Labshake search
Citations for Qiagen :
451 - 500 of 746 citations for I TAC CXCL11 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Cytokine secretion assays were performed using Human Multi-Analyte ELISArray plate (Qiagen CMEH6321A). IL1β ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were prepared using the QIASeq Human Comprehensive Cancer Panel Kit (Qiagen #333515) and sequenced on an Illumina HiSeq 2500 or NovaSeq 6000 ...
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bioRxiv - Pathology 2019Quote: Total RNA of human podocytes was extracted per manufacturer’s instructions (Qiagen, Germantown, MD) and 1 μg RNA was used for cDNA synthesis (Transcriptor first strand cDNA synthesis kit ...
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bioRxiv - Genomics 2019Quote: We analyzed human and mouse Tug1 cDNA sequences with CLC Genomics Workbench (Qiagen) for open reading frames (ORFs) ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted from human cells with an RNeasy Mini kit (Qiagen). Northern blot was performed as described previously (Tafforeau et al. ...
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bioRxiv - Neuroscience 2022Quote: ... using commercially available primers for human FAAH (CpG Island 100530) (EPHS100530-1A, Qiagen). Methylation-sensitive (EPHS115450-1A ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was extracted from human thyroid tissues using miRNeasy Mini kit (Qiagen) according to the manufacturer’s instructions and as described [26] ...
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bioRxiv - Neuroscience 2023Quote: ... Human rRNA depletion was performed using the QIASeq FastSelect kit (Qiagen, Hilden, Germany). RNA sequencing library preparation used NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s recommendations (New England Biolabs ...
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bioRxiv - Immunology 2023Quote: DNA from human stool samples was extracted with DNeasy PowerSoil Pro kit (Qiagen) according to the manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2022Quote: Total RNA for human TSCs was extracted using RNeasy Micro kit (Qiagen, 74004) with on-column DNase digestion ...
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bioRxiv - Synthetic Biology 2023Quote: ... or primary human cells were extracted using the Qiagen RNeasy Micro Kit (Qiagen). cDNA was produced using the SuperScript IV VILO cDNA Synthesis Kit (Thermo Fisher) ...
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Cytokinin-promoted secondary growth and nutrient storage in the perennial stem zone of Arabis alpinabioRxiv - Plant Biology 2020Quote: ... including DNase I digestion via the RNase-Free DNase Set to remove traces of genomic DNA (Qiagen, Hilden, Germany). Quality and quantity of the isolated RNA was examined with the Fragment Analyzer (Advanced Analytical Technologies GmbH ...
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bioRxiv - Microbiology 2020Quote: ... and an on-column DNase I digest was performed with the Qiagen RNase-Free DNase Set (Qiagen, Hilden, Germany). RNA was eluted in 50 μl of RNase-free molecular biology grade water and DNA was removed by incubation with 1 μl TURBO DNase (2 U/μl ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA extractions were performed with the RNeasy extraction kit and genomic DNA traces were removed using DNAse I (Qiagen). RNA was eluted in 100 μL ...
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bioRxiv - Plant Biology 2019Quote: ... All qRT-PCR reactions were performed using SYBR Green I (QuantiFast® SYBR® Green PCR Kit, Qiagen, Germany) chemistry and ARF (ADP-Ribosylation Factor ...
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bioRxiv - Plant Biology 2019Quote: ... Residual genomic DNA was digested during RNA purification using an on-column DNAse I treatment (Qiagen, Germantown, MD, USA). The purified RNA was reverse-transcribed to synthesize first strand cDNA using SuperScript™ III Reverse Transcriptase (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The only modification to the protocol of the kit was the extension of the on column DNase I (QIAGEN) digestion to 30 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 mM GDPNP-Mg2+) were obtained at 4°C using 0.2 M Lithium nitrate and 20% PEG3350 (PEG Suite I, Qiagen) as precipitating agent ...
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bioRxiv - Developmental Biology 2022Quote: ... Genomic DNA was eliminated by both the gDNA eliminator column and on-column incubation with DNase I (79256, Qiagen). Sequencing libraries were constructed and sequenced at the National Cancer Institute’s Center for Cancer Research sequencing core facility using Illumina TruSeq® Stranded mRNA kit.
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bioRxiv - Developmental Biology 2022Quote: ... Total mRNA was isolated using the RNeasy kit according to the manufacturers’ instructions and digested with DNase I (QIAGEN) to remove genomic DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... Genomic DNA was eliminated by both the gDNA eliminator column and on-column incubation with DNase I (79256, Qiagen). RNA concentration and integrity was determined using RNA ScreenTape (5067-5576 ...
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bioRxiv - Molecular Biology 2022Quote: ... The libraries were built with 10.25 μl of DNA as starting material and using the GeneRead DNA Library I Core Kit (Qiagen, half of the volumes suggested by the manufacturer instructions were used ...
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bioRxiv - Synthetic Biology 2021Quote: ... RNeasy column with DNase I treatment used for RNA purification according to the manufacturer’s instructions (RNase Plus Mini Kit, Qiagen). Ribominus RNA was prepared using RiboMinus™ Eukaryote Kit v2 (Invitrogen) ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was isolated from biologically triplicate samples using miRNeasy micro kit with on-column DNase I digestion (Qiagen). RNA-seq libraries were prepared using the Ovation RNA-seq system v2 kit (NuGEN) ...
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bioRxiv - Immunology 2022Quote: Total RNA was extracted from cells and treated with DNase I according to the manufacturer’s instructions using Qiagen RNeasy Mini Kit (Qiagen). 100 to 1000 ng RNA was reverse-transcribed into cDNA in a total volume of 20 μl using SuperScript IV Reverse Transcriptase (ThermoFisher) ...
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bioRxiv - Neuroscience 2023Quote: ... Residual DNA contamination was removed by treating the spin column with 40 units of RNase-free DNase I (Qiagen) for 15 min at 23 °C before RNA elution ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then mechanically lysed and their RNA purified and treated with DNase I using an RNeasy kit according to the manufacturer’s instructions (Qiagen). RNA concentrations were assessed on a NanoDrop Lite spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was isolated from BMDMs using the RNeasy Mini kit with an on-column DNase I treatment according to the manufacturer’s instructions (Qiagen). RNA concentration was determined using a Qubit fluorometer (Qiagen ...
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bioRxiv - Plant Biology 2023Quote: ... extracted and rid of contaminant genomic DNA using RNeasy Plant Mini Kit and on-column DNAse I treatment (Qiagen), following supplier’s indications ...
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bioRxiv - Neuroscience 2023Quote: ... following the manufacturer’s instructions including DNase I treatment using RNase-Free DNase Set and on-column protocol (QIAGEN, USA). The isolated total RNAs were aliquoted for both RNA-seq and qRT-PCR.
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bioRxiv - Biophysics 2021Quote: ... Small interfering RNAs (siRNAs) targeting human AKR1C1-4 were synthesized by Qiagen (Valencia, CA). HepG2-RC cells (5×106 cells/well ...
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bioRxiv - Genetics 2021Quote: Human genomic DNA (gDNA) was extracted using the QIAamp DNA Mini Kit (QIAGEN, Germany) and quantified using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... pallidum and human RNase P gene (RNP) using a Rotor-Gene 6000 instrument (Qiagen) as previously described with modifications (11 ...
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bioRxiv - Immunology 2019Quote: RNA was extracted from 2×106 human PMNs using the RNeasy Mini Kit (Qiagen) as per manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... Human RAC2 3’UTR was amplified with One Step Ahead RT-PCR kit (Qiagen) from human mRNA using the following primers RAC2 3’UTR+ ...
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bioRxiv - Immunology 2021Quote: ... total RNA of CD8+ human T cells was extracted by miRNeasy Mini Kit (Qiagen), following the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and human islets were isolated using RNeasy Mini or Micro kits (Qiagen; Valencia, CA). Reverse transcription was completed with a High Capacity cDNA Reverse Transcription kit (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... rodent tissues and human samples using the Qiagen Blood and Tissue Kit (Qiagen, USA). The procedures prior to protein digestion were as follows ...
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bioRxiv - Neuroscience 2022Quote: Cultured human T cells after treatment were flash frozen in Buffer RLT (Qiagen, 79216) and kept in −80°C until processing ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA from cultured human cells were isolated using the QIAamp DNA kit (Qiagen) and genomic DNA from MEFs and mouse tissue samples were extracted using the DNeasy Blood and Tissue Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was isolated from human cells with an RNA purification kit (Qiagen, 74134) and genomic DNA was removed by genomic DNA binding columns in the kit ...
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bioRxiv - Genomics 2019Quote: Dissociated human islets cells were transfected with scramble siRNA (Cat# 1027284, Qiagen, Toronto, Canada) or siRNA from ThermoFisher Scientific against OGDHL (ID# s31422) ...
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bioRxiv - Genomics 2021Quote: cDNA was generated from 5μg of Human XpressRef Universal Total RNA (Qiagen cat # 338112) using Superscript III Reverse Transcriptase (Invitrogen cat # 18080093 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Total RNA was extracted from cultured human ECs using a RNeasy Mini kit (Qiagen). For reverse transcription ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... or human ABCB1-transfected MDR-19 cells using the RNeasy Mini extraction kit (Qiagen). First strand cDNA synthesis was performed on 500 ng of template RNA using MMLV reverse transcriptase (fc 10 units/µl) ...
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bioRxiv - Microbiology 2021Quote: The RTK RNAi library contained siRNAs targeting 56 human RTK genes (Qiagen, Dusseldorf, Germany), which included four different pairs of siRNAs for each gene ...
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bioRxiv - Neuroscience 2023Quote: Transfected iPSC-OPCs and post-mortem human MS samples were lysed in Qiazol (Qiagen), and RNA was isolated using a standard chloroform extraction and ethanol precipitation method ...
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Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: Bacterial and human cells were treated with RNAprotect cell or bacterial reagent (Qiagen, Germany) and stored at -80°C for up to 1 week ...
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bioRxiv - Cell Biology 2023Quote: CD14+ human monocytes were transfected with 200 nM siRNA using the HiPerfect system (Qiagen) as described previously ...
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bioRxiv - Physiology 2023Quote: Total RNA was isolated from human islets using the miRNeasy Mini Kit (Qiagen, Germany), according to the manufacturer’s instructions ...