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Citations for Qiagen :
501 - 550 of 1566 citations for Rat TRPV1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and pUC18 plasmids were isolated by miniprep (QIAGEN) from these colonies for sequencing.
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bioRxiv - Biochemistry 2022Quote: ... and colonies were selected for plasmid Maxiprep (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmid DNA purification kit were purchased from QIAGEN Inc (Valencia ...
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bioRxiv - Microbiology 2022Quote: ... and the plasmids were isolated by miniprep (Qiagen) and sequenced individually using T3 and T7-Pro primers ...
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bioRxiv - Neuroscience 2022Quote: ... or the EndoFree Plasmid Maxi Kit (Qiagen, 12362). For in utero electroporations ...
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bioRxiv - Immunology 2023Quote: ... or the QIAGEN® Plasmid Mini Kit (QIAGEN). For the extraction using the QIAGEN® Plasmid mini Kit ...
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bioRxiv - Genetics 2023Quote: ... or the Qiagen Plasmid Midiprep Kit (12143; Qiagen), and sequence verified using the Sanger method at the Johns Hopkins School of Medicine Synthesis & Sequencing Facility.
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bioRxiv - Genetics 2023Quote: ... then purified using EndoFree Plasmid Maxi Kit (Qiagen). The gRNA spacer sequences for each of the deletions are listed in Supplementary table 2.
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bioRxiv - Developmental Biology 2023Quote: ... Plasmid DNA was extracted by miniprep (Qiagen, 27104). All plasmid verification was performed by Sanger sequencing (Azenta ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the plasmid library extracted via miniprep (Qiagen). For large-scale libraries level 3 barcode pools were generated in order to ensure a unique barcode-to-circuit mapping ...
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bioRxiv - Systems Biology 2022Quote: ... Plasmid DNA prepared by maxi-prep (Qiagen, 12162) were transfected into adherent HEK293T cells using Lipofectamine 3000 (Thermo-Fisher ...
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bioRxiv - Immunology 2022Quote: ... Plasmid DNA was purified (Qiagen HiSpeed Maxiprep Kit) precipitated with ethanol ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmids were purified using Qiagen miniprep kits (Qiagen) and sequence verified by Sanger sequencing (Genewiz) ...
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bioRxiv - Microbiology 2023Quote: ... or Plasmid Plus Maxi Kits (QIAGEN, Cat. #12963) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... The plasmid extraction kit was purchased from Qiagen GmbH (Hilden ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids from transformants were isolated by miniprep (Qiagen), screened for the correct plasmid by colony PCR or plasmid digest ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmids were harvested by Midi-prep (12243, Qiagen). To produce lentivirus ...
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bioRxiv - Biochemistry 2023Quote: ... coli and plasmids were recovered by miniprep (Qiagen). Plasmids were checked by Sanger sequencing (Genewiz).
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bioRxiv - Genomics 2024Quote: ... and the plasmid was purified by miniprep (Qiagen). Purified plasmids were screened for the shRNA oligo insert using XhoI digestion and sequenced for validation.
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bioRxiv - Biophysics 2024Quote: ... or a Qiagen Plasmid Midi Kit (Qiagen, #12145). Correct insertions were verified by Sanger sequencing (Lightrunc ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... The plasmid library was extracted using Midiprep (Qiagen). In parallel ...
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bioRxiv - Microbiology 2024Quote: Plasmid pDC-sgRNA was purified (Qiagen miniprep kit) and verified by nanopore sequencing (Plasmidsaurus) ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA was isolated from rat blood cells using QIAmp DNA Mini Kit (Qiagen, City, state) following manufacturer’s instructions using a QIAcube automated device ...
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bioRxiv - Microbiology 2021Quote: ... Parasite cultures were synchronized by repeated sorbitol treatment and 100 μg of purified plasmid DNA (Plasmid Midi Kit, Qiagen, Valencia, CA) was transfected in P ...
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bioRxiv - Cancer Biology 2019Quote: ... Bacterial cells were pelleted by centrifugation at 6,000 RCF at 4°C for 15 minutes and plasmid DNA was extracted using a Qiagen Plasmid Plus Midi Kit (Qiagen #12943). To estimate the total number of unique barcodes present in the plasmid pool ...
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bioRxiv - Plant Biology 2019Quote: ... 50,000 protoplasts in a volume of 100 μL were transformed with 20 μg of each plasmid (2 μg/μL) purified using the Plasmid Maxi kit (Qiagen, Germany). One batch of protoplasts was treated with an equivalent amount of water and used as the negative (untransformed ...
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bioRxiv - Physiology 2021Quote: ... Crtc2 and GFP expression plasmids were purified from DH5a Escherichia coli cultures using an EndoFree plasmid kit (Qiagen, Valencia, CA, USA), and resuspended in 71 mM sterile PBS ...
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bioRxiv - Microbiology 2021Quote: Plasmid DNA isolation was performed on individual sewage pellets (420 mg) using Plasmid Purification Mini Kit (Qiagen, Cat No./ID: 12123) with QIAGEN-tip 100 (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... The bacterial colonies were cultured with Zeocin selection at large scale and maxi-plasmid preparations were carried out using HiSpeed Plasmid Maxi Kit (Qiagen, 12662). CpG Methyltransferase ...
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bioRxiv - Cancer Biology 2022Quote: ... we cultured 250 ml Escherichia coli bacteria bearing transformed plasmids and isolated the plasmids using Qiagen Maxi-Prep Endotoxin-free kit (Qiagen; 12362) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Positive plasmids were extracted from overnight liquid cultures obtained from replica colonies by using the Plasmid Mini kit (Qiagen, Germantown, MD), and two to five clones for each strain were sequenced to ensure sequence fidelity to the previously cloned templates (35) ...
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bioRxiv - Molecular Biology 2019Quote: ... The cloned plasmid was amplified in DH5-Alpha cells and purified with QIAfilter™ Plasmid Maxi Kit (Qiagen, catalogue number 12243). Plasmid concentration was measured using Qubit (Thermofisher ...
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bioRxiv - Synthetic Biology 2022Quote: ... After overnight growth colonies were scraped into a collection bottle and plasmid pools were extracted using a plasmid maxiprep kit (Qiagen #12662). 2 smaller plates of LB with carbenicillin were also prepared with 1:20 diluted cells to count colonies and confirm transformation efficiency ...
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bioRxiv - Biochemistry 2022Quote: ... pTripZ-M1 plasmids containing PRC1 subunit fusion gene and the puromycin selective marker as well as the pTripZ-G2-YFP-CBX2(ΔTRE) plasmid harboring a geneticin selective marker were purified according to the protocol listed in EndoFree Max Plasmid Kit (QIAGEN; 12362). 24-hours before transfection ...
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bioRxiv - Synthetic Biology 2023Quote: ... The recombinant plasmid was then purified at a high concentration (200 μg/mL) using the plasmid preparation kit (Qiagen, Germantown, MD).
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bioRxiv - Developmental Biology 2023Quote: ... The cells containing the desired plasmids were amplified by broth culture and the plasmids were prepared by maxiprep kit (Qiagen, 12663). The plasmids were stored at −20°C until use.
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bioRxiv - Immunology 2023Quote: ... A 1.5ml aliquot of Stbl2 cells from overnight culture was used for miniprep plasmid extraction (QIAGEN Plasmid mini kit, Cat. 12123), to check the EEV sequence ...
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bioRxiv - Synthetic Biology 2023Quote: The ASKA-clone encoding tyrS was isolated from AG1 carrying this plasmid using the Qiagen Plasmid Midi (Qiagen Inc., CA, USA) and transformed into BL21-AI ...
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bioRxiv - Cancer Biology 2023Quote: ... The DDR-CRISPR library plasmid DNA was purified from transformed bacterial culture using a Qiagen EndoFree plasmid DNA Maxi kit (Qiagen, #12362).
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bioRxiv - Microbiology 2021Quote: ... 4×106 HEK293T cells or 2×106 HeLa cells seeded in a 100 mm dish were transfected with 10μg of each plasmid purified using the endotoxin-free plasmid isolation kit (Qiagen, Germantown, MD, USA) with TransIT-293 (Mirus ...
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bioRxiv - Microbiology 2022Quote: ... A wild-type virus stock NL4-3 was prepared by transfection of the pNL4-3 plasmid (purified using the Qiagen Plasmid Maxikit) into HeLa cells ...
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bioRxiv - Microbiology 2021Quote: ... Isolates were cultured overnight on sheep blood agar and plasmid DNA was extracted using Qiagen Plasmid Midi kit (Qiagen Inc, Germantown, MD) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... coli cells were cultured at 37°C and plasmid isolation was carried out using a Qiagen Plasmid Mini Kit (Qiagen, Germantown, MD) according to manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... 293A cells were transfected with the plasmid of pCDNA3.1-ACE2 (human)-3×FLAG (MiaoLing Plasmid Platform, China) using polyfect (QIAGEN, West Sussex, UK) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmid transfections were achieved using Effectene transfection reagent (Qiagen) according to the manufacture’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... followed by plasmid miniprep using QIAprep Spin Miniprep (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... aliquots were pooled prior to plasmid purification (Qiagen, 12963). For each of the aliquots ...
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bioRxiv - Microbiology 2021Quote: ... using QIAGEN Plasmid Mini and Midi Kit (Qiagen, Germany) and subjected for Sanger sequencing (Eurofins ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid preparations (Qiagen mini prep kit Cat no.27106) were sequenced using plasmid-specific primers (Table S5) ...
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bioRxiv - Cell Biology 2022Quote: Plasmid transfection was implemented using the Effectene reagent (Qiagen) according to the manufactures’ protocols ...