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Citations for Qiagen :
501 - 550 of 1798 citations for Human PLA2G4F shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and purified using a Plasmid Maxiprep kit (Qiagen).
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bioRxiv - Biochemistry 2020Quote: ... The plasmid DNA was isolated via miniprep (Qiagen) and sequenced prior to initiating the next mutation.
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid DNA was purified either as minipreps (Qiagen) or maxipreps (Thermo Fisher) ...
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bioRxiv - Microbiology 2019Quote: The preparation of plasmid DNA (Qiagen, Valencia, CA), restriction endonuclease digestion (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... or plasmids (QIAprep® Spin Miniprep Kit, Qiagen). Polymerase chain reaction (PCR ...
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bioRxiv - Microbiology 2021Quote: Plasmid DNA was isolated using miniprep kits (QIAGEN), with modifications for GBS as described elsewhere (32) ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection of plasmids was done with Polyfect (QIAGEN) or Polyethylenimine (PEI ...
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bioRxiv - Microbiology 2021Quote: Plasmid DNA was isolated using miniprep kits (QIAGEN), with modifications for GBS as described elsewhere (28) ...
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bioRxiv - Microbiology 2020Quote: ... and collected cells for plasmid DNA extraction (Qiagen). Next ...
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bioRxiv - Systems Biology 2021Quote: ... and used the EndoFree Plasmid Maxi Kit (Qiagen) to isolate plasmid according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmids were prepared by Maxiprep (Qiagen, Valencia, CA) and eluted in RNase-free water ...
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bioRxiv - Immunology 2021Quote: ... Plasmids were purified using MaxiPrep Kits (Qiagen, #12263) and mutations were verified by sequencing (Psomagen ...
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bioRxiv - Immunology 2021Quote: ... produced using the EndoFree Plasmid Mega Kit (Qiagen), or PBS for negative control groups ...
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bioRxiv - Genomics 2021Quote: ... plasmid DNA isolated (QIAprep Spin Miniprep Kit, Qiagen) and send for Sanger sequencing ...
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bioRxiv - Microbiology 2021Quote: ... and pUC18 plasmids were isolated by miniprep (QIAGEN) from these colonies for sequencing.
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bioRxiv - Biochemistry 2022Quote: ... and colonies were selected for plasmid Maxiprep (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmid DNA purification kit were purchased from QIAGEN Inc (Valencia ...
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bioRxiv - Microbiology 2022Quote: ... and the plasmids were isolated by miniprep (Qiagen) and sequenced individually using T3 and T7-Pro primers ...
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bioRxiv - Neuroscience 2022Quote: ... or the EndoFree Plasmid Maxi Kit (Qiagen, 12362). For in utero electroporations ...
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bioRxiv - Immunology 2023Quote: ... or the QIAGEN® Plasmid Mini Kit (QIAGEN). For the extraction using the QIAGEN® Plasmid mini Kit ...
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bioRxiv - Genetics 2023Quote: ... or the Qiagen Plasmid Midiprep Kit (12143; Qiagen), and sequence verified using the Sanger method at the Johns Hopkins School of Medicine Synthesis & Sequencing Facility.
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bioRxiv - Genetics 2023Quote: ... then purified using EndoFree Plasmid Maxi Kit (Qiagen). The gRNA spacer sequences for each of the deletions are listed in Supplementary table 2.
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bioRxiv - Developmental Biology 2023Quote: ... Plasmid DNA was extracted by miniprep (Qiagen, 27104). All plasmid verification was performed by Sanger sequencing (Azenta ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the plasmid library extracted via miniprep (Qiagen). For large-scale libraries level 3 barcode pools were generated in order to ensure a unique barcode-to-circuit mapping ...
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bioRxiv - Systems Biology 2022Quote: ... Plasmid DNA prepared by maxi-prep (Qiagen, 12162) were transfected into adherent HEK293T cells using Lipofectamine 3000 (Thermo-Fisher ...
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bioRxiv - Immunology 2022Quote: ... Plasmid DNA was purified (Qiagen HiSpeed Maxiprep Kit) precipitated with ethanol ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmids were purified using Qiagen miniprep kits (Qiagen) and sequence verified by Sanger sequencing (Genewiz) ...
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bioRxiv - Microbiology 2023Quote: ... or Plasmid Plus Maxi Kits (QIAGEN, Cat. #12963) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... The plasmid extraction kit was purchased from Qiagen GmbH (Hilden ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids from transformants were isolated by miniprep (Qiagen), screened for the correct plasmid by colony PCR or plasmid digest ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmids were harvested by Midi-prep (12243, Qiagen). To produce lentivirus ...
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bioRxiv - Biochemistry 2023Quote: ... coli and plasmids were recovered by miniprep (Qiagen). Plasmids were checked by Sanger sequencing (Genewiz).
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bioRxiv - Genomics 2024Quote: ... and the plasmid was purified by miniprep (Qiagen). Purified plasmids were screened for the shRNA oligo insert using XhoI digestion and sequenced for validation.
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bioRxiv - Biophysics 2024Quote: ... or a Qiagen Plasmid Midi Kit (Qiagen, #12145). Correct insertions were verified by Sanger sequencing (Lightrunc ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... The plasmid library was extracted using Midiprep (Qiagen). In parallel ...
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bioRxiv - Microbiology 2024Quote: Plasmid pDC-sgRNA was purified (Qiagen miniprep kit) and verified by nanopore sequencing (Plasmidsaurus) ...
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bioRxiv - Cell Biology 2021Quote: Oligonucleotides for siRNA included: human βPix (synthesized by Qiagen), human myosin-18A (synthesized by Qiagen)
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bioRxiv - Microbiology 2021Quote: ... Parasite cultures were synchronized by repeated sorbitol treatment and 100 μg of purified plasmid DNA (Plasmid Midi Kit, Qiagen, Valencia, CA) was transfected in P ...
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bioRxiv - Cancer Biology 2019Quote: ... Bacterial cells were pelleted by centrifugation at 6,000 RCF at 4°C for 15 minutes and plasmid DNA was extracted using a Qiagen Plasmid Plus Midi Kit (Qiagen #12943). To estimate the total number of unique barcodes present in the plasmid pool ...
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bioRxiv - Plant Biology 2019Quote: ... 50,000 protoplasts in a volume of 100 μL were transformed with 20 μg of each plasmid (2 μg/μL) purified using the Plasmid Maxi kit (Qiagen, Germany). One batch of protoplasts was treated with an equivalent amount of water and used as the negative (untransformed ...
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bioRxiv - Physiology 2021Quote: ... Crtc2 and GFP expression plasmids were purified from DH5a Escherichia coli cultures using an EndoFree plasmid kit (Qiagen, Valencia, CA, USA), and resuspended in 71 mM sterile PBS ...
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bioRxiv - Microbiology 2021Quote: Plasmid DNA isolation was performed on individual sewage pellets (420 mg) using Plasmid Purification Mini Kit (Qiagen, Cat No./ID: 12123) with QIAGEN-tip 100 (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... The bacterial colonies were cultured with Zeocin selection at large scale and maxi-plasmid preparations were carried out using HiSpeed Plasmid Maxi Kit (Qiagen, 12662). CpG Methyltransferase ...
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bioRxiv - Cancer Biology 2022Quote: ... we cultured 250 ml Escherichia coli bacteria bearing transformed plasmids and isolated the plasmids using Qiagen Maxi-Prep Endotoxin-free kit (Qiagen; 12362) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Positive plasmids were extracted from overnight liquid cultures obtained from replica colonies by using the Plasmid Mini kit (Qiagen, Germantown, MD), and two to five clones for each strain were sequenced to ensure sequence fidelity to the previously cloned templates (35) ...
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bioRxiv - Molecular Biology 2019Quote: ... The cloned plasmid was amplified in DH5-Alpha cells and purified with QIAfilter™ Plasmid Maxi Kit (Qiagen, catalogue number 12243). Plasmid concentration was measured using Qubit (Thermofisher ...
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bioRxiv - Synthetic Biology 2022Quote: ... After overnight growth colonies were scraped into a collection bottle and plasmid pools were extracted using a plasmid maxiprep kit (Qiagen #12662). 2 smaller plates of LB with carbenicillin were also prepared with 1:20 diluted cells to count colonies and confirm transformation efficiency ...
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bioRxiv - Biochemistry 2022Quote: ... pTripZ-M1 plasmids containing PRC1 subunit fusion gene and the puromycin selective marker as well as the pTripZ-G2-YFP-CBX2(ΔTRE) plasmid harboring a geneticin selective marker were purified according to the protocol listed in EndoFree Max Plasmid Kit (QIAGEN; 12362). 24-hours before transfection ...
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bioRxiv - Synthetic Biology 2023Quote: ... The recombinant plasmid was then purified at a high concentration (200 μg/mL) using the plasmid preparation kit (Qiagen, Germantown, MD).
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bioRxiv - Developmental Biology 2023Quote: ... The cells containing the desired plasmids were amplified by broth culture and the plasmids were prepared by maxiprep kit (Qiagen, 12663). The plasmids were stored at −20°C until use.