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Citations for Qiagen :
501 - 550 of 2365 citations for 1 Ethylbenz cd indol 2 1H ylidene malononitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... 2 or 4 days and genomic DNA was extracted using the DNeasy Blood and Tissue Kit (Qiagen). Bisulfite converted DNA libraries were prepared using the Accel-NGS Methyl-Seq DNA library kit (SWIFT BIOSCIENCES) ...
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bioRxiv - Genomics 2024Quote: RNA was extracted from 2-5 million flash frozen cells using the RNeasy plus mini kit (Qiagen). Libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New Englands Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was collected and incubated with 2 mL of Ni-NTA Superflow affinity resin (Qiagen), previously equilibrated with lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from pools of 2-3 organoids using RNeasy Plus Mini Kit (Qiagen, #74134) according to the manufacturer’s protocols ...
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bioRxiv - Synthetic Biology 2022Quote: ... first the filter-harvested cells were resuspended in 2 mL RNAprotect Bacterial Reagent (Qiagen Catalog no. 76506), then pelleted in a centrifuge ...
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bioRxiv - Immunology 2022Quote: ... the frozen lung specimens were weighed before homogenization in PBS/2% FBS solution using the TissueLyser (Qiagen), as previously described (Tseng et al. ...
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bioRxiv - Genomics 2022Quote: ... Samples were incubated overnight at 55°C before adding 2 µl of RNaseA (100 mg/ml, Qiagen) and incubated for 15 min at 45°C ...
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bioRxiv - Neuroscience 2023Quote: Total mRNA was extracted from the PBMC obtained from 2 alpacas using standard RNA extraction kit (Qiagen). Recovered mRNA was retrotranscribed to cDNA by using Supercript IV (ThermoFischer ...
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bioRxiv - Biochemistry 2022Quote: IQN17 was crystallized at room temperature in a hanging-drop vapor diffusion system by mixing 0.3 μL of 15 mg/mL peptide with 0.3 μL of reservoir solution containing 0.1 M imidazole (pH 8.0) and 35 % 2-methyl-2,4-pentanediol (MPD) (Qiagen). Crystals were seen two days after trays were set up ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted from 2 biological replicates of fecal pellets using DNeasy PowerLyzer PowerSoil kit (Qiagen). Concentration of Pc and Bt DNA was assessed using species-specific qPCR primers (Key Resources Table) ...
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bioRxiv - Genetics 2024Quote: ... the final pool was purified from a 2% agarose gel (QIAEX II Gel Extraction Kit, Qiagen #20021).
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bioRxiv - Immunology 2024Quote: mTEC subpopulations were sorted and lysed in 20µl Lysis Buffer (0.02% 2-ME in 2x TCL (Qiagen)) using BD FACSAria TM III cell sorter (BD) ...
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bioRxiv - Microbiology 2024Quote: ... then homogenised with a steel ball for 2 minutes at 25 Hz using a Tissue-Lyser (Qiagen). CFU was quantified by plating to MacConkey agar containing 50 ug / ml streptomycin.
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bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted at 2 or 8 hpi with DNeasy Blood/Tissue DNA mini kit (Qiagen) and quantitated by a nanodrop spectrophotometer ...
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bioRxiv - Plant Biology 2024Quote: ... Each extraction step was carried out for 2 h in a TissueLyser II (Qiagen AB, Sollentuna, Sweden) at 6 s-1 at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... and centrifuged (2 minutes 12,000 RPM) before DNA-containing supernatant (2µL) was added to PCR master mix (18µL, Qiagen 201203 ...
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bioRxiv - Bioengineering 2024Quote: ... lysis buffer and homogenized at 1000 rpm for 2 min with 5 mm Stainless Steel Beads (Qiagen) using 1600 MiniG Tissue Homogenizer (SPEX SamplePrep) ...
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bioRxiv - Bioengineering 2024Quote: ... plasmids were purified from the 2-mL culture and eluted using 50 µL EB buffer from QIAGEN. The concentration usually was 50 ng/µL ...
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bioRxiv - Cancer Biology 2024Quote: ... for 2 hours at 37°C and subsequently purified using the QIAquick PCR Purification Kit (Qiagen, 28106). The modified lentiGuide-puro vector (Addgene Plasmid #52963 ...
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bioRxiv - Cell Biology 2024Quote: ... with 2-mercaptoethanol and stored at -80 °C until purification with a RNeasy Plus Micro Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... each mock community (Supplementary Table 2) was extracted with EZ1 DNA Tissue Kit (Qiagen; 953034; EZ1 method) and a separate aliquot with TRI Reagent® (Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... the pCMVΔP1Δenv HIV-1 Gag-Pol packaging construct and the firefly luciferase-expressing HIV-1 vector at a 1:1:3 μg DNA ratio using Effectene transfection reagent (Qiagen). Recombinant luciferase-expressing viruses capable of a single round of replication were released into the cell medium and were harvested 48 h later ...
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bioRxiv - Microbiology 2023Quote: ... the pCMVΔP1Δenv HIV-1 Gag-Pol packaging construct and the firefly luciferase-expressing HIV-1 vector at a 1:1:3 µg DNA ratio using effectene transfection reagent (Qiagen). Recombinant ...
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bioRxiv - Microbiology 2023Quote: ... 5.0 x105 293T cells were co-transfected with HIV-1 Env-expressing and HIV-1 Tat-expressing plasmids at a ratio of 1:6 using Effectene (Qiagen) and incubated for 48 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... RNAs were isolated from 6-month H19ΔICR/H19ΔICR and H19+/H19+ littermates (2 per genotype) using RNeasy Plus Mini Kit (Qiagen). Samples with RNA Integrity numbers >9 were Ribosomal RNA depleted using RiboZero Gold Kit (Illumina) ...
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bioRxiv - Neuroscience 2020Quote: ... The 205bp amplicon from PCR 2 was gel extracted using the MinElute gel extraction kit (Qiagen Cat#: 28606), and sent to Genewiz for Amplicon-EZ targeted deep sequencing and Sanger sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... We pooled 2-3 fishes (6 weeks old) and extracted whole RNA using the RNAEasy Micro kit (Qiagen), with blood ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR #2 products were validated using gel electrophoresis and purified using the QIAquick Gel extraction kit (QIAGEN). The purified libraries were then quantified by Nanodrop and Qubit prior to sequencing on an Illumina MiSeq.
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bioRxiv - Systems Biology 2021Quote: ... Differences between samples and controls were calculated based on the 2−ΔΔCT method using RNU6 control primer (Qiagen) as normalizer ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was extracted from 2-week-old Arabidopsis seedlings using an RNeasy Plant Mini Kit (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... single cell directly into 2 µl lysis buffer (30 mM Tris-HCl [pH = 8.0], 10 mM NaCl, 0.2 µL Proteinase K [Qiagen, #19133] ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... The soluble fraction was split between two columns each with 2 ml Ni-NTA agarose beads (Qiagen, 30210) pre-equilibrated with Lysis Buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... The concentrated SPACA6-containing media was applied onto a 2-mL column of Ni-NTA agarose resin (Qiagen). The Ni-NTA resin was washed with 10 column volumes (CV ...
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bioRxiv - Immunology 2022Quote: ... The upper aqueous phase containing RNA was carefully transferred to a 2 ml collection tube (cat. 990381, Qiagen) without touching the interphase and placed in a QIAcube instrument for extraction.RNA extraction was carried out using the recommended protocol (FIW-50-001-J_FW_MB and PLC program version FIW-50-002-G_PLC_MB ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 RBD-His protein was purified from cell culture supernatant using a Ni-NTA (Qiagen) affinity column ...
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bioRxiv - Genomics 2020Quote: Total RNA of 2-3 million cells was isolated using miRNeasy Kit according to the manufacturer’s instructions (Qiagen). The quality of the RNA was assessed by a standard sensitivity NGS fragment analysis kit on Fragment Analyzer (Advanced Analytical Technologies) ...
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bioRxiv - Immunology 2021Quote: ... Both barcode and digested fragment products were run on a 2% gel and purified by gel extraction (Qiagen). NGS library was sequenced using an Illumina MiSeq and Illumina v3 MiSeq Reagent Kits with 150 base pair single-end sequencing according to standard manufacturer protocols ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR products were purified from 2% agarose gels via the QIAqick gel extraction kit (QIAGEN cat.#28704) and subjected to next-generation sequencing on a HiSeq instrument lane (Illumina ...
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bioRxiv - Physiology 2020Quote: Purified total RNA was extracted from fresh BM-MSCs at passage 2 using a Qiagen Minikit (Qiagen, Australia). Quantitative RT-PCR was performed (n=3 mice/group ...
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bioRxiv - Physiology 2020Quote: ... Groups of five flies were transferred to 2 mL tubes and were homogenized in 100μl PBS + 0.1% Triton X-100 using a TissueLyser LT (Qiagen). The samples were then spun down at 12,000 rpm and the supernatant transferred to fresh vials and the absorbance of each sample at 603 nm was measured using a NanoDrop ...
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bioRxiv - Genomics 2021Quote: Total RNA was collected from 2×106 CAR T cells with the RNEasy Plus Mini isolation kit (Qiagen). Library preparation and RNA-seq was performed by BGI America (Cambridge ...
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bioRxiv - Biochemistry 2021Quote: ... brucei genomic DNA was isolated from ∼ 2 × 108 bloodstream form cells using a DNeasy Blood & Tissue Kit (Qiagen) using standard methods.
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bioRxiv - Genomics 2021Quote: ... cfDNA was isolated from 2 ml of plasma using either the manual QIAamp circulating nucleic acid kit (Qiagen), or the semi-automated QIAsymphony DSP Circulating DNA Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... for the SARS-CoV-2 low stringency screen with the QIAmp DNA Blood maxi kit (Qiagen ref. 51194). In a first PCR step ...
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bioRxiv - Microbiology 2021Quote: Frozen plant tissue was ground using the TissueLyser II (QIAGEN; 2 cycles of 30 seconds at 27 Hz) and 3-mm zirconium oxide beads (Glen Mills Inc.) ...
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bioRxiv - Immunology 2021Quote: RNA of cells exposed to SARS-CoV-2 was isolated with the QIAamp Viral RNA Mini Kit (Qiagen) according to the manufacturers protocol ...
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bioRxiv - Microbiology 2023Quote: ... and homogenised with 3-5 mm steel beads in a TissueLyser II (Qiagen, 24 Hz, 2 × 3 min). 350 μL lysed blood or 200 μL lysed head kidney were transferred to a Magna Pure 96 Processing Cartridge (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... Mosquito samples were homogenized at 30 Hz for 2 minutes using a TissueLyser II (QIAGEN GmbH, Hilden, Germany) and centrifuged for 60 s at 11,000 rpm ...
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bioRxiv - Microbiology 2023Quote: ... 150 μL of the aggregated or non-aggregated cells were then washed with 2 volumes of RNAprotect (Qiagen) to prevent RNA degradation ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μl of diluted cDNA template was mixed with 4 μl QuantiTect SYBR Green PCR Master Mix (Qiagen), 100 nM forward primers (see Table 1 for sequences ...