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Citations for Qiagen :
551 - 600 of 2997 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... RNA was harvested at 6 and 24 h post IFN-treatment (three independent biological triplicates) and isolated using the RNeasy Mini Kit (Qiagen). RNA-Seq was performed by Novogene using a PE150 sequencing strategy on the Illumina NovaSeq 6000 platform ...
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bioRxiv - Biochemistry 2022Quote: ... complexes were either concentrated using a 50 kDa MWCO concentrator and further purified on a Superose 6 Increase 10/300 column (Cytiva) equilibrated in low-salt CSFxB or loaded directly onto Ni2+ agarose (Qiagen), eluted using 300 mM imidazole ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells from single wells of a 6-well plate were pelleted and total RNA was extracted using the RNeasy MiniKit (QIAGEN), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Swabs were incubated at 37 degrees Celsius for 30 minutes then bead beaten for 6 minutes at 30Hz in a TissueLyser II (Qiagen). The rest of the protocol was followed increasing the volumes to account for the additional lysis buffer ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from FACS-sorted L-GMPs from BL/6 and Il21-/- AML mice using the RNeasy Micro Kit (Qiagen). RNA purity was checked using the NanoPhotometer® spectrophotometer (Implen) ...
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bioRxiv - Evolutionary Biology 2023Quote: We extracted total RNA from liver and BAT from each sample (n = ∼6 per genotype/sex/treatment/tissue) using the RNeasy PowerLyzer Kit (QIAGEN). We generated Illumina cDNA libraries from 1 μg of purified RNA using KAPA Stranded mRNA-Seq Kit (Illumina) ...
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bioRxiv - Developmental Biology 2023Quote: ... we used 6 mL of 55 °C Cell Lysis Solution from The Gentra Puregene® Cell and Tissue Kit (Qiagen) with 0.1 mg/mL proteinase K and 1% β-mercaptoethanol ...
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bioRxiv - Cell Biology 2023Quote: The entire RNA was isolated from a single well of a 6-well plate using the RNeasy Plus Micro Kit (QIAGEN), following the guidelines provided by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: Monolayer of 1.0 × 106 cells grown in 6 well plate was lysed in 500 μl/well QIAzol Lysis Reagent (Qiagen #79306) and RNA extraction was performed with QIAzol miRNeasy Mini Kit (Qiagen #217004 ...
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bioRxiv - Cancer Biology 2024Quote: RNA was extracted from cell lines grown in 6-well plates or from cell pellets using the RNAeasy Mini Kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: BMDM treated in 6-well plates were washed twice with PBS before total RNA purification using the RNeasy kit (Qiagen) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: DNA extraction from cultured treponemes propagated in 6-well plates following transformation and expansion was performed using the QIAamp DNA mini kit (Qiagen) according to the manufacturer’s instructions ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... at 6 m/s for 50 sec and total RNA extracted with the RNeasy Lipid Tissue Mini Kit (#74804; Qiagen). For SCP (D18 ...
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bioRxiv - Cell Biology 2024Quote: ... Azure Cielo 6 (Azure Biosystem) was used to detect the signal of mRNA expression level with QuiantiNova SYBR Green (Qiagen). Target genes included ZEB1 and FN1 for mesenchymal epithelial transition markers and TEAD1 and CTGF for Yap-associated markers ...
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bioRxiv - Cancer Biology 2024Quote: Cells were grown in 6-well culture dishes to 70-80% confluency and RNA was isolated with RNeasy Plus kit (#74034, Qiagen). CDNA was generated using High Capacity cDNA Reverse Transcription Kit (#4368814 ...
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bioRxiv - Neuroscience 2024Quote: ... was extracted and purified from pools of 30 zebrafish larvae at 6 dpf using the RNeasy Plus Mini Kit (Qiagen, Germantown ...
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bioRxiv - Genomics 2024Quote: ... Each sample was mixed with 5 µL of one of 6 Illumina adapter sequences (specified in the Qiagen protocol appendix) and 2 µL of ultralow input ligase ...
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bioRxiv - Neuroscience 2024Quote: ... in vitro hTAM were incubated for 6 hours at 37°C with predesigned siRNA against human SPP1 (Qiagen, cat.#1027416) or Scramble (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... After 6 h RNA was extracted and1 µg of total RNA was used for reverse transcription (Quantitect reverse transcription kit, Qiagen) as per instructions ...
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bioRxiv - Microbiology 2024Quote: ... 6×105 cells were seeded onto type I collagen-coated 6-well plates and transfected with 200 ng of viral DNA using Attractene Transfection Reagent (Qiagen). Two days later ...
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bioRxiv - Physiology 2024Quote: ... from TKOc and CTRL mice and livers of 3-month-old young and 18-month-old aged C57BL6/JN mice (n=6 per group, equally distributed males and females, total n=96 libraries) using a QIACube (Qiagen). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was isolated from mouse prostate cell lines or WT prostate tissue from 8-10 week old C57BL/6 mice using the RNeasy Mini Kit (Qiagen). Complementary DNA (cDNA ...
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysate was mixed with Ni-NTA agarose at 4℃ for 2h (Qiagen). Bound protein was eluted in 20 mM Tris pH 8.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... Lysates were treated with 4 μl of 100 mg/ml RNase A (QIAGEN) at 37 °C shaking at 500 RPM for 3 hours ...
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bioRxiv - Immunology 2021Quote: ... and kept at 4°C RNA was purified using RNEASY Mini Kit (Qiagen) and its concentration determined using a Nanodrop 1000 ...
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bioRxiv - Neuroscience 2021Quote: ... 4°C for 10 min and the pellet was lysed with RLT+ (Qiagen). For scRNA-sequencing ...
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bioRxiv - Microbiology 2021Quote: ... at 4°C until RNA was extracted using the RNeasy Mini Kit (Qiagen)
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bioRxiv - Microbiology 2020Quote: The MoBio PowerSoil-htp 96 kit (now Qiagen Cat No./Id: 12955-4), with minor modifications ...
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bioRxiv - Cancer Biology 2020Quote: ... cell pellets were lysed in a 4% SDS buffer using a Qiashredder (Qiagen). Relative densitometry for western blots was determined using ImageJ software and normalized to the density of loading control β-ACTIN ...
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bioRxiv - Microbiology 2021Quote: ... into a volume of 4 μL PBS sc 1X (Qiagen, Cat. No. 150345). To avoid evaporation of the PBS sc 1X during the sort ...
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bioRxiv - Genomics 2021Quote: ... 4 μL of room temperature Stop solution (REPLI-g Single Cell Kit, Qiagen) was then added and the samples were vortexed and spun down ...
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bioRxiv - Molecular Biology 2023Quote: ... Effectene (4 μL of enhancer and 5 μL of Effectene reagent; Qiagen 301427) was used to transfect 500 ng of the indicated eGFP plasmid ...
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bioRxiv - Synthetic Biology 2022Quote: ... 18 µl of Omniscript enzyme at 4 U/µl (Omniscript RT Kit, Qiagen), and 37 µl water ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was incubated overnight at 4 °C with Strep-Tactin Beads (Qiagen), typically using 0.75 ml packed beads per two liters of original culture volume ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with dsHsc70-4 with the Effectene Transfection reagent (301427, QIAGEN) and incubated for 72 h ...
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bioRxiv - Microbiology 2023Quote: ... supernatant was incubated overnight at 4°C with Ni-NTA agarose beads (Qiagen) used to purify the recombinant proteins as described [40] ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Crystals were grown at 4 °C in EasyXtal-15-Well Tools plates (Qiagen), using hanging-drop vapour diffusion ...
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bioRxiv - Neuroscience 2023Quote: Cultured cells were lysed at 4°C in RLT buffer (Qiagen, Courtabœuf, France) and stored at -80°C ...
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bioRxiv - Microbiology 2024Quote: ... DNA was isolated with the DNeasy UltraClean 96 Microbial Kit (Qiagen 10196-4). Library preparation and sequencing were performed at the NGS Competence Center NCCT (Tübingen ...
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bioRxiv - Genomics 2024Quote: ... with 4 µl of RNase A (100 mg/ml) (Qiagen, Germantown, MD, USA), The extraction procedure was then followed by the manufacturer’s manual ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were harvested by scraping in 4 mL lysis buffer (RLT buffer (Qiagen) + 143 mM β-ME) ...
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bioRxiv - Biochemistry 2024Quote: ... The soluble fraction was then added to 4 mL Ni-NTA beads (Qiagen) pre-washed in water ...
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bioRxiv - Genetics 2024Quote: ... Samples were cooled and 4 µL 100 mg/mL RNAse A (Qiagen, #19101) was added ...
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bioRxiv - Genomics 2021Quote: ... and Vero E6 cells infected with 2 patient virus isolates was converted to cDNA using reverse transcriptase from qiaseq SARS-CoV-2 primer pool (Qiagen kit, cat no. 333896). APOBEC3b (sense ...
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bioRxiv - Developmental Biology 2021Quote: ... for 2 min at 40 Hz using TissueLyser LT (Qiagen). 500 µl PBS-tween (0.01% ...
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bioRxiv - Microbiology 2021Quote: ... 2 mL of nickel-charged resin (Qiagen, Ni-NTA Agarose) was added to a column (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... using the QIAstat-Dx Respiratory SARS-CoV-2 Panel (Qiagen). Viral stocks were produced infecting at a multiplicity of infection (MOI ...
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bioRxiv - Microbiology 2022Quote: ... in a 2 mL cryovial using a tissue lyser (Qiagen) and 0.2mm ceramic beads ...
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bioRxiv - Microbiology 2021Quote: ... or using the QIAseq SARS-CoV-2 Primer Panel (Qiagen) (for organoid supernatants) ...
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bioRxiv - Genomics 2021Quote: ... QIAseq SARS-CoV-2 Primer Panel (Qiagen, cat. no. 333895) and QIAseq FX DNA Library Kit were used in order to prepare amplicon libraries for viral genome sequencing ...