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Citations for Qiagen :
451 - 500 of 3512 citations for Human Immunodeficiency Virus Reverse Transcriptase Protein HIV 1 Clade B IIIB since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... DNase-treated RNA (2μg) was then reverse-transcribed in a total volume of 20μl using the Omniscript Reverse Transcription Kit (Qiagen).
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bioRxiv - Physiology 2019Quote: ... Reverse transcription was carried out using 0.4 μg of total RNA as template (QuantiTect Reverse Transcription kit, QIAGEN). RT-qPCR analyses were performed using a Step-One-Plus cycler ...
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bioRxiv - Immunology 2021Quote: ... Five hundred nanograms of RNA was reverse transcribed into cDNA using the QuantiTect reverse transcription kit (Qiagen, # 205311). For one real-time reaction ...
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bioRxiv - Molecular Biology 2019Quote: ... 1µg of total RNA was subjected to reverse transcription with a QuantiTect Reverse Transcription Kit (Qiagen, Germantown, MD). The resulting cDNA was used for quantitative PCR using a Fast SYBR green master mix (Applied Biosystems ...
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bioRxiv - Genetics 2022Quote: ... RNA was treated with RNAse free DNAse prior to reverse transcription with the Quantitect reverse transcription kit (Qiagen). Quantitative RT-PCR was conducted in triplicate using a Quantitect SYBR Green PCR reagent (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... Reverse transcription was performed on 0.5 μg of total RNA using Quantitect Reverse Transcription Kit (Qiagen, Hilden, Germany), and 2 μL of the resulting cDNA was amplified by PCR using TaqMan Universal PCR Master Mix (Applied Biosystems ...
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bioRxiv - Physiology 2022Quote: ... RNA (2000 ng) was reverse transcribed to cDNA using the QuantiTect Reverse Transcription Kit (Cat No. 205311, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... FAF1 (Uniprot identifier Q9UNN5-1) and UBXN7 (Uniprot identifier O94888-1) were amplified from XpressRef Universal Total human RNA (QIAGEN, 338112) by RT-PCR (TaKaRa ...
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bioRxiv - Cell Biology 2020Quote: ... reverse transcribed to produce cDNA (QuantiTect, Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... For retrotranscription QuantiTect Reverse Transcription kit (QIAgen) was used to synthesize cDNA ...
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bioRxiv - Cell Biology 2019Quote: ... The QuantiTect Reverse Transcription Kit (QIAGEN, 205313) was used for cDNA synthesis including an additional step to eliminate genomic DNA contamination ...
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bioRxiv - Microbiology 2022Quote: ... or a QuantiNova Reverse Transcript Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and reverse transcription to cDNA (Omniscript, Qiagen) was conducted using 500 ng RNA per sample ...
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bioRxiv - Neuroscience 2022Quote: ... A QuantiTect® Reverse Transcription Kit (Qiagen) was used to generate cDNA ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... A QuantiTect Reverse Transcription Kit (Qiagen – 205311) was used to degrade genomic DNA and synthesise cDNA ...
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bioRxiv - Cell Biology 2023Quote: ... QuantiTect Reverse Transcription Kit (Qiagen, catalog #: 205311) was used to synthesize cDNA from total RNA ...
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bioRxiv - Immunology 2023Quote: ... The QuantiTect Reverse Transcription Kit (Qiagen, 205314) was used to synthesize cDNA according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ... and reverse transcription kit QuantiTect (205313, Qiagen) for cDNA generation ...
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bioRxiv - Genetics 2023Quote: ... employing the QuantiTect Reverse Transcription Kit (Qiagen) and the SYBR Fast Universal qPCR Kit (Kapa Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... using the QuantiTect Reverse Transcription Kit (Qiagen). Genomic DNA was eliminated through use of the gDNA Wipeout Buffer contained in the same kit and confirmed through PCR using the Avr9B-F/Avr9B-R primer pair (Table S4) ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription of 1µg RNA per case to complimentary (cDNA) was performed using a Quantitect Reverse Transcription Kit (Qiagen) with integrated genomic DNA removal ...
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bioRxiv - Bioengineering 2020Quote: ... Reverse transcription of RNA to cDNA was performed following directions and supplies from a QuantiNova Reverse Transcription kit (Qiagen) and a S100 thermal cycler (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... Reverse transcription was performed using the QuantiTect Reverse Transcription Kit with integrated removal of genomic DNA contamination (205311, QIAGEN), and resulting cDNA was amplified using an Applied Biosystems ViiA 7 Real-time PCR System ...
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bioRxiv - Molecular Biology 2020Quote: RNA was isolated using the Nucleospin RNA II kit (Macherey & Nagel) according to the manufacturer’s instructions and reverse transcribed into cDNA by using the QantiTect reverse transcription kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... From control and lesion specimens 500ng of total RNA were reverse transcribed using the Quantitect reverse transcription kit (Qiagen). Quality and purity of cDNAs was confirmed by Agarose gel Electrophoresis ...
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bioRxiv - Bioengineering 2023Quote: Extracted total RNA was reverse transcribed into cDNA using the QuantiTect Reverse Transcription Kit protocol (#205311, Qiagen, Germantown, MD). Pre-designed primers were purchased for FLRT2 (#Hs00544171_s1 ...
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bioRxiv - Immunology 2023Quote: ... 1 μg of RNA was pre-treated with gDNA Wipeout Buffer and reverse-transcribed to cDNA according to the manufacturer’s instructions using QuantiTect Reverse Transcription Kit (QIAGEN). Quantitative gene expression analysis was performed using the LightCycler®480 SYBR Green I Master (Roche ...
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bioRxiv - Genomics 2020Quote: The DNA of VLPs was extracted following the manufacture’s protocol for the QIAampMinElute Virus Spin Kit (QIAGEN). The resulting DNAs were quantified (Qubit ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from the sucrose-purified virus using the Qiagen DNeasy blood and tissue kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted from mock and virus infected Vero cells using the RNeasy mini kit (Qiagen, Germany). Next ...
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bioRxiv - Plant Biology 2020Quote: DNA from virus-infected plant tissues was extracted by DNeasy Plant Mini Kit (QIAGEN, Cat. No. 69104), and 500 ng of purified DNA was subjected to bisulfite treatment using EpiTect Plus DNA Bisulfite Kit (QIAGEN ...
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bioRxiv - Microbiology 2022Quote: The viral RNA of the egg-passaged virus was extracted using QIAamp Viral RNA Mini Kit (Qiagen). The extracted RNA was then reverse transcribed to cDNA using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs) ...
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bioRxiv - Immunology 2021Quote: ... Viral RNA from mice serum was extracted using QIAamp MinElute Virus Spin Kit (Qiagen, catalog no. 57704) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... For quantification RNA was extracted from 100 μl of virus preparation using RNeasy kit (Qiagen, Manchester, UK) and analysed by reverse transcription and quantitative PCR (qPCR).
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bioRxiv - Bioengineering 2020Quote: ... Viral RNA (vRNA) was isolated from blood plasma using the Qiagen QIAmp UltraSense Virus Kit (Qiagen #53704) in accordance with manufacturer’s instructions for 0.5 mL of plasma ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from cell-free virus-containing supernatants using the viral RNA extraction mini kit (Qiagen). cDNA synthesis was conducted using Superscript IV and the uni12-FluG primer (AGCGAAAGCAGG) ...
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bioRxiv - Immunology 2021Quote: ... RNA was isolated from 200 μL sample using the QIAamp MinElute Virus spin kit (Qiagen, Frederick, MD). For the qRT-PCR assay ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The RNA from the escaped virus was isolated using a QIAamp viral RNA mini kit (Qiagen, #52904), and the full-length HA was amplified using gene-specific primers and the OneStep RT-PCR kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: Total virus-RNAs were extracted from SK.N.SH infected cells at MOI 0.5 using RNeasy minikit (Qiagen, #74104). ISRE reporter assay was performed in STING37 cells plated in 24 wells plate (2×105 cells/well ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... Viral RNA was extracted from the concentrated virus with the QIAamp Viral RNA Mini Kit (Qiagen, 52904). The sgRNA sequences found in the genomic DNA and viral RNA samples were amplified by PCR (Agilent ...
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bioRxiv - Genomics 2022Quote: Viral RNA was extracted from 200 μl of clinical sample using QIAamp 96 Virus QIAcube HT (QIAGEN) according to the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2022Quote: ... Viral RNA (vRNA) was isolated from blood plasma using the Qiagen QIAmp UltraSense Virus Kit (Qiagen #53704) in accordance with manufacturer’s instructions for 0.5 mL of plasma ...
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bioRxiv - Microbiology 2023Quote: ... and cleared supernatants stored at −80°C until virus titration/ spin through a QIA shredder column (Qiagen). Viral titers were expressed as TCID50 units per gram source tissue.
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bioRxiv - Cell Biology 2019Quote: ... Total RNA was isolated following the manufacturer’s protocol and cDNA was synthesized from 1 μg RNA using the QuantiTekt Reverse Transcription kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using QuantiTect® Reverse Transcription kit (Qiagen, Germany).
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription was performed according to the manufacturer’s recommendations on 1 μg of RNA with the QuantiTect Reverse Transcription Kit (Qiagen), which includes a genomic DNA–removal step ...
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bioRxiv - Cell Biology 2019Quote: ... Total RNA was isolated following the manufacturer’s protocol and cDNA was synthesized from 1 µg total RNA using the QuantiTekt Reverse Transcription kit (Qiagen). The primers used for detecting Ccdc186 cDNA were:
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bioRxiv - Immunology 2022Quote: ... The complementary DNA (cDNA) was synthesized from 1 μg of total RNA using QuantiTect Reverse Transcription Kit (Qiagen: 205311) and quantitative PCR (qPCR ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA (1 μg) was treated with DNase and converted into cDNA using the QuantiTect Reverse Transcription kit (Qiagen), according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... Complementary DNA (cDNA) was synthesized from 1 ug of RNA using the QuantiTect reverse transcription kit (Qiagen, catalog #205311) following the manufacturer’s instructions ...