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Citations for Qiagen :
451 - 500 of 502 citations for Arsenazo I Trisodium Salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... tissues were ground in liquid nitrogen using a mortar and pestle and RNA were extracted and subsequently treated with DNAse I using the Plant RNeasy Mini extraction kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was extracted from homogenized samples using a Qiagen RNeasy plant RNA extraction kit with on-column DNase I (Qiagen) digestion following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The transcribed RNA was treated with Turbo DNase I and antarctic phosphatase to remove template DNA and purified by RNeasy MinElute Cleanup Kit (QIAGEN). The concentration was determined by Quibit microRNA Assay Kit ...
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bioRxiv - Cell Biology 2022Quote: ... DNA was removed by passing the lysate through the gDNA eliminator column and by an additional on-column DNase I treatment (Qiagen) before the elution ...
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bioRxiv - Plant Biology 2021Quote: ... RNA sample was treated with DNase I to remove genomic DNA and retro transcribed into cDNA using Quantitect Reverse Transcription Kit (Qiagen).
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bioRxiv - Immunology 2021Quote: ... cDNA was quantified using 96-well PCR array analysis on a PAMM-150ZA plate (Cytokines & Chemokines) and PAMM-016ZA plate (Type I Interferon Response) (both Qiagen). Quantitative real time-PCR (QRT-PCR ...
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA was extracted from each placenta using the Qiagen RNeasy Midi kit and DNase treated with DNase I (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... The aqueous phase was collected and the remainder of the RNA extraction procedure was performed using an RNeasy Mini Kit with on-column DNase I treatment (Qiagen) according to manufacturer instructions ...
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bioRxiv - Microbiology 2020Quote: ... for various immune genes were used to amplify cDNA collected from lungs using QuantiNova reverse transcription and SYBR Green I PCR kits (Qiagen). The ΔΔCq method was used with normalization within sample on GAPDH (ΔCq ...
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bioRxiv - Microbiology 2020Quote: ... The PCR fragment was then digested with Dpn I at 37 °C for 1 h and purified using the QIAquick PCR purification kit (Qiagen). To prepare the competent SW102 containing the pSMART BAC-T7-scv2-replicon ...
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bioRxiv - Microbiology 2021Quote: ... virion RNA was purified using the RNeasy Mini Kit with on-column DNase digestion with RNase-Free DNase I (Qiagen). The copies/μL in the virion standard were estimated by triplicate measurements using the Abbott RealTime SARS-CoV-2 assay (Abbott m2000 Molecular Platform) ...
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bioRxiv - Neuroscience 2022Quote: The isolation of total mRNA was performed with the RNeasy Micro Kit and treated with Rnase free Dnase I (Qiagen). 500ng were used to synthesize cDNA with the SuperScript III Reverse Transcriptase Synthesis Kit (Invitrogen) ...
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bioRxiv - Physiology 2024Quote: ... The resulting cRNA probe was digested with DNase I and purified with the RNeasy MinElute cleanup kit (Qiagen, Hilden, Germany).
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bioRxiv - Microbiology 2023Quote: ... was used for total RNA extraction according to the manufacturer’s protocol which included a first DNase treatment using the On-column DNase I digestion kit (Qiagen, Switzerland). Extracted total RNA was subjected to a second DNase treatment using Promega RQ1 at 1 unit/μg of RNA ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was digested on-column for 15 min at room temperature using the RNase-free DNase I Set (Qiagen). First-strand complementary DNA (cDNA ...
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bioRxiv - Bioengineering 2023Quote: ... followed by three rounds of washes with sterile PBS + PI for 45 mins, then subjected to one round of incubation with DNase I (4716728001, MilliporeSigma) + RNase A (19101, Qiagen) + PI for 72 hours ...
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Accumulation of TCR signaling from self-antigens in naive CD8 T cells mitigates early responsivenessbioRxiv - Immunology 2023Quote: 1 × 105 CD8+ CD44LO CD62LHI Qa2HI OT-I GFPLO and GFPHI cells from three biological replicates were sorted into RLT Lysis Buffer (Qiagen) containing 1% 2-mercaptoethanol ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA from mouse embryos were isolated using RNeasy plus micro kit with on-column DNase I digestion method (QIAGEN). cDNA libraries were produced by using an Ovation RNAseq amplification kit Ver ...
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bioRxiv - Cell Biology 2023Quote: ... BioConcept) in TAE buffer (3-07F03-I, BioConcept) and products were extracted using the QIAquick Gel Extraction Kit (28706, Qiagen) and Sanger sequenced by Microsynth (Balgach ...
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bioRxiv - Cancer Biology 2023Quote: Investigation of the cell cycle related genes in LoVo cell line on treatment with FA FOS I was performed as described above by using the synchronized and 24 h FA FOS I (198 µg/ml) treated cells profiled by using RT2 Prolifer PCR Array- Human Cell cycle from Qiagen. The data was analysed by using Gene globe platform and reactome pathway analysis.
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bioRxiv - Pathology 2023Quote: ... After DNase I digestion and removing the ribosomal RNAs (rRNAs) from total RNA using the QIAseq FastSelect-rRNA Plant Kit probe (QIAGEN), several Adenine bases were tailed at the 3’ end of the remaining RNAs according to Liefting et al (47) ...
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bioRxiv - Plant Biology 2023Quote: ... patens RNA was extracted and rid of contaminant genomic DNA using RNeasy Plant Mini Kit and on-column DNAse I treatment (Qiagen), following supplier’s indications ...
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bioRxiv - Plant Biology 2024Quote: RNA was isolated with the QIAGEN RNeasy kit and Total RNA was treated with RNase-free DNase I set (QIAGEN). RNAseq libraries were prepared and up to 20 million 150bp paired-end sequences were collected by Illumina-sequencing by Novogene (Uk) ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR product was treated with 40U Dpn I for 1 hour and was purified by QIAquick PCR Purification Kit (QIAGEN). The purified DNA was quantified by Qubit dsDNA BR kit (Invitrogen).
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bioRxiv - Pathology 2024Quote: Total RNA was extracted from cell lysates using EZNA RNA extraction kit I or from blood samples using QIAamp Viral RNA Mini Kit (Qiagen). Absolute quantification of positive strand gRNA was performed through one-step RT-qPCR ...
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bioRxiv - Microbiology 2024Quote: ... 6×105 cells were seeded onto type I collagen-coated 6-well plates and transfected with 200 ng of viral DNA using Attractene Transfection Reagent (Qiagen). Two days later ...
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bioRxiv - Biophysics 2024Quote: ... or Sepasol-RNA I Super G (nacalai tesque, Kyoto, Japan) and purified using Qiagen RNeasy mini kits (Qiagen, Hilden, Germany) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Reverse transcription and PCR I were performed in 384-well plates pre-loaded with 3 µL of Vapor-Lock (Qiagen). Cell lysis buffer and reverse transcriptase mix (0.4 µL/well ...
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bioRxiv - Genomics 2024Quote: Total RNA was extracted from around 100,000 cells using column purification with removal of genomic DNA using DNAse I (RNEasy Micro Kit, Qiagen, 74004). After Reverse Transcription (iScrpitTM ...
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bioRxiv - Biochemistry 2024Quote: ... DNase digestion was performed by adding 80 µL of DNase solution (10 µL RNase-free DNase I stock (Qiagen #79254) plus 70 µL Buffer RDD (Qiagen #79254) ...
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bioRxiv - Genomics 2024Quote: ... An additional DNase treatment was carried out by adding 2.5µL of DNase I and 10µL of RDD buffer from RNase-Free DNase Set (Qiagen; Cat. No. 79254), and 1µL of Recombinant RNasin RNase Inhibitor (10,000U ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was isolated from batches of embryos, ICM or TE (n = 20) using PicoPur Arcturus (Excilone, France) with a DNase I (Qiagen, Germany) treatment as recommended by the supplier ...
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bioRxiv - Cell Biology 2021Quote: ... treated with DNase I at 37°C for 30 min and finally cleaned with the miniElute kit (QIAGEN, Catalog Number: &28004). For next-generation sequencing ...
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bioRxiv - Cancer Biology 2020Quote: ... The ligated products were size-selected (AMPure Beads, Agencourt, Beverley, MA) and amplified (GeneRead DNA I Amp Kit, Qiagen, Mississauga, ON); then the regions of interest were captured using biotinylated DNA baits (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2022Quote: ... residual genomic DNA contamination was removed by an on-column DNAse digestion performed using the RNAse-free DNAse I kit (Qiagen #79254) following the manufacturer’s protocol at 37°C for 1 hr ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Total RNA was extracted using an RNeasy Mini kit with on-column DNase I digestion for 15 min (Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: Sequencing libraries were prepared by the University of Chicago Genomics Facility from DNase I treated RNA using Qiagen FastSelect (Qiagen #334215) and Illumina Stranded mRNA Prep (Illumina #20020595 ...
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bioRxiv - Neuroscience 2024Quote: ... followed by extraction of total RNA using a GenElute™ Mammalian Total RNA Miniprep Kit and on-column DNase I treatment (Qiagen). The RNA integrity number and concentration were assessed using a Fragment Analyzer (Agilent 5400) ...
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bioRxiv - Genomics 2020Quote: ... The DNA-seq libraries were constructed from 500 ng of size selected DNA with the GeneRead DNA library I core kit (Qiagen, cat #180434) and GeneRead Adapter I set B (Qiagen ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was treated with DNase I (Bangalore Genei, Bangalore, India) as described previously [28] and cleaned using RNeasy mini kit (Qiagen, Hilden, Germany). RNA was quantified by nano-spectrophotometer (Eppendorf ...
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bioRxiv - Microbiology 2022Quote: ... Dual-index barcodes and adapters were added to amplified products through a second-round of PCR using the QIAseq 16S/ITS 96-Index I array (Qiagen, Hilden, Germany). Cycling conditions were ...
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bioRxiv - Immunology 2022Quote: RNA from sorted Treg and Tconv cells was extracted using QIAzoland miRNeasy Micro Kit combined with recommended RNase-free DNase I treatment (all from Qiagen, Hilden, Germany). The quality of extracted RNA was assessed using Bioanalyzer and RNA 6000 Nano Kit (Agilent) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were lysed using 1 mg/mL lysozyme dissolved in TE buffer and purified using RNeasy Mini Kit with on-column DNA digestion with DNase I (Qiagen, Germantown, MD) following manufacturers’ instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA removal was performed by applying 80 μl of DNase I working solution (10 μL DNase stock + 70 μL 1x RDD buffer; RNase-Free DNase Set, Qiagen, Hilden, Germany) to the column and incubated for 15 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Clarified cell lysate was processed using a Qiagen RNeasy kit and in-column treatment with a DNase I kit (Qiagen, Germantown, MD) for purification of total RNA ...
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bioRxiv - Plant Biology 2023Quote: ... was treated with RNase-free DNase I to remove residual genomic DNA and cleaned using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... from which total RNA was extracted by following an established protocol using the RNeasy mini-kit and an RNase-free DNase I solution (Qiagen, Germantown, MD) for in-column gDNA removal (24) ...
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bioRxiv - Microbiology 2024Quote: ... 1×105 cells were seeded onto type I collagen-coated 24-well plates and transfected with 200 ng of viral genome using Attractene Transfection Reagent (Qiagen, Hilden, Germany). One day post-transfection ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from subconfluent cells at low passage using miRNeasy Mini Kit with DNAse I digestion (Qiagen 217400 and 79254). RNA was reverse transcribed with iScript Reverse Transcription Supermix (Bio-Rad 1708841) ...
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bioRxiv - Plant Biology 2022Quote: ... and 1:10 dilution of cDNA was used to quantify genes using SYBR Green I (QuantiFast® SYBR® Green PCR Kit, Qiagen, USA). ADP-Ribosylation Factor 1 (AB050957.1 ...