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Citations for Qiagen :
451 - 500 of 1228 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Samples stored in TRI reagent® were defrosted and the RNA in the aqueous phase of the phenol-chloroform extraction was purified using the RNeasy Mini Kit (Qiagen). RNA concentrations were determined from the absorbance of the sample at 260 nm using a Nanodrop spectrophotometer and reverse transcription was performed using Applied Biosystems™ TaqMan™ Reverse Transcription Reagents following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... and total RNA from the cells was extracted by phase separation with chloroform and subsequently using the RNeasy Mini Kit (Qiagen). RNA-Seq libraries were prepared using TruSeq RNA library Prep Kit v2 (Illumina ...
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bioRxiv - Synthetic Biology 2020Quote: Cells were grown in triplicates on JMM 40 mM glycine and 40 mM pyruvate till mid-log phase and 1-2 mL of cultures were harvested and stabilized directly by RNA Protect Bacteria Kit (Qiagen). Cells were then lysed using lysozyme and beating with glass beads in a Retschmill (MM200 ...
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bioRxiv - Microbiology 2021Quote: ... 300μL of the aqueous phase was used for RNA extraction using EZ1 RNA tissue mini kit and EZ1 advanced XL robot (both from Qiagen). Quantitative RT-qPCR were performed using primers previously described by Dias de Melo et al [15] using QuantiNova SYBR® Green RT-PCR Kit (Qiagen ...
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bioRxiv - Developmental Biology 2021Quote: ... aqueous phase from the Trizol reagent and proceeded with RNA mini-prep using the RNeasy Micro Kit (Qiagen Cat. 74004). We next took eluted RNA and proceeded with reverse transcription to create cDNA libraries for RT PCR ...
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bioRxiv - Genomics 2021Quote: ... Exactly 600 μl of the aqueous phase was used as starting material on the QIACube System (QIAGEN, Cat. No.: 9001882) to purify DNA according to the manufacture instructions using the DNeasy PowerWater Kit® protocol ...
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bioRxiv - Microbiology 2023Quote: ... total RNA was extracted from triplicate samples at mid-log phase growth when succinate concentrations reached approximately 25 mM using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... according to manufacturer’s instructions and the resultant aqueous phase was mixed (1:1) with 70% RNA-free ethanol and added to Qiagen Rneasy mini kit columns (Qiagen) and the kit protocol was followed ...
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bioRxiv - Molecular Biology 2023Quote: ... The reaction was mixed with an equal volume of chloroform/isoamyl alcohol (24:1) and separated with a phase-lock tube (Qiagen) by centrifugation (14000 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... The aqueous phase containing RNA was transferred to a new tube for extraction and clean-up using the RNeasy Mini Kit (QIAGEN) with an on-column DNase digestion as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: Purification of the DNA was performed by applying the aqueous phase directly to a DNeasy Blood & Tissue Prep Kit (Qiagen) purification column and following the recommended protocol for washing and elution ...
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bioRxiv - Microbiology 2023Quote: ... A volume of 1:1 of 70% ethanol was mixed with the upper phase before transfer on column (RNeasy Mini kit, Qiagen), and RNA was extracted according to manufacturer directions ...
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bioRxiv - Microbiology 2023Quote: Three individual colonies of WT and Pcu-shc::Δshc growth with and without 25 µM cumate were cultured to early exponential phase and total RNAs were extracted by Qiagen RNeasy Mini Kit as described previously (44) ...
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bioRxiv - Neuroscience 2022Quote: ... Tubes were spun for 15 minutes and the RNA in the aqueous phase was precipitated with 100% ethanol and transferred into an RNeasy column (Qiagen). 30μl of RNA per sample were collected and the concentration and purity were determined using a Nanodrop 1000 (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... After chloroform incubation and centrifugation per manufacture instructions RNA-containing phase was diluted with 70% ethanol 1:1 and RNA isolation was performed using RNeasy Mini Kit (Qiagen). For tissues ...
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bioRxiv - Cell Biology 2023Quote: ... The aqueous phase was separated and added to an equal volume of 70% ethanol before using the RNeasy minikit (Qiagen) to isolate RNA following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and total RNAs containing SINEUP-GFP RNAs at the aqueous phase were extracted by using an RNeasy mini Kit (Qiagen). The RNA was then digested with 4 U of TURBO DNase (TURBO DNA-free kit ...
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bioRxiv - Physiology 2023Quote: ... was set within the exponential phase of the PCR using the Rotor-Gene Q Series software (v.2.3.1, Build 49, Qiagen, Hilden, Germany). The PCR product was quantified using the 2−ΔΔCt method ...
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bioRxiv - Biochemistry 2022Quote: ... Imidazole pH 8.0 was added to the detergent phase to a final concentration of 15 mM and the mixture was incubated with Ni-NTA agarose beads (Qiagen) for 1 hour at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Standard curves were generated using known amounts of purified bacterial genomic DNA (gDNA) extracted from stable phase cultures using the DNeasy PowerSoil Pro Kit (Qiagen). Purified gDNA was quantified via nanodrop and used as stock for a 10-fold serial dilution standard curve ...
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bioRxiv - Molecular Biology 2023Quote: ... according to manufacturer instructions and the resultant aqueous phase was mixed (1:1) with 70% RNA-free ethanol and added to Qiagen Rneasy mini kit columns (Qiagen) and the kit protocol was followed ...
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bioRxiv - Bioengineering 2022Quote: ... the aqueous phase was mixed with ethanol in a 1:1 ratio and was added to RNeasy spin columns (Qiagen) for RNA purification ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We collected the upper phase and added 550 µL (or 1100 µL) of ethanol before transferring 700 µL into a RNeasy Mini column (Qiagen). We then centrifuged for 15s at 11,000g ...
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bioRxiv - Biochemistry 2024Quote: ... 5×106 cells from an exponential growth phase were washed once in PBS and RNA was isolated using the QIAGEN blood RNA isolation kit (Qiagen) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: Bacterial cultures (7 ml) from the mid-exponential phase (OD600 = 0.5 to 0.6) were harvested and treated with RNAprotect reagent (Qiagen, Germantown, MD), and the cell pellet ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA was extracted using TRIzol Reagent followed by chloroform phase separation and processing with the RNeasy Micro kit (Qiagen). The quality and concentration of RNA were assessed using NanoDrop and TapeStation ...
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bioRxiv - Molecular Biology 2021Quote: ... Total DNAs were extracted from the aqueous phase/ethanol solutions according to the manufacturer’s protocol using the DNAEasy Blood and Tissue Kit (Qiagen, Germantown, MD). Total DNA concentrations and purities were measured using NanoDrop (Thermo Fischer ...
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bioRxiv - Molecular Biology 2022Quote: ... The upper phase was transferred to RNeasy spin columns and total RNA was extracted using mini-RNeasy kits (Qiagen, Courtabœuf, France) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was extracted once with 350 μL Phenol:Chloroform:Isoamyl Alcohol (25:24:1) in a phase-lock tube (Qiagen cat. no. 129046) and centrifuged 5 min at 16,000xg followed by extraction with 350 μL chloroform ...
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bioRxiv - Molecular Biology 2022Quote: RNA was extracted from liquid nitrogen-frozen cell pellets from 20 mL cultures grown to early stationary phase using the RNeasy Plant Mini Kit (Qiagen, Germany). Immediately after extraction ...
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bioRxiv - Bioengineering 2021Quote: ... sludge samples were collected at the end of the aerobic phase periodically and the genomic DNA was extracted using the DNeasy PowerSoil Pro Kit (Qiagen, USA). The extracted DNA was sent to University of Connecticut-MARS facility for PCR amplification and sequencing targeting the V4 region using the primers 515F (5’-GTGCCAGCMGCCGCGGTAA-3’ ...
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bioRxiv - Cancer Biology 2023Quote: ... The upper aqueous phase was then transferred to a new tube and RNA was then further purified using the RNeasy Kit (Qiagen, 74104) and or PureLink™ RNA Mini Kit (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... and the RNA-containing aqueous phase was subjected to a column purification kit using RNeasy Mini RNA extraction kit (Qiagen, USA). RNA was DNase treated ...
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bioRxiv - Microbiology 2023Quote: ... The resulting aqueous phase was subjected to the final round of purification using the DNeasy Blood & Tissue Kit (Qiagen Cat#69506) with a final elution volume of 50 μl ...
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bioRxiv - Immunology 2022Quote: ... 350 µL of aqueous phase was transferred into a 2 mL reaction tube and subjected to the QIAcube (QIAGEN, Hilden, Germany) for RNA isolation ...
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bioRxiv - Microbiology 2024Quote: ... RNA samples were initially preserved with 1 mL of activated sludge (i.e., suspended phase) in 9 mL of LifeGuard Soil Preservation (Qiagen, Hilden Germany). This solution was thawed and centrifuged (16,000 g for 1-minute ...
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bioRxiv - Microbiology 2023Quote: ... genomic DNA was extracted from initial and exponential growth phase cultures grown on different N sources using a DNeasy PowerSoil Pro DNA isolation kit (QIAGEN, Germany) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... RNA extraction was performed first by phase separation with chloroform (200ul per 1ml of trizol) and purified using a column-based procedure (Qiagen 74104) with DNAse I treatment.
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bioRxiv - Genetics 2022Quote: ... The aqueous phase was mixed with an equal volume of 100% ethanol and transferred to an RNeasy MinElute spin column (Qiagen, 74104) for purification according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... log-phase cells in the range of 1–2 × 108 cells using the Qiagen DNeasy® Blood and Tissue kit (Qiagen). We resuspended the genomic DNA in 10 mM Tris-HCl pH 8.5 and stored it at 4° until submission to the McDonnell Genome Institute at Washington University in St ...
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bioRxiv - Microbiology 2022Quote: ... incubated in in HS + TMA at 37 °C were harvested at late-exponential phase (OD600 ∼0.8) for genomic DNA extraction using the Qiagen blood and tissue kit (Qiagen, Hilden, Germany). The concentration of genomic DNA was measured using a Nanodrop One Microvolume UV-Vis Spectrophotmeter (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... and the aqueous phase containing the RNA was transferred to a new tube for RNA isolation using the QIAcube System (Qiagen, #9001292) and RNeasy Mini Kit reagents (Qiagen ...
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bioRxiv - Genetics 2024Quote: ... The lysate was then centrifuged for 5 minutes at 12,000 x g at 4°C and the clear supernatant transferred to a MaXtract High Density phase lock tube (Qiagen, UK). After 5 minutes of incubation at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was reverse-transcribed with the miScript II RT Kit (Qiagen) and 20 ng of cDNA used for qPCR with the human miFinder miRNA Array (Qiagen) ...
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bioRxiv - Plant Biology 2022Quote: ... Tissue was homogenized in a TissueLyser II (Cat No.: 85300, QIAGEN) using 3-mm steel beads (Cat No. ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Then the samples were lysed by Tissue lyser II (QIAGEN, Germany) at 30 Hertz for 5 minutes ...
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bioRxiv - Plant Biology 2020Quote: ... Flower samples were homogenized in liquid nitrogen using TissueLyser II (Qiagen) in 2 mL Eppendorf tubes containing ceramic beads ...
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bioRxiv - Genomics 2020Quote: cDNA synthesis was done using miScript II RT Kit (Qiagen: 218160) followed by qRT-PCR with miScript SYBR Green PCR Kit (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... followed by reverse transcription with miScript II RT Kit (QIAGEN, 218160). RT-qPCR was performed using the miScript SYBR Green PCR Kit (QIAGEN ...
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bioRxiv - Cancer Biology 2020Quote: ... and gel purified using QIAEX II Gel Extraction Kit (20021, Qiagen). The annealed oligos were ligated into the gel purified vector ...