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Citations for Qiagen :
451 - 500 of 581 citations for PhotoCol Methacrylated Type I Collagen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Brain and spinal cord tissue from Prp-TDP43A315T (Tg) animals and wild type (Wt) littermates were homogenized using a TissueRuptor (Qiagen). 1 mL of TRIzol per 250 mg of tissue was used for homogenization and RNA was isolated as per the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2023Quote: Two-week-old Arabidopsis seedlings of Col-0 wild type and trb1/2/3 triple mutans were used for DNA extraction using DNeasy Plant Mini Kit (QIAGEN). A total of 500 ng DNA was sheared with Covaris S2 (Covaris ...
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bioRxiv - Molecular Biology 2023Quote: ... cf-DNA quantified from various plasma or serum types using either MitoQuicLy or a silica-based membrane column DNA extraction kits (DNeasy, Qiagen). A total of 50 samples were analyzed (5 biofluids ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from adult ovaries of transgenic or wild-type zebrafish using the RNeasy Mini kit (QIAGEN, 74134) and reverse-transcribed using SuperScript IV reverse transcriptase (ThermoFisher ...
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bioRxiv - Biochemistry 2023Quote: ... A linearized PCR template was generated by digesting a wild-type Hrd1 plasmid with restriction enzyme and purified using a QIAquick PCR purification kit (Qiagen). For each region two separate PCR reactions were set up ...
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bioRxiv - Evolutionary Biology 2023Quote: RNA from HeLa cells transfected with either wild-type PADI2 (WT) or mutant PADI2 (with L642A, or L642A/W161A, or L642A/W161A/T159A) was extracted using RNeasy (Qiagen) according to the manufactureŕs instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Pathway analysis of genes adjacent to identified tissue and cell-type specific methylation blocks was performed using Ingenuity Pathway Analysis (IPA) (Qiagen) (90).
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bioRxiv - Molecular Biology 2024Quote: RNA isolation from leaf tissues of five-week-old non-transformed wild type and overexpressing transgenic lines (two replicates of wild type and two transgenic lines) were isolated as per manufacturer’s instruction (Qiagen, Germany). High quality RNA samples with 200 ng/μL concentration were outsourced for RNA-seq ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was isolated from two-week-old Villersexel wild type and the nog1dis mutant using the RNeasy kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... and RNA was then subjected to DNase I digestion and purified using a miRNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... and total RNA was extracted with QIACube using RNeasy kit with on-column DNase I treatment (Qiagen). For each sample ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... instrument according to the manufacturer’s protocol with on column for RNase-Free DNase I (Qiagen, Cat# 79254) treatment ...
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bioRxiv - Synthetic Biology 2019Quote: ... according to the manufacturer’s protocols including the on-column DNA digestion (RNase-free DNase I, Qiagen, 79254). RNA was reverse transcribed using either the QuantiTect Reverse Transcription Kit (Qiagen ...
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A novel transplantable model of lung cancer associated tissue loss and disrupted muscle regenerationbioRxiv - Cancer Biology 2020Quote: ... and DNase I treated using Trizol and purified on RNeasy Mini kit columns (Qiagen, Mississauga, ON, Canada). RNA was quantified using a NanoDrop Spectrophotometer (ThermoScientific ...
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bioRxiv - Neuroscience 2020Quote: ... with on-column DNase I digestion to remove genomic DNA using RNase-Free DNase Set (#79254, QIAGEN). The concentration of RNA was determined by Nanodrop 2000 ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was extracted with Trizol followed by DNAse I treatment and purification (RNeasy RNA purification kit,Qiagen).The purified RNA was quantified with Nanodrop and QC checked by Agilent Bioanalyzer.
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bioRxiv - Cancer Biology 2022Quote: ... according to the manufacturer’s instructions and treated with DNase I by using RNase free DNase set (Qiagen).
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bioRxiv - Microbiology 2022Quote: ... DNA extraction was performed on each piece using the following methods: (i) DNeasy Blood & Tissue kit (Qiagen, Hilden ...
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bioRxiv - Cell Biology 2022Quote: ... Condensin I was bound to Glutathione Sepharose 4B beads (Cytiva) in a 5 mL polypropylene column (Qiagen) using GST- tagged mSMC4 and eluted by PreScission Protease (Cytiva ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was purified and DNAse I treated according to the manufacturer’s instructions using RNeasy mini kits (Qiagen). For embryo inhibitor experiments ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative real-time PCR was performed using SYBR Green I fluorescence and Rotor-Gene Q cycler (Qiagen). Melting-curve analysis was done using Rotor-Gene Q series software version 2.1.0.
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bioRxiv - Immunology 2020Quote: Total RNA from ~300,000 OT-I cells per sample was collected with the RNeasy micro kit (Qiagen) with a 15-minute on-column DNase digestion (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... and 7 were isolated using a Plant RNeasy Mini kit with DNase I treatment (Qiagen, Hilden, Germany). Three biological replicates were prepared for each tissue type ...
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bioRxiv - Immunology 2021Quote: DNase I-treated total RNA was prepared from sorted T cells using RNeasy RNA isolation kit (Qiagen) and cDNA was synthesized using an iScript cDNA synthesis kit (Biorad) ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was isolated and DNase I treated using Qiagen RNeasy Plus Mini Kit (Qiagen, Valencia, CA). The RNA integrity was checked by Agilent Bioanalyzer 2100 (Agilent ...
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bioRxiv - Developmental Biology 2022Quote: ... In vitro transcribed mRNAs were treated with DNase I and purified using the RNeasy Mini kit (Qiagen). EnGen Spy Cas9 NLS protein (NEB ...
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bioRxiv - Genomics 2022Quote: ... DNA from I-73-1 and D308 was extracted using a ‘Genomic-tip 100/G’ kit (Qiagen) and long-reads sequenced with PacBio RS II at 100x coverage ...
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bioRxiv - Molecular Biology 2022Quote: ... Genomic DNA contamination was removed by treating the isolated RNA samples with RNase-free DNase I (Qiagen), followed by phenol/chloroform extraction and ethanol precipitation ...
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CDK5 activity in retinal pigment epithelium contributes to gap junction dynamics during phagocytosisbioRxiv - Cell Biology 2023Quote: ... according to the manufacturer’s instructions in which we performed the optional DNAse I (Qiagen, Valencia, CA, USA) incubation step ...
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bioRxiv - Microbiology 2023Quote: ... A total of 384 different conditions were included in the screen (JCSG Core Suite I-IV, Qiagen) and crystals obtained in 0.2 M disodium tartrate supplemented with 20% (w/v ...
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bioRxiv - Developmental Biology 2023Quote: ... extraction followed by clean up and on-column DNase I treatment using an RNeasy Mini Kit (Qiagen). cDNA was reverse transcribed using SuperScript III (Thermo Fisher ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... remaining cellular DNA was digested using 0.0164 Kunitz Units/µL DNase I in Buffer RDD (79254, Qiagen), RNA digestion was prevented by utilizing 0.533 U/µL RNasin® ribonuclease inhibitor (N2511 ...
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bioRxiv - Plant Biology 2024Quote: ... using the RNeasy Plant Mini with DNase I treatment according to the manufacturer’s instructions (Qiagen, Valencia, CA). One μg of RNA was used for a 20-µL first-strand cDNA synthesis reaction using High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: RNA was purified using the PureLink RNA mini kit with in-column DNAse-I digest (#79254, Qiagen) following the manufacturer’s instructions (#12183018A ...
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bioRxiv - Zoology 2021Quote: ... PCR amplifications were carried out in 10 μl reaction volumes containing 5 μl Type-it Microsatellite PCR Kit (Qiagen, Hilden, Germany), 0.5 μM of each primer ...
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bioRxiv - Microbiology 2019Quote: ... was used to amplify species specific 16S rRNA genes in order to identify and to quantify the relative abundance of 45 types of gut microbiota according to the manufacturer’s instructions (Qiagen, Hilden Germany) (Supplementary table 1) ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from the allantoic fluid of recombinant and wild type after the first and fifth passages in eggs using the QIAamp® Viral RNA Mini Kit (Qiagen). The real-time qRT-PCR was performed using SuperScript™ III Platinum™ One-Step qRT-PCR Kit to detect NDV M gene41 ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from the fifth leaf of wild-type and mutant plants using the RNeasy Plant Mini Kit (Qiagen, Germany). First-strand cDNA was synthesized from 500 ng total RNA using the PrimeScript RT reagent kit (Takara ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from transgenic seedlings carrying 35S promoter-driven wild-type RAPTOR1B/ raptor1b or RAPTOR1BS916A/raptor1b and control seedlings using a Qiagen RNeasy kit (Qiagen, 74104) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Genomic DNA was isolated from wild type (mCherry negative) and mCherry positive cells using Qiagen DNeasy Blood & Tissue Kit (Qiagen N.V.). DNA concentration was measured and calculations were made to prepare a genomic DNA mixture representing approximately 100 transduced cells and 3.79×104 wild type cells in a total of 250 ng DNA corresponding to approximately 3.8×104 cells ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was isolated from NPCs and their derived astrocytes (4 lines, n=3 per cell type) using a RNeasy mini kit (Qiagen, 74104). RNA samples were prepped using TruSeq® Stranded mRNA Library kit (Illumina ...
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bioRxiv - Genomics 2023Quote: Total RNA was extracted from frozen mammary tissue from wild-type mice at day six of pregnancy and purified with RNeasy Plus Mini Kit (Qiagen, 74134). Ribosomal RNA was removed from 1 μg of total RNAs and cDNA was synthesized using SuperScript III (Invitrogen) ...
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bioRxiv - Genetics 2023Quote: ... TPOX and FGA) and the Amelogenin (AMEL) gender-identification locus (24,25) using the Type-it Microsatellite PCR kit (Qiagen Gmbh, Hilden). The three multiplex PCRs and their respective primer pairs are given in Supplementary Table 1 ...
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bioRxiv - Genomics 2023Quote: Total RNA was extracted from frozen mammary tissue from wild-type mice at day six of pregnancy and purified with RNeasy Plus Mini Kit (Qiagen, 74134). Ribosomal RNA was removed from 1 μg of total RNAs and cDNA was synthesized using SuperScript III (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from wild type and RFX2 -/- LUHMES cells and column-purified using an RNeasy mini kit (Qiagen 74104) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The complete set of 15 loci were amplified in four separate 15 μL multiplex-PCR reactions containing 7.5 μL of 2X Master Mix (Type-It Microsatellite PCR kit, Qiagen, Venlo, Netherlands), 0.015-0.15 μL of unlabeled forward primer with tail (10 μM) ...
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bioRxiv - Developmental Biology 2024Quote: ... 10 katna1-/-MZ and one pool of 10 wild-type embryos (at 24 hpf) using the RNeasy Mini-kit (Qiagen, France) and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Genomic DNA of wild-type and KO mice was prepared from their tails using the DNeasy Blood & Tissue Kit (QIAGEN, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from whole frozen renal tissue from wild-type and mutant mice and purified with RNeasy Plus Mini Kit (Qiagen, 74134). Ribosomal RNA was removed from 1 μg of total RNAs ...
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bioRxiv - Plant Biology 2020Quote: ... The DNA in each total RNA sample was digested using DNase I (RNase-free DNase sets, Qiagen, CA). The RNA-seq library was prepared through the Lasy-Seq v1.1 protocol (Kamitani et al. ...