Labshake search
Citations for Qiagen :
451 - 500 of 2342 citations for 3 1 Pyrrolidinylmethyl phenyl magnesium bromide solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was briefly incubated (approximately 5 min) with 3 ml Ni-NTA Agarose (QIAGEN) before flow-through was collected ...
-
bioRxiv - Physiology 2024Quote: ... 3 mM benzamidine) with a steel bead7,17 using the TissueLyser II bead mill (Qiagen, USA) for 1 min at 30 Hz.
-
bioRxiv - Plant Biology 2024Quote: ... Leaf samples were collected in the well containing a 3 mm tungsten carbide bead (Qiagen) and 100 µl extraction buffer (200 mM Tris-HCl ...
-
bioRxiv - Pathology 2024Quote: ... the stock 3’DIG-labelled probes (scrambled (included in the miRCURY LNA Control Set - Qiagen); miRNA-34a custom probe YD00618250-BEG ...
-
bioRxiv - Cancer Biology 2024Quote: RNA library construction was conducted by using QIAseq UPX 3’ transcriptome kit (Qiagen, catalog# 333088). 7ng purified RNA for each sample (n = 4 per group ...
-
bioRxiv - Cell Biology 2020Quote: ... The resulting solution was transferred to a QIA shredder spin column (Qiagen Inc., Valencia, CA, USA) and centrifuged at 15,000 rpm for 2 minutes at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... and the DNA removed by in-solution DNAse digestion (Qiagen’s RNase-Free DNase Set, #Cat 79254) for 30 minutes at room-temperature ...
-
bioRxiv - Microbiology 2021Quote: ... and 50 µl of SL solution (Part of DNeasy UltraClean Microbial Kit; Qiagen, Cat #12224-250) was added ...
-
bioRxiv - Cancer Biology 2020Quote: Whole bone marrow cells were subjected to red blood cell (RBC) lysis (RBC lysis solution, Qiagen) and resuspended in IMDM media containing 10% FBS and 1% penicillin-streptomycin ...
-
bioRxiv - Microbiology 2021Quote: ... pH 8.0) containing 15 mg/mL of lysozyme and proteinase K solution (20 mg/mL, Qiagen), followed by an incubation with vortex and lysis steps described in the kit ...
-
bioRxiv - Microbiology 2021Quote: ... Third shoot leaves from wild and mesocosm plants were placed in DNeasy PowerSoil C1 solution (Qiagen) and sonicated for 3 minutes at 1.2 kHz in a sonicator bath ...
-
bioRxiv - Microbiology 2020Quote: ... crystals were flash frozen (100 °K) in the above reservoir solution supplemented with 10% glycerol (QIAGEN). The diffraction data set for complexes of Brd4(BD1 ...
-
bioRxiv - Cancer Biology 2021Quote: ... the optional on column DNase digestion was performed by adding 80 μl DNase I solution (QIAGEN) to the column membrane and incubating 15 minutes at room temperature ...
-
bioRxiv - Microbiology 2023Quote: ... 1.0mL of treated and untreated culture were added directly to 2mL of RNA protect solution (Qiagen), and RNA was extracted with the RNeasy Mini Kit (Qiagen ...
-
bioRxiv - Plant Biology 2023Quote: ... the solution was transferred to a new tube and purified using an RNeasy Mini kit (Qiagen). While the above extraction method was used for Nepenthes ...
-
bioRxiv - Genetics 2023Quote: ... Contaminating DNA in the RNA solution was digested using RNase-Free DNase Set (Qiagen, Hilden, Germany). The isolated RNA was purified using an RNeasy Kit (Qiagen) ...
-
bioRxiv - Cancer Biology 2024Quote: Frozen blood was thawed and resuspended in red blood cell lysis solution (Qiagen Inc., Valencia, CA). White blood cells were removed by centrifugation at 2000g for 5 mins and repeated until white blood cells were depleted ...
-
bioRxiv - Genomics 2022Quote: ... 300 µL lysis solution (LSDNA, RevoluGen) and 20 µL of 20 mg/mL Proteinase K (Qiagen) was added to the partly-lysed cells and incubated at 56 °C for 20 min ...
-
bioRxiv - Microbiology 2022Quote: ... We then put the solution through an extraction (Qiagen DNeasy Blood/Tissue Extraction Kit, Qiagen #69504) using the instructions provided by the kit ...
-
bioRxiv - Cell Biology 2023Quote: ... The cells sediment was added to 2 mL Red Blood Cells Lysis Solution (Qiagen, Duesseldorf, Germany) at room temperature for 5 min to remove red blood cells ...
-
bioRxiv - Microbiology 2023Quote: ... Each reaction contained 5 μL DNA solution and PCR mixture with Taq Type-it (Qiagen®). PCR products were analyzed by capillarity electrophoresis on an ABl3130xl sequencer ...
-
bioRxiv - Immunology 2023Quote: ... A second round of PCR was performed to generate uniquely barcoded sequencing libraries using the barcode primer plate included in the kit (17μL of working mix which include 12.5μL QIAGEN 2x Multiplex PCR master mix, 2.5μL QIAGEN 5x Q-solution and 2μL of QIAGEN RNase-free water ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... The collected worm material was incubated in 600 µL of Cell Lysis Solution (Qiagen ref. 158906) with 5 µL of proteinase K (20mg/ml ...
-
bioRxiv - Cell Biology 2020Quote: ... 3 μL of 10 μM double-stranded mature miRNA mimic (Dharmacon) or 20 μM LNA (Qiagen) was mixed with 3 μL lipofectamine RNAiMAX in 100 μL Opti-MEM reduced serum media and added to cells following a 15-minute incubation ...
-
bioRxiv - Plant Biology 2020Quote: Total DNA was isolated from 2-3 weeks old seedlings with DNeasy Plant Mini Kit (QIAGEN). 1ng of DNA per qPCR reaction was used as template ...
-
bioRxiv - Developmental Biology 2022Quote: The total RNA of 3-4 organoids was extracted using the RNeasy Plus Micro Kit (Qiagen), followed by reverse transcription to generate cDNA with GoScript Reverse Transcription Kit (Promega) ...
-
bioRxiv - Cell Biology 2021Quote: Total RNA samples obtained from 3 biological replicates were purified using RNeasy Mini Kit (#74104, Qiagen) according the manufacturer instructions with a DNase digestion step (#79254 ...
-
bioRxiv - Cancer Biology 2020Quote: RNA was isolated from control and GDC-RPC-3 cells using the RNeasy PlusMini Kit (Qiagen). High-quality (Agilent Bioanalyzer RIN>7.0 ...
-
bioRxiv - Microbiology 2022Quote: ... The soluble extracts were mixed for 30 min with 3 ml of Ni2+-NTA-agarose (Qiagen) that had been equilibrated with buffer A containing 10 mM imidazole ...
-
bioRxiv - Microbiology 2020Quote: RNA was extracted from 3 million transfected cells using the All Prep DNA/RNA kit (Qiagen) and quantified by Nanodrop 1000 ...
-
bioRxiv - Microbiology 2020Quote: The 3 Kb mutagenic DNA fragments were purified using a QIAquick PCR Purification Kit (Qiagen; 28106) and transformed into V ...
-
bioRxiv - Biochemistry 2020Quote: ... and pJET_Luc_FL_UTR_REV (5’-GCAATGAAAATAAATGTTTTTTATTAGGCAGAATCCAAATGC-3’) primers and was purified with the QIAquick PCR Purification Kit (Qiagen) directly after the PCR reaction ...
-
bioRxiv - Genetics 2021Quote: RNA was extracted from an aliquot of 3 × 106 cells using the RNeasy Plus Kit (Qiagen). cDNA was synthesized from extracted RNA using the iScript cDNA Synthesis Kit (BioRad) ...
-
bioRxiv - Cell Biology 2021Quote: ... Media and osteoblasts were harvested 3 days later and EVs were collected using Exoeasy kit (Qiagen). Total RNA was extracted using miRNeasy micro (Qiagen).
-
bioRxiv - Cancer Biology 2021Quote: ... RNA from 3 x 106 HMC-1.2 cells was extracted using RNeasy Plus Mini Kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2022Quote: ... 3 min in 37°C shaker) and processed with DNeasy Blood and Tissue Kit (QIAGEN #69504). Two hundred ng of genomic DNA was used as input in the DamID protocol that included an improved pool of AdR primers as described (de la Cruz Ruiz et al ...
-
bioRxiv - Microbiology 2023Quote: ... and the supernatant was added to 3 ml of Ni-nitrilotriacetic acid (NTA) superflow resin (Qiagen) and the mixture was applied onto a gravity drip column ...
-
bioRxiv - Cancer Biology 2024Quote: ... and total RNA was extracted using the RNAeasy Mini kit (Qiagen, 74104; n = 3 independent experiments). Prior to library construction ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were mechanically disrupted for 2x 3 min at 30 Hz (TissueLyser II, Qiagen, Hilden, Germany). This was followed by adding 5 μl of proteinase K (20 mg/ml ...
-
bioRxiv - Neuroscience 2022Quote: ... RNA was isolated (n = 3 or 4 mice per sample) using the RNeasy Mini Kit (QIAGEN), and aRNA was synthesized with Ambion’s MessageAmp aRNA Kit (Catalog # 1750 ...
-
bioRxiv - Cell Biology 2022Quote: ... A Locked Nucleic Acid (LNA) probe was used to detect centromere regions (5’-TTGGCTACACCATGAAAGCTT-3’; Qiagen). 20 μL of probe mix (250 nM LNA probe ...
-
bioRxiv - Microbiology 2022Quote: ... 3 mL samples were added to tubes containing 6 mL RNAprotect Bacteria Reagent (Qiagen, Hilden, Germany) and vortexed ...
-
bioRxiv - Microbiology 2023Quote: ... whole eyes were homogenized in PBS using a TissueLyser II (Qiagen, 30 Hz for 3 minutes), and homogenates were serially diluted and streaked on LB plates for quantification of colony forming units (CFU ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... The obtained 5□- and 3□-RACE products were purified using a QIAquick Gel Extraction Kit (QIAGEN), subcloned into a pTAC-2 Vector (Bio Dynamics Laboratory lnc. ...
-
bioRxiv - Plant Biology 2024Quote: ... 3-4 plants were pooled and RNA was extracted using the RNeasy plant mini kit (Qiagen). 100 ng of total RNA per sample determined using a Qubit fluorometer (Thermofisher) ...
-
bioRxiv - Microbiology 2024Quote: ... Demultiplexed raw reads were trimmed for quality and 3’ adaptors using the CLC Genomics Workbench (Qiagen). Reads were mapped to PAO1 ...
-
bioRxiv - Microbiology 2024Quote: ... whole eyes were homogenized in PBS using a TissueLyser II (Qiagen, 30 Hz for 3 minutes), and homogenates were serially diluted plated on LB agar plates for quantification of colony forming units (CFU ...
-
bioRxiv - Microbiology 2024Quote: ... and homogenized by bead-beating with 3 x 5 mm stainless steel beads (Qiagen, Hilden, Germany) in 1 ml sterile 1X PBS to produce internal fly-bacterial suspensions.
-
bioRxiv - Microbiology 2024Quote: 3-5 × 105 cells were harvested and total RNA was extracted using the RNeasy kit (Qiagen) employing on-column DNase treatment ...
-
bioRxiv - Molecular Biology 2024Quote: ... 3 independent PCR reactions were pooled and purified using the QIAquick PCR Purification Kit (Qiagen #28106).