocol and sequenced by the DNBseq platform (BGI ...
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bioRxiv - Neuroscience 2023Quote: ... Dissociated microvascular cells were stained for flow cytometric analysis or were additionally processed with myelin removal beads (Miltenyi 130-069-731) and 3 sequential positive selections with CD31 microbeads (Miltenyi 130-097-418) for RNA isolation (Qiagen RNeasy Micro Kit 74004). cDNA library preparation used Oligo-dT at 60 million clusters/sample (University of Chicago Genomics Facility).
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bioRxiv - Immunology 2021Quote: ... Ni-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen) was used to purify the protein via gravity flow and proteins were eluted as previously described37,38 ...
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bioRxiv - Microbiology 2021Quote: ... and associated nucleic acid extraction (Qiagen, Germany) kits were used ...
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bioRxiv - Genomics 2024Quote: ... and QIAamp Circulating Nucleic Acid Kit (Qiagen) for EAC samples ...
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bioRxiv - Microbiology 2024Quote: ... Ni-nitrilotriacetic acid (NTA) beads (Qiagen, USA) were used to purify the protein ...
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bioRxiv - Pathology 2024Quote: ... the Circulating Nucleic Acid Kit (Qiagen, 55114) was employed with specific modifications to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... snap frozen samples were homogenized in an Eppendorf tube using a 3-mm Tungsten carbide beads and TissueLyser II system (Qiagen; 4 min at 30 Hz). RNA isolation steps were followed as mentioned above in this section ...
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bioRxiv - Microbiology 2024Quote: ... Biomass was immediately stabilized upon sampling by mixing 2 ml biomass with 6 ml PowerProtect DNA/RNA solution (1:3) (Qiagen Benelux B.V., Venlo, The Netherlands). The stabilized mixture was spun down and the remaining pellet was freeze-dried overnight and stored at −70°C ...
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bioRxiv - Genetics 2020Quote: Iron nitrilotriacetic acid (NTA) resin were prepared in-house by stripping metal ions from nickel nitrilotriacetic acid agarose resin (Qiagen) with 100 mM ethylenediaminetetraacetic (EDTA ...
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bioRxiv - Genomics 2021Quote: ... Total Nucleic Acid (TNA) was extracted using automated nucleic acid extraction on the QIAsymphony and the DSP Virus/Pathogen Mini Kit (Qiagen). RNA isolation and library preparation is fully described in Butler ...
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bioRxiv - Cancer Biology 2022Quote: ... using the QIAamp Circulating Nucleic Acid kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by a nickel-nitrilotriacetic acid column (Qiagen) and then dialyzed against buffer comprised of 10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... using the QIAamp Circulating Nucleic Acid kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Tris- ethylenediaminetetraacetic acid buffer was obtained from Qiagen® (Hilden ...
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bioRxiv - Cell Biology 2024Quote: ... or incubated with nickel-nitrilotriacetic acid resin (Qiagen). After washing with washing buffer the fusion constructs were eluted with elution buffer (20 mM HEPES ...
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bioRxiv - Plant Biology 2022Quote: ... 5’ and 3’ untranslated regions and open reading frames) for the paralogs were made using the CLC Main workbench 7 (QIAGEN® Aarhus A/S, Aarhus C, Denmark). Sequence alignments were generated for the genomic DNA ...
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bioRxiv - Neuroscience 2022Quote: ... Mice received either miRCURY LNA power inhibitor for mmu-miR-335-5p (Ant-335; sequence: 5’-CATTTTTCGTTATTGCTCTTG-3’; Qiagen, Cat No.: 339132; 0.1 nmol in 2 μL PBS), or a non-targeting scrambled control (Scr ...
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bioRxiv - Cancer Biology 2024Quote: ... was achieved from frozen tissue biopsies upon homogenization with stainless steel beads (3–7 mm mean diameter) using TissueLyser II (QIAGEN; 20” at 30 Hz, for 2 cycles(20)) ...
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bioRxiv - Biochemistry 2020Quote: ... Locked nucleic acid (LNA) ASOs were ordered from Qiagen and dissolved in water to 100 μM ...
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bioRxiv - Genomics 2021Quote: ... Using the QIAmp Circulating Nucleic Acid Kit (Qiagen, 55114) we followed two of the manufacturer protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Nucleic acids were extracted using the RNeasy kit (QIAGEN). Contaminating DNA was removed using the Turbo DNA-free kit (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: The antisense locked nucleic acid (LNA) GapmeR (#339515, Qiagen) sequence for the Tug1 LNA was (5’-3’ ...
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bioRxiv - Microbiology 2023Quote: ... nucleic acids were extracted (QIAmp DNA Mini Kit, QIAGEN) and a multiplex nested PCR was implemented to detect known skin-tropic HPyVs (MCPyV ...
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bioRxiv - Genetics 2023Quote: ... nucleic acid was purified using QiaQuick (QIAGEN, Germantown, Maryland) and sent to Laragen for Sanger sequencing (Laragen ...
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bioRxiv - Genomics 2020Quote: ... or the QIAmp Circulating Nucleic Acid Kit (Qiagen, Hilden, Germany), as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: A PowerSoil Deoxyribonucleic Acid (DNA) Extraction Kit (QIAGEN, Hilden, Germany) was used to extract genomic DNA from fecal samples according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... coli cell lysate using Ni-nitriloacetic acid agarose resin (Qiagen) under denaturing conditions according to the manufacturer’s guidelines ...
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bioRxiv - Biophysics 2020Quote: ... clarified lysate was applied to Ni-NTA (nitrilotriacetic acid, Qiagen) resin for 1.5h ...
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bioRxiv - Genomics 2020Quote: cfDNA was extracted using QIAamp Circulating Nucleic Acid Kit (Qiagen) with the Qiavac24s system ...
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bioRxiv - Microbiology 2022Quote: ... and an automated nucleic acid purification system (QIAcube Connect; Qiagen). Each eluent was reverse-transcribed ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated with nickel-nitrilotriacetic acid beads (Qiagen) for 2 h at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... and affinity-purified using Ni-nitrilotriacetic acid (NTA) agarose (QIAGEN) as previously described (Kim et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... cfDNA was extract by QIAmp Circulating Nucleic acid Kit (Qiagen) and quantified by Qubit (Qubit dsDNA High Sensitivity Kit ...
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bioRxiv - Biochemistry 2024Quote: ... Columns containing nickel-nitrilotriacetic acid (Ni-NTA) agarose (QIAGEN #30250) were equilibrated with 2 column volumes (CV ...
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bioRxiv - Genetics 2024Quote: ... Soluble proteins were incubated with nickel-nitriloacetic acid beads (Qiagen) for 2 h at 4°C ...