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Citations for Qiagen :
451 - 500 of 571 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... for 6 h at 55 °C followed by purification with a Qiaquick PCR Kit (Qiagen). Libraries were prepared using a MicroPlex Library Preparation Kit (Diagenode ...
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bioRxiv - Biophysics 2022Quote: ... the supernatant was incubated for 2 h at 4 °C with Ni2+-NTA-agarose (Qiagen) (20 mg of proteins/ml of resin ...
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bioRxiv - Bioengineering 2023Quote: ... Genomic DNA was extracted 72 h after transfection using a DNeasy Blood & Tissue Kit (Qiagen). Next-generation sequencing samples were prepared via two-step PCR following the protocol reported previously.55 Amplicon sequences were analyzed by CRISPResso 2.56
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bioRxiv - Bioengineering 2023Quote: ... Genomic DNA was extracted 72 h after transfection using a DNeasy Blood & Tissue Kit (Qiagen). Next-generation sequencing samples were prepared via the two-step PCR protocol that was reported previously.55 Amplicon sequences were analyzed by CRISPResso 2.
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bioRxiv - Bioengineering 2023Quote: ... Genomic DNA was extracted 72 h after transfection using a DNeasy Blood & Tissue Kit (Qiagen), and next-generation sequencing samples were prepared via the two-step PCR protocol that was reported previously.53 Amplicon sequences were analyzed by CRISPResso 2.54
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bioRxiv - Genetics 2024Quote: ... Proteins were harvested 60 h after infection and purified with Ni-NTA Superflow resin (Qiagen), anion-exchange chromatography (Source 15Q ...
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bioRxiv - Microbiology 2024Quote: 100nM NEAT1 GapmeRs (antisense complementary oligonucleotides that induce target degradation through RNAse H recruitment) (Qiagen) were added to cells for 24 hours prior to reactivation ...
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bioRxiv - Genetics 2021Quote: ... Cells were harvested 48 h post-transfection and RNA was extracted using RNeasy midi kit (Qiagen). Messenger-RNA was purified from bulk RNA using Dynabeads Oligo(dT)25 beads (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... 24 h later cells were harvested and RNA was isolated via the RNeasy Mini Kit (Qiagen). 20 μg isolated RNA was poly-A-selected using Dynabeads Oligo (dT)25 beads (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... and 24 h and used for total RNA extraction using RNeasy Plant Mini kit (Qiagen, Germany), and analyzed quantitatively and qualitatively using NanoVue plus spectrophotometer (GE health care ...
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bioRxiv - Immunology 2020Quote: ... The bacterial lysates were mixed for 1 h with 5 ml of Ni-NTA resin (Qiagen) that had been equilibrated with buffer A ...
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bioRxiv - Genomics 2021Quote: Cells were harvested and total RNA isolated 24 h after transfection using RNeasy Maxi kit (Qiagen) with on-column DNaseI digestion as per manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for 1 h at 55°C with 20 mg ml−1 proteinase K (Qiagen). Samples were treated for 10 min at 65 °C with 4 μl RNase A (100 mg ml−1 ...
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bioRxiv - Microbiology 2021Quote: ... and 5 h time points were immediately mixed with 2 x volume of RNAProtect® (Qiagen) using the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The lysates were then incubated for 1 h with nickel-nitriloacetic acid (Ni-NTA) beads (Qiagen) at the indicated temperatures (4°C or room temperature) ...
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bioRxiv - Neuroscience 2024Quote: ... unexposed or exposed with MnCl2 for 48 h) were extracted using the RNeasy mini kit (Qiagen) following manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatant was incubated for 2 h at 4°C while rotating with Glutathione superflow beads (Qiagen) for GST-tagged CMK-1 variants or nickel-nitrilotriacetic acid beads (Ni-NTA ...
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bioRxiv - Biochemistry 2024Quote: ... for 4 h at 37 °C and purified with PCR purification kit (QIAGEN, catalog no. 28106) per 20 μL IVT reaction ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was incubated for ~2 h rotating at 4°C with Ni-NTA Resin (Qiagen, 1018244) that had been washed with wash buffer (20mM Tris HCl pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... RNA from cells was isolated 48 h post transfection using the RNease Mini Kit (Qiagen, Manchester, UK) and diluted to 20 ng/μl ...
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bioRxiv - Plant Biology 2022Quote: ... the clear supernatant was incubated for 4 h with 1.5 ml Ni-NTA agarose (Qiagen, Hilden, Germany) previously washed with 6 ml of distilled H2O and equilibrated with 6 ml of binding buffer (150 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated from HD HSPCs 48 h after RNA transfection using the RNeasy Kit (QIAGEN) including a DNAse treatment step ...
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bioRxiv - Plant Biology 2024Quote: ... Each extraction step was carried out for 2 h in a TissueLyser II (Qiagen AB, Sollentuna, Sweden) at 6 s-1 at room temperature ...
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bioRxiv - Neuroscience 2021Quote: RNA was isolated from N2a cells upon antibody treatment for 18 h using the RNeasy Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Genes with significantly altered expression between PTPN23H/H and PTPN23+/+ IAT were analyzed with Ingenuity Pathway Analysis (Qiagen) to identify impacted pathways and candidate upstream drivers ...
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bioRxiv - Molecular Biology 2022Quote: ... coli grown for 16 h in 250 ml of 2×YT medium using the Plasmid Maxi Kit (QIAGEN) in accordance with the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... 4 °C for 1 h and purified by affinity chromatography using a 5 ml Ni-HP column (Qiagen). Flow through with pure AstaPo1 was collected and dialyzed overnight against the buffer containing 50 mM Tris pH 7.6 ...
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bioRxiv - Biophysics 2021Quote: ... The solubilized fraction was then incubated for 2 h with 300 µL of pre-equilibrated Ni-NTA resin (QIAGEN) in Ni-NTA buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA from h-iECs which have been expanded for 7 days was extracted using Rneasy Mini Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 40 min, 4°C) prior to incubation (1 h, 4°C) with 4.0 mL of Ni-NTA agarose (Qiagen) pre-equilibrated in the same buffer ...
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bioRxiv - Biophysics 2021Quote: ... Lysate was centrifuged for 1 h at 20,000 rcf and the supernatant collected and flowed through a column packed with Ni-NTA beads (Qiagen). The FRET construct was eluted with 50 mM NaH2PO4 ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting cells were treated with 5 ng ml-1 TGFβ1 for 72 h and total RNA was extracted by using TRIzolTM reagent and Qiagen RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... or 7-recrudescent populations as well as PruQ tachyzoites and alkaline-induced PruQ bradyzoites (pH8.2 media for 72 h) were purified using the RNeasay kit (Qiagen) according to manufacturer protocols ...
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bioRxiv - Cell Biology 2022Quote: Cells were seeded at 800,000 in 60 mm plates and 24 h later RNA was isolated (Qiagen RNeasy Kit). 1 ug of RNA was reverse transcribed (Applied Biosystem) ...
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bioRxiv - Cell Biology 2022Quote: Cells were seeded at 800,000 in 60 mm plates and 24 h later RNA was isolated (Qiagen RNeasy Kit). 1 μg of RNA was reverse transcribed (Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit) ...
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bioRxiv - Microbiology 2022Quote: ... 200 RPM for 2 h then approximately 1×109 CFU of bacteria were mixed at a 2:1 (vol:vol) ratio of RNAProtect (Qiagen) and incubated at room temperature for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... adjusted to pH 8.0 using 1M Tris-HCl pH 8.0 and incubated at room temp for 1 h with Ni-NTA Agarose beads (Qiagen). Beads were washed twice with PBS ...
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bioRxiv - Microbiology 2020Quote: Cells were harvested 24 h after treatment and RNA was extracted using the RNeasy 96 protocol (Qiagen, Valencia, CA). Gene expression for 22,238 probe sets of 12,490 genes was generated with U133A2.0 microarrays following the manufacturer’s instructions (Affymetrix ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were clarified by centrifugation for 1 h at 4°C at 10,000 rcf before being subjected to Ni-NTA (Qiagen) affinity purification following the manufacturer’s protocol.
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bioRxiv - Biochemistry 2022Quote: ... Culture medium was harvested 72 h post-transfection and secreted His-tagged proteins purified using Ni-NTA agarose (Qiagen) in batch mode ...
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bioRxiv - Physiology 2022Quote: ... Lysates were centrifuged at 100,000 x g for 1 h and the supernatant were affinity purified on Ni-NTA resin (Qiagen) by batch binding for 30 min-1h ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were incubated for 48 h post transfection and RNA was extracted with the RNeasy Mini kit (Qiagen, 74104) according to manufacturer instructions with sample concentrations assessed via NanoDrop ...
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bioRxiv - Microbiology 2024Quote: ... DNA on the exterior of filtered capsids was digested for 1 h at 25°C with 20.3 units DNase (79254; Qiagen) supplemented with 10 mM MgCl2 ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 µM A740003 or both BzATP and A740003 together (A740003 was pre incubated for 1 h before adding BzATP) 24 hours later RNA was extracted using the miRNeasy mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... The pellets were resuspended with OMV buffer and re-pelleted again by centrifugation at 200,000 g for 2 h at 4°C and lysed with Qiazol followed by RNA isolation with the miRNeasy kit (Qiagen) to obtain total RNA including the small RNA fraction ...
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bioRxiv - Molecular Biology 2020Quote: ... plasmids containing qPCR amplicon sequences were linearized by digestion with SmaI for 1 h and purified using the QIAquick PCR purification kit (Qiagen). Linearized plasmids were quantified using spectrophotometry ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from mammalian cell culture after 48 h of pulsatile light activation using RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... including miRNA was isolated from brains of E11 mouse fetuses (3 biological replicates) at 2 h after irradiation using the miRNeasy Mini Kit (Qiagen). RNA was subsequently processed for hybridization to GeneChip miRNA 4.0 microarrays (Affymetrix ...
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bioRxiv - Bioengineering 2020Quote: ... The PCR fragments were incubated with DpnI for 2 h at 37°C to degrade the template plasmids before being purified using a PCR purification kit (Qiagen). The purified PCR fragments were digested overnight at 37°C using NheI and XhoI and ligated overnight at 16°C to backbone vector pYZ125 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in either glutamine-containing or glutamine-free medium for 24 h and then used for total RNA isolation using the RNeasy kit (Qiagen). Sample preparation for analysis and processing of the arrays were performed using the protocol provided by Agilent Technologies ...