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Citations for Qiagen :
501 - 550 of 10000+ citations for Mouse Fibroblast Growth Factor 15 FGF15 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... bacterial cells reaching an OD620nm = 0.6 were pelleted (5000 x g, 15 min) and immediately fixed in 1.5 ml RNA-protect (Qiagen) for 10 min before being processed using a PureLink RNA mini kit (Ambion ...
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bioRxiv - Developmental Biology 2023Quote: Approximately 15 female flies of CantonS were dissected in 1XPBS before being transferred to tubes containing RNAlater (Qiagen) on ice ...
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bioRxiv - Microbiology 2023Quote: ... RNA samples were treated on-column with DNAse for 15 min at room temperature (Cat. No. 79254, Qiagen). A total of 1µg of purified RNA was used to make cDNA by adding gDNA wipeout (2 μL) ...
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bioRxiv - Biochemistry 2023Quote: ... The mixture was incubated at 37 °C for 15 min and then quenched by adding RLT buffer (Qiagen). The RNA was then extracted using RNeasy kit (Qiagen) ...
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bioRxiv - Cell Biology 2024Quote: HEK293 cells were seeded in 15 cm plates and transfected after 24h according to manufacturer protocols (Effectene, Qiagen) using 5 µg of plasmid DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... the membrane was incubated with mouse primary antibody α-His (1:5000, Monoclonal mouse Tetra-His antibody, Qiagen, #34670) in 2.5% BSA in PBS-T overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... and low molecular weight DNA isolated for downstream analysis using a 1.2x volumes AMPureXP SPRI-beads and eluted in 15 μl EB buffer (Qiagen). ATAC-seq libraries were amplified using 5 μl each of the i5 and i7 Nextera Indexing primers and 25 μl of 2x HiFi HotStart ReadyMix (Roche Diagnostics ...
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bioRxiv - Developmental Biology 2021Quote: Pools of twenty 15 dpf-embryo (zmiz1a+/- and zmiz1a-/-) were used for total RNA isolation with RNAeasy columns (Qiagen) from five independent crosses ...
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bioRxiv - Neuroscience 2020Quote: ESR1 expression was manipulated with custom-designed locked nucleic acid (LNA™) 15-mer antisense oligonucleotides designed by Qiagen following Kelly & Goodson.41 The antisense oligo for ESR1 knockdown (ESR1-KD ...
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bioRxiv - Developmental Biology 2020Quote: ... using the QIAGEN TissueLyser at 15 Hz for 2-3 min with a Stainless-Steel Bead (QIAGEN catalog # 69989). Phase separation was induced with chloroform ...
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bioRxiv - Immunology 2020Quote: ... cells were first washed and pelleted five times with 15 ml of PBS then lysed with QIAzol reagent (Qiagen), RNA was extracted according to manufactures directions ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was cleared by centrifugation at 10,000g for 30 min at 4 °C and applied to 15-mL equilibrated Ni-NTA beads (Qiagen). The Ni-NTA column was washed with 150 mL of buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was cleared by centrifugation at 10,000g for 30 min at 4 °C and applied to 15-mL equilibrated Ni-NTA beads (Qiagen). The Ni-NTA column was washed with 150 mL of buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2023Quote: ... Plasmids were allowed to air dry in a fume hood for 15 minutes before resuspension in 500µL Buffer TE (Qiagen). Plasmid concentration was quantified using a Nanodrop 2000.
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bioRxiv - Evolutionary Biology 2024Quote: ... The PCR mix contained 0.16 µM of each of the fluorescent universal primer and of the reverse specified primer and 0.04 µM of the 5’ tail forward primer in a final 15 µl reaction volume (2x QIAGEN Multiplex PCR Master Mix with 3 mM Mg2+ ...
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bioRxiv - Bioengineering 2024Quote: ... spun down for 15 s at 8,000 g and eluted 3 times by adding 700 µL Buffer RW1 (Qiagen) and spinning ...
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bioRxiv - Microbiology 2020Quote: ... a monoclonal mouse Strep-tag antibodies (#34850, Qiagen), a monoclonal anti-actin antibody (A5441 ...
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bioRxiv - Cell Biology 2023Quote: ... or siRNA for Anxa2 (Mouse, Qiagen, Mm_Anxa2_3, SI00167496).
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bioRxiv - Biochemistry 2023Quote: Mouse EL4 genomic DNA was isolated by QIAGEN Blood & Cell Culture DNA Kit (QIAGEN ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse tissues were homogenized using TissueLyser II (Qiagen) and stainless-steel beads (5 mm ...
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bioRxiv - Microbiology 2020Quote: Oral fluid samples were prepared by homogenization of 200 μl sample (30 sec at 15 Hz) in a Tissuelyser II (Qiagen) followed by centrifugation (2 min at 10.000 rpm) ...
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bioRxiv - Cancer Biology 2021Quote: Protein lysate [1μg/μL] was RNase A digested at 37°C for 15 min following addition of 1 μg RNase A (Qiagen) per 25 μg protein ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The tissue was ground to a fine powder by adding 10-15 zirconia beads (1 mm diameter) and using a TissueLyser (Qiagen) for sunflower ligules ...
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bioRxiv - Immunology 2019Quote: ... 6µL ethanol/phosphate buffer (85:15 v/v ethanol/10mM phosphate buffer) were added and the tissue was homogenized (TissueLyser II, Qiagen). Samples were centrifuged at 5000g for 5 minutes at 4°C and the supernatants were transferred to a fresh tube and used for analyses ...
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bioRxiv - Cancer Biology 2019Quote: ... The lysate was then centrifuged at 15 000 ×g and the supernatant was added to the 1 mL Ni-NTA resin (Qiagen). After washing with Buffer A supplied with 30 mM imidazole ...
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bioRxiv - Physiology 2019Quote: ... 72 °C - 10 min) and amplicons separated using a Qiaxcel Advanced Separation System using 15 - 3000 bp markers (Qiagen, UK).
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bioRxiv - Genetics 2021Quote: Amplified libraries were then cleaned by pooling 25 uL per well into a 15 mL conical tube and cleaned via a Qiaquick PCR purification column following manufacturer’s protocol (Qiagen 28106). The pooled sample was eluted in 50 uL 10 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2020Quote: ... spin column and proper buffer washing steps were carried out following manufacturer recommendations including a 15 min on-column DNAse (Qiagen, ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Imidazole pH 8.0 was added to the detergent phase to a final concentration of 15 mM and the mixture was incubated with Ni-NTA agarose beads (Qiagen) for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 % (v/v) glycerol) for 30 s at a frequency of 15 s-1 using a TissueLyser II (QIAGEN). As the tissue homogenates were to be used for both native and SDS PAGE ...
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bioRxiv - Biophysics 2022Quote: ... The lysates were centrifuged (15 min, 27000 ×g) and the supernatants were applied to gravity flow columns packed with Ni-NTA Superflow (QIAGEN). The columns were washed with buffers (20 mM NaH2PO4 ...
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bioRxiv - Biophysics 2022Quote: ... before rapidly cooling the cell suspension on ice.22 The lysate was centrifuged (15 min, 27000 g) and the supernatant was applied to gravity flow columns packed with Ni-NTA Superflow (QIAGEN). The columns were washed with buffers (20 mM NaH2PO4 ...
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bioRxiv - Cell Biology 2022Quote: Pellets were thawed and lysed on ice in 15 mL lysis buffer containing freshly added lysozyme and benzonase prepared according to manufacturer instructions (Qiagen Qproteome Bacterial Protein Prep Kit ...
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bioRxiv - Microbiology 2022Quote: ... from the top A horizon were immediately collected for DNA extractions in 15 mL tubes containing 5 mL Lifeguard Soil Preservation Solution (QIAGEN) using chilled soil-processing trays ...
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bioRxiv - Genomics 2020Quote: ... The ethanol was then poured out and the pellet was air-dried for 15 minutes before resuspension in 50 to 100 μL of DNA Hydration Solution (Qiagen). DNA was sequenced with paired end Illumina sequencing at 30x coverage.
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bioRxiv - Cell Biology 2021Quote: ... The remaining lysate was transferred to a 15 ml falcon tube and 1 ml of a 50% slurry consisting of NiNTA agarose beads (Qiagen) in resuspension buffer was added per 10 ml of lysate ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were removed and the remaining cell pellet (500 μl) was transferred into a 15 ml tube containing 2.5 ml RNAprotect® Cell Reagent (Qiagen). The cells were resuspended by vortexing ...
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bioRxiv - Immunology 2020Quote: ... cleaning were used to purify 100 μl of pooled cDNA with final elution in 15 μl PCR-grade H2O (Qiagen). After quantification with Qubit dsDNA HS assay (Thermofisher) ...
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bioRxiv - Immunology 2021Quote: ... and subsequent crystallization optimization was performed manually using hanging-drop vapor diffusion method with 15-well screw-cap crystallization plates (Qiagen). The H5.28-HA head complex was crystallized in 7-9% PEG 8000 ...
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bioRxiv - Biochemistry 2022Quote: ... Imidazole pH 8.0 was added to the detergent phase to a final concentration of 15 mM and the mixture was incubated with Ni-NTA agarose beads (Qiagen) for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was purified using the RNeasy Minikit and DNase-treated at room temperature for 15 min using the RNase-free DNase set (Qiagen). cDNA was synthesized using the Maxima H Minus cDNA synthesis master mix (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... transferred to 2 ml lysis matrix B tubes (MPBio) and subjected to bead beating for 15 min at 30 Hz (Tissuelyser II, Qiagen) with RNAse A added ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was digested on-column for 15 min at room temperature using the RNase-free DNase I Set (Qiagen). First-strand complementary DNA (cDNA ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... 45 cycles at 95° for 15 sec and 60° for 1 min were performed in the Rotor-Gene Q real-time PCR cycler (Qiagen). The numbers of copies of the RdRP gene in the samples were calculated using an RdRP cDNA standard curve.
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bioRxiv - Biochemistry 2023Quote: ... Crystals were grown at 30°C by the hanging drop vapor diffusion method using 2 μL sample drops and 300 μL crystallization solution in a sealed chamber (EasyXtal 15-Well Tool, Qiagen). Crystals were soaked for 1h (for the Na structure or with the dibromo Intronistat B derivative ...
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bioRxiv - Molecular Biology 2023Quote: ... or to mature primate-specific miR-608 (AM608, 15-mer, as a control with no predicted complementary sequences in mice) (LNA, Exiqon, Qiagen). DIO mice were injected intravenously with 3.3 mg/kg oligonucleotide for three consecutive days and were sacrificed 0 ...
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bioRxiv - Neuroscience 2023Quote: ... according to the instructions and underwent 15-min on-column DNase digestion using an RNase-Free DNase Set (79254, Qiagen). cDNA was prepared with reverse transcriptase using an Omniscript RT kit (205113 ...
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bioRxiv - Microbiology 2023Quote: ... Triplicate PCRs were carried out in 15 µl reactions containing 1x UCP Multiplex PCR Master Mix (Qiagen, Venlo, The Netherlands), 0.3 μmol l−1 each of the forward and reverse primers ...
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bioRxiv - Molecular Biology 2023Quote: ... Ligated fragments were then amplified for 12-15 cycles using primers incorporating unique dual index tags with VeraSeq polymerase (Qiagen). Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases.
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bioRxiv - Cell Biology 2019Quote: ... the mouse anti-penta-His antibody (Qiagen; Catalog #34660) was used at a 1:2,000 dilution in 3% BSA in PBS with sodium azide ...